##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840968.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1923946
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.896158208182555	39.0	35.0	39.0	31.0	39.0
2	36.59533375676864	39.0	35.0	39.0	33.0	39.0
3	36.82731220107009	39.0	35.0	39.0	33.0	39.0
4	37.38885083053267	39.0	35.0	40.0	34.0	41.0
5	38.02487647782214	40.0	35.0	41.0	34.0	41.0
6	38.06946452759069	40.0	35.0	41.0	34.0	41.0
7	37.9767555846162	40.0	35.0	41.0	34.0	41.0
8	37.899058497483814	40.0	35.0	41.0	34.0	41.0
9	37.81644859055296	40.0	35.0	41.0	34.0	41.0
10	37.6780242272912	39.0	35.0	41.0	33.0	41.0
11	37.5207438254504	39.0	35.0	41.0	33.0	41.0
12	37.535395484072836	39.0	35.0	41.0	33.0	41.0
13	37.43341601063647	39.0	35.0	41.0	33.0	41.0
14	37.41355838469479	39.0	35.0	41.0	33.0	41.0
15	37.45030577781289	39.0	35.0	41.0	33.0	41.0
16	37.419123509703496	39.0	35.0	41.0	33.0	41.0
17	37.435767947749056	39.0	35.0	41.0	33.0	41.0
18	37.517613799971514	39.0	35.0	41.0	33.0	41.0
19	37.57083774700537	39.0	35.0	41.0	33.0	41.0
20	37.57819190351496	39.0	35.0	41.0	33.0	41.0
21	37.5119005419071	39.0	35.0	41.0	33.0	41.0
22	37.41078075995896	39.0	35.0	41.0	33.0	41.0
23	37.30909391427826	39.0	35.0	41.0	33.0	41.0
24	37.134787047037705	38.0	35.0	41.0	33.0	41.0
25	37.026196970826014	38.0	35.0	41.0	33.0	41.0
26	37.03452083275991	38.0	35.0	41.0	33.0	41.0
27	36.94831722716556	38.0	35.0	41.0	33.0	41.0
28	36.89725626898762	37.0	35.0	41.0	33.0	41.0
29	36.71774222143903	37.0	35.0	40.0	33.0	41.0
30	36.62388012591017	37.0	35.0	40.0	33.0	41.0
31	36.55483967464463	36.0	35.0	40.0	33.0	41.0
32	36.44466739386593	36.0	35.0	40.0	33.0	41.0
33	36.37259129586586	36.0	35.0	40.0	33.0	41.0
34	36.32468106367623	36.0	35.0	40.0	33.0	41.0
35	36.175971937539906	35.0	35.0	40.0	32.0	41.0
36	36.05551842207549	35.0	35.0	40.0	32.0	41.0
37	36.05826531263178	35.0	35.0	40.0	32.0	41.0
38	36.007346106784844	35.0	35.0	40.0	32.0	41.0
39	35.899632291826734	35.0	35.0	39.0	32.0	41.0
40	35.81456343331848	35.0	35.0	39.0	32.0	41.0
41	35.73732649637718	35.0	35.0	39.0	32.0	41.0
42	35.694800567924005	35.0	35.0	39.0	32.0	41.0
43	35.57358332734271	35.0	34.0	39.0	32.0	41.0
44	35.47396777564461	35.0	34.0	39.0	31.0	41.0
45	35.30964495852995	35.0	34.0	39.0	31.0	41.0
46	35.05035982184121	35.0	34.0	38.0	30.0	41.0
47	34.917938347889724	35.0	34.0	38.0	30.0	40.0
48	35.001344651360455	35.0	34.0	38.0	30.0	40.0
49	35.138897219412435	35.0	34.0	38.0	31.0	41.0
50	35.604742651076386	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	31.0
22	127.0
23	259.0
24	621.0
25	1406.0
26	2655.0
27	5143.0
28	9317.0
29	16142.0
30	27369.0
31	46704.0
32	77277.0
33	143524.0
34	324125.0
35	111812.0
36	156062.0
37	223346.0
38	246872.0
39	270736.0
40	260406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.107518610189686	17.8067367795146	42.08574461029572
2	24.66098320846843	20.353793713544974	32.01368437575691	22.971538702229687
3	24.10592604989953	17.262386782165404	28.19476222305616	30.436924944878914
4	19.832365357447664	19.75076223553052	33.106022726209574	27.31084968081225
5	22.57599745523003	20.554475021648216	31.680151106112124	25.189376417009623
6	25.979159498239557	23.040667461560773	25.39000574860209	25.59016729159758
7	31.976001405444855	19.270447299456432	20.99851035320118	27.755040941897533
