##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840957.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3438936
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.076336983299484	39.0	37.0	39.0	33.0	39.0
2	37.623913908255346	39.0	37.0	39.0	35.0	39.0
3	38.07405546366667	39.0	38.0	39.0	35.0	41.0
4	38.74678999551024	40.0	38.0	41.0	35.0	41.0
5	39.1097851195835	40.0	38.0	41.0	35.0	41.0
6	39.115536607834514	40.0	38.0	41.0	35.0	41.0
7	39.069184189528386	40.0	38.0	41.0	35.0	41.0
8	38.971471408598475	40.0	38.0	41.0	35.0	41.0
9	38.83306551793927	40.0	38.0	41.0	35.0	41.0
10	38.7566232695229	40.0	38.0	41.0	35.0	41.0
11	38.62940426922746	40.0	38.0	41.0	35.0	41.0
12	38.554404908960215	40.0	38.0	41.0	35.0	41.0
13	38.47945032998579	40.0	37.0	41.0	35.0	41.0
14	38.43769962569818	40.0	37.0	41.0	34.0	41.0
15	38.43700551565949	40.0	37.0	41.0	34.0	41.0
16	38.388566986998306	40.0	37.0	41.0	34.0	41.0
17	38.37090512879565	40.0	37.0	41.0	34.0	41.0
18	38.430663146973366	40.0	37.0	41.0	35.0	41.0
19	38.49385682083063	40.0	37.0	41.0	35.0	41.0
20	38.397682597175404	40.0	37.0	41.0	35.0	41.0
21	38.327942712513405	40.0	36.0	41.0	34.0	41.0
22	38.166572451479176	40.0	36.0	41.0	34.0	41.0
23	38.05759077807787	40.0	35.0	41.0	34.0	41.0
24	37.796632155992434	39.0	35.0	41.0	33.0	41.0
25	37.7209118673673	39.0	35.0	41.0	33.0	41.0
26	37.62539523641295	39.0	35.0	41.0	33.0	41.0
27	37.522567896424654	39.0	35.0	41.0	33.0	41.0
28	37.37234358864794	39.0	35.0	41.0	33.0	41.0
29	37.21830718485724	39.0	35.0	41.0	33.0	41.0
30	37.09806376491489	38.0	35.0	41.0	33.0	41.0
31	36.91139826288291	38.0	35.0	41.0	33.0	41.0
32	36.78044762162936	37.0	35.0	40.0	33.0	41.0
33	36.65751291654373	37.0	35.0	40.0	32.0	41.0
34	36.58067396873834	37.0	35.0	40.0	32.0	41.0
35	36.41852224763288	37.0	35.0	40.0	32.0	41.0
36	36.321376736666274	36.0	35.0	40.0	32.0	41.0
37	36.26418482156673	36.0	35.0	40.0	32.0	41.0
38	36.20228355976964	36.0	35.0	40.0	32.0	41.0
39	36.080708914897286	35.0	35.0	40.0	32.0	41.0
40	35.972216855165925	35.0	35.0	39.0	32.0	41.0
41	35.882320836662394	35.0	35.0	39.0	32.0	41.0
42	35.79720043256134	35.0	35.0	39.0	32.0	41.0
43	35.68346391791046	35.0	35.0	39.0	32.0	41.0
44	35.44793184946024	35.0	34.0	39.0	31.0	41.0
45	35.29038251122078	35.0	34.0	39.0	31.0	41.0
46	35.01968825576027	35.0	34.0	38.0	30.0	41.0
47	34.976501599986406	35.0	34.0	38.0	30.0	40.0
48	35.00678663168826	35.0	34.0	38.0	30.0	40.0
49	35.08084675340567	35.0	34.0	38.0	30.0	40.0
50	36.05078348068737	37.0	34.0	39.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	36.0
22	115.0
23	199.0
24	497.0
25	1094.0
26	2164.0
27	4435.0
28	8982.0
29	16729.0
30	30433.0
31	52866.0
32	87772.0
33	154070.0
34	312527.0
35	234116.0
36	358404.0
37	520430.0
38	552093.0
39	572652.0
40	529313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.795205261162174	17.108111346067503	42.09668339277032
2	28.736417310470447	17.56790472401929	27.64346297808392	26.052214987426343
3	24.94445956540046	17.39901527681818	27.188409438268113	30.468115719513243
