##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840954.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1696724
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.17486992580997	39.0	35.0	39.0	31.0	39.0
2	36.946346606755135	39.0	35.0	39.0	33.0	39.0
3	37.10511845179298	39.0	35.0	39.0	33.0	39.0
4	37.488249709440076	39.0	35.0	39.0	34.0	41.0
5	38.34871493536957	40.0	36.0	41.0	34.0	41.0
6	38.257397196008306	40.0	36.0	41.0	34.0	41.0
7	38.234898545668	40.0	36.0	41.0	34.0	41.0
8	38.18309872436531	40.0	36.0	41.0	34.0	41.0
9	38.15090904590257	40.0	35.0	41.0	34.0	41.0
10	38.07517015142121	40.0	35.0	41.0	34.0	41.0
11	37.883820232400794	40.0	35.0	41.0	33.0	41.0
12	37.98470817881989	40.0	35.0	41.0	34.0	41.0
13	37.80572208561911	39.0	35.0	41.0	33.0	41.0
14	37.795508874749224	39.0	35.0	41.0	33.0	41.0
15	37.786387768429044	39.0	35.0	41.0	34.0	41.0
16	37.73648866875225	39.0	35.0	41.0	33.0	41.0
17	37.83845103858966	39.0	35.0	41.0	34.0	41.0
18	37.892191069378406	39.0	35.0	41.0	34.0	41.0
19	37.931882262524724	40.0	35.0	41.0	34.0	41.0
20	37.999679382150546	40.0	35.0	41.0	34.0	41.0
21	38.023187035722955	40.0	35.0	41.0	34.0	41.0
22	37.94212081634962	40.0	35.0	41.0	34.0	41.0
23	37.82504874098557	40.0	35.0	41.0	34.0	41.0
24	37.66700535856156	39.0	35.0	41.0	33.0	41.0
25	37.69379408719223	39.0	35.0	41.0	33.0	41.0
26	37.714815567553906	39.0	35.0	41.0	33.0	41.0
27	37.66975829789676	39.0	35.0	41.0	33.0	41.0
28	37.56916245026163	39.0	35.0	41.0	33.0	41.0
29	37.49126627552761	39.0	35.0	41.0	33.0	41.0
30	37.4465855565861	39.0	35.0	41.0	33.0	41.0
31	37.321797231432924	39.0	35.0	41.0	33.0	41.0
32	37.26565839605985	38.0	35.0	41.0	33.0	41.0
33	37.093308859926644	38.0	35.0	41.0	33.0	41.0
34	36.969238704735545	38.0	35.0	40.0	33.0	41.0
35	36.891208999400575	37.0	35.0	40.0	33.0	41.0
36	36.87685417263327	37.0	35.0	40.0	33.0	41.0
37	36.71827719761789	37.0	35.0	40.0	33.0	41.0
38	36.631457519316264	36.0	35.0	40.0	33.0	41.0
39	36.51471282016905	36.0	35.0	40.0	33.0	41.0
40	36.39697308854158	36.0	35.0	40.0	33.0	41.0
41	36.35484195131006	36.0	35.0	40.0	33.0	41.0
42	36.285999869820785	36.0	35.0	39.0	33.0	41.0
43	36.125477563472145	35.0	35.0	39.0	33.0	41.0
44	35.95052000029141	35.0	35.0	39.0	33.0	41.0
45	35.70484488648887	35.0	35.0	39.0	32.0	41.0
46	35.48548110495693	35.0	34.0	39.0	31.0	40.0
47	35.29763317410553	35.0	34.0	38.0	31.0	40.0
48	35.10843253968254	35.0	34.0	38.0	31.0	40.0
49	35.39731576796528	35.0	34.0	37.0	32.0	40.0
50	35.01458740164346	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	1.0
21	21.0
22	71.0
23	151.0
24	326.0
25	766.0
26	1407.0
27	2800.0
28	5350.0
29	10009.0
30	17547.0
31	32101.0
32	53713.0
33	99257.0
34	214648.0
35	96547.0
36	142924.0
37	197073.0
38	257203.0
39	341580.0
40	223224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.26789743057798	15.08247658428831	36.6496259851337
2	26.561302840061202	24.76324965050297	29.269875359811024	19.405572149624806
3	27.93447844198585	18.53931458504742	24.621446976644403	28.904759996322326
4	22.716658690511835	24.321751799349805	27.166704779327695	25.79488473081067
5	22.114321480688666	25.606993241092834	27.58439204019039	24.694293238028106
6	21.938394223220747	31.19381820496439	23.17577873596413	23.692008835850732
7	28.56357309733345	25.942050681195056	20.687336302191753	24.80703991927974
8	25.119465511185084	26.459459523175248	22.838187000360698	25.58288796527897
9	23.0213045845995	28.673255049141762	25.414858279838086	22.890582086420654
10	28.254506920394835	22.97539258005427	21.499961101510912	27.270139398039987
11	22.768700153943715	24.867745137099494	26.01548631362555	26.348068395331236
