##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840964.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6790252
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1000317808529	39.0	35.0	39.0	31.0	39.0
2	36.91068063453315	39.0	35.0	39.0	33.0	39.0
3	36.93904659208524	39.0	35.0	39.0	33.0	39.0
4	37.56831940846967	39.0	35.0	40.0	34.0	41.0
5	38.24826781097373	40.0	35.0	41.0	34.0	41.0
6	38.27155104111011	40.0	36.0	41.0	34.0	41.0
7	38.22292324349671	40.0	35.0	41.0	34.0	41.0
8	38.146888804715935	40.0	35.0	41.0	34.0	41.0
9	38.106780131282314	40.0	35.0	41.0	34.0	41.0
10	38.04440556845313	40.0	35.0	41.0	34.0	41.0
11	37.93612298924988	40.0	35.0	41.0	34.0	41.0
12	37.836746559626945	40.0	35.0	41.0	33.0	41.0
13	37.70328361892902	39.0	35.0	41.0	33.0	41.0
14	37.65760077829218	39.0	35.0	41.0	33.0	41.0
15	37.66741558339808	39.0	35.0	41.0	33.0	41.0
16	37.567914121596665	39.0	35.0	41.0	33.0	41.0
17	37.62693652606708	39.0	35.0	41.0	33.0	41.0
18	37.67372227127947	39.0	35.0	41.0	33.0	41.0
19	37.78655136804937	39.0	35.0	41.0	33.0	41.0
20	37.80595300439512	39.0	35.0	41.0	34.0	41.0
21	37.70910564144011	39.0	35.0	41.0	33.0	41.0
22	37.60093160018214	39.0	35.0	41.0	33.0	41.0
23	37.49296226413983	39.0	35.0	41.0	33.0	41.0
24	37.35900817819427	39.0	35.0	41.0	33.0	41.0
25	37.272263683862676	39.0	35.0	41.0	33.0	41.0
26	37.17414007537576	38.0	35.0	41.0	33.0	41.0
27	37.11882329207072	38.0	35.0	41.0	33.0	41.0
28	37.00275550928231	38.0	35.0	41.0	33.0	41.0
29	36.87465224778221	38.0	35.0	41.0	33.0	41.0
30	36.74433309454425	37.0	35.0	40.0	33.0	41.0
31	36.58283427503565	37.0	35.0	40.0	33.0	41.0
32	36.46177909782407	36.0	35.0	40.0	32.0	41.0
33	36.38671511548315	36.0	35.0	40.0	32.0	41.0
34	36.303330730198304	36.0	35.0	40.0	32.0	41.0
35	36.17764272702658	35.0	35.0	40.0	32.0	41.0
36	36.079628816442636	35.0	35.0	40.0	32.0	41.0
37	36.037180992932164	35.0	35.0	40.0	32.0	41.0
38	36.00136769222517	35.0	35.0	40.0	32.0	41.0
39	35.918956303562865	35.0	35.0	40.0	32.0	41.0
40	35.82958513650872	35.0	35.0	39.0	32.0	41.0
41	35.76551309151044	35.0	35.0	39.0	32.0	41.0
42	35.667931649360746	35.0	34.0	39.0	32.0	41.0
43	35.57073052601154	35.0	34.0	39.0	32.0	41.0
44	35.40741520533854	35.0	34.0	39.0	31.0	41.0
45	35.248282973346114	35.0	34.0	39.0	31.0	41.0
46	35.02723990777198	35.0	34.0	38.0	30.0	41.0
47	34.83775343129103	35.0	34.0	38.0	30.0	40.0
48	34.87610637987483	35.0	34.0	38.0	30.0	40.0
49	34.90546755260303	35.0	33.0	37.0	30.0	40.0
50	35.320244450196945	35.0	34.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	37.0
21	104.0
22	312.0
23	793.0
24	1912.0
25	4154.0
26	7785.0
27	14823.0
28	28396.0
29	51457.0
30	90104.0
31	155126.0
32	256778.0
33	459237.0
34	880267.0
35	443208.0
36	680732.0
37	940050.0
38	937449.0
39	996532.0
40	840992.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.02450689606218	18.634448323861914	35.34104478007591
2	30.339846002769853	18.475676602282213	30.05128528366841	21.133192111279524
3	25.665468674800284	18.986997831597414	29.119802917476406	26.227730576125897
