FastQCFastQC Report
Tue 24 May 2016
ERR840966.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840966.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872287
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT127781.4648848372152743No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC73060.8375683691262166No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC44660.5119874536706382No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA43790.5020136721056258No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC37160.4260065781101862No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31720.36364178303700506No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA31140.35699259532699673No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA29720.34071354955421784No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA28840.33062512682179146No Hit
AATTCGTGGAGAAAGAAATGGCTCC27350.31354359287711503No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26000.2980670352762336No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA25800.2957742119279549No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT20340.23318013451994585No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19470.22320635295493338No Hit
AATTCGTGGAGAAAGAAATGGCTC19400.22240386478303587No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA18530.21243008321802345No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG18360.2104811833719865No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT15300.17540098614332209No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC15000.171961751120904No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC14830.1700128512748671No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14660.16806395142883018No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC13640.15637055235260872No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13350.15304595849760458No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT12830.1470846177920799No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT12710.14570892378311268No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG12540.14376002393707576No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG12400.14215504759328065No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA12220.1400915065798298No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC12200.13986222424500191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11830.1356205010506863No Hit
ACCTCAAGAAGATGCAAATGCTACCGACGGCGAAGTCCAAAGCTGCCTGA11500.1318373425260264No Hit
ACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGT11450.13126413668895673No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC10920.1251881548160181No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA10480.12014394344980495No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10360.1187682494408377No Hit
ATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT9330.10696020919720228No Hit
ACTGCCCTCGCTCTCTGACGCCAGCACCGCCTGCAGCCGGCCAGGCTCAG8780.10065494498943581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGC400.0155.4659444
ATTAGTC201.5034984E-6155.4659444
AAATACC100.005754115155.4659444
TCCGATG201.5034984E-6155.4659444
CCAATGG100.005754115155.4659444
ACGATGA100.005754115155.4659444
TGATTGA100.005754115155.4659444
CTTGTCA357.2759576E-12155.4659344
CAGGTTA252.4418114E-8155.4659344
GTGCATA159.308504E-5155.4659344
TATGGGT500.0155.4659344
GTTAGTA357.2759576E-12155.4659344
GTAGGCA700.0155.4659344
ACCTCGC303.9835868E-10155.4659344
GAAAGTC450.0155.4659344
AAGCCTA252.4418114E-8155.4659344
ATGAGAC650.0155.4659344
ACCAGCG159.308504E-5155.4659344
AACTGGA303.9835868E-10155.4659344
TAGTGTC252.4418114E-8155.4659344
Produced by FastQC (version 0.11.5)