##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840959.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1357388
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.997109153757066	38.0	35.0	39.0	30.0	39.0
2	37.07104158869829	39.0	37.0	39.0	33.0	39.0
3	37.15113217444091	39.0	37.0	39.0	33.0	39.0
4	37.79261493397614	39.0	37.0	40.0	33.0	41.0
5	38.077486319313266	39.0	37.0	40.0	34.0	41.0
6	37.88791045743737	39.0	37.0	40.0	33.0	41.0
7	37.799286570973074	39.0	36.0	40.0	33.0	41.0
8	37.50903278944561	39.0	36.0	40.0	33.0	41.0
9	37.380180906269985	38.0	35.0	40.0	33.0	41.0
10	37.30620353207779	38.0	35.0	40.0	33.0	41.0
11	36.874777882226745	38.0	35.0	40.0	31.0	41.0
12	36.74894429595665	38.0	35.0	40.0	31.0	41.0
13	36.43947051248427	38.0	35.0	40.0	31.0	41.0
14	36.20740348374967	38.0	34.0	40.0	30.0	41.0
15	36.219480354916946	38.0	34.0	40.0	31.0	41.0
16	36.198837031121535	37.0	34.0	40.0	31.0	41.0
17	36.25393107939661	37.0	34.0	40.0	31.0	41.0
18	36.37113853960695	37.0	34.0	40.0	31.0	41.0
19	36.50984685292636	37.0	35.0	40.0	32.0	41.0
20	36.382202435854744	37.0	35.0	40.0	32.0	41.0
21	36.36835304275564	37.0	35.0	40.0	32.0	41.0
22	36.10844209614348	37.0	34.0	40.0	31.0	41.0
23	35.96309014077036	36.0	34.0	40.0	31.0	41.0
24	35.69088131028121	36.0	34.0	39.0	31.0	41.0
25	35.605413609127226	35.0	34.0	39.0	31.0	41.0
26	35.46428178216976	35.0	34.0	39.0	31.0	41.0
27	35.38300340643461	35.0	34.0	39.0	31.0	41.0
28	35.21613449590618	35.0	34.0	39.0	30.0	41.0
29	35.226933078016934	35.0	34.0	39.0	31.0	41.0
30	35.10299867610163	35.0	34.0	38.0	31.0	40.0
31	34.84275571670858	35.0	33.0	38.0	30.0	40.0
32	34.78441192476808	35.0	33.0	38.0	30.0	40.0
33	34.69118996720428	35.0	33.0	37.0	30.0	40.0
34	34.59578048534891	35.0	33.0	37.0	30.0	40.0
35	34.467235865711466	35.0	33.0	37.0	30.0	40.0
36	34.39008302896475	35.0	33.0	37.0	30.0	40.0
37	34.3765879581303	35.0	33.0	36.0	30.0	40.0
38	34.348563710085486	35.0	33.0	36.0	30.0	39.0
39	34.34318479872517	35.0	33.0	36.0	30.0	39.0
40	34.34737380382117	35.0	33.0	36.0	30.0	39.0
41	34.24792489974668	35.0	33.0	36.0	30.0	39.0
42	34.19306468999431	35.0	33.0	35.0	30.0	39.0
43	34.06201425183151	35.0	33.0	35.0	30.0	39.0
44	33.92478912734379	35.0	33.0	35.0	30.0	38.0
45	33.745976707634185	35.0	33.0	35.0	29.0	38.0
46	33.5286513301449	35.0	33.0	35.0	28.0	38.0
47	33.35799175679494	34.0	33.0	35.0	27.0	37.0
48	33.29926137634168	34.0	33.0	35.0	27.0	38.0
49	33.179727178103114	34.0	32.0	35.0	27.0	37.0
50	33.78009293958927	35.0	32.0	36.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	17.0
21	44.0
22	76.0
23	157.0
24	314.0
25	800.0
26	1743.0
27	4023.0
28	8847.0
29	17212.0
30	31131.0
31	54442.0
32	85807.0
33	130859.0
34	179877.0
35	197797.0
36	207388.0
37	176101.0
38	137251.0
39	99465.0
40	24036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.31424765800199	17.865930743457287	43.81982159854073
2	25.593713809168783	18.06498952399756	31.936336552260663	24.404960114572987
3	29.48655800699579	18.0722829434178	27.386053213966825	25.055105835619585
4	26.31944587693423	19.760451691041915	31.335255652768407	22.58484677925545
5	22.929331922781106	18.744456264531586	27.982198162942357	30.34401364974495
6	23.954830895808715	20.362195628663287	30.6723648654622	25.010608610065805
7	34.47009992721315	18.655609155230486	20.200119641546856	26.674171276009513
8	25.863201973201473	18.892019083710775	23.235213513011754	32.009565430075995
