##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840965.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4817027
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2397343838845	39.0	35.0	39.0	31.0	39.0
2	36.87461581593792	39.0	35.0	39.0	33.0	39.0
3	37.05811925073287	39.0	35.0	39.0	33.0	39.0
4	37.6719902130505	39.0	35.0	40.0	34.0	41.0
5	38.30638918984677	40.0	35.0	41.0	35.0	41.0
6	38.33443325104883	40.0	35.0	41.0	35.0	41.0
7	38.30126673568572	40.0	35.0	41.0	35.0	41.0
8	38.205344084639755	40.0	35.0	41.0	34.0	41.0
9	38.11413865855433	40.0	35.0	41.0	34.0	41.0
10	37.99954743870026	40.0	35.0	41.0	34.0	41.0
11	37.920637978570596	40.0	35.0	41.0	34.0	41.0
12	37.91359068570718	40.0	35.0	41.0	34.0	41.0
13	37.805323906218504	39.0	35.0	41.0	34.0	41.0
14	37.82488368863201	39.0	35.0	41.0	34.0	41.0
15	37.792228276901916	39.0	35.0	41.0	34.0	41.0
16	37.750880989456775	39.0	35.0	41.0	34.0	41.0
17	37.76444184348562	39.0	35.0	41.0	34.0	41.0
18	37.802530066781856	39.0	35.0	41.0	34.0	41.0
19	37.86409168974971	40.0	35.0	41.0	34.0	41.0
20	37.89832213936106	40.0	35.0	41.0	34.0	41.0
21	37.83152533710108	40.0	35.0	41.0	34.0	41.0
22	37.76681654472769	40.0	35.0	41.0	34.0	41.0
23	37.65441962438658	39.0	35.0	41.0	33.0	41.0
24	37.54233098548129	39.0	35.0	41.0	33.0	41.0
25	37.46780953614358	39.0	35.0	41.0	33.0	41.0
26	37.399598281385344	39.0	35.0	41.0	33.0	41.0
27	37.35530733565072	39.0	35.0	41.0	33.0	41.0
28	37.285987693325474	38.0	35.0	41.0	33.0	41.0
29	37.1589331628582	38.0	35.0	41.0	33.0	41.0
30	37.06118583296783	38.0	35.0	41.0	33.0	41.0
31	36.95077391711322	38.0	35.0	41.0	33.0	41.0
32	36.84169215399016	37.0	35.0	41.0	33.0	41.0
33	36.749669743079494	37.0	35.0	40.0	33.0	41.0
34	36.71190169259213	37.0	35.0	40.0	33.0	41.0
35	36.56779591112688	36.0	35.0	40.0	33.0	41.0
36	36.529415250708524	36.0	35.0	40.0	33.0	41.0
37	36.49069646460283	36.0	35.0	40.0	33.0	41.0
38	36.41569249634137	36.0	35.0	40.0	33.0	41.0
39	36.33311313652904	35.0	35.0	40.0	33.0	41.0
40	36.230990225426474	35.0	35.0	40.0	33.0	41.0
41	36.14752555433533	35.0	35.0	40.0	33.0	41.0
42	36.08155293662488	35.0	35.0	40.0	33.0	41.0
43	35.96901728232082	35.0	35.0	39.0	32.0	41.0
44	35.79435086076106	35.0	35.0	39.0	32.0	41.0
45	35.6460696249299	35.0	34.0	39.0	31.0	41.0
46	35.38041656758776	35.0	34.0	39.0	31.0	41.0
47	35.25992781587884	35.0	34.0	39.0	31.0	41.0
48	35.281434889975486	35.0	34.0	39.0	31.0	41.0
49	35.306636854627016	35.0	34.0	39.0	31.0	41.0
50	35.5060072667514	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	22.0
21	69.0
22	231.0
23	545.0
24	1264.0
25	2629.0
26	4898.0
27	9561.0
28	17388.0
29	31186.0
30	54629.0
31	95351.0
32	159215.0
33	295764.0
34	727415.0
35	283973.0
36	366734.0
37	550435.0
38	654336.0
39	785707.0
40	775668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.39697493910663	19.561173312916868	37.0418517479765
2	27.21533427153304	20.122889076602643	29.082232671728846	23.579543980135465
3	24.142443046302212	18.835186101302735	27.28382049758077	29.738550354814286
4	20.95680593029684	20.028287157202982	31.428596933336685	27.5863099791635
5	21.771520068291082	21.036107956214487	30.59727504122356	26.595096934270867
6	25.844800122565225	22.859016567687913	25.907432115286046	25.388751194460816
7	29.9567554842437	20.741548677223523	21.537039339825164	27.76465649870761
8	26.82388950695107	20.88721944053874	24.216721226598896	28.072169825911292
9	26.568213132290936	22.27249297128706	24.808600823703085	26.35069307271892
10	28.552299997488078	19.24608269789644	23.903623542072737	28.297993762542745
11	29.78501054696185	21.56778444463774	22.999559686918925	25.647645321481487
12	28.420060755316506	20.63247725204779	23.81072391747026	27.13673807516545
13	27.040433860968598	20.3479449046061	24.949891291869445	27.66172994255586
14	25.50789107057112	22.035292723084176	21.940939919996296	30.515876286348405
15	25.36101624508229	23.19231343316116	23.526710562344782	27.91995975941177
