##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840956.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7190994
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.53672844115848	39.0	38.0	39.0	35.0	39.0
2	37.911863784061005	39.0	38.0	39.0	35.0	39.0
3	38.04793885240343	39.0	38.0	39.0	35.0	39.0
4	38.56309002621891	39.0	38.0	41.0	35.0	41.0
5	39.32154706289562	41.0	39.0	41.0	35.0	41.0
6	39.290891078479554	40.0	39.0	41.0	35.0	41.0
7	39.28030074840836	40.0	39.0	41.0	35.0	41.0
8	39.21841208600647	40.0	39.0	41.0	35.0	41.0
9	39.106373333088584	40.0	38.0	41.0	35.0	41.0
10	39.011030463938646	40.0	38.0	41.0	35.0	41.0
11	38.81508036302075	40.0	38.0	41.0	35.0	41.0
12	38.828325958831286	40.0	38.0	41.0	35.0	41.0
13	38.67127924178493	40.0	38.0	41.0	35.0	41.0
14	38.62779832662911	40.0	38.0	41.0	35.0	41.0
15	38.592175991246826	40.0	38.0	41.0	35.0	41.0
16	38.5184299972994	40.0	38.0	41.0	35.0	41.0
17	38.49795758416708	40.0	37.0	41.0	35.0	41.0
18	38.516050493158524	40.0	37.0	41.0	35.0	41.0
19	38.57807710589106	40.0	37.0	41.0	35.0	41.0
20	38.53896304182704	40.0	37.0	41.0	35.0	41.0
21	38.47414974897768	40.0	37.0	41.0	35.0	41.0
22	38.37288433281964	40.0	37.0	41.0	34.0	41.0
23	38.20673400645307	40.0	36.0	41.0	34.0	41.0
24	37.994206503301214	40.0	35.0	41.0	33.0	41.0
25	37.90396394095407	40.0	35.0	41.0	33.0	41.0
26	37.81886601269833	39.0	35.0	41.0	33.0	41.0
27	37.720745455160014	39.0	35.0	41.0	33.0	41.0
28	37.56568379990001	39.0	35.0	41.0	33.0	41.0
29	37.41297139231268	39.0	35.0	41.0	33.0	41.0
30	37.31270357265053	39.0	35.0	41.0	33.0	41.0
31	37.134718523369045	38.0	35.0	41.0	33.0	41.0
32	37.024329172231546	38.0	35.0	41.0	33.0	41.0
33	36.87720351113847	37.0	35.0	40.0	33.0	41.0
34	36.741562402295415	37.0	35.0	40.0	33.0	41.0
35	36.560931317055875	37.0	35.0	40.0	32.0	41.0
36	36.48063249521662	37.0	35.0	40.0	33.0	41.0
37	36.37452635147205	36.0	35.0	40.0	33.0	41.0
38	36.297521594361235	36.0	35.0	40.0	33.0	41.0
39	36.19505020772815	35.0	35.0	40.0	33.0	41.0
40	36.10624728388257	35.0	35.0	40.0	32.0	41.0
41	35.97878679242563	35.0	35.0	39.0	32.0	41.0
42	35.88122196047834	35.0	35.0	39.0	32.0	41.0
43	35.751851863689026	35.0	35.0	39.0	32.0	41.0
44	35.585891744081245	35.0	35.0	39.0	31.0	41.0
45	35.38914104453635	35.0	34.0	39.0	31.0	41.0
46	35.1336935674729	35.0	34.0	38.0	31.0	41.0
47	34.94399289756169	35.0	34.0	38.0	30.0	40.0
48	34.92449848057858	35.0	34.0	38.0	30.0	40.0
49	34.98630045772713	35.0	34.0	38.0	30.0	40.0
50	35.09260107814604	35.0	34.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	16.0
21	49.0
22	132.0
23	322.0
24	823.0
25	1799.0
26	3833.0
27	7809.0
28	15550.0
29	29949.0
30	55413.0
31	96454.0
32	158069.0
33	265672.0
34	500705.0
35	515293.0
36	811115.0
37	1179936.0
38	1260701.0
39	1200643.0
40	1086709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.82922249691767	19.144641199811876	41.02613630327045
2	26.220950817091488	20.61553660036429	30.81763105350943	22.345881529034788
3	25.105402674512035	17.335447644651072	30.04243919547145	27.516710485365444
4	21.521822991369483	20.097318952011364	32.911249821651914	25.46960823496724
5	23.00567904798697	18.65342955368896	30.180528588954463	28.16036280936961
6	23.60170235158032	22.00338367686025	28.64930773130947	25.74560624024996
7	29.823262263881738	18.487903063192654	22.19060396935389	29.498230703571714
8	25.61684796288246	20.052332125433566	23.416262063353134	30.914557848330844
9	27.795461934747824	20.83526978328726	24.882262452172814	26.4870058297921
10	30.219883370782952	16.90354629693753	23.48949255137746	29.387077780902054
11	27.886297777469988	20.573289311602817	24.37365404560204	27.166758865325157
12	30.813389637093287	19.343195113220787	23.06181871379673	26.781596535889197
13	29.812512706866396	19.885081255804135	23.34312891931213	26.959277118017344
14	26.133772326885545	20.262275284891075	21.32481267541038	32.279139712813
15	26.486296609342187	22.16977235692312	23.20964250561188	28.134288528122813
