##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840962.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7024653
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.41679524953047	39.0	37.0	39.0	35.0	39.0
2	37.84045574920213	39.0	38.0	39.0	35.0	39.0
3	37.97234340258515	39.0	38.0	39.0	35.0	39.0
4	38.53129969551521	39.0	38.0	41.0	35.0	41.0
5	39.23740090791673	40.0	39.0	41.0	35.0	41.0
6	39.178759577163454	40.0	38.0	41.0	35.0	41.0
7	39.21362734927975	40.0	38.0	41.0	35.0	41.0
8	39.12406548764758	40.0	38.0	41.0	35.0	41.0
9	39.02355475779373	40.0	38.0	41.0	35.0	41.0
10	38.93982549743026	40.0	38.0	41.0	35.0	41.0
11	38.76620467943399	40.0	38.0	41.0	35.0	41.0
12	38.79152806551441	40.0	38.0	41.0	35.0	41.0
13	38.69387569748997	40.0	38.0	41.0	35.0	41.0
14	38.66029738408431	40.0	38.0	41.0	35.0	41.0
15	38.67793768603232	40.0	38.0	41.0	35.0	41.0
16	38.59409397161682	40.0	38.0	41.0	35.0	41.0
17	38.63464572556111	40.0	38.0	41.0	35.0	41.0
18	38.66194686057803	40.0	38.0	41.0	35.0	41.0
19	38.77339535490223	40.0	38.0	41.0	35.0	41.0
20	38.761427646319326	40.0	38.0	41.0	35.0	41.0
21	38.74034034136633	40.0	38.0	41.0	35.0	41.0
22	38.66109315292869	40.0	38.0	41.0	35.0	41.0
23	38.562508781572554	40.0	37.0	41.0	35.0	41.0
24	38.36576312025662	40.0	37.0	41.0	34.0	41.0
25	38.32542730469741	40.0	37.0	41.0	34.0	41.0
26	38.27854290331435	40.0	37.0	41.0	34.0	41.0
27	38.18903786648818	40.0	36.0	41.0	34.0	41.0
28	38.106392663186725	40.0	36.0	41.0	34.0	41.0
29	38.00655180479818	40.0	35.0	41.0	33.0	41.0
30	37.922876901806205	40.0	35.0	41.0	33.0	41.0
31	37.7727706699484	40.0	35.0	41.0	33.0	41.0
32	37.665074277467376	39.0	35.0	41.0	33.0	41.0
33	37.557449620602384	39.0	35.0	41.0	33.0	41.0
34	37.490964114780766	39.0	35.0	41.0	33.0	41.0
35	37.32462612415732	39.0	35.0	41.0	33.0	41.0
36	37.27100085278451	39.0	35.0	41.0	33.0	41.0
37	37.18279865284941	38.0	35.0	41.0	33.0	41.0
38	37.15088693887173	38.0	35.0	41.0	33.0	41.0
39	37.05170609926529	38.0	35.0	41.0	33.0	41.0
40	36.997985795116634	38.0	35.0	41.0	33.0	41.0
41	36.911173098386904	37.0	35.0	40.0	33.0	41.0
42	36.793877538982194	37.0	35.0	40.0	33.0	41.0
43	36.699930677921564	37.0	35.0	40.0	33.0	41.0
44	36.55624498861971	37.0	35.0	40.0	32.0	41.0
45	36.36270798473699	36.0	35.0	40.0	32.0	41.0
46	36.12188881557305	36.0	35.0	40.0	31.0	41.0
47	35.93450273420502	36.0	34.0	40.0	31.0	41.0
48	35.89915666788566	36.0	34.0	40.0	31.0	41.0
49	35.9888489077114	36.0	34.0	40.0	31.0	41.0
50	36.21512742595519	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	18.0
21	83.0
22	148.0
23	343.0
24	665.0
25	1306.0
26	2474.0
27	5228.0
28	11172.0
29	22321.0
30	43144.0
31	79438.0
32	135109.0
33	237888.0
34	458067.0
35	395123.0
36	628510.0
37	947461.0
38	1103790.0
39	1427159.0
40	1525204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.255368343461235	18.081661827281717	39.662969829257044
2	25.538670735764455	20.546281787869095	32.52014014073008	21.394907335636365
3	24.681845494716963	17.526032958496312	28.399481084688453	29.392640462098267
4	21.95043655537149	20.30733760087509	33.47910565831508	24.263120185438343
5	22.197623142381552	20.24387539142503	30.07277370141984	27.485727764773575
6	22.535219889153243	24.180027113083025	27.864721574147506	25.42003142361623
7	29.727034203682372	19.92385958423854	21.90029884750179	28.448807364577295
8	25.305933261045066	20.862468224409092	25.40163905605017	28.429959458495674
9	26.686570852681264	21.268466926409037	26.479201179047564	25.56576104186214
10	28.16890741791801	19.488991128814476	23.94783059035087	28.394270862916642
11	26.872345153561323	21.596952902869365	24.617457972657157	26.91324397091216
12	28.084789383902663	22.79060616944353	23.164418228202873	25.960186218450932
13	28.272713257153057	20.86253940230215	25.235239377660363	25.62950796288443
14	24.35276162395495	22.197950560689616	23.2776053137429	30.171682501612533
