FastQCFastQC Report
Tue 24 May 2016
ERR840964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6790252
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160790.23679533543085No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91470.13470781349499253No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA85840.1264165159113388No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83300.12267585945263887No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA74960.11039354651344309No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70870.10437020599530031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTT2050.0190.0567644
CTATCAT37600.0145.6979444
CGGTTAT152.6383443E-4119.7155543
TCGTACA401.5279511E-10104.7511143
AATCGCA3400.0104.175544
TCATACG1200.098.3879844
GTTAAGC1204.7293724E-1178.7103844
TTGACGC2250.078.7103744
ACTTCTA13750.074.703344
CGTATAT1250.074.1160642
AGTAACG550.073.2372142
CCTATCA40450.070.14236543
GTCGGTA1107.221388E-1064.399444
CCCTATC47650.064.076842
ATAAGAT8700.058.4817343
TGCATAC2300.056.0423942
AATGTCG251.19873785E-554.9172940
TCGCAAA10500.054.7271143
ACCTTAG15200.054.59063342
TAATGCG1301.8189894E-1254.49180244
Produced by FastQC (version 0.11.5)