4	22.81162760969696	19.545813616342958	30.64186719432504	27.00069157963504
5	21.89681620063585	21.35484809694839	30.37464588943091	26.373689812984853
6	25.644865610289575	24.30825836802522	25.859909175683022	24.186966846002182
7	29.155825144633805	20.833291606850526	23.979051145671765	26.0318321028439
8	29.05881843560445	20.6183511304146	24.565642040972854	25.757188393008096
9	29.759558260871614	21.509938070045116	23.95025987253492	24.780243796548344
10	30.034143062731694	18.71933471688532	23.545120269468644	27.701401950914338
11	29.98776775884017	20.18169576033408	25.035801322248425	24.794735158577325
12	31.097947469401728	20.5418296699445	23.281536532075688	25.078686328578087
13	27.528934124977983	20.27530053376517	24.348580877410736	27.847184463846116
14	24.136718342706573	22.659836483240976	25.330959734631353	27.8724854394211
15	28.60898240595489	23.919112280368974	21.76586377059349	25.70604154308264
16	26.10009171971821	24.881653876763338	22.599131814253727	26.419122589264727
17	29.871969405553727	25.36842520719408	19.518171048732803	25.241434338519397
18	28.639320013454583	22.372910460465974	20.60994201687949	28.377827509199953
19	28.47906086548776	22.345238438867952	21.53067367750122	27.64502701814307
20	28.58737790585681	22.13047468635921	21.93601945848254	27.34612794930144
21	28.198246545194493	27.338764452335496	19.625840101368844	24.837148901101166
22	29.561303468560517	25.319531587340204	20.762395858062412	24.356769086036866
23	28.400934162679086	26.01879871321418	21.231568430744545	24.348698693362188
24	29.343270323398897	26.419034227301136	21.985752516990534	22.251942932309436
25	28.455019203559512	27.442708057361475	20.637091834379635	23.465180904699377
26	28.683618347049872	28.125602967240994	21.4515508541451	21.73922783156403
27	28.56961016769028	27.591471926633925	20.234525620064954	23.604392285610842
28	27.947712509085132	26.65744993023282	20.698840394247235	24.695997166434815
29	28.314194489629653	27.769961319165652	21.10813038987449	22.807713801330205
30	29.4095677407687	26.97404155664366	21.04594205963076	22.57044864295688
31	27.03082547194168	27.294277079509765	21.900563202567806	23.774334245980754
32	26.085554832065327	27.30195489818837	23.25235330502667	23.360136964719633
33	25.34175581327127	25.306944634580407	23.13901394402832	26.212285608120002
34	25.99558875849383	27.363590888102202	23.02771518758973	23.613105165814236
35	25.446683378693947	26.363487864500375	21.921045205101574	26.268783551704107
36	26.226172569746836	27.175943590384882	23.35776590086484	23.24011793900344
37	24.91939274095563	29.230622491025677	21.822137424961983	24.02784734305671
38	26.608144347922753	27.055901502824753	21.511303149791956	24.82465099946054
39	26.151003896669252	27.92413133515031	21.42081822703591	24.504046541144533
40	24.350424904611824	27.849924905516872	22.535552428652835	25.26409776121847
41	25.533091633348352	26.76424020542893	22.332606926832135	25.37006123439058
42	25.04201382026282	27.552198137930723	21.967716957691476	25.438071084114977
