FastQCFastQC Report
Tue 24 May 2016
ERR840953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6416901
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA138350.2156025159185096No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG84980.13243152730578203No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66120.10304039286253598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA151.3456487E-5252.5671744
CTATCAT59450.0195.8510744
ATACGTT301.9554136E-9123.8114143
ACTTCTA24050.0108.1680644
ATTACGT355.7098077E-9106.1240743
ACGAACA1057.2759576E-1296.2160744
CCTATCA61000.093.7719243
ACATTGG10100.090.0239444
GACATTG5950.078.032443
AATACGA1050.076.64516443
TATTTCG1005.7916623E-975.7701544
GATGCGT1005.916263E-875.7701544
AATGTCG250.001757772374.2868543
CGAGATA1402.5465852E-1172.16205644
TGAGCAT4450.070.9458444
CCCTATC84800.069.9267942
TCGTGCG14950.067.90993543
TCGCAAA9450.067.4739443
CTCTATC4500.067.3512444
CGATATT557.2759576E-1267.1508342
Produced by FastQC (version 0.11.5)