8	27.12633306756011	19.73501335276562	24.005611384103297	29.13304219557098
9	28.264670630048865	21.972965977215576	22.995551850207853	26.7668115425277
10	30.123974373501127	18.733841802212744	22.44382118832857	28.698362635957558
11	29.191879605768563	21.21925459446367	22.7197125075236	26.869153292244167
12	31.29729212774163	20.00191273559653	22.63982461046204	26.0609705261998
13	27.37296161118867	19.318681501455863	26.190807850116375	27.11754903723909
14	23.400656775190157	20.643718690649322	22.504061964317085	33.45156256984343
15	25.61688321813606	22.620489348453646	24.588372022915404	27.17425541049489
16	27.391621178556985	23.649000543674305	20.470688886278513	28.488689391490198
17	26.69482407510398	24.185190228831786	20.410032298203795	28.70995339786044
18	26.574342523126948	22.46856200745759	19.00089711457598	31.956198354839483
19	28.37298967850449	22.1541560937781	23.614280234476436	25.858573993240974
20	31.161529481596677	19.95617340611431	21.235159406760896	27.64713770552812
21	29.26646589873105	23.145504083794453	20.131386223937678	27.456643793536823
22	28.504386297744322	23.256110098724182	21.893649821772545	26.345853781758947
23	31.044842214906236	22.87808493585579	21.51463710519942	24.562435744038556
24	30.223873227211158	22.818675784039677	22.230301682063843	24.727149306685323
25	30.340018534825212	24.571185378688728	20.604984671510632	24.483811414975428
26	30.44283509312759	23.822645767918885	21.28675295198694	24.447766186966582
27	27.463541782983082	25.64709147814102	21.048884266363793	25.840482472512104
28	28.30045174766269	21.569881921327923	22.504911927378302	27.624754403631087
29	32.62321539283116	22.434555001950216	21.230149989201294	23.712079616017327
30	28.386754354105054	25.78931666061329	20.362521402999015	25.461407582282643
31	26.621596264559273	24.2195577625428	23.878320792122288	25.280525180775644
32	26.515110901533795	20.858766160945898	26.01522523301193	26.61089770450838
33	27.12490365735175	20.808191373326178	23.94163773398781	28.125267235334263
34	27.684117026591903	22.356853383667353	25.241048131904737	24.717981457836007
35	26.56319221098018	21.220699136930747	21.59298818384509	30.623120468243982
36	24.97983558418391	22.643347090819628	27.12816030879079	25.248657016205673
37	23.935684660186283	23.444659779823496	26.05835958850557	26.561295971484654
38	26.03348275754384	23.661300075800376	22.73386686955806	27.571350297097723
39	28.181458299653883	25.784985830353836	20.788564658543233	25.244991211449054
40	27.286660821823304	25.61150955056987	21.958118343705536	25.143711283901286
41	26.918629042131766	24.100270457049785	21.747102639639916	27.233997861178533
42	27.08195377567002	24.833735856789474	21.665598149855825	26.418712217684686
43	26.892317965858815	21.695866234708326	24.29159683386338	27.12021896556948
44	26.506415708765708	23.094607402367007	22.9791732484386	27.419803640428686
45	28.249810942047844	22.359404494897213	24.60026594044303	24.790518622611913
46	26.984135045464736	23.96444659843389	23.00552628886885	26.045892067232522
47	28.518279560580407	20.95253766113423	24.083120797086703	26.44606198119866
48	26.06487888330736	23.03156770657397	20.410042388118253	30.493511022000412
49	26.59187833256241	23.296379695661972	20.610336513616595	29.50140545815903
