FastQCFastQC Report
Tue 24 May 2016
ERR840958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17556353
Sequences flagged as poor quality0
Sequence length24-50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA1340470.7635241783985547No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC240680.13708997534966402No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT191290.10895770892736094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA431500.0264.5815444
CTATCAT72450.0215.4732744
AATCGCA7100.0122.1520944
TTAAGCC418300.0117.18255643
ACCACTC8100.095.99522444
ATTAGTC5650.095.276444
CGACGGG101600.094.6342944
TAAGATC7100.084.2428244
CATCGAT1600.084.111244
ACTTCTA30600.084.0501244
ATTAAGC592100.080.1746642
ACATTGG14800.078.8068944
GATTAAG626350.061.63038341
ACCTTAG45200.059.9723842
TCGCAAA23200.059.15900443
CTAATTC5600.055.34229343
AACCTTA60700.055.1371241
TATCATA15400.054.37491244
AGATTAA759250.052.93203740
ATCATAC2054.486901E-851.05937244
Produced by FastQC (version 0.11.5)