##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840960.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6506057
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.43212117569828	39.0	38.0	39.0	35.0	39.0
2	37.83817602581718	39.0	38.0	39.0	35.0	39.0
3	38.06796190073342	39.0	38.0	39.0	35.0	40.0
4	38.72098569071866	39.0	38.0	41.0	35.0	41.0
5	39.37521650978465	41.0	39.0	41.0	35.0	41.0
6	39.36482603825942	41.0	39.0	41.0	35.0	41.0
7	39.355589722008276	41.0	39.0	41.0	35.0	41.0
8	39.29499049885361	40.0	39.0	41.0	35.0	41.0
9	39.193673218663776	40.0	39.0	41.0	35.0	41.0
10	39.12504532315041	40.0	39.0	41.0	35.0	41.0
11	39.00210327084439	40.0	38.0	41.0	35.0	41.0
12	38.97023373757715	40.0	38.0	41.0	35.0	41.0
13	38.8562267437866	40.0	38.0	41.0	35.0	41.0
14	38.83591090579132	40.0	38.0	41.0	35.0	41.0
15	38.8723389604487	40.0	38.0	41.0	35.0	41.0
16	38.815759222521415	40.0	38.0	41.0	35.0	41.0
17	38.82257871395839	40.0	38.0	41.0	35.0	41.0
18	38.85116930269747	40.0	38.0	41.0	35.0	41.0
19	38.93988509476631	40.0	38.0	41.0	35.0	41.0
20	38.878383174325094	40.0	38.0	41.0	35.0	41.0
21	38.85679713534634	40.0	38.0	41.0	35.0	41.0
22	38.80169248440338	40.0	38.0	41.0	35.0	41.0
23	38.722275719379645	40.0	38.0	41.0	35.0	41.0
24	38.533107687190565	40.0	37.0	41.0	35.0	41.0
25	38.488122097536966	40.0	37.0	41.0	35.0	41.0
26	38.41603430192146	40.0	37.0	41.0	34.0	41.0
27	38.36313724916067	40.0	37.0	41.0	34.0	41.0
28	38.2953917377937	40.0	37.0	41.0	34.0	41.0
29	38.1693816669055	40.0	36.0	41.0	34.0	41.0
30	38.07621793111382	40.0	35.0	41.0	34.0	41.0
31	37.968456634064786	40.0	35.0	41.0	33.0	41.0
32	37.85802161760321	40.0	35.0	41.0	33.0	41.0
33	37.73628180734748	39.0	35.0	41.0	33.0	41.0
34	37.674325868646825	39.0	35.0	41.0	33.0	41.0
35	37.507862670066714	39.0	35.0	41.0	33.0	41.0
36	37.45009673783702	39.0	35.0	41.0	33.0	41.0
37	37.38325423777891	39.0	35.0	41.0	33.0	41.0
38	37.30956329354039	39.0	35.0	41.0	33.0	41.0
39	37.22694370899236	38.0	35.0	41.0	33.0	41.0
40	37.15610912157162	38.0	35.0	41.0	33.0	41.0
41	37.05937648819073	38.0	35.0	41.0	33.0	41.0
42	36.97690877703226	37.0	35.0	41.0	33.0	41.0
43	36.87202790047768	37.0	35.0	41.0	33.0	41.0
44	36.72333496614825	37.0	35.0	40.0	33.0	41.0
45	36.55931586319992	37.0	35.0	40.0	32.0	41.0
46	36.296296395012924	37.0	35.0	40.0	31.0	41.0
47	36.13529666624977	36.0	35.0	40.0	31.0	41.0
48	36.149869963034554	36.0	35.0	40.0	31.0	41.0
49	36.237669984667384	36.0	34.0	40.0	31.0	41.0
50	36.35989970285698	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	20.0
21	64.0
22	161.0
23	311.0
24	670.0
25	1374.0
26	2567.0
27	5124.0
28	9864.0
29	19704.0
30	36970.0
31	67369.0
32	114113.0
33	205160.0
34	423226.0
35	325290.0
36	507704.0
37	796183.0
38	985755.0
39	1355499.0
40	1648918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.72843428823325	17.721455560564564	36.55011015120218
2	25.8957307014064	18.38721978611623	31.610989574791613	24.10605993768576
3	24.583876224877834	18.56327111797514	27.414807463260775	29.438045193886254
4	21.72818344505743	19.63355070513523	32.311429180531306	26.326836669276034
5	20.85195380243364	20.505184630260693	30.76815957806702	27.87470198923864
6	23.63635916500578	24.14454715044765	27.495931867796426	24.723161816750146
7	28.272869419988172	20.797404633866563	21.701638949674127	29.228086996471138
8	25.958272422144475	22.222230761273686	24.401061964258844	27.418434852323
9	26.10793296154645	21.413999293273946	26.4264361655608	26.051631579618807
10	28.760999173539364	19.194390703924054	24.32434883370988	27.720261288826702
11	27.399175875649412	20.793731133926432	23.781270283983062	28.02582270644109
12	27.82013745037893	22.210749152674193	23.775306610440087	26.19380678650679
13	28.157730557847866	19.80077948902077	25.763945812340715	26.27754414079065
14	24.77197786616379	23.03024704517652	22.051328477448013	30.146446611211676