9	32.52504074000949	17.62981549858994	25.10313926452864	24.74200449687193
10	35.00436131747149	15.673410992288131	22.53990752828226	26.78232016195811
11	30.54771369718901	19.25610068749687	24.67636372208978	25.519821893224336
12	30.833556801739814	19.648987614447748	21.33413585503924	28.183319728773203
13	31.047055079314095	20.087918855920343	25.360177045914657	23.50484901885091
14	27.020056166696627	22.474782449822747	20.66336228108691	29.84179910239371
15	25.11713673614324	22.69233262707494	22.080053750291	30.110476886490815
16	27.057333643733404	22.26371531205521	20.660636457667223	30.018314586544157
17	26.836615617642117	25.395391737660862	18.38192174971342	29.3860708949836
18	25.857160959136223	21.185615314117996	19.17793585916481	33.77928786758097
19	30.770199824957935	19.736140292974447	21.5552222356467	27.938437646420923
20	28.243361514909516	19.60471140160367	24.27633071752513	27.875596365961684
21	29.862942651622088	22.956000789752082	17.405119243723977	29.775937314901856
22	31.580506089636863	21.142591506628907	21.775203552705637	25.501698851028593
23	32.060177340598266	23.062602586732755	18.72633322233584	26.150886850333137
24	30.530548376735318	22.110774516939887	24.455351012385552	22.903326093939242
25	29.722626262326113	24.129539136752427	20.3735014404298	25.774333160491665
26	30.179926136138093	25.309345179113503	20.98326865938813	23.527460025360273
27	30.54009212977167	22.97424934438006	21.818518973592028	24.667139552256245
28	29.547857392413906	21.57980282845468	20.548146010238337	28.324193768893075
29	28.957066653014472	24.65165893254619	19.622175543266884	26.769098871172453
30	29.522856534472275	23.68327660217934	19.214116645013164	27.57975021833522
31	28.654619178509467	23.718891942998617	21.772111429518297	25.854377448973626
32	27.227506385230843	23.71415272731855	23.284060274373942	25.774280613076666
33	25.748189776633467	22.21848345502489	22.341517246658434	29.69180952168321
34	29.654214077132213	23.490081562783203	22.37961937367838	24.476084986406203
35	26.43023846917181	21.073086911399518	22.51814545271463	29.978529166714036
36	25.575639378703286	24.16723364781342	25.504162910672427	24.752964062810868
37	23.74879832983319	26.763762991447	23.77957758972814	25.707861088991667
38	25.800537297930703	24.045663666142907	22.545873392100987	27.607925643825403
39	26.982565080793712	25.370049149579614	22.80510269021324	24.84228307941343
40	26.915050243109086	23.566299822970187	24.28965836040425	25.228991573516474
41	26.779566055823175	22.479788707719482	22.421534267745972	28.31911096871137
42	26.515595882624698	25.215895740222617	21.49354345858833	26.774964918564354
43	27.504560129632328	23.232434064651137	22.65475912508712	26.60824668062941
44	27.006808900224566	24.630303967796447	21.318395901889243	27.044491230089747
45	27.04567630052247	21.111265987666485	24.738645812805515	27.10441189900553
46	25.066128730595562	24.53999340939251	22.96414798585691	27.429729874155022
47	27.487255134757966	21.43419033128027	24.25537221108654	26.823182322875226
48	23.15326615608229	23.218253857334524	20.75553443649374	32.87294555008945
49	24.365150350180713	25.372017971933236	20.927416613054234	29.335415064831814
50	23.20491680407735	28.6027718346711	24.53836824246058	23.65394311879097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16067.0
1	13065.0
2	11433.0
3	11551.0
4	8631.5
5	5882.5
