##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840966.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872287
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.19876485606228	39.0	38.0	39.0	34.0	39.0
2	37.656593529423226	39.0	38.0	39.0	35.0	39.0
3	37.85610928513207	39.0	38.0	39.0	35.0	39.0
4	38.16739559342281	39.0	38.0	39.0	35.0	41.0
5	39.1590439843767	40.0	39.0	41.0	35.0	41.0
6	39.21341829008113	40.0	38.0	41.0	35.0	41.0
7	39.21503358412999	40.0	38.0	41.0	35.0	41.0
8	39.2069605531207	40.0	38.0	41.0	35.0	41.0
9	39.11630690357646	40.0	38.0	41.0	35.0	41.0
10	38.932953259649636	40.0	38.0	41.0	35.0	41.0
11	38.90983242900559	40.0	38.0	41.0	35.0	41.0
12	38.92337155087718	40.0	38.0	41.0	35.0	41.0
13	38.81338366844857	40.0	38.0	41.0	35.0	41.0
14	38.832353342420554	40.0	38.0	41.0	35.0	41.0
15	38.86177943727237	40.0	38.0	41.0	35.0	41.0
16	38.838443081233585	40.0	38.0	41.0	35.0	41.0
17	38.821362693700586	40.0	38.0	41.0	35.0	41.0
18	38.82163553967903	40.0	38.0	41.0	35.0	41.0
19	38.86780383061997	40.0	38.0	41.0	35.0	41.0
20	38.8635689858957	40.0	38.0	41.0	35.0	41.0
21	38.857341677681774	40.0	38.0	41.0	35.0	41.0
22	38.82335286436689	40.0	38.0	41.0	35.0	41.0
23	38.696203199176416	40.0	38.0	41.0	35.0	41.0
24	38.51379419846908	40.0	37.0	41.0	35.0	41.0
25	38.45411956316794	40.0	37.0	41.0	35.0	41.0
26	38.51474092172102	40.0	37.0	41.0	35.0	41.0
27	38.45686543999402	40.0	37.0	41.0	35.0	41.0
28	38.43416987465955	40.0	37.0	41.0	35.0	41.0
29	38.352089626798495	40.0	36.0	41.0	34.0	41.0
30	38.36215534991598	40.0	36.0	41.0	34.0	41.0
31	38.26899027176077	40.0	35.0	41.0	34.0	41.0
32	38.22941887536351	40.0	35.0	41.0	34.0	41.0
33	38.101412258709495	40.0	35.0	41.0	34.0	41.0
34	38.09117789744336	40.0	35.0	41.0	34.0	41.0
35	37.98914282711739	40.0	35.0	41.0	34.0	41.0
36	37.971979191007364	40.0	35.0	41.0	34.0	41.0
37	37.934298969148564	40.0	35.0	41.0	34.0	41.0
38	37.86237536878923	39.0	35.0	41.0	34.0	41.0
39	37.798854097867384	39.0	35.0	41.0	33.0	41.0
40	37.76127556407639	39.0	35.0	41.0	34.0	41.0
41	37.62320634791909	39.0	35.0	41.0	33.0	41.0
42	37.629258460721	39.0	35.0	41.0	33.0	41.0
43	37.55525592504229	39.0	35.0	41.0	33.0	41.0
44	37.4247407612238	39.0	35.0	41.0	33.0	41.0
45	37.31471724285397	39.0	35.0	41.0	33.0	41.0
46	37.03219522713142	38.0	35.0	40.0	33.0	41.0
47	36.899658557555426	38.0	35.0	40.0	32.0	41.0
48	36.76784125995535	38.0	35.0	40.0	32.0	41.0
49	37.096570150014635	38.0	35.0	40.0	33.0	41.0
50	36.644367602074745	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	9.0
22	34.0
23	69.0
24	114.0
25	282.0
26	423.0
27	706.0
28	1331.0
29	2429.0
30	4697.0
31	8528.0
32	14135.0
33	26184.0
34	61308.0
35	40438.0
36	64406.0
37	92871.0
38	104712.0
39	182969.0
40	266637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.88903996047172	18.183923410528873	34.9270366289994
2	25.276543156094267	26.92771989035719	30.01087944678758	17.78485750676096
3	26.358755776481825	21.246332915657344	22.816572985726026	29.5783383221348
4	19.5612223958399	24.59775280383635	28.519741782234515	27.321283018089233
5	21.873878666081232	26.030652755343137	24.82749370333388	27.26797487524175
6	20.720932445399278	32.90384930647826	20.72505952742618	25.650158720696286
7	22.32178170716748	27.32380512377234	20.825026625411134	29.52938654364905
8	24.041513859543933	31.812236110362758	22.58121466902522	21.565035361068087
9	23.528838558868813	29.172967154159124	24.986386361369593	22.311807925602466
10	26.322987732248677	23.802143101983635	23.831835164343847	26.04303400142384
