##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840963.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6170751
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.797905959906664	39.0	37.0	39.0	32.0	39.0
2	37.55019461974726	39.0	37.0	39.0	35.0	39.0
3	37.67839862603434	39.0	37.0	39.0	35.0	39.0
4	38.2411526571077	39.0	38.0	40.0	35.0	41.0
5	38.74187971609938	40.0	38.0	41.0	35.0	41.0
6	38.69443946125844	40.0	38.0	41.0	35.0	41.0
7	38.643746279828825	40.0	38.0	41.0	35.0	41.0
8	38.4647627168881	40.0	38.0	41.0	34.0	41.0
9	38.280643960516315	40.0	37.0	41.0	34.0	41.0
10	38.090258543895224	40.0	37.0	41.0	34.0	41.0
11	37.87621133959222	39.0	36.0	41.0	33.0	41.0
12	37.802481253902485	39.0	36.0	41.0	33.0	41.0
13	37.63544096982685	39.0	36.0	40.0	33.0	41.0
14	37.4905106363877	39.0	35.0	40.0	33.0	41.0
15	37.44736791356514	39.0	35.0	40.0	33.0	41.0
16	37.2801804837045	38.0	35.0	40.0	33.0	41.0
17	37.31093476304586	38.0	35.0	40.0	33.0	41.0
18	37.30364796764608	38.0	35.0	40.0	33.0	41.0
19	37.347334546475786	38.0	35.0	40.0	33.0	41.0
20	37.249057529626455	38.0	35.0	40.0	33.0	41.0
21	37.17620367439879	38.0	35.0	40.0	33.0	41.0
22	36.89359544729645	38.0	35.0	40.0	33.0	41.0
23	36.79417075814597	37.0	35.0	40.0	33.0	41.0
24	36.52216513030586	37.0	35.0	40.0	32.0	41.0
25	36.42000059778262	37.0	35.0	40.0	32.0	41.0
26	36.2989234476479	36.0	35.0	40.0	32.0	41.0
27	36.169854890141956	36.0	35.0	40.0	32.0	41.0
28	36.056729614515	36.0	35.0	40.0	32.0	41.0
29	35.96131718720213	35.0	35.0	40.0	32.0	41.0
30	35.84794270853066	35.0	35.0	39.0	32.0	41.0
31	35.60414067638119	35.0	34.0	39.0	31.0	41.0
32	35.54164216378607	35.0	34.0	39.0	31.0	41.0
33	35.449750316213084	35.0	34.0	39.0	31.0	41.0
34	35.37341675746631	35.0	34.0	39.0	31.0	41.0
35	35.25552694291404	35.0	34.0	38.0	31.0	41.0
36	35.158240289749784	35.0	34.0	38.0	31.0	41.0
37	35.14614950320588	35.0	34.0	37.0	31.0	40.0
38	35.05538883237154	35.0	34.0	37.0	31.0	40.0
39	34.99668842666324	35.0	34.0	37.0	31.0	40.0
40	34.97346393413811	35.0	34.0	37.0	31.0	40.0
41	34.91836349782901	35.0	34.0	37.0	31.0	40.0
42	34.80083489396196	35.0	34.0	36.0	31.0	40.0
43	34.71404424313113	35.0	34.0	36.0	31.0	40.0
44	34.55267936799036	35.0	34.0	36.0	31.0	39.0
45	34.394197815111454	35.0	34.0	36.0	30.0	39.0
46	34.196648838373484	35.0	33.0	36.0	30.0	39.0
47	34.089080430868876	35.0	33.0	35.0	30.0	39.0
48	34.049697146960966	35.0	33.0	36.0	29.0	39.0
49	34.04763083802151	35.0	33.0	35.0	29.0	39.0
50	34.49909960179573	35.0	33.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	48.0
22	162.0
23	401.0
24	1128.0
25	2787.0
26	6050.0
27	12913.0
28	26358.0
29	50649.0
30	91535.0
31	155776.0
32	247978.0
33	402486.0
34	668412.0
35	764036.0
36	985345.0
37	946340.0
38	762453.0
39	716108.0
40	329763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.77701336514794	17.13088082795757	38.092105806894494
2	27.177000011830003	18.943334449891108	29.448036389736032	24.43162914854286
3	24.8703277769594	16.384018736131146	28.262329820146686	30.483323666762765
4	21.61471107811675	17.815740742091197	32.491409878635515	28.078138301156535
5	22.231572785873226	21.3548399538403	29.176383879368977	27.237203380917492
6	24.701174946128923	22.134015778630513	27.748372929000055	25.416436346240513
7	29.920685504892354	19.259876147976154	22.744508731595232	28.07492961553626
8	27.68769960090757	20.600166819241288	23.2154400655609	28.49669351429024
9	27.124461836168727	19.618892416822522	26.203471830252106	27.053173916756645
10	30.54552031025073	17.434555372595653	24.706960303535176	27.31296401361844
11	29.16085902672138	19.744258032774294	23.936324768249438	27.15855817225488
12	30.638782864516816	19.912065808521522	22.434984007619168	27.01416731934249
13	29.9679244876353	18.740863146155142	24.829198261281327	26.462014104928233
14	26.031871971499093	21.368922518507066	21.278560745685574	31.320644764308263