6	3674.5
7	2950.0
8	3457.0
9	3365.0
10	2969.0
11	3111.0
12	3821.5
13	3880.0
14	3281.5
15	3266.5
16	3707.5
17	3943.0
18	4172.0
19	4206.0
20	4080.0
21	4126.0
22	4086.0
23	4206.5
24	4508.5
25	4703.0
26	5772.0
27	7763.5
28	8919.0
29	9893.5
30	11576.0
31	13163.5
32	15850.0
33	17744.0
34	18723.5
35	20389.0
36	22778.5
37	25325.0
38	25853.0
39	28949.5
40	35011.5
41	41806.0
42	45279.5
43	38263.5
44	33798.5
45	37774.5
46	42060.0
47	45066.0
48	50898.0
49	62155.5
50	70133.0
51	73688.0
52	78970.0
53	82437.5
54	85122.5
55	86849.0
56	89126.0
57	94456.5
58	98843.0
59	97150.0
60	92142.5
61	96106.0
62	100196.5
63	96097.0
64	90015.5
65	87528.5
66	83711.5
67	79094.0
68	84536.5
69	90819.5
70	82532.5
71	65659.0
72	54202.5
73	49089.0
74	46183.0
75	42588.5
76	38197.5
77	31793.5
78	23028.0
79	16049.0
80	12686.0
81	10863.5
82	8514.5
83	5781.5
84	4655.0
85	4394.5
86	3406.0
87	2729.0
88	1640.0
89	859.5
90	516.0
91	276.5
92	237.5
93	224.0
94	244.5
95	206.5
96	148.0
97	148.0
98	74.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11262.0
25	10967.0
26	8552.0
27	9987.0
28	7917.0
29	12531.0
30	13747.0
31	12698.0
32	15600.0
33	12752.0
34	9937.0
35	10057.0
36	9526.0
37	10287.0
38	10510.0
39	9901.0
40	13864.0
41	11411.0
42	16660.0
43	27295.0
44	71672.0
45	142007.0
46	144415.0
47	181417.0
48	190042.0
49	235612.0
50	146762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.266651277159134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.43792241521753	6.063904073236158
2	11.818662288158482	2.190388439737526
3	5.181951876289011	1.440580229177722
4	2.835143747250856	1.050891537055675
5	1.773556528704635	0.8217464935917363
6	1.2393939068360595	0.6891018677811371
7	0.8758927149013165	0.5681614641636266
8	0.7274249867433645	0.5392634945954292
9	0.5622978172237798	0.468955600750848
>10	4.717649285472921	9.655240889609138
>50	1.87589260681824	12.814672215338163
>100	2.814636483614613	50.49447657946947
>500	0.10448211373009755	6.415613842250408
>1k	0.03349808226461143	5.794266390239589
>5k	0.0015951467745053062	0.9927368830033694
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	8362	0.6160360928489128	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	5070	0.37351147940014207	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	4102	0.30219804506891174	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG	4071	0.2999142470686348	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	3177	0.23405245957677537	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	3033	0.22344384951097254	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2941	0.2166661264133763	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	2924	0.2154137210583857	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	2709	0.19957447686291616	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	2513	0.1851349798289067	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	2419	0.17820991492484095	No Hit
AGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	2376	0.17504206608574704	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	2356	0.17356864802105218	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	2340	0.17238991356929634	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	2277	0.1677486466655076	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	2263	0.1667172540202212	No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA	1859	0.13695420911338543	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1825	0.1344493984034042	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1761	0.1297344605963807	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1724	0.12700863717669525	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG	1654	0.12185167395026329	No Hit
