##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840967.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13891577
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.37991273416978	39.0	37.0	39.0	35.0	39.0
2	37.86464978022293	39.0	38.0	39.0	35.0	39.0
3	38.065197133485995	39.0	38.0	39.0	35.0	40.0
4	38.72093038824894	39.0	38.0	41.0	35.0	41.0
5	39.26373917086591	40.0	39.0	41.0	35.0	41.0
6	39.22071792137063	40.0	38.0	41.0	35.0	41.0
7	39.21098007807177	40.0	38.0	41.0	35.0	41.0
8	39.12637643659895	40.0	38.0	41.0	35.0	41.0
9	39.007709707832305	40.0	38.0	41.0	35.0	41.0
10	38.90542369667605	40.0	38.0	41.0	35.0	41.0
11	38.7668090527087	40.0	38.0	41.0	35.0	41.0
12	38.721475394766195	40.0	38.0	41.0	35.0	41.0
13	38.62198114728083	40.0	38.0	41.0	35.0	41.0
14	38.57594238580688	40.0	38.0	41.0	35.0	41.0
15	38.586021083135485	40.0	38.0	41.0	35.0	41.0
16	38.54790942741778	40.0	38.0	41.0	35.0	41.0
17	38.55317484832715	40.0	38.0	41.0	35.0	41.0
18	38.61137407221657	40.0	38.0	41.0	35.0	41.0
19	38.72439975677347	40.0	38.0	41.0	35.0	41.0
20	38.68563223599452	40.0	38.0	41.0	35.0	41.0
21	38.636034051425554	40.0	38.0	41.0	35.0	41.0
22	38.53839697249636	40.0	37.0	41.0	35.0	41.0
23	38.423796736684395	40.0	37.0	41.0	34.0	41.0
24	38.22418916153292	40.0	37.0	41.0	34.0	41.0
25	38.163566804330316	40.0	37.0	41.0	34.0	41.0
26	38.08260706479912	40.0	36.0	41.0	33.0	41.0
27	37.994733781976336	40.0	36.0	41.0	33.0	41.0
28	37.908155341231584	40.0	35.0	41.0	33.0	41.0
29	37.755488202944804	39.0	35.0	41.0	33.0	41.0
30	37.63675314966142	39.0	35.0	41.0	33.0	41.0
31	37.47949859160779	39.0	35.0	41.0	33.0	41.0
32	37.34245556411604	39.0	35.0	41.0	33.0	41.0
33	37.22199764648199	39.0	35.0	41.0	33.0	41.0
34	37.13807069830046	38.0	35.0	41.0	33.0	41.0
35	36.980109725171495	38.0	35.0	40.0	33.0	41.0
36	36.88322444135279	38.0	35.0	40.0	32.0	41.0
37	36.78206779446149	38.0	35.0	40.0	32.0	41.0
38	36.72729385494905	37.0	35.0	40.0	32.0	41.0
39	36.670799517442795	37.0	35.0	40.0	32.0	41.0
40	36.5715368346299	37.0	35.0	40.0	32.0	41.0
41	36.45215003327724	37.0	35.0	40.0	32.0	41.0
42	36.338603067710075	36.0	35.0	40.0	32.0	41.0
43	36.209182936154846	36.0	35.0	40.0	32.0	41.0
44	36.053467346804645	36.0	35.0	40.0	31.0	41.0
45	35.882540687468904	35.0	34.0	40.0	31.0	41.0
46	35.64063339236496	35.0	34.0	39.0	31.0	41.0
47	35.462048045540705	35.0	34.0	39.0	30.0	41.0
48	35.531544679975966	35.0	34.0	39.0	30.0	41.0
49	35.511008980302236	35.0	34.0	39.0	30.0	41.0
50	36.11256643158055	37.0	34.0	39.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	35.0
21	88.0
22	198.0
23	489.0
24	1191.0
25	2531.0
26	5355.0
27	11302.0
28	24161.0
29	48824.0
30	95174.0
31	175609.0
32	299111.0
33	514767.0
34	919740.0
35	867424.0
36	1361396.0
37	2015832.0
38	2283152.0
39	2747807.0
40	2517380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.45617491808165	18.32803431892578	38.21579076299257
2	26.934436601402417	18.341409330272583	30.692267695741094	24.031886372583905