15	25.269529609101184	24.050895957413225	23.525677687730067	27.153896745755535
16	26.08955009155315	24.721086827244214	20.997172327263655	28.192190753938984
17	26.195681962208443	26.450167897391612	20.742071580375026	26.61207856002491
18	25.73939945500016	23.743167328537083	20.45195730686036	30.06547590960239
19	28.851514826875942	20.874655724657806	22.64706564974761	27.62676379871864
20	27.759332572708782	21.39048274554004	25.176416376309028	25.673768305442145
21	27.283545164144734	26.798520209706123	19.78949769422555	26.128436931923595
22	30.155084715673407	23.127894514296447	21.18671877605745	25.530301993972692
23	30.587743083099333	23.283195951096033	22.083529240521564	24.04553172528307
24	29.271200667316627	23.12458990137959	22.690486726445833	24.91372270485795
25	28.008540552492466	23.950728151891887	22.232148867981515	25.808582427634136
26	28.97444888535822	25.030658855071746	22.895803336477833	23.0990889230922
27	28.580918051211064	25.336850945169985	20.888543267622374	25.19368773599658
28	27.561589900357504	22.754055210678253	22.133901723941605	27.550453165022642
29	28.34910261535019	25.828610090832072	22.35939947255554	23.462887821262196
30	28.402459801012604	26.14712603430562	20.738193122631994	24.71222104204978
31	28.66744189628597	24.010093546808893	22.195100301088257	25.127364255816882
32	25.894748818970868	24.619835025068404	24.41464709433755	25.070769061623178
33	25.066756732782945	22.38740782966686	24.611730736011992	27.934104701538203
34	28.97530673843062	23.10173305026824	23.62025202467771	24.302708186623427
35	25.318509714291675	23.325853301314385	22.7868575440911	28.568779440302844
36	26.30027804553868	23.9006490786241	25.341153589289828	24.457919286547398
37	24.973010554592385	26.772460466755355	23.051174489191226	25.203354489461038
38	25.930681761213574	23.546017339073206	22.59597351902379	27.92732738068943
39	25.95930094643736	25.20837685702229	24.05250975277435	24.779812443766005
40	25.625114276663048	23.931107162543107	23.172460814406662	27.27131774638718
41	25.033504345094755	24.004816249607373	22.48747808876625	28.474201316531623
42	25.003018938524207	25.220501568997626	22.504477383473226	27.27200210900494
43	25.225252226684663	23.217412064300493	22.83777154459949	28.719564164415353
44	25.826770000524835	24.700652542817654	22.062070292682073	27.41050716397544
45	26.71884467579505	21.88985952013019	26.18641588581842	25.204879918256335
46	25.002511578891255	25.30435210490814	23.398463528799095	26.294672787401513
47	26.106155834661628	23.196015823877236	24.77668598002832	25.92114236143282
48	24.154864629375915	24.25026803170762	21.91550729402921	29.679360044887254
49	24.835583231161078	25.84900853006575	21.6125266649259	27.702881573847275
50	21.64058249494602	26.63415015821547	27.160564589940805	24.564702756897702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101299.0
1	78871.5
2	63029.0
3	62691.5
4	47764.5
5	32434.0
6	19577.5
7	15809.0
8	18509.0
9	19914.0
10	20631.5
11	20377.5
12	19036.0
13	17198.0
14	16349.0
15	16554.5
16	16957.5
17	17870.0
18	18917.0
19	19981.5
20	22024.5
21	23884.0
22	24581.5
23	29680.0
24	37355.0
25	42743.0
26	48140.5
27	55152.0
28	60038.0
29	62350.0
30	68378.5
31	80486.0
32	99117.5
33	115162.5
34	130854.0
35	144301.5
36	146751.0
37	149562.0
38	155934.5
39	163285.0
40	172968.5
41	190392.5
42	214961.0
43	233002.5
44	238359.5
45	240822.5
46	252189.5
47	275234.0
48	309821.0
49	360060.5
50	405920.0
51	427571.0
52	436388.0
53	408699.0
54	387357.5
55	404388.5
56	416753.5
57	423642.0
58	428293.5
59	426116.5
60	417944.0
61	406237.5
62	388783.0
63	368846.0
64	346025.0
65	337139.5
66	336176.5
67	320873.5
68	292005.0
69	262050.5
70	236113.5
71	215440.0
72	200866.5
73	187316.5
74	172702.5
75	156344.5
76	137654.5
77	108818.0
78	83219.0
79	67401.0
