##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840953.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6416901
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.01640012834856	39.0	37.0	39.0	33.0	39.0
2	37.641438600969536	39.0	37.0	39.0	35.0	39.0
3	37.77945880729654	39.0	37.0	39.0	35.0	40.0
4	38.2622261119503	39.0	38.0	40.0	35.0	41.0
5	38.84050992839067	40.0	38.0	41.0	35.0	41.0
6	38.823234455385865	40.0	38.0	41.0	35.0	41.0
7	38.7706889976953	40.0	38.0	41.0	35.0	41.0
8	38.66237347280253	40.0	38.0	41.0	35.0	41.0
9	38.48820201527186	40.0	38.0	41.0	35.0	41.0
10	38.377997104833	40.0	38.0	41.0	34.0	41.0
11	38.247359434094435	40.0	37.0	41.0	34.0	41.0
12	38.17391260984079	40.0	37.0	41.0	34.0	41.0
13	38.00714051845276	39.0	37.0	41.0	33.0	41.0
14	37.975631071758784	39.0	37.0	41.0	33.0	41.0
15	37.92473719011716	39.0	37.0	40.0	33.0	41.0
16	37.882378581187396	39.0	36.0	40.0	33.0	41.0
17	37.935597105207016	39.0	36.0	41.0	33.0	41.0
18	38.01561719590188	39.0	36.0	41.0	33.0	41.0
19	38.11495439932765	40.0	36.0	41.0	34.0	41.0
20	38.05850565561164	40.0	36.0	41.0	34.0	41.0
21	38.029198673939334	40.0	36.0	41.0	34.0	41.0
22	37.839899664962886	39.0	35.0	41.0	33.0	41.0
23	37.709837349836	39.0	35.0	41.0	33.0	41.0
24	37.46882490473205	39.0	35.0	41.0	33.0	41.0
25	37.34995664889182	39.0	35.0	41.0	33.0	41.0
26	37.236221340771145	39.0	35.0	41.0	33.0	41.0
27	37.127760561168486	38.0	35.0	41.0	33.0	41.0
28	37.019995221054955	38.0	35.0	40.0	33.0	41.0
29	36.87928008511695	38.0	35.0	40.0	33.0	41.0
30	36.760718860259416	38.0	35.0	40.0	32.0	41.0
31	36.56327746542118	37.0	35.0	40.0	32.0	41.0
32	36.385883140661655	37.0	35.0	40.0	31.0	41.0
33	36.29233216005231	37.0	35.0	40.0	31.0	41.0
34	36.228642731901225	36.0	35.0	40.0	32.0	41.0
35	36.07336039987113	36.0	34.0	40.0	31.0	41.0
36	35.97462569903665	36.0	34.0	40.0	31.0	41.0
37	35.91857519958289	35.0	34.0	40.0	31.0	41.0
38	35.867148274965956	35.0	34.0	40.0	31.0	41.0
39	35.79540636746807	35.0	34.0	39.0	31.0	41.0
40	35.7388807571434	35.0	34.0	39.0	31.0	41.0
41	35.66539871407883	35.0	34.0	39.0	31.0	41.0
42	35.55289293024693	35.0	34.0	39.0	31.0	41.0
43	35.41618999099739	35.0	34.0	39.0	31.0	41.0
44	35.22300231554515	35.0	34.0	39.0	31.0	41.0
45	35.100341218444846	35.0	34.0	39.0	30.0	41.0
46	34.810347313232946	35.0	33.0	38.0	30.0	40.0
47	34.65980340327665	35.0	33.0	37.0	30.0	40.0
48	34.665106679177576	35.0	33.0	37.0	29.0	40.0
49	34.6683260330641	35.0	33.0	37.0	30.0	40.0
50	35.07874651101247	35.0	34.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	54.0
22	139.0
23	307.0
24	818.0
25	1863.0
26	3987.0
27	9004.0
28	19219.0
29	37916.0
30	69807.0
31	121201.0
32	199688.0
33	332805.0
34	547113.0
35	588878.0
36	845013.0
37	1014842.0
38	963478.0
39	1013333.0
40	647421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	44.46926951187185	15.972258259867186	39.55847222826096
2	27.493193365457874	18.06562077239465	31.496434182169867	22.94475167997761
3	23.928341733805773	16.13903658479381	28.301028798792437	31.631592882607972
