FastQCFastQC Report
Tue 24 May 2016
ERR840959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357388
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT83620.6160360928489128No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC50700.37351147940014207No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG41020.30219804506891174No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG40710.2999142470686348No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC31770.23405245957677537No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA30330.22344384951097254No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29410.2166661264133763No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA29240.2154137210583857No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG27090.19957447686291616No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA25130.1851349798289067No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC24190.17820991492484095No Hit
AGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC23760.17504206608574704No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG23560.17356864802105218No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG23400.17238991356929634No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG22770.1677486466655076No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG22630.1667172540202212No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA18590.13695420911338543No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA18250.1344493984034042No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17610.1297344605963807No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA17240.12700863717669525No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG16540.12185167395026329No Hit
CTCTTTTACCAAGGACCCGCCAACATGGGCCGCGTTCGCACCAAAACCG16280.11993623046616No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG16210.11942053414351682No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC15260.11242179833621632No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC14780.1088855949809487No Hit
AGTCAGCTTTCTGCCGGGCTCCGAACTAGGACTGCTGGGTGCTCCCTCC14610.1076331896259581No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14210.10468635349656841No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCC14060.10358128994804729No Hit
AGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG14020.10328660633510832No Hit
AGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG14010.10321293543187357No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT13780.1015185046574745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGT251.6007107E-10360.3606644
TATGGGT202.2717359E-8360.3606344
TATTCCC600.0360.3606344
ATCTTTA153.2506487E-6360.3606344
AGTAGCG104.6440613E-4360.3606344
TGTAAGT400.0315.3155544
AGTACAA304.7475623E-10300.3005444
CTATAGG256.916889E-8288.288544
ACCGAAA256.916889E-8288.288544
ATTTCGC256.916889E-8288.288544
CTATCAT11100.0253.226444
GGTCATG301.7171806E-7240.2404244
TAAGTCA301.7171806E-7240.2404244
GTCCGTA150.0015641165240.2404244
ATCGTGC600.0210.2103744
AACACCC600.0210.2103744
ATGATAG850.0190.7791744
CCTACTT1500.0180.1803344
GCTTTGA1100.0180.1803344
CCTTGTC305.166576E-5180.1803144
Produced by FastQC (version 0.11.5)