15	26.069942529545585	23.373595820320233	24.059380584350574	26.497081065783608
16	26.377616090075907	24.217295857887926	21.23548309076619	28.16960496126997
17	26.23459123176618	26.14670076941879	20.80125523637965	26.817452762435384
18	26.2793051841849	22.399383998042325	20.28997019496906	31.031340622803715
19	28.53335246595099	21.49119678936454	22.350840674977114	27.624610069707355
20	27.669694147169977	21.16404895729369	24.89848253002675	26.267774365509585
21	28.15497078645735	26.463855225304368	20.144767293131775	25.236406695106506
22	29.476274486440822	23.427719490201156	21.524835461623514	25.571170561734508
23	31.21320013956561	22.369033744442607	21.657183635974615	24.760582480017163
24	28.373287620043296	23.06000025908753	23.357851270375917	25.208860850493252
25	27.658089155978168	24.737264071629596	21.418910908957727	26.185735863434513
26	29.026918723653505	25.22465170933859	22.562116667735356	23.186312899272547
27	27.17025328475775	26.85694772875185	20.94309546584153	25.029703520648866
28	27.215022638515947	23.33172642405972	21.996246667117475	27.45700427030686
29	28.108594903583278	25.751304805485297	22.120992383152295	24.019107907779123
30	27.469409420194975	26.436919424823245	21.242045066359648	24.85162608862213
31	26.578124134999186	25.626034855517666	22.781357102827894	25.014483906655254
32	25.92721089864849	24.53404545991732	25.2403896666534	24.298353974780788
33	25.38916893311069	22.8117816204268	23.94794504732624	27.85110439913628
34	27.63524015665157	24.01857070802073	24.275000715037937	24.071188420289765
35	24.81460890937919	22.6806073451752	22.866470866853078	29.638312878592533
36	24.52441865560345	23.798571256534924	26.786166811759536	24.890843276102085
37	24.21845200872251	26.535543639262254	25.39504524405055	23.850959107964687
38	25.255877110171493	24.040893840583024	23.393484493158194	27.30974455608729
39	26.101764292318347	25.76472660184549	23.079500555255787	25.05400855058038
40	26.052145875132478	24.81201957463181	23.599557992184835	25.536276558050876
41	26.220554396523188	23.696341638352482	22.392505349288918	27.690598615835416
42	23.794655532851667	27.099598998364776	22.76977614980081	26.335969318982748
43	26.035712155384633	23.804282814734325	23.039805382295143	27.120199647585896
44	25.29657133543712	24.52154883285516	22.951093842523697	27.23078598918402
45	26.067397494446908	22.999681062762697	25.729262259911373	25.203659182879022
46	24.94008117553608	26.43399224618126	23.036223331681498	25.589703246601157
47	26.364235561519106	22.838057009975635	24.98760647824923	25.810100950256025
48	23.647922399540583	24.44197555240687	21.547253712955612	30.36284833509694
49	25.125657248518095	25.784970099018118	21.00171678509704	28.087655867366745
50	21.37117620841943	29.151493051335038	25.967586402495847	23.509744337749687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90927.0
1	73770.0
2	63617.0
3	63457.0
4	48561.5
5	32432.5
6	19990.0
7	17497.5
8	19896.0
9	21600.5
10	21800.0
11	19525.5
12	17080.0
13	16330.5
14	18151.5
15	20441.0
16	20741.0
17	19942.5
18	20168.0
19	23246.5
20	27023.5
21	30701.5
22	33772.5
23	38849.5
24	45069.0
25	47736.0
26	50190.0
27	55411.5
28	60902.5
29	66889.5
30	74967.5
31	90163.5
32	114597.0
33	134086.0
34	153418.5
35	169124.5
36	170461.0
37	169180.5
38	171942.0
39	190395.0
40	223037.5
41	269036.0
42	306912.5
43	317526.0
44	312847.0
45	300451.5
46	303842.5
47	315317.5
48	332482.0
49	380369.5
50	441185.5
51	466521.5
52	453889.0
53	424137.5
54	410095.5
55	410136.0
56	408120.5
57	415903.0
58	420231.5
59	411924.5
60	405739.0
61	409666.5
62	411545.5
63	407647.0
64	385443.5
65	357684.5
66	338573.0
67	324723.0
68	317757.5
69	304263.0
70	265728.0
71	231745.5
72	222210.5
73	214579.5
74	195006.0
75	166509.0
76	137094.5
77	102223.5
78	79082.0
79	73778.0
80	64027.5