4	20.8354936440503	19.048525136978114	33.70179779928037	26.414183419691216
5	21.990396922127985	20.374539049301212	29.8460425055646	27.7890215230062
6	22.69681268263294	22.514824523551166	27.878815646368864	26.909547147447032
7	28.56399062413461	19.674730839699723	21.90083655646238	29.86044197970329
8	28.467838291412008	20.768529855766825	22.74551220285306	28.01811964996811
9	26.634040325696155	20.602312549313133	25.105498744643246	27.65814838034746
10	29.85523697498216	18.482925013180036	23.144645678653916	28.517192333183882
11	30.242900739780776	19.390855492394227	23.273492921271497	27.0927508465535
12	28.707206173197935	20.227053526304985	22.813909705011813	28.251830595485266
13	29.60012940826109	18.973068152368253	24.752368783623123	26.67443365574753
14	26.207946795501442	20.731642891171298	22.23495422478857	30.82545608853869
15	25.96338949284086	23.223094761786104	22.29119632670038	28.52231941867266
16	27.14495673222947	24.128609744797373	20.041512250227953	28.684921272745207
17	27.97155511671444	24.302821564490397	19.12055367536448	28.605069643430685
18	26.297538328859993	21.486493246506374	19.93931338507482	32.27665503955882
19	29.27171542774308	20.122080736480115	20.72869754418839	29.877506291588418
20	28.828604337202645	20.4282098165454	23.827249321752042	26.915936524499912
21	30.073878340962406	23.696656688329774	19.582583555520024	26.6468814151878
22	30.660298483644986	21.53787318831941	20.97725989539187	26.82456843264373
23	31.667981164116448	21.445710320293237	21.59841643185706	25.287892083733254
24	31.175157603335318	21.8578095563575	22.359609412705602	24.607423427601578
25	29.5332996489518	22.983969353702648	21.198848911103692	26.283882086241857
26	31.938022536512584	22.961761994937326	20.96266633720212	24.13754913134797
27	29.400753494163652	24.27559905675881	19.16859364428451	27.155053804793027
28	29.142093114038538	22.52475563810097	20.881785638759034	27.451365609101465
29	29.816588263133216	24.084399182823287	21.12635057536354	24.97266197867996
30	31.01980227155211	24.058513340510014	20.006270164204572	24.915414223733297
31	29.745704001517215	22.656092669870105	20.85197534138907	26.746227987223612
32	27.486046128841814	23.02350137772815	24.178825072385347	25.311627421044697
33	25.73734586490971	21.909817014912456	22.7864868928909	29.566350227286932
34	29.14631763689916	21.721423878597914	24.43894171284666	24.693316771656267
35	26.718435195503726	21.758781537605472	22.575139620254465	28.94764364663634
36	27.663310974112065	21.91794554808137	24.38632011909237	26.03242335871419
37	26.340724811718925	24.745478676274008	23.37317877730746	25.540617734699612
38	27.372421641622267	22.239860401929207	21.732396786121093	28.65532117032743
39	26.79829879586157	23.29850550932551	22.955283154109438	26.947912540703484
40	25.89670609307043	23.03866576593738	23.848850688406625	27.21577745258556
41	25.250524357400312	23.564834587554216	22.111967601631328	29.072673453414144
42	25.502985156767334	23.993488221812324	22.573661383983655	27.92986523743669