16	28.184518023516635	22.123144588912186	20.84930122316887	28.843036164402307
17	27.809771500296065	23.618334266444947	18.833070921766865	29.738823311492123
18	26.213872518875693	21.42182290793178	20.40774891482318	31.956555658369346
19	29.755343976090092	21.650539549886982	20.955447883839145	27.63866859018378
20	28.130895394989903	20.83695244357039	22.28570904105886	28.746443120380853
21	27.67795384059561	25.931380279277107	19.399153997347238	26.991511882780046
22	31.237280965607816	22.305525494806417	21.48683478250712	24.970358757078646
23	31.610052796595294	23.32406340486447	20.43646538990298	24.62941840863725
24	30.49679919076556	23.249609163906964	22.16541969024032	24.088171955087155
25	30.551445525723008	25.197245218530306	19.136210119023506	25.115099136723178
26	30.273935678530954	26.369925359548517	20.79747726846148	22.558661693459047
27	29.707970792818696	25.95298697027047	19.689269652884235	24.649772584026604
28	28.672840190512837	23.768987090530793	20.802362599517497	26.755810119438877
29	29.610532964679376	25.963805801815866	20.532080867225897	23.89358036627886
30	29.76361273488174	26.118842333667736	19.1828254747032	24.934719456747327
31	27.431792367205567	26.181487339827708	21.217206996698977	25.169513296267752
32	28.05678049223156	24.367857543768466	23.728032532196757	23.847329431803217
33	26.2313059913442	23.334365519760375	23.409661116298473	27.02466737259695
34	28.08929082167713	24.453426801464882	23.102067157744536	24.35521521911345
35	26.17982749501067	23.475503290332735	21.18342813407887	29.16124108057772
36	26.62779811564003	23.89964010526019	25.25331682716409	24.21924495193569
37	24.19207678608554	26.495860548118422	23.26765813535358	26.044404530442456
38	26.562065387638366	24.600841267617884	21.586490746194283	27.250602598549467
39	27.484608653138398	26.02236311915849	21.47464975079573	25.018378476907383
40	26.610681931084667	25.325883559316896	21.805080140526766	26.258354369071675
41	26.508972829871286	25.233980491858865	20.41402623188778	27.843020446382067
42	24.143259103540807	27.370711201909824	20.841685139221333	27.644344555328033
43	26.40655187567416	24.38729575326595	21.78906156366106	27.417090807398825
44	25.92061345302324	25.776526619985525	21.47137586511418	26.831484061877052
45	26.2960998604361	24.353951732309298	23.75433693547592	25.595611471778685
46	24.811530269323615	26.690945235953993	21.559861788233068	26.937662706489323
47	25.55280279390019	23.650689984071867	23.84328634545943	26.95322087656851
48	23.86775860856035	25.46166823229836	19.822580133444237	30.84799302569705
49	25.98076361385886	25.747998848022746	19.167716858696917	29.10352067942148
50	23.312523165126496	27.929987754738196	23.143310224246243	25.614178855889065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169279.0
1	145981.5
2	92136.5
3	55575.5
4	43521.5
5	28586.0
6	14747.5
7	10530.0
8	12001.5
9	14462.5
10	16227.5
11	16146.5
12	15669.0
13	14273.5
14	13332.5
15	14311.5
16	15678.5
17	16883.5
18	19488.5
19	23489.5
20	27537.5
21	33858.0
22	41627.0
23	48867.0
24	53920.0
25	57290.5
26	60468.0
27	62102.0
28	61779.0
29	61936.5
30	66485.5
31	78895.5
32	99661.0
33	109915.5
34	112478.0
35	118164.0
36	120261.0
37	123036.0
38	125141.5
39	136998.5
40	163822.0
41	200089.5
42	232350.5
43	256275.0
44	261505.5
45	243030.5
46	240062.5
47	254510.5
48	284913.5
49	345363.0
50	402571.0
51	434422.0
52	450022.0
53	442606.0
54	437867.0
55	445067.0
56	456710.5
57	479965.5
58	495020.0
59	476208.5
60	454534.0
61	474064.0
62	487239.5
63	470653.0
64	442538.5
65	418175.0
66	403511.0
67	390624.5
68	391164.0
69	377414.0
70	327241.5
71	281881.0
72	251632.5
73	224444.5
74	205065.5
75	185643.5
76	159253.0
77	123903.0
78	97175.0
79	87126.0
80	74891.0
81	54734.0
82	40108.0
83	33100.0
84	27813.5
85	24077.5
86	18937.5
87	13522.0
88	7912.0
89	4150.0
90	2409.0
91	1248.0
92	934.5
93	815.5
94	789.0
95	792.0
96	777.0
97	725.5
98	420.0
99	114.5
100	114.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	57361.0
