FastQCFastQC Report
Tue 24 May 2016
ERR840956.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840956.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7190994
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA166570.231636961454842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA119760.16654164917951536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA117830.16385773649651217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA109840.15274661611454549No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA96340.13397313361685464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA96190.1337645393668803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA93030.12937015383408745No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC83870.11663199830232093No Hit
AAAAAAAAAAAAAAAAAAAAAAAA72600.10095961698758198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT67100.0178.1329244
TAAGCCA42450.0146.6682744
ACCACTC6000.0146.1257844
ACATTGG12950.0117.4091244
GTTCGAA1200.0110.9816144
ACGAACA1700.0110.981644
TTAAGCC31200.0105.06892443
AATCGCA8600.0103.238744
ACTTCTA26450.0101.1212344
GTGTTAG24200.092.4687843
CGGTCTA1850.089.9850944
TAGATTA351.0300628E-683.0331943
CCTATCA75400.083.0221743
GGATATC553.9147766E-580.713944
AAATTCC1400.079.27257544
TATTCGT150.001414814478.46409642
AGGTACT3100.075.1810844
GATTCAA450.001785984673.9877444
TGCACCG11950.072.9580343
TAACGAA600.071.9254242
Produced by FastQC (version 0.11.5)