3	23.801566949526322	17.343948782776785	29.94115786854149	28.9133263991554
4	21.650788819728675	18.500923257309086	33.72674679051917	26.12154113244306
5	22.743285373575656	18.637624799545797	30.205764255562922	28.413325571315625
6	24.106780677240604	20.385633682914474	28.02790496716104	27.479680672683887
7	28.99074741478235	18.208011948535432	21.666345008921592	31.134895627760624
8	27.58989134207009	19.783894945836604	23.04179719840303	29.584416513690275
9	27.826387169721624	19.234590860346525	25.59464630977462	27.344375660157233
10	29.471470373737986	17.384520130435874	23.888266969257703	29.25574252656844
11	28.996923819376303	18.743307545284456	23.5271560601075	28.73261257523174
12	29.41131161710438	20.22395297524536	22.15302841426859	28.211706993381675
13	30.629445454608934	17.566846442272176	24.33748882506284	27.466219278056048
14	27.54477767354995	19.271836451685793	22.16588512592919	31.01750074883507
15	26.743047243664268	21.074741910151744	22.93411323998708	29.248097606196904
16	28.77946830658607	20.761969645346962	21.024654004365377	29.43390804370159
17	29.051568443237226	22.91991758747045	19.13600594086618	28.892508028426146
18	26.995451992239616	20.660476488738464	20.237745505783828	32.106326013238096
19	29.908886514468442	19.116987221825138	21.579047504829727	29.395078758876693
20	29.11087776427399	19.63596357706544	23.355181344781805	27.897977313878762
21	28.947397404916664	23.83039017096475	19.565741168191344	27.656471255927244
22	31.78015714126625	21.11727847745436	21.11655141817232	25.98601296310707
23	32.562638496694795	20.725811043627374	21.338837196093717	25.372713263584114
24	31.525002524911315	21.33684318202318	22.4265394778433	24.7116148152222
25	31.218963355167368	22.22054980021271	20.666154953774697	25.89433189084523
26	31.19261317238649	22.09807590995742	22.17905217546705	24.530258742189044
27	30.206734556039716	23.33134604447912	21.065829442675827	25.396089956805334
28	31.28093369214446	21.088695613864402	20.73921455608137	26.891156137909768
29	30.14508371287134	23.137321639769493	21.223459706930782	25.49413494042839
30	31.020039805525606	23.254567812192793	20.201144548132262	25.52424783414934
31	29.57866405881237	22.55018599412232	21.59329051199533	26.277859435069978
32	28.33646304111931	21.745357107079023	24.087508675387532	25.83067117641414
33	27.393627898164556	20.323384513384088	23.781055902388413	28.501931686062942
34	29.686434002510836	20.807239262387725	23.45133145773183	26.054995277369606
35	26.832456150138572	20.184829854419267	23.587187418118774	29.395526577323388
36	28.256342483666202	20.71357731640301	24.942077827765637	26.088002372165146
37	26.558289428990996	24.315286155496516	23.749467953970175	25.37695646154232
38	28.111528099178674	21.458229653693078	22.591554137257717	27.838688109870528
39	27.928831103163066	21.837513650780217	22.397575623012884	27.836079623043837
40	27.369666165491452	21.965246853893053	23.27048258651233	27.394604394103165