50	23.493289998272708	29.507439694586523	22.656082007607136	24.34318829953363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39482.0
1	29083.5
2	18765.5
3	16284.0
4	11567.0
5	7995.0
6	4942.5
7	3398.0
8	3590.5
9	4113.5
10	4819.5
11	4743.5
12	4096.5
13	3866.0
14	3992.5
15	4168.0
16	4084.0
17	3881.5
18	3953.5
19	3787.0
20	3538.0
21	4008.0
22	4852.5
23	6519.5
24	8161.0
25	8360.0
26	8575.0
27	10071.0
28	10977.5
29	10865.5
30	11873.5
31	16032.0
32	22515.5
33	26516.5
34	30538.0
35	34100.0
36	32927.0
37	30245.5
38	28271.0
39	34177.0
40	50023.0
41	64667.0
42	74692.0
43	87526.5
44	93869.5
45	82233.0
46	72601.5
47	77514.5
48	87706.0
49	97922.0
50	105483.5
51	110020.0
52	112610.0
53	116899.0
54	126579.0
55	126055.0
56	118069.5
57	117213.5
58	119965.5
59	125218.0
60	127764.0
61	125571.5
62	123302.0
63	120545.0
64	117570.5
65	116145.5
66	116491.5
67	115185.0
68	104056.0
69	92460.5
70	83383.0
71	77980.5
72	76037.0
73	65846.5
74	53933.0
75	48727.5
76	44386.0
77	34818.5
78	26495.0
79	20525.5
80	15463.5
81	11620.5
82	8538.5
83	7232.0
84	6825.5
85	6316.5
86	5163.5
87	3875.5
88	2386.0
89	1605.0
90	1109.5
91	514.5
92	346.5
93	355.5
94	247.5
95	112.0
96	76.5
97	70.0
98	35.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	18344.0
25	16366.0
26	8590.0
27	9690.0
28	9622.0
29	11126.0
30	11603.0
31	10587.0
32	15477.0
33	11241.0
34	11293.0
35	9643.0
36	8967.0
37	10192.0
38	10632.0
39	11148.0
40	16046.0
41	15404.0
42	24788.0
43	37227.0
44	97493.0
45	170146.0
46	223616.0
47	236995.0
48	327511.0
49	306518.0
50	283681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.715626106623786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72807447826254	10.702391514767992
2	10.86737282813287	3.1984039060017206
3	4.233267143526177	1.8688552948075956
4	2.162417978035908	1.272853378040713
5	1.3202030754016816	0.9713807421212998
6	0.9245823549423167	0.8163484944067714
7	0.6452319086454752	0.6646494063783035
8	0.5507052859389586	0.6483178466255245
9	0.3889440266774295	0.5151199385690783
>10	3.225237903986175	10.37164762299202
>50	1.401028912982937	15.303045751459146
>100	1.4676639036044534	37.37065050171832
>500	0.05703503434553667	5.676535572497723
>1k	0.024846945655481317	7.783942132570074
>5k	0.003388219862111089	2.8358578970437316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATTCGTGGAGAAAGAAATGGCTCC	6706	0.3485544812588295	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	6240	0.3243334272375628	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5552	0.2885735878241905	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	5493	0.2855069736884507	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	5084	0.2642485807813733	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	5051	0.26253335592579	No Hit
AATTCGTGGAGAAAGAAATGGCTC	4924	0.2559323390573332	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	4812	0.2501109698505052	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	3862	0.20073328461401724	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	3357	0.17448514667251577	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	3253	0.1690795895518897	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3221	0.16741634120708168	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	3217	0.1672084351639807	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3193	0.1659609989053747	