CTCTTTTACCAAGGACCCGCCAACATGGGCCGCGTTCGCACCAAAACCG	1628	0.11993623046616	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1621	0.11942053414351682	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	1526	0.11242179833621632	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	1478	0.1088855949809487	No Hit
AGTCAGCTTTCTGCCGGGCTCCGAACTAGGACTGCTGGGTGCTCCCTCC	1461	0.1076331896259581	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1421	0.10468635349656841	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCC	1406	0.10358128994804729	No Hit
AGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	1402	0.10328660633510832	No Hit
AGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	1401	0.10321293543187357	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	1378	0.1015185046574745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.013408104388723047	0.0	0.0	0.0	0.0
2	0.027773930519497742	0.0	0.0	0.0	0.0
3	0.03668810981090152	0.0	0.0	0.0	0.0
4	0.04825444161875602	0.0	0.0	0.0	0.0
5	0.05738963361986403	0.0	0.0	0.0	0.0
6	0.07138710523446502	0.0	0.0	0.0	0.0
7	0.08192204439703313	0.0	0.0	0.0	0.0
8	0.09194128723695803	0.0	0.0	0.0	0.0
9	0.10026609930248388	0.0	0.0	0.0	0.0
10	0.1066017969806717	0.0	0.0	0.0	0.0
11	0.11367420369120694	0.0	0.0	0.0	0.0
12	0.12391445924083608	0.0	0.0	0.0	0.0
13	0.13349167666135253	0.0	0.0	0.0	0.0
14	0.14321623588833848	0.0	0.0	0.0	0.0
15	0.1505833262118127	0.0	0.0	0.0	0.0
16	0.16185497440672822	0.0	0.0	0.0	0.0
17	0.17283193898870478	0.0	0.0	0.0	0.0
18	0.19419650092677995	0.0	0.0	0.0	0.0
19	0.20207928757289736	0.0	0.0	0.0	0.0
20	0.21121447957400538	0.0	0.0	0.0	0.0
21	0.22278081138185987	0.0	0.0	0.0	0.0
22	0.23397878867354066	0.0	0.0	0.0	0.0
23	0.2461344877072731	0.0	0.0	0.0	0.0
24	0.25585904693425904	0.0	0.0	0.0	0.0
25	0.26676234061300086	0.0	0.0	0.0	0.0
26	0.2827489266149399	0.0	0.0	0.0	0.0
27	0.29239981493869105	0.0	0.0	0.0	0.0
28	0.30426083035948454	0.0	0.0	0.0	0.0
29	0.31707956752232963	0.0	0.0	0.0	0.0
30	0.330119317394879	0.0	0.0	0.0	0.0
31	0.34411678900947995	0.0	0.0	0.0	0.0
32	0.35605147533350817	0.0	0.0	0.0	0.0
33	0.36879654159311853	0.0	0.0	0.0	0.0
34	0.37896312623951295	0.0	0.0	0.0	0.0
35	0.38883502727296837	0.0	0.0	0.0	0.0
36	0.3967178139190858	0.0	0.0	0.0	0.0
37	0.40320085340374306	0.0	0.0	0.0	0.0
38	0.4072527530816539	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGGT	25	1.6007107E-10	360.36066	44
TATGGGT	20	2.2717359E-8	360.36063	44
TATTCCC	60	0.0	360.36063	44
ATCTTTA	15	3.2506487E-6	360.36063	44
AGTAGCG	10	4.6440613E-4	360.36063	44
TGTAAGT	40	0.0	315.31555	44
AGTACAA	30	4.7475623E-10	300.30054	44
CTATAGG	25	6.916889E-8	288.2885	44
ACCGAAA	25	6.916889E-8	288.2885	44
ATTTCGC	25	6.916889E-8	288.2885	44
CTATCAT	1110	0.0	253.2264	44
GGTCATG	30	1.7171806E-7	240.24042	44
TAAGTCA	30	1.7171806E-7	240.24042	44
GTCCGTA	15	0.0015641165	240.24042	44
ATCGTGC	60	0.0	210.21037	44
AACACCC	60	0.0	210.21037	44
ATGATAG	85	0.0	190.77917	44
CCTACTT	150	0.0	180.18033	44
GCTTTGA	110	0.0	180.18033	44
CCTTGTC	30	5.166576E-5	180.18031	44
>>END_MODULE