41	27.054269127367604	22.190499235488208	21.98110723855266	28.774124398591532
42	26.364691243388584	23.067442096229808	22.22578805886687	28.342078601514743
43	26.7620010421312	21.71085709004376	22.873114239754514	28.654027628070526
44	26.472111487411436	23.054980681775973	21.73983417876962	28.733073652042968
45	27.38736570212022	20.36591772017443	24.95892539925495	27.287791178450398
46	27.37770045862421	22.659448668244746	22.882154830016812	27.080696043114227
47	26.423468713566205	21.851716722388083	23.858471116661814	27.8663434473839
48	25.57957231719109	22.69240235504405	21.55922415565933	30.168801172105532
49	26.103571859579116	24.410035883564973	20.833659027523282	28.65273322933263
50	21.916389623755443	26.09931054215002	26.918588885401384	25.065710948693148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102126.0
1	80844.0
2	67470.5
3	66349.0
4	50542.5
5	34928.0
6	23122.0
7	24049.0
8	31091.0
9	35433.5
10	37134.0
11	37204.0
12	35246.0
13	32747.5
14	32603.0
15	33835.0
16	34713.0
17	35136.5
18	34881.5
19	35202.5
20	36387.5
21	39700.0
22	43934.5
23	50515.5
24	58243.0
25	66079.5
26	76699.5
27	87281.5
28	95257.5
29	104779.0
30	119714.5
31	142463.5
32	174108.5
33	194992.5
34	212742.5
35	229320.0
36	231920.5
37	239712.0
38	253935.0
39	281873.0
40	318544.5
41	362790.0
42	410545.5
43	420176.0
44	406749.5
45	411055.0
46	460144.0
47	524515.0
48	584976.5
49	676238.0
50	783682.0
51	855812.0
52	894914.5
53	885955.0
54	867905.5
55	880460.5
56	889230.0
57	908228.5
58	936075.5
59	946427.5
60	955431.0
61	967725.0
62	941808.0
63	914831.0
64	893678.0
65	857320.0
66	808352.5
67	771888.5
68	755714.5
69	725665.0
70	666008.0
71	610328.0
72	566955.5
73	531651.5
74	490228.0
75	434966.0
76	377954.0
77	299356.0
78	242082.0
79	211599.0
80	173570.0
81	129210.0
82	95325.0
83	73052.0
84	57360.5
85	46151.0
86	33499.5
87	24208.5
88	15682.0
89	9120.0
90	6272.5
91	4112.5
92	3074.5
93	2503.0
94	1653.5
95	592.0
96	295.0
97	263.0
98	140.0
99	31.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	72630.0
25	70474.0
26	64839.0
27	64584.0
28	68804.0
29	87291.0
30	75704.0
31	91015.0
32	116817.0
33	94550.0
34	89674.0
35	78725.0
36	74536.0
37	88305.0
38	88602.0
39	81936.0
40	94075.0
41	99840.0
42	141198.0
43	212969.0
44	643541.0
45	1317207.0
46	1522791.0
47	1831256.0
48	2204219.0
49	2642639.0
50	1873356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.252540414789603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.02133456547557	12.598035257327403
2	12.563358172131425	4.334996892203493
3	4.227621547674584	2.188116348290734
4	1.9354339202530995	1.335646077172851
5	1.0387143525279119	0.8960230673204908
6	0.710140263432236	0.7351034157020393
7	0.5122905045897902	0.6186818844183821
8	0.38327715119720696	0.5290003632876191
9	0.2990385913326741	0.4643257844293841
>10	2.9292953380080258	12.078045215167418
>50	1.3660604217873042	17.075807962125204
