##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840961.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10437376
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.44362012061269	39.0	37.0	39.0	35.0	39.0
2	37.87664619919796	39.0	38.0	39.0	35.0	39.0
3	37.988524701994066	39.0	38.0	39.0	35.0	40.0
4	38.728003666822005	39.0	38.0	41.0	35.0	41.0
5	39.26486475144711	40.0	39.0	41.0	35.0	41.0
6	39.246997233787496	40.0	39.0	41.0	35.0	41.0
7	39.27490817615462	40.0	39.0	41.0	35.0	41.0
8	39.18441723283706	40.0	38.0	41.0	35.0	41.0
9	39.140441045718774	40.0	38.0	41.0	35.0	41.0
10	39.020929398346865	40.0	38.0	41.0	35.0	41.0
11	38.95540622470629	40.0	38.0	41.0	35.0	41.0
12	38.88976309754482	40.0	38.0	41.0	35.0	41.0
13	38.825372871495674	40.0	38.0	41.0	35.0	41.0
14	38.79398730102279	40.0	38.0	41.0	35.0	41.0
15	38.795002786140884	40.0	38.0	41.0	35.0	41.0
16	38.739735351107406	40.0	38.0	41.0	35.0	41.0
17	38.72741683350298	40.0	38.0	41.0	35.0	41.0
18	38.719534392552305	40.0	38.0	41.0	35.0	41.0
19	38.77785278598759	40.0	38.0	41.0	35.0	41.0
20	38.779204083478454	40.0	38.0	41.0	35.0	41.0
21	38.66854245741458	40.0	38.0	41.0	35.0	41.0
22	38.56581568010964	40.0	37.0	41.0	35.0	41.0
23	38.323798242010255	40.0	37.0	41.0	34.0	41.0
24	38.12670272681563	40.0	36.0	41.0	34.0	41.0
25	38.02134582816353	40.0	36.0	41.0	33.0	41.0
26	37.9352636771236	40.0	35.0	41.0	33.0	41.0
27	37.852297376645886	40.0	35.0	41.0	33.0	41.0
28	37.774221703171825	39.0	35.0	41.0	33.0	41.0
29	37.608400177700986	39.0	35.0	41.0	33.0	41.0
30	37.50572754495687	39.0	35.0	41.0	33.0	41.0
31	37.369496306372916	39.0	35.0	41.0	33.0	41.0
32	37.18327789086836	38.0	35.0	41.0	33.0	41.0
33	37.072558585860754	38.0	35.0	41.0	33.0	41.0
34	36.93567085592972	38.0	35.0	41.0	33.0	41.0
35	36.78500204958169	37.0	35.0	40.0	33.0	41.0
36	36.687673909560125	37.0	35.0	40.0	33.0	41.0
37	36.58310555987456	37.0	35.0	40.0	33.0	41.0
38	36.5175961221046	37.0	35.0	40.0	33.0	41.0
39	36.44163286717542	36.0	35.0	40.0	33.0	41.0
40	36.29309664851171	36.0	35.0	40.0	33.0	41.0
41	36.163811305336424	35.0	35.0	40.0	32.0	41.0
42	36.06471192672343	35.0	35.0	40.0	32.0	41.0
43	35.902177568931236	35.0	35.0	39.0	32.0	41.0
44	35.72374282417679	35.0	35.0	39.0	31.0	41.0
45	35.5290364176454	35.0	34.0	39.0	31.0	41.0
46	35.23708440643242	35.0	34.0	39.0	30.0	41.0
47	35.02987460731654	35.0	34.0	38.0	30.0	41.0
48	35.101905193047884	35.0	34.0	38.0	30.0	41.0
49	34.9699846001197	35.0	33.0	38.0	30.0	40.0
50	35.33283066248997	35.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	14.0
20	33.0
21	107.0
22	241.0
23	471.0
24	1108.0
25	2364.0
26	4696.0
27	9550.0
28	20112.0
29	39584.0
30	73107.0
31	129561.0
32	220079.0
33	381012.0
34	721058.0
35	646444.0
36	1067827.0
37	1595261.0
38	1823354.0
39	1947522.0
40	1753871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.151755766966716	19.401897565058498	37.44634666797479
2	31.319672684015597	18.609351622476762	27.245765602388953	22.825210091118684
3	22.15001165043781	17.586977799784158	31.400018548723356	28.86299200105467
4	23.492264722474307	18.798470036913493	32.708853259669866	25.00041198094234
5	23.659385270780703	18.935314776434232	29.049724758406708	28.355575194378357
6	23.989382005592212	21.361135212528513	28.889425848029237	25.760056933850038
7	33.16288500098109	17.132476591817714	21.654293186333422	28.050345220867772
8	26.824586945991026	18.137901710161636	25.49705021645287	29.540461127394472
9	30.540118512545682	18.31705593436511	24.590960409972777	26.551865143116434
10	26.863035306958377	17.055733165117363	24.74787724424223	31.33335428368203
11	28.624713721149835	17.807473832503497	27.3673383041868	26.20047414215987
12	30.58967119705183	21.758141126658654	20.86376882465478	26.788418851634738
13	27.963321432513304	17.071072269505287	26.946734504917714	28.0188717930637
14	30.951591664418338	19.608386245738394	21.82605091547914	27.61397117436413
