FastQCFastQC Report
Tue 24 May 2016
ERR840961.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840961.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10437376
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA115210.11038214968973044No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG113110.10837014973878493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA111380.1067126450172917No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC109670.10507430220009321No Hit
AGAACTGCGTCTCGCGACCCAGGCGCGGG105180.10077245468592873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT23750.0238.8253644
CCCATAC7700.0124.9266944
ACGACAC5350.0117.8006744
ACTTCTA15400.099.0797944
AATCGCA9150.097.8792444
TATACCG302.5782356E-885.4214642
TATCCAA4550.076.0176943
CCTATCA28700.075.1580743
ACCACTC6150.072.8126144
CCTATAT2053.6379788E-1272.8126144
AACGACA8900.072.7797743
TGCACCG19750.071.9622543
CTAATCG5800.069.9572342
AAACTAC19500.069.6598543
GTGTTAG22200.069.40272543
TTAGCCC5500.069.3558444
TGTGTTA31550.067.6873742
GTCAAGC4700.067.0461244
CCCTATC34450.066.4526942
CGGATAC1807.0958777E-964.4975944
Produced by FastQC (version 0.11.5)