11	24.56232868310545	28.302382128817694	23.35836714292429	23.77692204515257
12	20.08398611924745	28.414730472883353	25.56669995081894	25.93458345705026
13	20.818492078868537	27.733074091440084	25.25716879880131	26.191265030890065
14	17.830026126722053	28.23325350486709	23.21793171284222	30.718788655568634
15	23.395510881166405	30.131252672572217	21.6325590086749	24.840677437586482
16	20.520425043592304	34.67872386038081	20.888881755660694	23.911969340366188
17	23.847770286614384	35.564097596318646	18.39990736993673	22.188224747130246
18	20.627729176291748	33.271044965705094	19.42949969448129	26.671726163521868
19	22.972255691074153	28.578323418783036	21.365215806265596	27.08420508387721
20	23.705615239021103	26.440494928847958	25.855824975036885	23.998064857094054
21	21.273617513501865	33.55432329038493	21.437210459401552	23.734848736711655
22	24.002535862623194	28.153233970012163	20.1370649797601	27.707165187604538
23	24.15432076827925	27.42606504510557	25.593296701658975	22.826317484956213
24	23.834357270026953	29.3257838303219	23.668242218444156	23.171616681206988
25	20.20862998946208	29.499697511963603	22.391286493764582	27.900386004809736
26	22.67956836978775	30.376337198875508	23.786127369575798	23.157967061760946
27	22.875060772654177	32.739514379744946	19.767357604996448	24.618067242604436
28	20.89945786679616	27.54534002378724	21.248642321108946	30.30655978830766
29	21.929299546163598	34.25699016452159	21.68576895344289	22.127941335871924
30	24.871488415023784	32.41118973800677	20.92348472297825	21.793837123991196
31	22.45363189578839	30.253100616260483	21.813006245802953	25.480261242148178
32	19.532990538332935	33.008079826923925	24.21060293814058	23.248326696602557
33	20.151126514792477	30.25424515730688	23.123490332702634	26.471137995198006
34	23.24212875369912	30.160093145102607	23.587663125212245	23.010114975986028
35	20.25983896924677	29.647963653826647	21.583961933297733	28.50823544362885
36	18.588628844805747	31.583303612057414	27.26346988722313	22.564597655913715
37	17.308200617078093	36.52939272895252	20.128282087196247	26.034124566773137
38	21.803774961926432	31.26836188695966	19.628709768306653	27.299153382807255
39	19.14298306616842	32.54409500824655	24.871245197836046	23.441676727748987
40	21.082162869328634	32.44843869078783	24.619983703274034	21.8494147366095
41	21.51938994287684	32.70478425488994	19.929369009544182	25.84645679268905
42	21.677021172239026	30.975511934930104	19.174951974168234	28.172514918662632
43	20.263009965520904	30.03670689659639	21.3631806771995	28.337102460683212
44	21.120174866841797	30.878218675726032	22.13013621968964	25.871470237742532
45	20.101402751772973	29.187763684162917	28.462400934254063	22.248432629810054
46	21.350456862087757	33.17511397553458	22.087550341963524	23.386878820414136
47	18.524573989767298	34.272505602615965	23.886700305853502	23.31622010176324
48	21.48074875997061	27.277186512225253	21.256454564142643	29.98561016366149
49	19.817855108585167	32.1717977524554	20.657773414020877	27.352573724938555
50	17.432282661701468	35.18375670523562	27.831271888992333	19.552688744070576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22350.0
1	19040.0
2	15322.5
3	13451.5
4	9993.0
5	7319.5
6	6030.5
7	4777.5
8	3806.5
9	3248.5
10	3106.0
11	4175.0
12	5282.5
13	5344.5
14	5523.0
15	5680.5
16	5234.0
17	4629.5
18	4409.5
19	4233.5
20	3918.0
21	3941.0
22	4439.5
23	5917.0
24	7454.0
25	8329.0
26	10135.0
27	12235.5
28	12559.0
29	12534.0
30	14109.5