15	25.519766653994257	20.945753032179738	21.826347924995705	31.708132388830297
16	29.484977833509113	18.862097140124106	24.860463012925855	26.792462013440925
17	30.006622354124257	19.039842964361924	21.976146111819673	28.977388569694146
18	31.785163244095067	18.096626968310808	18.90098622489024	31.217223562703882
19	31.900680784135783	18.587420823011456	21.944193636408233	27.567704756444535
20	32.89686986460965	18.511942081994555	22.237782753059772	26.353405300336025
21	33.73794332981776	22.231794657967676	18.192321518358636	25.837940493855925
22	33.88188755487969	21.537079817762624	20.13371943293027	24.447313194427412
23	32.723330078364526	21.644539777047413	20.364917389198204	25.267212755389863
24	33.93254204888279	22.031064129528342	20.343226113536584	23.693167708052293
25	31.831891096333337	24.20406592809541	20.228388423798325	23.735654551772928
26	31.739772349170785	24.34395627469372	19.79510609571311	24.12116528042238
27	30.587726677198773	25.25788515705832	19.88328707218205	24.27110109356086
28	31.2867355512571	23.797061352675065	20.391455608400747	24.524747487667085
29	31.192668189006127	24.98153904039486	20.310891480482727	23.514901290116285
30	31.66223958843567	24.94925820615563	19.109203617741954	24.279298587666744
31	28.580385922013406	24.265160704189277	21.44690753367837	25.707545840118947
32	27.944260786416304	23.33536104786766	23.021816300429755	25.69856186528628
33	27.623750545611593	23.273305051121874	22.803446821568652	26.299497581697878
34	28.931884949637325	22.705305685648543	23.69239823487394	24.670411129840193
35	27.610582595544535	23.207166729963845	21.76298315186699	27.41926752262463
36	28.433018122364555	24.04624033208228	22.44509049468027	25.0756510508729
37	26.467111097552127	26.05359470385944	22.505367781110248	24.97392641747819
38	28.446306104488016	24.53696842563222	20.918890577259493	26.097834892620263
39	28.330745306804445	25.549031342200447	20.20286526882632	25.91735808216879
40	27.636258646482055	24.78646544400816	20.836582711862256	26.740693197647524
41	27.351665823125032	24.87946747415457	21.308446868256862	26.460419834463533
42	26.566560378287967	25.070463915818642	21.685419699554345	26.67755600633905
43	26.53495718503262	25.54087945381653	22.238081666413674	25.686081694737183
44	26.634681070704453	26.13197191672489	20.722421524815303	26.510925487755355
45	28.578148460066917	23.96329130686625	21.459709180912604	25.998851052154233
46	27.38314772871332	24.77409837643695	20.914385504874623	26.928368389975116
47	26.50275047673178	24.292683921455186	21.64355102292438	27.561014578888653
48	25.943991905238605	25.036541154514104	20.620932479733067	28.398534460514224
49	27.454153413130676	25.095018912080068	20.114450638767963	27.336377036021293
50	24.05288075698346	28.41082662386414	22.486780619773732	25.04951199937867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159859.0
1	138360.5
2	103698.0
3	85455.5
4	75269.5
5	54413.5
6	31104.0
7	24799.5
8	26127.0
9	25439.0
10	24091.0
11	23433.0
12	23086.0
13	22399.5
14	23032.5
15	24504.0
16	24392.5
17	23576.5
18	23852.0
19	26204.5
20	29005.0
21	32779.5
22	39985.5
23	56364.0
24	77487.5
25	91992.0
26	106764.0
27	120750.0
28	125286.0
29	129487.0
30	134867.5
31	138979.0
32	145552.0
33	150589.5
34	155025.0
35	156927.5
36	157383.0
37	157305.0
38	158139.5
39	171781.5
40	191312.5
41	215670.5
42	245928.5
43	265833.0
44	273101.0
45	272317.0
46	292887.0
47	345876.0
48	406726.0
49	450396.0
50	507439.5
51	578069.0
52	628127.5
53	658635.5
54	664258.0
55	672088.5
56	694844.5
57	718822.0
58	741523.0
59	749888.0
60	747709.5
61	750161.0
62	735096.5
63	714607.5
64	690190.5
65	657419.5
66	631230.5
67	611069.0
68	583174.0
69	546379.5
70	506487.0
71	468459.5
72	418971.5
73	369962.0
74	340882.0
75	313650.0
76	275689.5
77	218891.5
78	173307.0
79	146369.0
80	122345.0
81	93949.5
