FastQCFastQC Report
Tue 24 May 2016
ERR840955.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840955.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12445200
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA421470.33866068845820074No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA272820.2192170475363996No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148830.11958827499758942No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA128460.10322051875421849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT172250.0228.8999844
TAAGCCA60550.0172.6298744
ACATTGG22900.0153.8528744
AATCACG606.148184E-10146.1921244
ACTTCTA53850.0144.9704744
AATCGCA19600.0132.7663344
ACCACTC25900.0120.7919644
CTCGATA951.2187229E-10107.7205344
CAGGCAC68700.098.0998244
TTACATG3600.097.46142644
ATAGGTC1400.093.9806744
TTAAGCC49400.093.857543
TAATTCC35700.093.7759144
CCTATCA189800.090.3893243
AACGACA10200.081.5417343
CTTTACA24450.078.32789644
ATCATAA26050.074.63936644
ATTAAGC60100.073.97153542
GATATAC806.399584E-573.0960744
CTAATTC20100.071.6419243
Produced by FastQC (version 0.11.5)