>100	0.932318238007091	27.728339581129436
>500	0.04872460104493409	5.754652480342124
>1k	0.029806390024694318	9.515548789319574
>5k	0.0019054313257795454	2.247305019406648
>10k+	6.805111877784091E-4	1.9003718623571826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	31958	0.23005307460772814	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	15276	0.10996591675660726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02862166044934999	0.0	0.0	0.0	0.0
2	0.04902251198694	0.0	0.0	0.0	0.0
3	0.06143291002886137	0.0	0.0	0.0	0.0
4	0.07581572632106491	0.0	0.0	0.0	0.0
5	0.08716792917031665	0.0	0.0	0.0	0.0
6	0.10492689202960902	0.0	0.0	0.0	0.0
7	0.11802835631980443	0.0	0.0	0.0	0.0
8	0.13010041984434165	0.0	0.0	0.0	0.0
9	0.1422948596836774	0.0	0.0	0.0	0.0
10	0.15330872801554496	0.0	0.0	0.0	0.0
11	0.16423621306637828	0.0	0.0	0.0	0.0
12	0.17643065290571402	0.0	0.0	0.0	0.0
13	0.18750930869835727	0.0	0.0	0.0	0.0
14	0.1983432118614035	0.0	0.0	0.0	0.0
15	0.21239489224297572	0.0	0.0	0.0	0.0
16	0.22379748533949745	0.0	0.0	0.0	0.0
17	0.23675497749463578	0.0	0.0	0.0	1.4397213505709251E-5
18	0.26239641474830394	0.0	0.0	0.0	1.4397213505709251E-5
19	0.2747348267226968	0.0	0.0	0.0	1.4397213505709251E-5
20	0.2863317822015456	0.0	0.0	0.0	1.4397213505709251E-5
21	0.2998939573239237	0.0	0.0	0.0	1.4397213505709251E-5
22	0.3125707038157007	0.0	0.0	0.0	1.4397213505709251E-5
23	0.3253914224425348	0.0	0.0	0.0	1.4397213505709251E-5
24	0.33782341630471474	0.0	0.0	0.0	1.4397213505709251E-5
25	0.35124161929203573	0.0	0.0	0.0	1.4397213505709251E-5
26	0.3686694462406968	0.0	0.0	0.0	1.4397213505709251E-5
27	0.38187169102543217	0.0	0.0	0.0	1.4397213505709251E-5
28	0.39546985918157457	0.0	0.0	0.0	1.4397213505709251E-5
29	0.4097734907994967	0.0	0.0	0.0	1.4397213505709251E-5
30	0.42445144996856726	0.0	0.0	0.0	1.4397213505709251E-5
31	0.43870469133921947	0.0	0.0	0.0	1.4397213505709251E-5
32	0.4536705947784042	0.0	0.0	0.0	1.4397213505709251E-5
33	0.4677294737667293	0.0	0.0	0.0	1.4397213505709251E-5
34	0.4792616417848024	0.0	0.0	0.0	1.4397213505709251E-5
35	0.4898651895317573	0.0	0.0	0.0	1.4397213505709251E-5
36	0.4997416779966738	0.0	0.0	0.0	1.4397213505709251E-5
37	0.5078545078071409	0.0	0.0	0.0	1.4397213505709251E-5
38	0.513253462871782	0.0	0.0	0.0	1.4397213505709251E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	13585	0.0	216.8068	44
ACATTGG	2025	0.0	103.52684	44
CCTATCA	13505	0.0	91.84999	43
ACTTCTA	3815	0.0	89.780716	44
AACGACA	1320	0.0	88.94304	43
CATGACC	2015	0.0	82.0602	44
ACCACTC	1345	0.0	76.83612	44
CCCTATC	17970	0.0	71.226974	42
ACGGACA	240	0.0	67.666084	44
GCTATAC	180	2.401066E-10	65.61561	44
CCCATAC	2420	0.0	62.83643	44
CCAGAAT	1450	0.0	62.108562	44
AATCGCA	1610	0.0	61.438217	44
TGTGTTA	3575	0.0	59.920933	42
CGGATAC	100	1.3083263E-7	59.05404	44
TGCACCG	2475	0.0	54.887012	43
TCGCAAA	1580	0.0	54.854794	43
TGGTCAT	2440	0.0	54.66665	43
AACCTTA	4090	0.0	52.94438	41
CTATACG	140	0.0	52.72682	44
>>END_MODULE