FastQCFastQC Report
Tue 24 May 2016
ERR840968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923946
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCGTGGAGAAAGAAATGGCTCC67060.3485544812588295No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC62400.3243334272375628No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55520.2885735878241905No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC54930.2855069736884507No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA50840.2642485807813733No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA50510.26253335592579No Hit
AATTCGTGGAGAAAGAAATGGCTC49240.2559323390573332No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC48120.2501109698505052No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC38620.20073328461401724No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT33570.17448514667251577No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT32530.1690795895518897No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32210.16741634120708168No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT32170.1672084351639807No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31930.1659609989053747No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA29740.15457814304559483No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA29160.1515635054206303No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG28220.14667771340775676No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26230.13633438776348195No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG25960.13493102197255016No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23150.1203256224447048No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22930.11918213920764928No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA22510.11699912575508875No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG22180.1152839008995055No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT20860.10842300147717243No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA20220.1050965047875564No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA19790.10286151482422064No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC19610.10192593763026614No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG19530.10151012554406413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGC500.0269.931644
TGCACGA151.0312409E-5269.931644
ATTAACG352.5465852E-11231.3699644
TAAAACG302.6848284E-9224.9430244
TACCTTT406.366463E-11202.4487244
TAAGCCA19850.0191.7398444
GCACGAA451.4370016E-10179.9544244
GTATTAT150.0037155556179.954444
ACGACAC651.0913936E-11145.347844
CATCATT750.0143.9635344
AATGGCC1250.0140.3644444
ATCGGGA1550.0139.3195344
CTATCAT12500.0138.2049944
GTATTGT702.0008883E-11134.9658244
AGGGTTA1650.0130.8759344
GATCCGA400.0128.6027243
CAATGTT301.4988473E-9128.6027243
TAAGGTT203.8696744E-6128.6027243
TATCCAA203.8696744E-6128.6027243
AACGACA1900.0125.2184443
Produced by FastQC (version 0.11.5)