31	18828.5
32	24229.0
33	26147.5
34	27293.0
35	27335.5
36	27787.0
37	30515.0
38	31971.0
39	34424.0
40	39530.5
41	47706.0
42	52396.5
43	49476.5
44	46115.5
45	45119.5
46	46067.0
47	46123.0
48	47925.5
49	54416.5
50	60096.5
51	61199.0
52	57881.5
53	51469.5
54	46812.0
55	42653.0
56	40938.0
57	41724.5
58	40324.5
59	37493.0
60	34059.5
61	35381.0
62	39269.0
63	38703.0
64	33978.5
65	30495.5
66	26331.5
67	21653.0
68	21282.5
69	21889.0
70	20156.0
71	17689.5
72	17950.0
73	18959.0
74	16235.5
75	11826.0
76	9165.0
77	7069.0
78	5190.5
79	4826.0
80	4706.0
81	3566.0
82	2340.0
83	1663.0
84	1166.5
85	872.0
86	629.5
87	403.5
88	223.0
89	122.0
90	79.0
91	34.5
92	30.5
93	41.0
94	51.0
95	57.5
96	51.5
97	44.0
98	22.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	7790.0
25	5799.0
26	3050.0
27	3090.0
28	3794.0
29	3704.0
30	3695.0
31	3352.0
32	8774.0
33	4719.0
34	6167.0
35	5243.0
36	3488.0
37	3945.0
38	4736.0
39	4462.0
40	7307.0
41	6260.0
42	10850.0
43	12627.0
44	29869.0
45	52447.0
46	109527.0
47	76273.0
48	173550.0
49	92199.0
50	225570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.664708977664462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.00275194534068	6.47562098254359
2	12.813152400835074	2.4767077808106737
3	5.379341431011577	1.5596930826665996
4	2.6807743404820648	1.0363561534219814
5	1.5740652875308407	0.7606441457914654
6	1.0141867527044979	0.5881091888334917
7	0.7698329853862212	0.5208148235615112
8	0.5847883848927691	0.45214476428056366
9	0.4590529512241412	0.3992951861027392
>10	3.4363731258303285	7.34242284936036
>50	1.524245587397988	10.848608313548178
>100	2.6096033402922756	50.693292459935776
>500	0.11031505029417348	7.0864291225250415
>1k	0.03914405010438413	7.457407940276537
>5k	0.0011861833364964888	0.8375683691262166
>10k+	0.0011861833364964888	1.4648848372152743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	12778	1.4648848372152743	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7306	0.8375683691262166	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	4466	0.5119874536706382	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4379	0.5020136721056258	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	3716	0.4260065781101862	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3172	0.36364178303700506	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	3114	0.35699259532699673	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2972	0.34071354955421784	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	2884	0.33062512682179146	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	2735	0.31354359287711503	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2600	0.2980670352762336	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2580	0.2957742119279549	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	2034	0.23318013451994585	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1947	0.22320635295493338	No Hit
AATTCGTGGAGAAAGAAATGGCTC	1940	0.22240386478303587	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	1853	0.21243008321802345	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1836	0.2104811833719865	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	1530	0.17540098614332209	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1500	0.171961751120904	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	1483	0.1700128512748671	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1466	0.16806395142883018	No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	