82	71898.5
83	59372.5
84	47080.5
85	35278.0
86	23697.0
87	15399.0
88	9100.5
89	5200.0
90	3744.0
91	2611.0
92	1914.5
93	1470.0
94	987.5
95	466.5
96	228.0
97	214.0
98	108.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	75117.0
25	65613.0
26	61276.0
27	71510.0
28	75002.0
29	85270.0
30	70804.0
31	79326.0
32	86895.0
33	74564.0
34	68084.0
35	62911.0
36	55214.0
37	72632.0
38	65660.0
39	64437.0
40	76091.0
41	77961.0
42	110581.0
43	166101.0
44	475079.0
45	1021803.0
46	1097806.0
47	1448837.0
48	1557348.0
49	2035390.0
50	1236064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.80288281854476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41128304571505	13.069324696218867
2	12.216283329006568	4.349701211688917
3	4.159843159486944	2.2217160093561326
4	1.9291678056441826	1.3737899352477008
5	1.1101091356661603	0.9881571429030329
6	0.7524155558015135	0.8037099582470744
7	0.536872913258035	0.669051989422848
8	0.4016787027316103	0.5720831100348748
9	0.31922410921854355	0.5114798468344859
>10	2.9474851943968448	12.033377995679619
>50	1.2701823818892122	16.41717210953731
>100	0.8562428615389476	26.270901482655397
>500	0.053659987381345316	6.477512671563818
>1k	0.033392128370434704	10.927151854678213
>5k	0.0013290399351416795	1.6747369107190035
>10k+	8.306499594635498E-4	1.6401330752127568
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	11521	0.11038214968973044	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	11311	0.10837014973878493	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	11138	0.1067126450172917	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	10967	0.10507430220009321	No Hit
AGAACTGCGTCTCGCGACCCAGGCGCGGG	10518	0.10077245468592873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.050089217826396214	0.0	0.0	0.0	0.0
2	0.08501178840352211	0.0	0.0	0.0	0.0
3	0.10747912118908047	0.0	0.0	0.0	0.0
4	0.13140275870103751	0.0	0.0	0.0	0.0
5	0.14698138689264428	0.0	0.0	0.0	0.0
6	0.19240468102327635	0.0	0.0	0.0	0.0
7	0.20952584251060802	0.0	0.0	0.0	0.0
8	0.23015363248387335	0.0	0.0	0.0	0.0
9	0.24890355583625617	0.0	0.0	0.0	0.0
10	0.264271403080621	0.0	0.0	0.0	0.0
11	0.28096142172132155	0.0	0.0	0.0	0.0
12	0.3022311354884599	0.0	0.0	0.0	0.0
13	0.3218529254862525	0.0	0.0	0.0	0.0
14	0.33655010608030217	0.0	0.0	0.0	0.0
15	0.354207800887886	0.0	0.0	0.0	0.0
16	0.3669983720046111	0.0	0.0	0.0	0.0
17	0.380929076426872	0.0	0.0	0.0	0.0
18	0.3956358379730691	0.0	0.0	0.0	0.0
19	0.4119426185278752	0.0	0.0	0.0	0.0
20	0.42615117056240953	0.0	0.0	0.0	0.0
21	0.44346395109268844	0.0	0.0	0.0	0.0
22	0.4620414173064188	0.0	0.0	0.0	0.0
23	0.4823147120502318	0.0	0.0	0.0	0.0
24	0.5048203686443796	0.0	0.0	0.0	0.0
25	0.5296541966103357	0.0	0.0	0.0	0.0
26	0.5613958910745382	0.0	0.0	0.0	0.0
27	0.5861434904711682	0.0	0.0	0.0	0.0
28	0.6106611470162615	0.0	0.0	0.0	0.0
29	0.6364530701969537	0.0	0.0	0.0	0.0
30	0.660568326751858	0.0	0.0	0.0	0.0
31	0.6860440785116872	0.0	0.0	0.0	0.0
32	0.7118551635966741	0.0	0.0	0.0	0.0
33	0.7364303058546516	0.0	0.0	0.0	0.0
34	0.7584185910328419	0.0	0.0	0.0	0.0
35	0.7761912572661941	0.0	0.0	0.0	0.0
36	0.7923255806823477	0.0	0.0	0.0	0.0
37	0.8034394851732849	0.0	0.0	0.0	0.0
38	0.8118228183022246	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	2375	0.0	238.82536	44
CCCATAC	770	0.0	124.92669	44
ACGACAC	535	0.0	117.80067	44
ACTTCTA	1540	0.0	99.07979	44
AATCGCA	915	0.0	97.87924	44
TATACCG	30	2.5782356E-8	85.42146	42
TATCCAA	455	0.0	76.01769	43
CCTATCA	2870	0.0	75.15807	43
ACCACTC	615	0.0	72.81261	44
CCTATAT	205	3.6379788E-12	72.81261	44
AACGACA	890	0.0	72.77977	43
TGCACCG	1975	0.0	71.96225	43
CTAATCG	580	0.0	69.95723	42
AAACTAC	1950	0.0	69.65985	43
GTGTTAG	2220	0.0	69.402725	43
TTAGCCC	550	0.0	69.35584	44
TGTGTTA	3155	0.0	67.68737	42
GTCAAGC	470	0.0	67.04612	44
CCCTATC	3445	0.0	66.45269	42
CGGATAC	180	7.0958777E-9	64.49759	44
>>END_MODULE