FastQCFastQC Report
Tue 24 May 2016
ERR840963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6170751
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA106700.17291250287039617No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG105420.17083820105526865No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA95890.15539437582232696No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG87580.14192761950692875No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64500.10452536490291053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA307.2759576E-12280.325844
ACGTATG207.9764504E-8280.3257844
CTATCAT49250.0196.9395444
TAAGCCA24800.0167.291244
ACGACAC1450.0164.3289244
TTGACGC700.0160.1861744
ATTGTAG1400.0120.13962644
TACGATC507.651965E-6112.1303144
ACTTCTA17800.0106.3033244
AACGACA5050.099.86484543
TGCATGA1850.090.9164744
CGTGTGC7200.089.54851544
ATATATC1002.8703653E-884.0977344
CATACAT1850.083.34009644
AACTACC11050.081.1803244
ATTAAGC23350.081.11484542
CTTTACA15550.079.3204844
CCTATCA56950.078.2417643
GGTAAGG1105.5588316E-876.45248444
ATAGCCA4400.076.45248444
Produced by FastQC (version 0.11.5)