81	45455.0
82	32876.5
83	26652.0
84	20660.0
85	16277.0
86	12236.0
87	8528.0
88	5499.5
89	3649.5
90	2994.5
91	2573.5
92	2060.5
93	1816.0
94	1218.0
95	571.5
96	471.5
97	466.0
98	244.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	48542.0
25	36146.0
26	31693.0
27	31717.0
28	36455.0
29	42746.0
30	41580.0
31	45731.0
32	57919.0
33	44083.0
34	46237.0
35	34950.0
36	32851.0
37	41980.0
38	41464.0
39	37821.0
40	49935.0
41	50260.0
42	69613.0
43	98075.0
44	276122.0
45	594151.0
46	778625.0
47	860102.0
48	1161654.0
49	1200151.0
50	1234050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.510364490396622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75509303111939	16.152956635433537
2	9.90735528238186	4.064069359550166
3	3.1311953430577604	1.9266587333024152
4	1.4846206593633133	1.2180044341405807
5	0.8271050728055068	0.848211325754849
6	0.5496166711977126	0.6763702953758136
7	0.38953874481212925	0.5592707147459858
8	0.31747348742030673	0.520919755442227
9	0.2392987830474066	0.44172947361695886
>10	2.425535786487965	11.777611038756856
>50	1.1425201892798726	16.95881108091245
>100	0.7658225104815458	26.787729830474756
>500	0.03755346784690777	4.987502891085007
>1k	0.02525917777797964	9.68795558590138
>5k	0.0013411952802467064	1.6610949785990794
>10k+	6.705976401233532E-4	1.731103866908008
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	16921	0.2408802256851691	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	10808	0.15385813363307768	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	10029	0.14276861789472023	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8791	0.12514497157368484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03194463840420303	0.0	0.0	0.0	0.0
2	0.06283584399115515	0.0	0.0	0.0	0.0
3	0.07788285058350924	0.0	0.0	0.0	0.0
4	0.09267361676085638	0.0	0.0	0.0	0.0
5	0.10441796911534278	0.0	0.0	0.0	0.0
6	0.12703830352901418	0.0	0.0	0.0	0.0
7	0.14417794017725857	0.0	0.0	0.0	0.0
8	0.16087627388854653	0.0	0.0	0.0	0.0
9	0.1759232804809006	0.0	0.0	0.0	0.0
10	0.18866412333819194	0.0	0.0	0.0	0.0
11	0.20198862491855468	0.0	0.0	0.0	0.0
12	0.21732034308313877	0.0	0.0	0.0	0.0
13	0.23266629682633433	0.0	0.0	0.0	0.0
14	0.24768483226146545	0.0	0.0	0.0	0.0
15	0.263144670633553	0.0	0.0	0.0	0.0
16	0.27803508586118064	0.0	0.0	0.0	0.0
17	0.29585803028277696	0.0	0.0	0.0	0.0
18	0.3265357021905566	0.0	0.0	0.0	0.0
19	0.34011644418592635	0.0	0.0	0.0	0.0
20	0.3565727730608188	0.0	0.0	0.0	0.0
21	0.3717763710178994	0.0	0.0	0.0	0.0
22	0.3857272380571681	0.0	0.0	0.0	0.0
23	0.40001975898311276	0.0	0.0	0.0	0.0
24	0.41375709234320895	0.0	0.0	0.0	0.0
25	0.4305835462620004	0.0	0.0	0.0	0.0
26	0.45150984682090345	0.0	0.0	0.0	0.0
27	0.46638602646991956	0.0	0.0	0.0	0.0
28	0.4821875187286831	0.0	0.0	0.0	0.0
29	0.4993840976913735	0.0	0.0	0.0	0.0
30	0.5188014269174577	0.0	0.0	0.0	0.0
31	0.5355424673645801	0.0	0.0	0.0	0.0
32	0.5519987962394726	0.0	0.0	0.0	0.0
33	0.5678287596554591	0.0	0.0	0.0	0.0
34	0.5814379728080519	0.0	0.0	0.0	0.0
35	0.5929830270619773	0.0	0.0	0.0	0.0
36	0.6041721918506152	0.0	0.0	0.0	0.0
37	0.6117739908291555	0.0	0.0	0.0	0.0
38	0.6193188474932498	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGTA	35	1.8339051E-8	163.17178	44
CTATCAT	8275	0.0	157.21675	44
TAAGCCA	4260	0.0	153.09804	44
AGCGTTA	110	0.0	103.836586	44
ACCACTC	1040	0.0	101.040985	44
TTAAGCC	3830	0.0	86.54487	43
CCTATCA	8820	0.0	76.14123	43
ACATTGG	1215	0.0	75.20675	44
ACTTCTA	2130	0.0	72.92936	44
GACGGAT	95	0.0	72.6901	43
TCCTATA	65	1.2187229E-10	70.82625	43
ATTAAGC	4795	0.0	70.2302	42
TGCACCG	1435	0.0	70.17845	43
GTAATCA	25	0.0023483522	69.055595	43
GTATAGT	85	3.6241054E-6	67.188385	44
AACGACA	810	0.0	66.78216	43
TCCTCGA	155	9.094947E-12	66.32143	44
TAAGAGT	265	0.0	64.65298	44
CGCTTAT	160	1.0913936E-11	64.248886	44
CCCTATC	11470	0.0	62.9716	42
>>END_MODULE