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	2974	0.15457814304559483	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	2916	0.1515635054206303	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	2822	0.14667771340775676	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2623	0.13633438776348195	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	2596	0.13493102197255016	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2315	0.1203256224447048	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2293	0.11918213920764928	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	2251	0.11699912575508875	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG	2218	0.1152839008995055	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	2086	0.10842300147717243	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	2022	0.1050965047875564	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	1979	0.10286151482422064	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	1961	0.10192593763026614	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	1953	0.10151012554406413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.021726181504054688	0.0	0.0	0.0	0.0
2	0.03835866495213483	0.0	0.0	0.0	0.0
3	0.05098895707052069	0.0	0.0	0.0	0.0
4	0.06325541361347979	0.0	0.0	0.0	0.0
5	0.07401455134395664	0.0	0.0	0.0	0.0
6	0.09210237709374379	0.0	0.0	0.0	0.0
7	0.10421290410437715	0.0	0.0	0.0	0.0
8	0.11767482039516701	0.0	0.0	0.0	0.0
9	0.1281740755717676	0.0	0.0	0.0	0.0
10	0.1403365790931762	0.0	0.0	0.0	0.0
11	0.1507318812482263	0.0	0.0	0.0	0.0
12	0.15987974714467038	0.0	0.0	0.0	0.0
13	0.16996319023506898	0.0	0.0	0.0	0.0
14	0.1810341870301973	0.0	0.0	0.0	0.0
15	0.19345657310548217	0.0	0.0	0.0	0.0
16	0.20572302964844127	0.0	0.0	0.0	0.0
17	0.21528670763108737	0.0	0.0	0.0	0.0
18	0.23363441593475076	0.0	0.0	0.0	0.0
19	0.24512122481608112	0.0	0.0	0.0	0.0
20	0.2559323390573332	0.0	0.0	0.0	0.0
21	0.26939425534812306	0.0	0.0	0.0	0.0
22	0.2813488528264307	0.0	0.0	0.0	0.0
23	0.2927836851969858	0.0	0.0	0.0	0.0
24	0.3039586350136646	0.0	0.0	0.0	0.0
25	0.3174725278152297	0.0	0.0	0.0	0.0
26	0.32849154809958286	0.0	0.0	0.0	0.0
27	0.34023823953478943	0.0	0.0	0.0	0.0
28	0.35354422629325355	0.0	0.0	0.0	0.0
29	0.3660705653900889	0.0	0.0	0.0	0.0
30	0.3784409749545985	0.0	0.0	0.0	0.0
31	0.3910712670729844	0.0	0.0	0.0	0.0
32	0.404273300809898	0.0	0.0	0.0	0.0
33	0.4160719687558798	0.0	0.0	0.0	0.0
34	0.4290141199389172	0.0	0.0	0.0	0.0
35	0.43987721069094454	0.0	0.0	0.0	0.0
36	0.45016855982444415	0.0	0.0	0.0	0.0
37	0.45812096597305746	0.0	0.0	0.0	0.0
38	0.46295478147515573	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTAGC	50	0.0	269.9316	44
TGCACGA	15	1.0312409E-5	269.9316	44
ATTAACG	35	2.5465852E-11	231.36996	44
TAAAACG	30	2.6848284E-9	224.94302	44
TACCTTT	40	6.366463E-11	202.44872	44
TAAGCCA	1985	0.0	191.73984	44
GCACGAA	45	1.4370016E-10	179.95442	44
GTATTAT	15	0.0037155556	179.9544	44
ACGACAC	65	1.0913936E-11	145.3478	44
CATCATT	75	0.0	143.96353	44
AATGGCC	125	0.0	140.36444	44
ATCGGGA	155	0.0	139.31953	44
CTATCAT	1250	0.0	138.20499	44
GTATTGT	70	2.0008883E-11	134.96582	44
AGGGTTA	165	0.0	130.87593	44
GATCCGA	40	0.0	128.60272	43
CAATGTT	30	1.4988473E-9	128.60272	43
TAAGGTT	20	3.8696744E-6	128.60272	43
TATCCAA	20	3.8696744E-6	128.60272	43
AACGACA	190	0.0	125.21844	43
>>END_MODULE