##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840955.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12445200
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.35840420403047	39.0	37.0	39.0	35.0	39.0
2	37.801065310320446	39.0	38.0	39.0	35.0	39.0
3	38.081230434223635	39.0	38.0	39.0	35.0	40.0
4	38.73142344036255	39.0	38.0	41.0	35.0	41.0
5	39.29875381673256	41.0	39.0	41.0	35.0	41.0
6	39.29204761675184	40.0	39.0	41.0	35.0	41.0
7	39.28216147591039	40.0	39.0	41.0	35.0	41.0
8	39.222642946678235	40.0	39.0	41.0	35.0	41.0
9	39.1181525407386	40.0	38.0	41.0	35.0	41.0
10	39.02767701603831	40.0	38.0	41.0	35.0	41.0
11	38.920600070709995	40.0	38.0	41.0	35.0	41.0
12	38.88100882267862	40.0	38.0	41.0	35.0	41.0
13	38.768702551987914	40.0	38.0	41.0	35.0	41.0
14	38.77039019059557	40.0	38.0	41.0	35.0	41.0
15	38.74741996914473	40.0	38.0	41.0	35.0	41.0
16	38.69968542088516	40.0	38.0	41.0	35.0	41.0
17	38.7090213094205	40.0	38.0	41.0	35.0	41.0
18	38.722184778067046	40.0	38.0	41.0	35.0	41.0
19	38.823299022916466	40.0	38.0	41.0	35.0	41.0
20	38.78362798508662	40.0	38.0	41.0	35.0	41.0
21	38.73910953620673	40.0	38.0	41.0	35.0	41.0
22	38.653547150708704	40.0	38.0	41.0	35.0	41.0
23	38.50002362356571	40.0	37.0	41.0	35.0	41.0
24	38.2843244785138	40.0	37.0	41.0	34.0	41.0
25	38.24331258055525	40.0	37.0	41.0	34.0	41.0
26	38.17630933686427	40.0	36.0	41.0	34.0	41.0
27	38.0865388357527	40.0	36.0	41.0	34.0	41.0
28	38.001762208669824	40.0	35.0	41.0	33.0	41.0
29	37.83076245480968	40.0	35.0	41.0	33.0	41.0
30	37.73874615016447	39.0	35.0	41.0	33.0	41.0
31	37.60381214182205	39.0	35.0	41.0	33.0	41.0
32	37.47790510056285	39.0	35.0	41.0	33.0	41.0
33	37.37476792775778	39.0	35.0	41.0	33.0	41.0
34	37.27750750094477	39.0	35.0	41.0	33.0	41.0
35	37.13345295997958	38.0	35.0	41.0	33.0	41.0
36	37.07900723220029	38.0	35.0	41.0	33.0	41.0
37	36.958353881151595	38.0	35.0	40.0	33.0	41.0
38	36.882130712072886	37.0	35.0	40.0	33.0	41.0
39	36.81144697374907	37.0	35.0	40.0	33.0	41.0
40	36.70844081518224	37.0	35.0	40.0	33.0	41.0
41	36.6116230466516	37.0	35.0	40.0	33.0	41.0
42	36.51929845794145	36.0	35.0	40.0	33.0	41.0
43	36.38957060835185	36.0	35.0	40.0	32.0	41.0
44	36.23445398138974	36.0	35.0	40.0	32.0	41.0
45	36.07031705609108	36.0	35.0	40.0	31.0	41.0
46	35.824553450365585	35.0	34.0	40.0	31.0	41.0
47	35.69397559173522	35.0	34.0	39.0	31.0	41.0
48	35.7767696563969	35.0	34.0	40.0	31.0	41.0
49	35.760319981206976	35.0	34.0	39.0	31.0	41.0
50	36.54585722529505	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	8.0
20	44.0
21	115.0
22	276.0
23	522.0
24	1325.0
25	2755.0
26	5456.0
27	10994.0
28	22423.0
29	44449.0
30	83123.0
31	150395.0
32	251094.0
33	436542.0
34	858405.0
35	705671.0
36	1116819.0
37	1710661.0
38	1955862.0
39	2421440.0
40	2666818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.13755504130106	19.46416289011024	38.39828206858869
2	25.36194677466011	19.194259634236495	31.398804358306815	24.044989232796578
3	24.331629865329603	18.27979461961238	28.468429595345995	28.920145919712013
4	22.373469289364575	20.034125606659593	31.204247420692315	26.388157683283513
5	21.711671969916114	19.529955324141035	29.3501430270305	29.408229678912353
6	23.754114035933533	21.89763925047408	26.496601099218974	27.85164561437341
7	27.93373348760968	19.19364895702761	21.084755568411918	31.787861986950794
8	25.9925754507762	21.265146401825604	22.93378169896828	29.808496448429917
9	25.994801208498053	20.49869025809147	25.565776363577925	27.940732169832543
10	29.263941117860703	17.54907112782438	23.653071063542573	29.533916690772344
11	27.352553594960305	20.059058914280204	22.53884228457558	30.049545206183907
12	27.548926493748592	22.201555619837368	22.11844727284415	28.131070613569893
13	29.44556937614502	18.817399479317327	22.907040465400318	28.82999067913734
14	25.87010252948928	20.290561823032174	20.758107543470576	33.08122810400797
15	26.400700671744932	22.882492848648475	22.182946035419278	28.533860444187315
16	26.653207662391925	22.276476071095683	20.38307942017806	30.687236846334333
17	27.609881721466913	25.62151672934143	18.161146466107414	28.607455083084243