1364	0.15637055235260872	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1335	0.15304595849760458	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	1283	0.1470846177920799	No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	1271	0.14570892378311268	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	1254	0.14376002393707576	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG	1240	0.14215504759328065	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	1222	0.1400915065798298	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	1220	0.13986222424500191	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1183	0.1356205010506863	No Hit
ACCTCAAGAAGATGCAAATGCTACCGACGGCGAAGTCCAAAGCTGCCTGA	1150	0.1318373425260264	No Hit
ACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGT	1145	0.13126413668895673	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	1092	0.1251881548160181	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA	1048	0.12014394344980495	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1036	0.1187682494408377	No Hit
ATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	933	0.10696020919720228	No Hit
ACTGCCCTCGCTCTCTGACGCCAGCACCGCCTGCAGCCGGCCAGGCTCAG	878	0.10065494498943581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.014215504759328066	0.0	0.0	0.0	0.0
2	0.0356534030657341	0.0	0.0	0.0	0.0
3	0.04895177848575068	0.0	0.0	0.0	0.0
4	0.05972804822266066	0.0	0.0	0.0	0.0
5	0.07222393547077968	0.0	0.0	0.0	0.0
6	0.09618393946029231	0.0	0.0	0.0	0.0
7	0.11716327309704261	0.0	0.0	0.0	0.0
8	0.13711083622706746	0.0	0.0	0.0	0.0
9	0.15488021717622755	0.0	0.0	0.0	0.0
10	0.16737610442434658	0.0	0.0	0.0	0.0
11	0.1786109388309123	0.0	0.0	0.0	0.0
12	0.1945460611014494	0.0	0.0	0.0	0.0
13	0.21128367154388406	0.0	0.0	0.0	0.0
14	0.2253845351357982	0.0	0.0	0.0	0.0
15	0.2441856865916837	0.0	0.0	0.0	0.0
16	0.2588597560206675	0.0	0.0	0.0	0.0
17	0.2755973664631022	0.0	0.0	0.0	0.0
18	0.320651345256779	0.0	0.0	0.0	0.0
19	0.3396817790474924	0.0	0.0	0.0	0.0
20	0.352865513300095	0.0	0.0	0.0	0.0
21	0.3717813059233945	0.0	0.0	0.0	0.0
22	0.383360063832202	0.0	0.0	0.0	0.0
23	0.39688772158704644	0.0	0.0	0.0	0.0
24	0.40754935015654253	0.0	0.0	0.0	0.0
25	0.4231405489248378	0.0	0.0	0.0	0.0
26	0.4364389243448544	0.0	0.0	0.0	0.0
27	0.4482469645884898	0.0	0.0	0.0	0.0
28	0.46074285183660885	0.0	0.0	0.0	0.0
29	0.4755315624330066	0.0	0.0	0.0	0.0
30	0.49100812003388794	0.0	0.0	0.0	0.0
31	0.5024722367752815	0.0	0.0	0.0	0.0
32	0.5153120475256424	0.0	0.0	0.0	0.0
33	0.5275786524389335	0.0	0.0	0.0	0.0
34	0.5381256398410156	0.0	0.0	0.0	0.0
35	0.5467237273970609	0.0	0.0	0.0	0.0
36	0.5564682266272454	0.0	0.0	0.0	0.0
37	0.5618563614957004	0.0	0.0	0.0	0.0
38	0.568046984536053	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGC	40	0.0	155.46594	44
ATTAGTC	20	1.5034984E-6	155.46594	44
AAATACC	10	0.005754115	155.46594	44
TCCGATG	20	1.5034984E-6	155.46594	44
CCAATGG	10	0.005754115	155.46594	44
ACGATGA	10	0.005754115	155.46594	44
TGATTGA	10	0.005754115	155.46594	44
CTTGTCA	35	7.2759576E-12	155.46593	44
CAGGTTA	25	2.4418114E-8	155.46593	44
GTGCATA	15	9.308504E-5	155.46593	44
TATGGGT	50	0.0	155.46593	44
GTTAGTA	35	7.2759576E-12	155.46593	44
GTAGGCA	70	0.0	155.46593	44
ACCTCGC	30	3.9835868E-10	155.46593	44
GAAAGTC	45	0.0	155.46593	44
AAGCCTA	25	2.4418114E-8	155.46593	44
ATGAGAC	65	0.0	155.46593	44
ACCAGCG	15	9.308504E-5	155.46593	44
AACTGGA	30	3.9835868E-10	155.46593	44
TAGTGTC	25	2.4418114E-8	155.46593	44
>>END_MODULE