12	24.80509499482532	25.817634453216904	25.43536839226651	23.941902159691264
13	23.18750721979532	25.867200558252257	25.95590090079471	24.989391321157715
14	19.08342193544737	26.95093603909652	24.2643470593921	29.70129496606401
15	22.67770126431877	28.251972624893618	25.645066610715713	23.425259500071903
16	20.370313616121418	34.25996213880396	20.37202279215712	24.997701452917504
17	22.105009418149326	33.81952515553502	19.987163498600832	24.08830192771482
18	22.03245784228902	28.148891628809398	21.58247304806203	28.236177480839547
19	23.857857848418483	27.497636622102355	23.338975578821305	25.305529950657856
20	23.70255857758834	23.7121063885464	24.180715307852072	28.404619726013188
21	24.46184529717267	28.587206876309878	20.283676072242745	26.667271754274708
22	23.779766184718316	26.29196027167648	23.880725445034077	26.047548098571127
23	26.073892984362807	24.933695757235707	23.378993872898597	25.613417385502885
24	23.708334413846917	27.858508514054144	25.21747791626688	23.21567915583206
25	22.162781109534087	28.16533304267676	23.474882295934204	26.197003551854948
26	25.219704343068365	27.648625629759515	23.598138778643516	23.5335312485286
27	22.617897339985362	28.907927905519493	22.577956462051382	25.896218292443763
28	23.162291771226307	26.52967252520838	21.852704782808114	28.4553309207572
29	22.25189049874449	31.184821040018416	22.366773030451707	24.196515430785386
30	23.014409466479957	30.388091730772725	21.686944186807793	24.910554615939525
31	23.13818002989624	29.55266412499939	22.55108897296192	24.75806687214245
32	21.952873192180338	27.462963234682746	24.732770384282425	25.851393188854487
33	22.019609470839445	27.23053887612951	24.159864227934733	26.58998742509631
34	22.004793615102358	29.535464103482784	24.37794195772229	24.081800323692566
35	22.199784456653738	28.456007727260022	21.87889197054448	27.46531584554176
36	21.84169033031774	29.160356879680084	25.48948036057352	23.508472429428657
37	20.851240823879245	30.74569703438802	23.251455306599414	25.15160683513332
38	22.67865184935453	28.75129257314517	22.10526514417694	26.464790433323355
39	22.68480984320238	29.371696492827937	22.57692215264401	25.36657151132568
40	22.279189459387485	30.4580026045192	23.655110304375377	23.607697631717933
41	23.18382421183677	30.369607030502422	20.534456438599914	25.912112319060892
42	21.610587565822673	31.172315392016664	21.782656132891066	25.434440909269597
43	23.372767191308053	27.259251347207698	23.635695760968815	25.732285700515433
44	24.937098608321197	29.310757048631604	20.281567756339314	25.47057658670789
45	22.347434979061052	26.91240081551686	27.321812320916905	23.418351884505178
46	23.201241110368578	32.01248543947691	21.87761801364359	22.908655436510916
47	23.047823658623194	28.762091528925986	23.72928536632805	24.46079944612277
48	22.370308366402117	28.7397693452381	20.284804894179896	28.605117394179896
49	21.383078903628995	31.22160178453457	20.847137039914585	26.54818227192185
50	20.567616748549177	32.26622180101747	27.493936257850738	19.672225192582616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29436.0
1	22631.5
2	15651.5
3	13997.0
4	10740.5
5	7597.0
6	5304.0
7	5643.0
8	7316.5
9	6365.5
10	4257.0
11	3964.0
12	4503.0
13	4614.5
14	5026.0
15	5482.0
16	5228.5
17	4820.0
18	5312.0
19	6520.5
20	7706.0
21	8859.0
22	10102.5
23	14502.5
24	19758.0
25	20411.0
26	21100.5
27	24553.5
28	25357.0
29	25350.0
30	29193.0
31	34812.0
32	42891.5
33	51790.0
34	58565.0
35	61670.0
36	60004.0
37	56950.0
38	55777.0
39	60251.0
40	70437.5
41	84276.0
42	93038.5
43	95517.5
44	96942.5
45	94766.5
46	90859.5
47	87131.5
48	89703.0
49	104362.0
50	117885.5
51	116494.5