80	55358.0
81	44635.5
82	34445.0
83	27194.0
84	21479.0
85	17718.5
86	14280.0
87	11164.5
88	6814.0
89	3139.5
90	1895.0
91	1370.0
92	975.0
93	788.0
94	544.5
95	273.0
96	226.5
97	255.0
98	166.0
99	38.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	29661.0
25	31276.0
26	24476.0
27	27388.0
28	28984.0
29	35630.0
30	31153.0
31	39811.0
32	45945.0
33	37417.0
34	37533.0
35	30589.0
36	27892.0
37	34265.0
38	36772.0
39	34917.0
40	50728.0
41	42070.0
42	74058.0
43	89392.0
44	291337.0
45	547353.0
46	743657.0
47	797394.0
48	1122777.0
49	1114448.0
50	1099134.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.80021813130295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55299006287687	17.34272536368092
2	9.785595856954956	4.266562484527849
3	3.04044337940301	1.9884698666058453
4	1.3465173832905812	1.1741749069330374
5	0.7403881976842585	0.8070312105679542
6	0.49244520053426555	0.6441247673616128
7	0.36249762411481884	0.5531769094447481
8	0.26581924198395396	0.463593396699824
9	0.2031942727625579	0.39867115223297084
>10	2.30903117984449	12.229350529369473
>50	1.1502482490622796	18.21507860785544
>100	0.6989928970081516	25.633575548845368
>500	0.030827847377944282	4.57748286821022
>1k	0.019866834976897424	8.523085821615899
>5k	4.56708850043619E-4	0.6634415523989691
>10k+	6.850632750654284E-4	2.5194550136498703
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19851	0.3051156791279265	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	18755	0.28826983839828024	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12004	0.1845049928090086	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7971	0.12251660260584868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.020427118913959715	0.0	0.0	0.0	0.0
2	0.03682722115714633	0.0	0.0	0.0	0.0
3	0.047832350684907925	0.0	0.0	0.0	0.0
4	0.05949840279604068	0.0	0.0	0.0	0.0
5	0.06955057418033687	0.0	0.0	0.0	0.0
6	0.08678067222589658	0.0	0.0	0.0	0.0
7	0.09930746072467549	0.0	0.0	0.0	0.0
8	0.10986685176597746	0.0	0.0	0.0	0.0
9	0.12127160890228905	0.0	0.0	0.0	0.0
10	0.13209229491841218	0.0	0.0	0.0	0.0
11	0.14260557508180455	0.0	0.0	0.0	0.0
12	0.15468662509412384	0.0	0.0	0.0	0.0
13	0.16555342198815656	0.0	0.0	0.0	0.0
14	0.17534429839763163	0.0	0.0	0.0	0.0
15	0.18842441743132593	0.0	0.0	0.0	0.0
16	0.19922973315481252	0.0	0.0	0.0	0.0
17	0.21349336472152028	0.0	0.0	0.0	0.0
18	0.2407448935661031	0.0	0.0	0.0	0.0
19	0.251473357826407	0.0	0.0	0.0	0.0
20	0.2633699643270878	0.0	0.0	0.0	0.0
21	0.2745441670738513	0.0	0.0	0.0	0.0
22	0.28642540328189564	0.0	0.0	0.0	0.0
23	0.29832200978257645	0.0	0.0	0.0	0.0
24	0.3103569489169861	0.0	0.0	0.0	0.0
25	0.3232218838537689	0.0	0.0	0.0	0.0
26	0.3390532852694036	0.0	0.0	0.0	0.0
27	0.35104211352590364	0.0	0.0	0.0	0.0
28	0.36384556729214024	0.0	0.0	0.0	0.0
29	0.3780784582735749	0.0	0.0	0.0	0.0
30	0.39269560657092306	0.0	0.0	0.0	0.0
31	0.4062675749689866	0.0	0.0	0.0	0.0
32	0.4195475078069559	0.0	0.0	0.0	0.0
33	0.4337803987883906	0.0	0.0	0.0	0.0
34	0.4480747709403714	0.0	0.0	0.0	0.0
35	0.45878786490803874	0.0	0.0	0.0	0.0
36	0.46923966390088495	0.0	0.0	0.0	0.0
37	0.4767864775854254	0.0	0.0	0.0	0.0
38	0.48259644820203695	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTA	15	1.7975353E-5	234.89073	44
CTATCAT	7815	0.0	175.07849	44
ACCGTAA	45	0.0	117.60919	43
CGTGTGC	575	0.0	106.21145	44
TAATATC	20	8.888249E-4	88.20689	43
ACTTCTA	2195	0.0	87.74961	44
CCTATCA	7980	0.0	85.9225	43
CGCGTAA	235	0.0	84.960464	44
CGTTCGA	535	0.0	82.43635	43
GTGTTAG	1460	0.0	76.52653	43
TCTACGA	295	0.0	75.64277	44
TAAGCCA	2475	0.0	71.17901	44
TACGCTA	745	0.0	68.22383	42
TCCTATA	190	0.0	67.99468	44
CCCTATC	10720	0.0	65.75821	42
GGTGCGT	665	0.0	65.345535	44
TGAACAC	1220	0.0	65.070656	43
GAACACA	2420	0.0	61.634544	44
ACATTGG	845	0.0	61.154984	44
TCGTGCG	1555	0.0	60.12817	43
>>END_MODULE