16	28.06880675570222	23.644833213515305	21.399589414798793	26.886770615983675
17	27.533933274611083	24.326602279787927	19.523286873833175	28.616177571767814
18	26.272408271741053	21.817710384434218	20.5863907343679	31.323490609456826
19	27.41298730524035	22.237575168252118	22.550797410934173	27.79864011557336
20	29.165375240786485	20.46546552468981	22.841038673854225	27.52812056066948
21	29.5843058384352	25.191596393377075	19.36179722471973	25.862300543467992
22	28.901581826300742	24.233951771497235	20.89535724005699	25.96910916214503
23	30.895321948579486	23.827767625134758	20.3456820981074	24.931228328178356
24	30.75828721740609	22.815545771281748	20.85624597910703	25.569921032205134
25	28.907217227013188	24.552629682508016	21.776906502846554	24.76324658763224
26	29.915581540519266	24.82014448931789	21.72359675734986	23.540677212812987
27	27.837916246881278	26.015370738628874	20.67878110148169	25.46793191300816
28	29.16896020898447	23.112397002633507	22.199150152455186	25.519492635926838
29	30.8499078180448	24.459990302023314	21.854245466281192	22.83585641365069
30	29.42478232334845	25.965313858634104	20.204270513516594	24.405633304500853
31	27.342781989427117	24.039279962385642	23.0628530800426	25.55508496814464
32	27.1326820835906	22.251612422296535	24.634652719325185	25.981052774787685
33	26.233096024238765	23.16320111305286	24.378029818482958	26.225673044225413
34	28.042908675829274	23.42064032472383	23.769052954929585	24.76739804451731
35	25.66497405649918	23.617832276375893	23.144285777639805	27.572907889485123
36	27.453209162268617	23.129998424877225	24.37178333529017	25.04500907756399
37	25.337930855405034	25.24720560405826	22.261465115435737	27.153398425100967
38	26.848252705536847	24.915112248099565	21.624690575535134	26.61194447082846
39	26.014711955621735	26.223898919699074	21.034503615916968	26.726885508762226
40	25.884796510819584	25.55046078569465	21.994180748698692	26.57056195478707
41	27.07845906347906	23.833205393314554	21.604179598403014	27.48415594480337
42	26.313234433888915	25.75572182772689	22.06534193983103	25.865701798553157
43	26.919271537362327	23.99736908937489	23.268087217964155	25.81527215529863
44	26.057416549031988	24.016435978515542	22.296561660352882	27.629585812099588
45	27.91136028733763	22.67805571091331	24.928793314307985	24.48179068744108
46	25.472532513124147	25.9295102242396	23.683568472787186	24.914388789849067
47	28.160771119153782	22.708907251301845	21.557666822062576	27.572654807481793
48	25.245547638950583	24.060251577463873	21.329870177494374	29.364330606091166
49	25.463880153572564	24.433324080446877	20.98478597313114	29.11800979284942
50	22.83082335117924	29.147504782446816	22.28247891229827	25.739192954075673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	91779.0
1	69672.0
2	43366.0
3	33585.5
4	23445.5
5	15914.0
6	10906.0
7	9022.5
8	9217.5
9	10600.0
10	12376.5
11	12565.0
12	11990.5
13	10823.5
14	9434.5
15	8969.5
16	9132.5
17	9852.0
18	11423.0
19	13115.0
20	15178.5
21	17646.0
22	19999.5
23	23577.0
24	27111.0
25	30133.0
26	34200.5
27	38899.0
28	41051.5
29	41856.0
30	45016.0
31	51185.0
32	63177.0
33	75511.0
34	83255.5
35	87953.5
36	92866.5
37	95522.5
38	95410.0
39	105847.5
40	125720.0
41	146087.5
42	165978.0
43	192543.5
44	202893.0
45	184858.5
46	184750.0
47	204020.5
48	223005.0
49	245735.0
50	273670.5
51	291767.5
52	296236.0
53	298658.5
54	296098.5
55	293823.5
56	303452.0
57	316762.5
58	321539.5
59	319967.5
60	319990.0
61	319949.5
62	311705.5
63	299006.0
64	282401.5
65	267518.0
66	249104.0
67	227229.0
68	214809.5
69	207082.0
70	194377.0
71	182989.0
72	174071.5
73	158514.5
74	138781.5
75	123174.0
76	109967.5
77	89425.5
78	70714.0
79	59372.5
80	46936.5
81	34069.0
82	26138.0
83	21733.5
84	18513.5
85	14742.5
86	9447.0
87	5602.0
88	3434.0
89	2376.0
90	1712.0
91	1305.5
92	1125.0
93	895.0
94	701.5
95	399.5
96	275.0
97	275.0
98	139.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	29662.0
25	26321.0
26	21519.0
27	23385.0