43	24.642458847210733	27.478333553820228	21.996191548825053	25.883016050143986
44	25.217630093683486	27.822040335478544	21.887690976607956	25.072638594230018
45	26.49245669550751	25.986809114397758	22.805907230596592	24.714826959498136
46	25.211620763203985	27.55324342541193	22.13073134473116	25.10440446665292
47	25.580233376166056	26.992341374316688	22.477335921748733	24.95008932776853
48	24.887060168411285	27.376609198288627	20.510925688101917	27.22540494519817
49	24.464199031012658	27.606990316788423	21.29436066065946	26.634449991539462
50	23.102607515409318	30.0549033463231	23.948108554357635	22.894380583909943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	133838.0
1	102175.5
2	73778.0
3	70083.0
4	56030.5
5	37761.5
6	21855.5
7	17572.0
8	19256.0
9	21891.5
10	23593.0
11	23845.0
12	23223.5
13	21333.0
14	20144.0
15	22062.0
16	25102.0
17	27631.0
18	31251.5
19	35700.5
20	39861.5
21	43711.0
22	46983.0
23	57687.5
24	71423.5
25	80462.5
26	95516.5
27	115674.0
28	125877.5
29	122665.0
30	116274.5
31	114362.5
32	116842.5
33	123452.5
34	137726.0
35	145794.0
36	145743.0
37	153111.5
38	163990.0
39	172707.5
40	180112.0
41	201099.5
42	226042.5
43	226145.0
44	219638.0
45	220492.5
46	230950.5
47	252682.5
48	272249.5
49	296625.5
50	336815.5
51	363374.5
52	371375.0
53	370408.5
54	372052.5
55	382186.0
56	394460.5
57	410813.0
58	417942.5
59	418732.0
60	427183.5
61	438410.5
62	433706.0
63	420207.5
64	403043.0
65	384657.5
66	369502.0
67	360130.5
68	343540.0
69	313872.0
70	289663.0
71	273704.5
72	247135.0
73	213468.5
74	187408.0
75	162700.5
76	136561.0
77	105952.0
78	82919.0
79	69690.0
80	55015.0
81	39972.0
82	29502.0
83	23308.0
84	18291.5
85	14522.5
86	11214.5
87	8426.0
88	5181.5
89	2789.0
90	1800.0
91	1006.5
92	700.5
93	693.0
94	492.5
95	195.5
96	95.5
97	70.0
98	89.5
99	109.0
100	109.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	52699.0
25	41501.0
26	34008.0
27	38586.0
28	41904.0
29	45800.0
30	48035.0
31	45184.0
32	50910.0
33	50553.0
34	47800.0
35	39870.0
36	36220.0
37	46203.0
38	49084.0
39	44884.0
40	56048.0
41	55664.0
42	76816.0
43	112972.0
44	281566.0
45	586109.0
46	752913.0
47	809779.0
48	1093515.0
49	1108165.0
50	1143464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.911437598846163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51906290719782	14.470854832916554
2	10.340311957557814	3.911003286759148
3	3.518602318746296	1.9962548455837794
4	1.6496939302397589	1.2479233527569789
5	0.9693798837998732	0.9166183591029023
6	0.6772584112474498	0.7684758109559904
7	0.49954351819957177	0.6612960250637492
8	0.3790129894533473	0.5734144399359293
9	0.2950063634343497	0.5021094990016114
>10	2.9739507971592842	13.094509788463915
>50	1.2449940289003651	17.107545781954457
>100	0.865204236046751	27.674155855795778
>500	0.04383451396291586	5.506779032949282
>1k	0.021800052398669383	8.001945466017366
>5k	0.0021096824901938116	2.8543292522543386
>10k+	2.3440916557709014E-4	0.712784370488251
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16079	0.23679533543085	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9147	0.13470781349499253	No Hit
AGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	8584	0.1264165159113388	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8330	0.12267585945263887	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	7496	0.11039354651344309	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7087	0.10437020599530031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0343875308309618	0.0	0.0	0.0	0.0
2	0.053930251778579054	0.0	0.0	0.0	0.0
3	0.06663964754179963	0.0	0.0	0.0	0.0
4	0.07967303717152176	0.0	0.0	0.0	0.0
5	0.09286842373449468	0.0	0.0	0.0	0.0
6	0.12115897907765426	0.0	0.0	0.0	0.0
7	0.13323511410180358	0.0	0.0	0.0	0.0
8	0.1475202982157363	0.0	0.0	0.0	0.0
9	0.15962588722774942	0.0	0.0	0.0	0.0
10	0.16909534432595433	0.0	0.0	0.0	0.0
11	0.18130402229549064	0.0	0.0	0.0	0.0
12	0.19393978308905177	0.0	0.0	0.0	1.472699393188942E-5
13	0.20522066044087908	0.0	0.0	0.0	1.472699393188942E-5
14	0.21670771570775282	0.0	0.0	0.0	1.472699393188942E-5
15	0.22865130778651513	0.0	0.0	0.0	1.472699393188942E-5
16	0.24047708391382233	0.0	0.0	0.0	1.472699393188942E-5
17	0.25538080177289446	0.0	0.0	0.0	1.472699393188942E-5
18	0.27389263314527945	0.0	0.0	0.0	1.472699393188942E-5
19	0.29110848905165815	0.0	0.0	0.0	1.472699393188942E-5
20	0.30490768236583854	0.0	0.0	0.0	1.472699393188942E-5
21	0.3274988910573569	0.0	0.0	0.0	1.472699393188942E-5
22	0.34533328070887503	0.0	0.0	0.0	1.472699393188942E-5
23	0.3621662347730246	0.0	0.0	0.0	1.472699393188942E-5
24	0.38010371338206594	0.0	0.0	0.0	1.472699393188942E-5
25	0.3971575723551939	0.0	0.0	0.0	1.472699393188942E-5
26	0.4238576123537094	0.0	0.0	0.0	1.472699393188942E-5
27	0.43982167377587755	0.0	0.0	0.0	1.472699393188942E-5
28	0.4571406186397795	0.0	0.0	0.0	1.472699393188942E-5
29	0.47624152976944006	0.0	0.0	0.0	1.472699393188942E-5
30	0.4954160758687601	0.0	0.0	0.0	1.472699393188942E-5
31	0.514752618901331	0.0	0.0	0.0	1.472699393188942E-5
32	0.5339713459824466	0.0	0.0	0.0	1.472699393188942E-5
33	0.5503477632347077	0.0	0.0	0.0	1.472699393188942E-5
34	0.5641764105367518	0.0	0.0	0.0	1.472699393188942E-5
35	0.5762230915730373	0.0	0.0	0.0	1.472699393188942E-5
36	0.5883728615668461	0.0	0.0	0.0	1.472699393188942E-5
37	0.5955890885934719	0.0	0.0	0.0	1.472699393188942E-5
38	0.6015535211358871	0.0	0.0	0.0	1.472699393188942E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTT	205	0.0	190.05676	44
CTATCAT	3760	0.0	145.69794	44
CGGTTAT	15	2.6383443E-4	119.71555	43
TCGTACA	40	1.5279511E-10	104.75111	43
AATCGCA	340	0.0	104.1755	44
TCATACG	120	0.0	98.38798	44
GTTAAGC	120	4.7293724E-11	78.71038	44
TTGACGC	225	0.0	78.71037	44
ACTTCTA	1375	0.0	74.7033	44
CGTATAT	125	0.0	74.11606	42
AGTAACG	55	0.0	73.23721	42
CCTATCA	4045	0.0	70.142365	43
GTCGGTA	110	7.221388E-10	64.3994	44
CCCTATC	4765	0.0	64.0768	42
ATAAGAT	870	0.0	58.48173	43
TGCATAC	230	0.0	56.04239	42
AATGTCG	25	1.19873785E-5	54.91729	40
TCGCAAA	1050	0.0	54.72711	43
ACCTTAG	1520	0.0	54.590633	42
TAATGCG	130	1.8189894E-12	54.491802	44
>>END_MODULE