4	21.65815240527884	18.177511881581978	32.82963102541018	27.334704687728994
5	24.33607371582373	20.171413483705425	29.87996868798954	25.6125441124813
6	24.505835525871955	21.292138033391726	26.710761700712084	27.491264740024242
7	30.565558649535784	19.273839350310677	21.63299927652041	28.527602723633123
8	29.96141829914834	19.264243358992434	22.4725903593437	28.301747982515522
9	29.064774686123844	18.624975864627896	25.54883254588047	26.761416903367785
10	32.12874563527789	16.130337988261488	23.32704068932949	28.413875687131135
11	31.27292860349829	17.324195623297438	22.25563371926666	29.14724205393761
12	29.53762442802076	18.492900129574963	22.437783081743888	29.53169236066039
13	29.540386910369953	17.53289389508848	26.261204046833093	26.66551514770848
14	26.885874002889267	20.764562062219245	21.196759695440683	31.152804239450806
15	26.83042080457444	22.09453738016642	22.635693132992298	28.43934868226684
16	28.36769861375728	20.8780273898671	20.817747117131578	29.936526879244045
17	29.13188846782842	22.345487092519313	18.54713201990383	29.975492419748434
18	26.71262274145259	19.650351155124724	19.401088010942917	34.23593809247977
19	30.661634877764516	17.506664852151946	22.227543635589615	29.604156634493922
20	30.66032633349385	17.84665373243352	23.37804483712404	28.11497509694859
21	31.509193541258114	22.531591166570124	18.43119499752249	27.528020294649274
22	32.120138321853034	19.977836167931013	21.71421625758665	26.1878092526293
23	32.98630739275171	20.875381222564187	20.12288103064436	26.015430354039736
24	32.06326026422126	21.022025417163913	22.654914194390358	24.25980012422447
25	31.248555866822116	21.649099182627936	21.221284782770198	25.881060167779758
26	30.9934122855848	22.952632763871556	21.383796232887782	24.670158717655863
27	30.073714642428644	21.82496498738956	21.969767404108246	26.131552966073556
28	30.924996142387446	20.83051430877518	20.407126494083016	27.83736305475436
29	32.06049872278021	22.358341663676097	20.41332222422091	25.167837389322784
30	31.958562408469977	22.865880008642836	20.01917628982306	25.156381293064122
31	29.9177385740043	22.533494742855588	21.59332961547355	25.95543706766656
32	28.95946855592072	20.392095348036783	23.017364527143304	27.63107156889919
33	27.72556532665249	19.226936435498505	23.999127462204132	29.048370775644873
34	30.04407519555579	20.6856878320978	24.132842837718748	25.137394134627662
35	27.73926670085253	20.18986875874141	21.965006905793004	30.10585763461306
36	27.631073134467464	20.33987554905061	25.31267288994636	26.71637842653556
37	27.360715943575865	22.77889179058524	24.35089192952983	25.509500336309067
38	28.582105698558657	21.07671259028286	23.02236787680168	27.318813834356803
39	28.499938178633638	22.378629923206972	21.509607296586882	27.611824601572504
40	28.492391915460637	21.421693967690356	22.159326415260487	27.926587701588517
41	28.55284547553687	20.638236591124702	22.338443413496663	28.470474519841765
42	26.321534634125204	22.552184113022737	22.00783836861081	29.11844288424125
