FastQCFastQC Report
Tue 24 May 2016
ERR840967.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840967.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13891577
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA319580.23005307460772814No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG152760.10996591675660726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT135850.0216.806844
ACATTGG20250.0103.5268444
CCTATCA135050.091.8499943
ACTTCTA38150.089.78071644
AACGACA13200.088.9430443
CATGACC20150.082.060244
ACCACTC13450.076.8361244
CCCTATC179700.071.22697442
ACGGACA2400.067.66608444
GCTATAC1802.401066E-1065.6156144
CCCATAC24200.062.8364344
CCAGAAT14500.062.10856244
AATCGCA16100.061.43821744
TGTGTTA35750.059.92093342
CGGATAC1001.3083263E-759.0540444
TGCACCG24750.054.88701243
TCGCAAA15800.054.85479443
TGGTCAT24400.054.6666543
AACCTTA40900.052.9443841
CTATACG1400.052.7268244
Produced by FastQC (version 0.11.5)