15	25.60048201588429	21.963242399506964	23.94340656429015	28.492869020318594
16	28.35215681203147	22.856926166685383	21.119633574584356	27.67128344669879
17	28.254243284164275	24.388425331049657	19.478714989472106	27.87861639531396
18	27.195539084302705	20.29996024795037	19.76056074860256	32.74393991914437
19	30.25109909636607	19.91323260329253	21.025560746171738	28.81010755416966
20	30.304074820066475	19.44008111816536	22.860264496169105	27.395579565599064
21	29.687796509695495	23.60335071047268	19.211794480120815	27.497058299711007
22	30.64485992061582	21.690730998544584	22.22142815355862	25.442980927280974
23	31.41692153839946	22.109237595229494	21.18032310815977	25.293517758211276
24	32.5640752640967	20.854155353213898	22.823267378638352	23.758502004051046
25	30.139938297727433	23.352443322123005	21.927450422833207	24.580167957316363
26	31.87288378332569	23.848866818116722	20.564616791510037	23.71363260704755
27	29.737392348032525	23.819990804233353	20.64302677751115	25.799590070222965
28	30.640697109061783	22.710493699237215	20.98588738067341	25.662921811027594
29	30.837179142091	23.698402379113286	20.835219270830983	24.62919920796473
30	31.674669185713167	23.7640212468775	19.455256194269673	25.10605337313966
31	28.617036050987227	23.688504484338853	21.768621680092515	25.925837784581407
32	27.527082784819974	22.641757978974432	23.803318710687975	26.027840525517625
33	27.31785226967648	21.612178147669432	23.579151524880505	27.49081805777358
34	28.09933515838874	21.96434658764135	24.805103183947878	25.131215070022034
35	26.085699347929058	20.769149470699293	22.9835958740212	30.161555307350447
36	27.457804480763333	22.02423596436106	25.607079001536952	24.910880553338654
37	25.815844119529423	24.775527469091113	23.93816906404053	25.470459347338938
38	27.041554174834985	22.93533487215107	22.567962158996902	27.455148794017042
39	27.537153191591546	23.81129917777581	22.046101679492345	26.6054459511403
40	26.290666821275455	23.34804116059097	23.170253172494153	27.191038845639426
41	27.45108102247752	22.07127194931932	22.02971989295076	28.447927135252403
42	25.48370862655378	24.161395829448317	22.511734516509456	27.84316102748844
43	27.790765431738258	21.619810967395217	23.249647667379286	27.339775933487235
44	26.730004042730016	23.72782845081746	22.667747527502208	26.87441997895032
45	27.156341833368362	21.546943615072664	23.96633314153406	27.330381410024906
46	25.77928239463474	24.107288717132867	23.720837671597035	26.392591216635353
47	27.84876259888078	21.896847313484646	23.4714916471381	26.782898440496478
48	25.155649022782296	24.321653134058643	20.19012250065569	30.332575342503375
49	25.895037061413817	23.708021876420165	20.991125110348467	29.405815951817548
50	22.603568851064225	28.315044835449633	24.35571378174163	24.725672531744515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68968.0
1	54250.0
2	43652.5
3	42666.5
4	31725.5
5	20297.0
6	12010.5
7	10971.0
8	14574.5
9	16313.5
10	15102.0
11	14077.0
12	13752.5
13	12694.0
14	11617.0
15	11436.5
16	11634.5
17	11678.0
18	12082.5
19	13038.5
20	14543.5
21	17331.0
22	20708.5
23	26358.5
24	31619.0
25	32894.5
26	34980.5
27	39650.5
28	43668.0
29	48061.0
30	54186.5
31	64024.5
32	79422.5
33	90749.0
34	99198.0
35	104506.0
36	105312.0
37	111060.5
38	115645.0
39	122807.0
40	143284.0
41	174867.0
42	201920.0
43	214690.5
44	217602.5
45	210145.5
46	217060.0
47	238391.0
48	264665.0
49	306950.5
50	348368.5
51	371679.0
52	387980.0
53	390957.5
54	383602.5
55	383354.0
56	388529.5
57	404430.0
58	415614.0
59	411183.0
60	413611.0
61	429776.0
62	434696.5
63	422247.0
64	397125.5
65	373020.0
66	363036.5
67	360548.0
68	353108.0
69	333203.0
70	294310.5
71	260426.5
72	244101.0
73	227366.5
74	211838.0
75	192256.5
76	159914.5
77	117700.0
78	87233.5
79	69597.5
80	53626.0
81	40295.0
82	29948.0
83	24609.0
84	22620.0
85	20721.5