28	26502.0
29	31697.0
30	27794.0
31	28840.0
32	34408.0
33	28889.0
34	28210.0
35	21261.0
36	21205.0
37	24408.0
38	28693.0
39	26727.0
40	31298.0
41	34512.0
42	49411.0
43	84886.0
44	212702.0
45	421132.0
46	552628.0
47	601199.0
48	821852.0
49	798996.0
50	778890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.12079488892838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47901881824	12.812649603421258
2	9.20242874548165	2.967009325717763
3	2.839173426700183	1.373091973977887
4	1.3348383470465668	0.860746208104556
5	0.7798393953617575	0.6285815469466408
6	0.5409260131373541	0.5232094384723839
7	0.36984526353143854	0.41735397438223887
8	0.2736482213028363	0.35291414778744845
9	0.22658926364966248	0.3287519138996572
>10	2.3009467936972188	8.800800654601703
>50	1.2188126809819595	14.409717756314327
>100	1.3454406218168615	37.999220613725896
>500	0.054163457264900745	6.044056963131271
>1k	0.03229464353353237	9.794659945549675
>5k	0.0015257311905605845	1.781160302821851
>10k+	5.085770635201948E-4	0.9060756311454862
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11487	0.23846658945444982	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	10616	0.2203848971575206	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	9061	0.1881035750889501	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	8924	0.1852594971960921	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7872	0.1634203005297666	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6699	0.13906918105296068	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	5542	0.11505021665853234	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	5352	0.11110587505529863	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4935	0.1024490832208331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02370756900469937	0.0	0.0	0.0	0.0
2	0.043844470873839816	0.0	0.0	0.0	0.0
3	0.05630028646299886	0.0	0.0	0.0	0.0
4	0.06815407096534855	0.0	0.0	0.0	0.0
5	0.08135723548985713	0.0	0.0	0.0	0.0
6	0.10193009090461815	0.0	0.0	0.0	0.0
7	0.11444818557172297	0.0	0.0	0.0	0.0
8	0.1285855362654185	0.0	0.0	0.0	0.0
9	0.1412697084737121	0.0	0.0	0.0	0.0
10	0.15252146188925245	0.0	0.0	0.0	0.0
11	0.1647281611666283	0.0	0.0	0.0	0.0
12	0.17593839519687143	0.0	0.0	0.0	0.0
13	0.18741850523154635	0.0	0.0	0.0	0.0
14	0.1996044448162736	0.0	0.0	0.0	0.0
15	0.2126207721069448	0.0	0.0	0.0	0.0
16	0.22362340921070195	0.0	0.0	0.0	0.0
17	0.2386534266882872	0.0	0.0	0.0	0.0
18	0.25746170822791736	0.0	0.0	0.0	0.0
19	0.26927397334496983	0.0	0.0	0.0	0.0
20	0.28021433137078117	0.0	0.0	0.0	0.0
21	0.2938949688262075	0.0	0.0	0.0	0.0
22	0.3060393890256376	0.0	0.0	0.0	0.0
23	0.31992762340754993	0.0	0.0	0.0	0.0
24	0.3312831752863333	0.0	0.0	0.0	0.0
25	0.34450709950349045	0.0	0.0	0.0	0.0
26	0.3608242179252888	0.0	0.0	0.0	0.0
27	0.37257420396439545	0.0	0.0	0.0	0.0
28	0.38675307404338816	0.0	0.0	0.0	0.0
29	0.4007035875032463	0.0	0.0	0.0	0.0
30	0.4174358997780166	0.0	0.0	0.0	0.0
31	0.43381529727776075	0.0	0.0	0.0	0.0
32	0.4478696092008619	0.0	0.0	0.0	0.0
33	0.4641244485447144	0.0	0.0	0.0	0.0
34	0.4768916595236024	0.0	0.0	0.0	0.0
35	0.48851708740681754	0.0	0.0	0.0	0.0
36	0.49860629803403633	0.0	0.0	0.0	0.0
37	0.5062458649287206	0.0	0.0	0.0	0.0
38	0.512369974260057	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	3110	0.0	179.24721	44
CTATCAT	2150	0.0	168.79507	44
AATGCAC	30	1.0773001E-6	166.28152	44
ACGCACC	95	0.0	131.27489	44
CGCTATC	30	2.1336746E-9	122.25341	43
CCGACGA	45	1.8189894E-12	108.6697	43
GCTCGAC	35	6.2354957E-9	104.78863	43
TTAAGCC	2655	0.0	104.29528	43
ACGACAC	230	0.0	103.02224	44
CCGATTG	110	0.0	102.036385	44
ACTTCTA	765	0.0	97.81265	44
CCCGCAT	105	9.094947E-12	95.01801	44
TAACTTC	185	0.0	87.63486	44
AACGACA	535	0.0	86.83419	43
CGATTGC	130	0.0	86.338486	44
CCTATGG	75	1.0214935E-6	83.140755	44
GCGATAC	30	3.730812E-5	81.50227	43
ATGACCG	40	4.1836756E-11	79.9935	42
GTGTTAG	1240	0.0	78.87316	43
AGTTTCG	130	0.0	75.232864	43
>>END_MODULE