52	109137.5
53	101699.5
54	97241.0
55	90288.0
56	83764.5
57	82824.0
58	80154.5
59	77946.0
60	77669.0
61	77212.0
62	75030.0
63	72922.0
64	71205.5
65	70299.0
66	64298.5
67	55714.5
68	54955.5
69	56531.5
70	50074.0
71	41220.0
72	35016.5
73	27996.0
74	22063.5
75	19587.0
76	16944.5
77	11529.0
78	8488.0
79	8238.5
80	6956.0
81	4974.5
82	3336.5
83	2710.0
84	2278.5
85	1512.5
86	1237.0
87	1357.0
88	1041.5
89	550.0
90	308.0
91	175.5
92	58.0
93	27.5
94	18.5
95	9.5
96	1.5
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	8592.0
25	10309.0
26	5351.0
27	6756.0
28	6630.0
29	9476.0
30	6594.0
31	7344.0
32	9417.0
33	7435.0
34	8935.0
35	7216.0
36	6270.0
37	6547.0
38	8578.0
39	7855.0
40	11084.0
41	10628.0
42	16309.0
43	25495.0
44	58063.0
45	106593.0
46	224421.0
47	153146.0
48	348583.0
49	183310.0
50	435787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.471179213036939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33110206011413	9.269964948942226
2	10.413535447523254	2.597381336147506
3	3.8439598160361315	1.4381613526049728
4	1.909297095400244	0.9524474499066932
5	1.1299151067995434	0.7045686896207439
6	0.7630705217612558	0.5709833537442135
7	0.5377835632436323	0.46947566365258453
8	0.44505523132539376	0.44402908396468777
9	0.3375108593583173	0.37882425720633134
>10	2.957013882261363	8.49554355968007
>50	1.4714060753194416	13.510470400613487
>100	1.734093048505792	40.38208754226429
>500	0.07599372572816962	6.332785806573256
>1k	0.047271687657680306	11.389940110500294
>5k	0.0023935031725407755	1.9790422048775982
>10k+	5.983757931351939E-4	1.084294239701037
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	14530	0.8563561309912513	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8283	0.48817603805922477	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	6605	0.38927957640724126	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	6544	0.3856844130217996	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5088	0.2998719886086364	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4750	0.2799512472270092	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	4399	0.25926432348455025	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4285	0.252545493551102	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	4202	0.2476537138627143	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	4060	0.23928464499824367	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	3901	0.2299136453542238	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	3712	0.2187745325698228	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	3493	0.2058673066450407	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	3463	0.20409919350465955	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	3441	0.20280257720171344	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG	3224	0.19001322548629004	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	3198	0.18848086076462642	No Hit
AATTCGTGGAGAAAGAAATGGCTC	3116	0.18364801818091805	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	3075	0.18123159688906387	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2924	0.17233209408247893	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	2584	0.15229347849149302	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2429	0.14315822726619062	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA	2415	0.14233310780067943	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	2296	0.13531959234383437	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG	2165	0.12759883163083685	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	2152	0.126832649270005	