43	25.2220271976576	23.216681718951783	21.88002441921669	29.681266664173933
44	25.498992834965286	24.407179765675185	21.850735833271955	28.243091566087582
45	27.897492001845848	21.58891887874086	23.667993756329615	26.845595363083678
46	26.07529284161557	24.05333806498497	23.644863450752663	26.226505642646792
47	26.226949267683786	22.82029272007994	23.906918038429218	27.045839973807055
48	24.674277648487628	23.686992585510495	21.18961293167074	30.449116834331136
49	25.3667001401203	25.851000375052248	21.161725552470493	27.62057393235696
50	21.325590568873487	28.07457820075597	26.22130791469644	24.378523315674105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65730.0
1	52995.0
2	43065.0
3	42733.5
4	34654.5
5	24137.0
6	15512.0
7	13176.5
8	14319.0
9	15180.5
10	15332.0
11	14189.5
12	12818.5
13	12057.5
14	12561.5
15	13657.5
16	14593.0
17	15657.5
18	15851.5
19	16866.0
20	19000.0
21	21119.5
22	22910.5
23	25894.5
24	29681.5
25	33589.0
26	39501.5
27	45641.5
28	49154.5
29	54767.0
30	66001.5
31	81586.5
32	101318.0
33	113820.5
34	122827.5
35	130148.0
36	131891.5
37	136101.5
38	141727.0
39	150290.0
40	160749.5
41	184671.0
42	208339.5
43	204528.0
44	197681.0
45	202161.0
46	216109.5
47	232260.0
48	244979.0
49	277277.5
50	324449.0
51	361976.0
52	389641.0
53	386243.0
54	379436.5
55	390942.0
56	397560.5
57	404822.5
58	409662.5
59	414138.0
60	425225.5
61	435006.5
62	436019.5
63	431870.0
64	413149.5
65	391778.0
66	380250.5
67	371399.0
68	352091.0
69	323283.5
70	300370.5
71	289320.0
72	277602.0
73	251161.5
74	227397.0
75	208142.5
76	182085.0
77	142910.5
78	105614.0
79	82872.0
80	66025.5
81	52734.5
82	43031.5
83	34678.5
84	26270.5
85	19848.0
86	14840.5
87	11553.5
88	7491.0
89	3665.0
90	1830.0
91	1075.0
92	801.5
93	685.0
94	438.5
95	147.5
96	61.0
97	60.0
98	34.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	47975.0
25	39464.0
26	31603.0
27	37063.0
28	35949.0
29	40001.0
30	47274.0
31	50014.0
32	55755.0
33	48512.0
34	45578.0
35	42457.0
36	37790.0
37	43059.0
38	45552.0
39	41644.0
40	46925.0
41	47416.0
42	70038.0
43	104938.0
44	291702.0
45	567375.0
46	722964.0
47	779888.0
48	1045584.0
49	1049016.0
50	1001365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.192556151178852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.28274378331712	15.807286995129779
2	9.888786648860872	3.993597593483019
3	3.155173969658267	1.911330826471873
4	1.4814892479195862	1.196602193039359
5	0.8614038334988049	0.8696972638382667
6	0.5598681919226665	0.6783101941594453
7	0.40929323624866004	0.5785273658674145
8	0.3170959639462736	0.5122382445837852
9	0.2598959521382238	0.47231672463136576
>10	2.6441888660636033	12.754549803376298
>50	1.1897768740588919	17.451862343476403
>100	0.890060879655132	29.94176506016611
>500	0.04078062624585842	5.733806539641022
>1k	0.01873063647338846	6.712341590772604
>5k	4.741933284402142E-4	0.7234045023043306
>10k+	2.370966642201071E-4	0.6623627590589287
