FastQCFastQC Report
Tue 24 May 2016
ERR840952.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840952.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285186
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA33880.26361942940554906No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA31300.24354451417927056No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT28910.22494798418283424No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG26270.20440621046292134No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA23840.18549844147072875No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC23080.1795849005513599No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22440.17460507661925978No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG20320.1581094098441782No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT18320.14254746005636537No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG17470.13593363139654493No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC16930.13173190495383547No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC16100.12527369579189315No Hit
CTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACCCTGCGT16090.1251958860429541No Hit
AGAGTGCAGTGCTACGAGCGGGATGGCACATGCAGCGCAAGTAGGTCTAC15990.12441778855356346No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG15540.12091634985130557No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15140.11780395989374301No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC14930.11616995516602266No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA14890.1158587161702664No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC14400.11204603847225227No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG13860.1078443120295428No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC13440.10457630257410212No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG13400.10426506357834586No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG13350.10387601483365053No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT13260.10317572709319896No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG13200.10270886859956457No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG13110.102008580859113No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA13090.10185296136123487No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT12870.10014114688457545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT800.0398.7200644
TATAAAT103.4293378E-4398.7200644
ACCTTAA103.4293378E-4398.7200644
TTTGTAC700.0398.7244
GAATATG152.169636E-6398.7244
CCCGTAC400.0348.8800444
TATGTTC400.0348.8800444
GGTTATC351.8189894E-12341.7644
ACGCTTA302.6011548E-10332.2666644
GCGAGAC500.0318.97644
GGTCGAG206.8433983E-6299.0400444
GTTAGTC206.8433983E-6299.0400444
CTATCAT5800.0295.6027844
TTTACTA150.00115523265.8133544
CAAAAAT459.094947E-12265.8133544
TTTACCG301.0363692E-7265.8133544
GAATCAA150.00115523265.8133544
TGCATAA301.0363692E-7265.8133544
CCTATGA150.00115523265.8133544
GGTAGCC301.0363692E-7265.8133544
Produced by FastQC (version 0.11.5)