FastQCFastQC Report
Tue 24 May 2016
ERR840960.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840960.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6506057
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198510.3051156791279265No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA187550.28826983839828024No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120040.1845049928090086No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79710.12251660260584868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA151.7975353E-5234.8907344
CTATCAT78150.0175.0784944
ACCGTAA450.0117.6091943
CGTGTGC5750.0106.2114544
TAATATC208.888249E-488.2068943
ACTTCTA21950.087.7496144
CCTATCA79800.085.922543
CGCGTAA2350.084.96046444
CGTTCGA5350.082.4363543
GTGTTAG14600.076.5265343
TCTACGA2950.075.6427744
TAAGCCA24750.071.1790144
TACGCTA7450.068.2238342
TCCTATA1900.067.9946844
CCCTATC107200.065.7582142
GGTGCGT6650.065.34553544
TGAACAC12200.065.07065643
GAACACA24200.061.63454444
ACATTGG8450.061.15498444
TCGTGCG15550.060.1281743
Produced by FastQC (version 0.11.5)