FastQCFastQC Report
Tue 24 May 2016
ERR840957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438936
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC105640.30718803723012No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79000.22972221640646992No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA63800.18552249881940228No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT50450.1467023521228659No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA45720.13294809790004813No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44680.12992390669672246No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36010.10471262041515167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG202.7903297E-9548.655744
CTATCAT22250.0453.7197644
ATGCGGT254.668733E-6329.1934244
AATCGCA950.0288.7661444
GTTAACA551.4479156E-9249.3889344
GGTCAAT1100.0224.4500344
TCCCGTA2900.0208.1107844
AATACCT1400.0195.9484644
TATAATT1051.8189894E-12182.8852244
CCCATAC2150.0165.8726544
GCACGAA902.7504939E-8152.4043644
AACGACA3150.0146.5927143
GTATAGG1150.0143.1275644
ACCTGTT1351.2732926E-11142.2440844
CGCATTG601.5332643E-4137.1639344
ACAAGTG1401.8189894E-11137.1639344
TCGCAAA3650.0128.6200643
CACGAAC651.3442332E-9126.61285444
TACTATA2400.0125.73360444
ATCCCTA2650.0124.2239344
Produced by FastQC (version 0.11.5)