43	28.596140000526404	20.212673450601248	22.028263839816255	29.162922709056094
44	27.529112535221806	22.61317518349155	21.537554495803445	28.320157785483204
45	28.699878674199287	19.580942172997194	24.832302436192304	26.886876716611212
46	28.22229596100784	21.853912646870793	22.479169148944802	27.444622243176564
47	27.441324818614127	21.623688760539746	23.51853826189921	27.41644815894692
48	25.69874842870416	21.910285839208612	20.93628736951413	31.4546783625731
49	25.746597157622446	23.02296359159773	21.5582655673028	29.67217368347702
50	22.959284924527463	29.442592675093454	27.36451595919177	20.23360644118731
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35190.0
1	27593.5
2	24721.5
3	25538.0
4	18207.5
5	10953.0
6	5336.0
7	4714.0
8	6540.5
9	7291.5
10	7527.0
11	7913.5
12	7758.0
13	6983.5
14	7167.5
15	7915.5
16	7787.0
17	6924.0
18	6322.5
19	6031.5
20	5058.0
21	4226.0
22	4620.0
23	6687.0
24	8898.0
25	10723.0
26	13138.5
27	14739.5
28	14806.0
29	16242.0
30	19696.0
31	24005.5
32	30721.5
33	36894.0
34	40967.5
35	42213.0
36	44622.0
37	50649.5
38	55373.5
39	62577.5
40	71486.0
41	81588.5
42	97844.5
43	106058.5
44	105180.5
45	106307.5
46	111910.0
47	124543.5
48	139208.5
49	158341.0
50	190391.5
51	212345.5
52	214806.5
53	217951.0
54	220267.0
55	222010.0
56	230015.5
57	240356.0
58	245176.5
59	249522.0
60	257890.0
61	257436.5
62	245235.0
63	231516.0
64	218055.5
65	209721.0
66	206423.5
67	203927.5
68	202046.0
69	199747.0
70	187341.5
71	170609.0
72	154201.5
73	138314.0
74	123732.0
75	108284.0
76	91342.5
77	72745.0
78	61040.0
79	50174.5
80	37700.0
81	29288.5
82	25940.0
83	23463.5
84	20029.0
85	15370.0
86	11206.5
87	8477.5
88	5368.5
89	2573.5
90	1337.0
91	1076.5
92	983.5
93	887.5
94	783.5
95	523.5
96	314.5
97	293.0
98	146.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	19930.0
25	16612.0
26	14329.0
27	18105.0
28	17757.0
29	19963.0
30	23885.0
31	25225.0
32	32841.0
33	23077.0
34	21208.0
35	19367.0
36	19907.0
37	23830.0
38	21002.0
39	21709.0
40	27504.0
41	26643.0
42	44539.0
43	73984.0
44	240539.0
45	345381.0
46	400020.0
47	477819.0
48	581189.0
49	635660.0
50	246911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.68160344140275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72873783367916	9.096354085590612
2	11.002275768355114	2.7905299649446857
3	4.148089287433857	1.5781327014830124
4	2.0431717579988016	1.0364277599045804
5	1.2660953501594967	0.8028059574863347
6	0.906116329109893	0.6894604774530747
7	0.6635296559753667	0.5890233978082996
8	0.5072943150164497	0.5146644264893326
9	0.4399979119765508	0.5021891131258666
>10	3.68885487123489	10.288304319314042
>50	1.6136857167754042	15.092913188439686
>100	1.8828953265235007	41.09864581547368
>500	0.07270624825857805	6.199692038291885
>1k	0.03497760051358619	8.231202229161273
>5k	0.0011790202420309953	0.9631494428142314
>10k+	3.9300674734366514E-4	0.5265050822193812