86	17203.0
87	13609.0
88	8536.0
89	3924.0
90	1953.0
91	981.5
92	689.0
93	611.5
94	761.0
95	832.5
96	729.0
97	695.0
98	347.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	48124.0
25	33931.0
26	31561.0
27	35007.0
28	37041.0
29	46332.0
30	55948.0
31	45058.0
32	56837.0
33	43004.0
34	41902.0
35	38711.0
36	35449.0
37	44404.0
38	38685.0
39	36698.0
40	46252.0
41	45060.0
42	68494.0
43	103219.0
44	310270.0
45	552013.0
46	697952.0
47	765835.0
48	998574.0
49	1046996.0
50	867394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.025309273189144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41828436898233	15.70350397161765
2	9.912767498708984	3.970124698297299
3	3.1344907748004456	1.8830744153801153
4	1.4690113300315486	1.1766962483880272
5	0.82298229951666	0.8240237537090748
6	0.5887202724878334	0.7073583319179029
7	0.4052775094741899	0.5681065228081947
8	0.31404781383773295	0.5031123678935625
9	0.24789773412564148	0.44678059145899063
>10	2.5868814333049315	12.286288032529017
>50	1.1895446590646073	17.22591166199704
>100	0.8460578194069692	28.80435966090571
>500	0.04341867818264426	6.011454862214752
>1k	0.018677308491975464	6.870872527816167
>5k	0.0014553746876863997	1.9879832024003354
>10k+	4.8512489589546656E-4	1.0303491506662088
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	10670	0.17291250287039617	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG	10542	0.17083820105526865	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	9589	0.15539437582232696	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	8758	0.14192761950692875	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6450	0.10452536490291053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01427703046193243	0.0	0.0	0.0	0.0
2	0.029056430894716056	0.0	0.0	0.0	0.0
3	0.03779118619435463	0.0	0.0	0.0	0.0
4	0.04767653078207174	0.0	0.0	0.0	0.0
5	0.057480847955135445	0.0	0.0	0.0	0.0
6	0.0732325773637601	0.0	0.0	0.0	0.0
7	0.08399301802973415	0.0	0.0	0.0	0.0
8	0.09357045844176827	0.0	0.0	0.0	0.0
9	0.10343959754655471	0.0	0.0	0.0	0.0
10	0.11334114761720251	0.0	0.0	0.0	0.0
11	0.1222541632290786	0.0	0.0	0.0	0.0
12	0.1319612475045582	0.0	0.0	0.0	0.0
13	0.14131181115556274	0.0	0.0	0.0	0.0
14	0.15131059412379466	0.0	0.0	0.0	0.0
15	0.16143902095547202	0.0	0.0	0.0	0.0
16	0.17111369426509027	0.0	0.0	0.0	0.0
17	0.1826844090775985	0.0	0.0	0.0	0.0
18	0.20313572853612144	0.0	0.0	0.0	0.0
19	0.21410684048019438	0.0	0.0	0.0	0.0
20	0.22394356861911946	0.0	0.0	0.0	0.0
21	0.23512535184129127	0.0	0.0	0.0	0.0
22	0.24447591549229583	0.0	0.0	0.0	0.0
23	0.25562528774860627	0.0	0.0	0.0	0.0
24	0.26776319446368846	0.0	0.0	0.0	0.0
25	0.2796094024860183	0.0	0.0	0.0	0.0
26	0.29408089874311893	0.0	0.0	0.0	0.0
27	0.30558679162390445	0.0	0.0	0.0	0.0
28	0.3184539450708674	0.0	0.0	0.0	0.0
29	0.3310456053080087	0.0	0.0	0.0	0.0
30	0.3449661151454661	0.0	0.0	0.0	0.0
31	0.3583356385632802	0.0	0.0	0.0	0.0
32	0.3713972578054114	0.0	0.0	0.0	0.0
33	0.3840051235254834	0.0	0.0	0.0	0.0
34	0.39461971484508124	0.0	0.0	0.0	0.0
35	0.40448885394986767	0.0	0.0	0.0	0.0
36	0.41361254083984267	0.0	0.0	0.0	0.0
37	0.42072674784641284	0.0	0.0	0.0	0.0
38	0.4263014339745681	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATA	30	7.2759576E-12	280.3258	44
ACGTATG	20	7.9764504E-8	280.32578	44
CTATCAT	4925	0.0	196.93954	44
TAAGCCA	2480	0.0	167.2912	44
ACGACAC	145	0.0	164.32892	44
TTGACGC	70	0.0	160.18617	44
ATTGTAG	140	0.0	120.139626	44
TACGATC	50	7.651965E-6	112.13031	44
ACTTCTA	1780	0.0	106.30332	44
AACGACA	505	0.0	99.864845	43
TGCATGA	185	0.0	90.91647	44
CGTGTGC	720	0.0	89.548515	44
ATATATC	100	2.8703653E-8	84.09773	44
CATACAT	185	0.0	83.340096	44
AACTACC	1105	0.0	81.18032	44
ATTAAGC	2335	0.0	81.114845	42
CTTTACA	1555	0.0	79.32048	44
CCTATCA	5695	0.0	78.24176	43
GGTAAGG	110	5.5588316E-8	76.452484	44
ATAGCCA	440	0.0	76.452484	44
>>END_MODULE