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2060	0.12141043563950295	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	2015	0.11875826592893128	No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGG	1989	0.11722590120726765	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	1986	0.11704908989322955	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	1982	0.11681334147451207	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	1911	0.11262880704227676	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	1902	0.11209837310016244	No Hit
ATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAAAA	1902	0.11209837310016244	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	1893	0.1115679391580481	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	1877	0.11062494548317817	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	1874	0.11044813416914007	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1859	0.10956407759894951	No Hit
CAGAGTGTGGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	1852	0.10915151786619391	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1811	0.10673509657433973	No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC	1787	0.10532060606203485	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1718	0.10125394583915827	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1717	0.10119500873447891	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.023869527395144998	0.0	0.0	0.0	0.0
2	0.041845344322352956	0.0	0.0	0.0	0.0
3	0.0526897715833571	0.0	0.0	0.0	0.0
4	0.06359313594904062	0.0	0.0	0.0	0.0
5	0.07532161978023533	0.0	0.0	0.0	0.0
6	0.09412255617295447	0.0	0.0	0.0	0.0
7	0.10932832918023203	0.0	0.0	0.0	0.0
8	0.12241236641905225	0.0	0.0	0.0	0.0
9	0.1334336049940945	0.0	0.0	0.0	0.0
10	0.14469059198785425	0.0	0.0	0.0	0.0
11	0.15753888080795697	0.0	0.0	0.0	0.0
12	0.16832437096428177	0.0	0.0	0.0	0.0
13	0.17869730138785095	0.0	0.0	0.0	0.0
14	0.19036684811436627	0.0	0.0	0.0	0.0
15	0.20297938851575154	0.0	0.0	0.0	0.0
16	0.2135880673580382	0.0	0.0	0.0	0.0
17	0.22944214851678882	0.0	0.0	0.0	0.0
18	0.25749621034416914	0.0	0.0	0.0	0.0
19	0.2692836312800432	0.0	0.0	0.0	0.0
20	0.2790082535521393	0.0	0.0	0.0	0.0
21	0.29044205185993716	0.0	0.0	0.0	0.0
22	0.3001077370273539	0.0	0.0	0.0	0.0
23	0.3102449190322056	0.0	0.0	0.0	0.0
24	0.3223270254914765	0.0	0.0	0.0	0.0
25	0.3338197609039537	0.0	0.0	0.0	0.0
26	0.3470806094568121	0.0	0.0	0.0	0.0
27	0.35821972224121307	0.0	0.0	0.0	0.0
28	0.36871052687414096	0.0	0.0	0.0	0.0
29	0.3843288596141741	0.0	0.0	0.0	0.0
30	0.3959394692360101	0.0	0.0	0.0	0.0
31	0.409612877521624	0.0	0.0	0.0	0.0
32	0.4218128581902537	0.0	0.0	0.0	0.0
33	0.4335413420214484	0.0	0.0	0.0	0.0
34	0.44361958692162073	0.0	0.0	0.0	0.0
35	0.45263696393756436	0.0	0.0	0.0	0.0
36	0.46583887538574337	0.0	0.0	0.0	0.0
37	0.472145145586436	0.0	0.0	0.0	0.0
38	0.4802784660321891	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCTT	20	1.3282806E-6	159.41692	44
CACGAGT	30	3.3651304E-10	159.41692	44
ATGTCGC	10	0.005339745	159.41692	44
TCGTAGG	35	5.456968E-12	159.41692	44
GCTAGAC	25	2.1051164E-8	159.41692	44
GGACTCG	45	0.0	141.70393	44
GTCGAAC	30	6.2522304E-8	132.84743	44
CGCGTCC	25	4.0324812E-6	127.53354	44
TGAACGT	20	2.650322E-4	119.56269	44
GACGATC	75	0.0	116.90574	44
TAACGAA	25	1.8170795E-7	111.09939	43
CTATCAT	2475	0.0	107.2441	44
TAGGCGT	30	9.981861E-6	106.27795	44
ACCCGAT	30	9.981861E-6	106.27795	44
AACGCTT	60	0.0	101.84111	43
CACCGTC	55	1.9645086E-10	101.44713	44
GTGCACC	150	0.0	100.96405	44
TACGAGA	45	5.456968E-12	98.755005	43
ACGCCAA	65	7.2759576E-12	98.10272	44
TACGTCG	75	0.0	96.28613	43
>>END_MODULE