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13835	0.2156025159185096	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	8498	0.13243152730578203	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6612	0.10304039286253598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.013433275657517547	0.0	0.0	0.0	0.0
2	0.028705445198546776	0.0	0.0	0.0	0.0
3	0.03858560386080446	0.0	0.0	0.0	0.0
4	0.05010206640245814	0.0	0.0	0.0	0.0
5	0.06057441123059246	0.0	0.0	0.0	0.0
6	0.07586216461809213	0.0	0.0	0.0	0.0
7	0.08658385098975346	0.0	0.0	0.0	0.0
8	0.09749254351906006	0.0	0.0	0.0	0.0
9	0.10826098143013271	0.0	0.0	0.0	0.0
10	0.11786063085592251	0.0	0.0	0.0	0.0
11	0.12691484565524697	0.0	0.0	0.0	0.0
12	0.13822871819278496	0.0	0.0	0.0	0.0
13	0.14863872763503755	0.0	0.0	0.0	0.0
14	0.1589240663055266	0.0	0.0	0.0	0.0
15	0.16989509422071494	0.0	0.0	0.0	1.558384647043799E-5
16	0.18052327751355365	0.0	0.0	0.0	1.558384647043799E-5
17	0.1930838577687267	0.0	0.0	0.0	1.558384647043799E-5
18	0.21748816134143256	0.0	0.0	0.0	1.558384647043799E-5
19	0.22757091000780594	0.0	0.0	0.0	1.558384647043799E-5
20	0.23921204332122312	0.0	0.0	0.0	1.558384647043799E-5
21	0.25041682893346806	0.0	0.0	0.0	1.558384647043799E-5
22	0.2605307452927823	0.0	0.0	0.0	1.558384647043799E-5
23	0.27192253706267244	0.0	0.0	0.0	1.558384647043799E-5
24	0.28376626038020536	0.0	0.0	0.0	1.558384647043799E-5
25	0.2964515114071419	0.0	0.0	0.0	1.558384647043799E-5
26	0.31134966863288055	0.0	0.0	0.0	1.558384647043799E-5
27	0.32327131118276564	0.0	0.0	0.0	1.558384647043799E-5
28	0.3356916368197047	0.0	0.0	1.558384647043799E-5	1.558384647043799E-5
29	0.3487976517013431	0.0	0.0	1.558384647043799E-5	1.558384647043799E-5
30	0.3617634119647475	0.0	0.0	1.558384647043799E-5	1.558384647043799E-5
31	0.37608496687108	0.0	0.0	1.558384647043799E-5	1.558384647043799E-5
32	0.39017276408035595	0.0	0.0	1.558384647043799E-5	1.558384647043799E-5
33	0.4032164435761125	0.0	0.0	1.558384647043799E-5	1.558384647043799E-5
34	0.4154497630554063	1.558384647043799E-5	0.0	1.558384647043799E-5	1.558384647043799E-5
35	0.4254390086429571	1.558384647043799E-5	0.0	1.558384647043799E-5	1.558384647043799E-5
36	0.4346334780605155	1.558384647043799E-5	0.0	1.558384647043799E-5	1.558384647043799E-5
37	0.4414903705075082	1.558384647043799E-5	0.0	1.558384647043799E-5	1.558384647043799E-5
38	0.44585384751923085	1.558384647043799E-5	0.0	1.558384647043799E-5	1.558384647043799E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGA	15	1.3456487E-5	252.56717	44
CTATCAT	5945	0.0	195.85107	44
ATACGTT	30	1.9554136E-9	123.81141	43
ACTTCTA	2405	0.0	108.16806	44
ATTACGT	35	5.7098077E-9	106.12407	43
ACGAACA	105	7.2759576E-12	96.21607	44
CCTATCA	6100	0.0	93.77192	43
ACATTGG	1010	0.0	90.02394	44
GACATTG	595	0.0	78.0324	43
AATACGA	105	0.0	76.645164	43
TATTTCG	100	5.7916623E-9	75.77015	44
GATGCGT	100	5.916263E-8	75.77015	44
AATGTCG	25	0.0017577723	74.28685	43
CGAGATA	140	2.5465852E-11	72.162056	44
TGAGCAT	445	0.0	70.94584	44
CCCTATC	8480	0.0	69.92679	42
TCGTGCG	1495	0.0	67.909935	43
TCGCAAA	945	0.0	67.47394	43
CTCTATC	450	0.0	67.35124	44
CGATATT	55	7.2759576E-12	67.15083	42
>>END_MODULE