18	25.778782181081862	22.658060939157266	19.50467650178382	32.05848037797705
19	29.224110500433902	20.26777392086909	20.974544402661266	29.53357117603574
20	28.243081670041466	19.88193841802462	23.493579854080288	28.38140005785363
21	28.171656542281358	25.221756179089127	18.94004917558577	27.66653810304374
22	30.749847330697776	22.358009513708097	20.308616976826407	26.58352617876772
23	32.16554977019253	21.419535242503134	20.653770128242215	25.761144859062128
24	30.03420595892392	21.42773117346447	22.40094172853791	26.1371211390737
25	29.359643574754944	23.133688891993074	19.783116007964825	27.723551525287153
26	29.91839039162347	22.613937369612067	22.262145772003766	25.205526466760702
27	28.967063956768563	24.94767857351202	19.459801209991376	26.625456259728043
28	29.395652879959243	21.553439215517265	20.88235298937218	28.168554915151304
29	29.347472561385807	25.148051676008702	20.641724413174224	24.86275134943127
30	29.784709175833573	24.625291513590266	19.752962533791713	25.83703677678445
31	28.614257411747918	23.2531016947853	21.74132447209433	26.39131642137245
32	26.498727443328946	23.179727152036964	23.938286035301367	26.38325936933273
33	26.135675058859007	20.760419750849998	23.700888067201138	29.40301712308986
34	28.020403557341627	22.42331915880447	23.456496989193173	26.09978029466073
35	25.078683359546122	21.087731720919038	22.87113549924584	30.962449420288994
36	26.295198020633766	21.691146661542763	25.911732432284555	26.101922885538915
37	24.515439574344473	26.512548935852525	22.27393892279026	26.698072567012737
38	26.533227368524003	22.748228188365264	21.482830885272524	29.23571355783821
39	26.664065824753024	23.505999233432988	22.737361842701468	27.092573099112517
40	25.420497769506127	23.676010316138974	24.296710545654264	26.606781368700634
41	26.269452992185805	22.798007646450987	21.506217077311053	29.426322284052155
42	24.664841405809458	23.814965525340735	21.92442200819933	29.595771060650478
43	25.580471408691164	22.044525257846697	22.693938105907765	29.68106522755437
44	25.82418557253182	23.35261298193808	21.309537604463003	29.513663841067096
45	26.262184534020655	21.43447119447862	25.07976012196551	27.223584149535213
46	25.496472108396528	25.232760087263383	22.28153097940502	26.98923682493507
47	26.302988204319703	21.28932977433672	24.683878890050938	27.723803131292634
48	24.113847199158204	21.98185315387623	21.14324965262767	32.761049994337895
49	24.454946101259907	25.280639562562403	20.4455345952815	29.818879740896193
50	19.437367165806577	28.55458696162692	27.535222819218045	24.472823053348456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161082.0
1	120146.5
2	89431.0
3	87336.0
4	64299.0
5	42620.5
6	24216.5
7	18763.0
8	22788.0
9	25268.5
10	25412.0
11	25414.5
12	24911.5
13	22708.5
14	21485.5
15	21604.0
16	21208.0
17	21307.5
18	23117.0
19	24527.5
20	25108.5
21	27199.0
22	31056.5
23	38145.5
24	44412.5
25	51516.5
26	63819.5
27	73910.0
28	74695.5
29	73156.5
30	83056.5
31	116698.5
32	166549.0
33	186229.5
34	195877.0
35	222975.5
36	232116.5
37	229829.0
38	233365.5
39	256840.0
40	300580.0
41	369710.0
42	426140.5
43	438396.5
44	440622.0
45	420485.0
46	420989.0
47	464090.0
48	519922.5
49	617113.0
50	744898.5
51	837073.0
52	869214.0
53	808506.0
54	754676.5
55	756288.0
56	755652.5
57	777378.0
58	802030.5
59	825546.5
60	844431.5
61	847170.0
62	822875.5
63	781280.5
64	741669.5
65	721189.5
66	705147.0
67	686878.5
68	653633.5
69	606993.0
70	568213.0
71	540771.0
72	501994.5
73	449733.0
74	406788.0
75	366615.5
76	321658.0
77	249605.0
78	189644.0
79	164670.5
80	142427.0
81	110128.0
82	82489.0
83	66875.0
84	54024.0
85	42103.0
86	28870.5
87	19124.0
88	12393.0
89	7848.5
90	5536.5
91	4064.0
92	3183.0
93	2457.0
94	1732.5
95	796.5
96	297.5
97	229.5
98	115.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	74808.0
25	57136.0
26	56805.0
27	51313.0
28	60392.0
29	76698.0
30	69124.0
31	79612.0
32	94869.0
33	78256.0
34	78218.0
35	62234.0
36	59497.0
37	74505.0
38	72860.0
39	70216.0
40	85271.0
41	88816.0
42	129996.0
43	202800.0
44	607083.0
45	1150637.0
46	1376494.0
47	1638575.0
48	2047500.0
49	2306703.0