25	47663.0
26	44688.0
27	50677.0
28	54742.0
29	52878.0
30	47533.0
31	47346.0
32	61285.0
33	45328.0
34	49337.0
35	37450.0
36	33934.0
37	42751.0
38	44416.0
39	42090.0
40	49359.0
41	50691.0
42	73715.0
43	104516.0
44	270624.0
45	600860.0
46	812299.0
47	844716.0
48	1176770.0
49	1169103.0
50	1278862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.998896492324665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30090916507469	14.496330754975203
2	10.510985752517696	3.9939426068876593
3	3.642603590150982	2.0761634571554612
4	1.7177547133439366	1.3054177599209709
5	1.08476978174709	1.030471440070729
6	0.7177970469224948	0.8182411078186073
7	0.5308003883507172	0.7059235145452708
8	0.38126053064595244	0.579482348668098
9	0.3290246045730675	0.5625993965140585
>10	2.799816458111366	12.150226283360412
>50	1.1328443751392636	15.632715053254234
>100	0.7714994281732983	25.59297766102525
>500	0.05321459877562244	7.091208053059836
>1k	0.023127019721051103	7.998693277825172
>5k	0.0026944100645884777	3.7729339171318306
>10k+	8.981366881961592E-4	2.192673367787256
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	16657	0.231636961454842	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	11976	0.16654164917951536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	11783	0.16385773649651217	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	10984	0.15274661611454549	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	9634	0.13397313361685464	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9619	0.1337645393668803	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9303	0.12937015383408745	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	8387	0.11663199830232093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	7260	0.10095961698758198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.027729128963255984	0.0	0.0	0.0	0.0
2	0.059157329292723645	0.0	0.0	0.0	0.0
3	0.07598393212398731	0.0	0.0	0.0	0.0
4	0.09669038800477375	0.0	0.0	0.0	0.0
5	0.11748028158554992	0.0	0.0	0.0	0.0
6	0.14694769596525878	0.0	0.0	0.0	0.0
7	0.1665416491795154	0.0	0.0	0.0	0.0
8	0.18612169611044035	0.0	0.0	0.0	0.0
9	0.20472830320815175	0.0	0.0	0.0	0.0
10	0.222639596139282	0.0	0.0	0.0	0.0
11	0.24132964093698311	0.0	0.0	0.0	0.0
12	0.2600474983013475	0.0	0.0	0.0	0.0
13	0.27873754309904863	0.0	0.0	0.0	0.0
14	0.2939232044971808	0.0	0.0	0.0	0.0
15	0.3120709042449486	0.0	0.0	0.0	0.0
16	0.3274095347597286	0.0	0.0	0.0	0.0
17	0.3462525486740776	0.0	0.0	0.0	0.0
18	0.3763874646537043	0.0	0.0	0.0	0.0
19	0.3955642293680123	0.0	0.0	0.0	0.0
20	0.4127523955658981	0.0	0.0	0.0	0.0
21	0.43055243823037537	0.0	0.0	0.0	0.0
22	0.4456685682118494	0.0	0.0	0.0	0.0
23	0.46191110714318495	0.0	0.0	0.0	0.0
24	0.47726364394129656	0.0	0.0	0.0	0.0
25	0.49543915625572765	0.0	0.0	0.0	0.0
26	0.5212770306858829	0.0	0.0	0.0	0.0
27	0.5388545728170542	0.0	0.0	0.0	0.0
28	0.5582399317813365	0.0	0.0	0.0	0.0
29	0.5769995079956958	0.0	0.0	0.0	0.0
30	0.5968298680265899	0.0	0.0	0.0	0.0
31	0.6163403835408569	0.0	0.0	0.0	0.0
32	0.6361568372884194	0.0	0.0	0.0	0.0
33	0.6551111014694213	0.0	0.0	0.0	0.0
34	0.6720489545673379	0.0	0.0	0.0	0.0
35	0.6868591463155164	0.0	0.0	0.0	0.0
36	0.6993887075973085	0.0	0.0	0.0	0.0
37	0.7084973231795215	0.0	0.0	0.0	0.0
38	0.716479529811873	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	6710	0.0	178.13292	44
TAAGCCA	4245	0.0	146.66827	44
ACCACTC	600	0.0	146.12578	44
ACATTGG	1295	0.0	117.40912	44
GTTCGAA	120	0.0	110.98161	44
ACGAACA	170	0.0	110.9816	44
TTAAGCC	3120	0.0	105.068924	43
AATCGCA	860	0.0	103.2387	44
ACTTCTA	2645	0.0	101.12123	44
GTGTTAG	2420	0.0	92.46878	43
CGGTCTA	185	0.0	89.98509	44
TAGATTA	35	1.0300628E-6	83.03319	43
CCTATCA	7540	0.0	83.02217	43
GGATATC	55	3.9147766E-5	80.7139	44
AAATTCC	140	0.0	79.272575	44
TATTCGT	15	0.0014148144	78.464096	42
AGGTACT	310	0.0	75.18108	44
GATTCAA	45	0.0017859846	73.98774	44
TGCACCG	1195	0.0	72.95803	43
TAACGAA	60	0.0	71.92542	42
>>END_MODULE