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	10564	0.30718803723012	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7900	0.22972221640646992	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	6380	0.18552249881940228	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	5045	0.1467023521228659	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4572	0.13294809790004813	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4468	0.12992390669672246	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3601	0.10471262041515167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.026985090737367604	0.0	0.0	0.0	0.0
2	0.04498484414946949	0.0	0.0	0.0	0.0
3	0.05481346556027795	0.0	0.0	0.0	0.0
4	0.06621234009589012	0.0	0.0	0.0	0.0
5	0.07807647481662933	0.0	0.0	0.0	0.0
6	0.09395347863408915	0.0	0.0	0.0	0.0
7	0.10657366115565978	0.0	0.0	0.0	0.0
8	0.11747819674457448	0.0	0.0	0.0	0.0
9	0.12835365357191875	0.0	0.0	0.0	0.0
10	0.13815319622115677	0.0	0.0	0.0	0.0
11	0.1474293211621269	0.0	0.0	0.0	0.0
12	0.15629834344111085	0.0	0.0	0.0	0.0
13	0.1658361772362149	0.0	0.0	0.0	0.0
14	0.17511230217718504	0.0	0.0	0.0	0.0
15	0.18610407405081106	0.0	0.0	0.0	5.8157523140878455E-5
16	0.1950894113760768	0.0	0.0	0.0	5.8157523140878455E-5
17	0.2085819567447606	0.0	0.0	0.0	5.8157523140878455E-5
18	0.2305945792535831	0.0	0.0	0.0	5.8157523140878455E-5
19	0.24042320066439155	0.0	0.0	0.0	5.8157523140878455E-5
20	0.25156036634586976	0.0	0.0	0.0	5.8157523140878455E-5
21	0.265081990476124	0.0	0.0	0.0	5.8157523140878455E-5
22	0.27496876941007337	0.0	0.0	0.0	5.8157523140878455E-5
23	0.28767618821635527	0.0	0.0	0.0	5.8157523140878455E-5
24	0.2999765043606511	0.0	0.0	0.0	5.8157523140878455E-5
25	0.3137307585834688	0.0	0.0	0.0	5.8157523140878455E-5
26	0.32600199596619417	0.0	0.0	0.0	5.8157523140878455E-5
27	0.33792428821007425	0.0	0.0	0.0	5.8157523140878455E-5
28	0.3494976353151091	0.0	0.0	0.0	5.8157523140878455E-5
29	0.3632809682994973	0.0	0.0	0.0	5.8157523140878455E-5
30	0.3784310030776961	0.0	0.0	0.0	5.8157523140878455E-5
31	0.3904405316062875	0.0	0.0	0.0	5.8157523140878455E-5
32	0.4044274159216688	0.0	0.0	0.0	5.8157523140878455E-5
33	0.41608799931141494	0.0	0.0	0.0	5.8157523140878455E-5
34	0.4264691171920617	0.0	0.0	0.0	5.8157523140878455E-5
35	0.4350473518553413	0.0	0.0	0.0	5.8157523140878455E-5
36	0.44356742899548	0.0	0.0	0.0	5.8157523140878455E-5
37	0.45153500966578036	0.0	0.0	0.0	5.8157523140878455E-5
38	0.4561585327554802	0.0	0.0	0.0	5.8157523140878455E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATG	20	2.7903297E-9	548.6557	44
CTATCAT	2225	0.0	453.71976	44
ATGCGGT	25	4.668733E-6	329.19342	44
AATCGCA	95	0.0	288.76614	44
GTTAACA	55	1.4479156E-9	249.38893	44
GGTCAAT	110	0.0	224.45003	44
TCCCGTA	290	0.0	208.11078	44
AATACCT	140	0.0	195.94846	44
TATAATT	105	1.8189894E-12	182.88522	44
CCCATAC	215	0.0	165.87265	44
GCACGAA	90	2.7504939E-8	152.40436	44
AACGACA	315	0.0	146.59271	43
GTATAGG	115	0.0	143.12756	44
ACCTGTT	135	1.2732926E-11	142.24408	44
CGCATTG	60	1.5332643E-4	137.16393	44
ACAAGTG	140	1.8189894E-11	137.16393	44
TCGCAAA	365	0.0	128.62006	43
CACGAAC	65	1.3442332E-9	126.612854	44
TACTATA	240	0.0	125.733604	44
ATCCCTA	265	0.0	124.22393	44
>>END_MODULE