50	1694782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.406020052476006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59123541058375	12.07339283832519
2	12.519503493633795	4.107904507271989
3	4.1071270589502324	2.021448266616129
4	1.7776807362748208	1.1665866322490008
5	1.0491668418425228	0.8606326122830678
6	0.6597909750487988	0.6494726380255973
7	0.45815139558008305	0.5261508688069696
8	0.35396473530791006	0.4645722036264747
9	0.2773484767241678	0.4095166203594334
>10	2.760406220343386	10.812098669418784
>50	1.387019349155434	16.58491469518233
>100	0.9672762400131649	27.225798746794055
>500	0.0572657930749801	6.410277278475041
>1k	0.030113908427360222	9.960710542891324
>5k	0.0026329100264358665	2.8784181111754887
>10k+	0.0013164550132179333	3.8481047684992413
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	42147	0.33866068845820074	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27282	0.2192170475363996	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14883	0.11958827499758942	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	12846	0.10322051875421849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01732394818885996	0.0	0.0	0.0	0.0
2	0.03365956352650018	0.0	0.0	0.0	0.0
3	0.04424195673834089	0.0	0.0	0.0	0.0
4	0.05438241249638415	0.0	0.0	0.0	0.0
5	0.06479606595313855	0.0	0.0	0.0	0.0
6	0.08004692572236685	0.0	0.0	0.0	0.0
7	0.09276668916530068	0.0	0.0	0.0	0.0
8	0.1043534856812265	0.0	0.0	0.0	0.0
9	0.11500016070452865	0.0	0.0	0.0	0.0
10	0.12542184938771575	0.0	0.0	0.0	0.0
11	0.13596406646739306	0.0	0.0	0.0	0.0
12	0.14702053803876194	0.0	0.0	0.0	0.0
13	0.15683154951306527	0.0	0.0	0.0	0.0
14	0.16714074502619483	0.0	0.0	0.0	0.0
15	0.17906502105229324	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
16	0.18896442001735608	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
17	0.20376530710635427	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
18	0.23410632211615723	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
19	0.24496191302670908	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
20	0.25686208337350946	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
21	0.2690756275511844	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
22	0.27994728891460163	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
23	0.2922652910359014	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
24	0.30353067849452	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
25	0.3158808215215505	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
26	0.3322807186706521	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
27	0.34474335486774016	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
28	0.3581300421045865	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
29	0.37066499533956865	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
30	0.3843007745958281	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
31	0.39767139137980906	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
32	0.4116205444669431	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
33	0.42340018641725324	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
34	0.4342959534599685	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
35	0.4431266673094848	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
36	0.45272072767010574	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
37	0.4597113746665381	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
38	0.46464500369620415	1.6070452865361748E-5	0.0	0.0	8.035226432680874E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	17225	0.0	228.89998	44
TAAGCCA	6055	0.0	172.62987	44
ACATTGG	2290	0.0	153.85287	44
AATCACG	60	6.148184E-10	146.19212	44
ACTTCTA	5385	0.0	144.97047	44
AATCGCA	1960	0.0	132.76633	44
ACCACTC	2590	0.0	120.79196	44
CTCGATA	95	1.2187229E-10	107.72053	44
CAGGCAC	6870	0.0	98.09982	44
TTACATG	360	0.0	97.461426	44
ATAGGTC	140	0.0	93.98067	44
TTAAGCC	4940	0.0	93.8575	43
TAATTCC	3570	0.0	93.77591	44
CCTATCA	18980	0.0	90.38932	43
AACGACA	1020	0.0	81.54173	43
CTTTACA	2445	0.0	78.327896	44
ATCATAA	2605	0.0	74.639366	44
ATTAAGC	6010	0.0	73.971535	42
GATATAC	80	6.399584E-5	73.09607	44
CTAATTC	2010	0.0	71.64192	43
>>END_MODULE