##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840952.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1285186
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.78699114369437	39.0	37.0	39.0	32.0	39.0
2	37.51387270013834	39.0	37.0	39.0	35.0	39.0
3	37.70813718792455	39.0	37.0	39.0	35.0	40.0
4	38.3786875985266	39.0	38.0	41.0	35.0	41.0
5	38.74208402519169	40.0	38.0	41.0	35.0	41.0
6	38.69363500691728	40.0	38.0	41.0	35.0	41.0
7	38.559116734853944	40.0	38.0	41.0	35.0	41.0
8	38.422628319947464	40.0	37.0	41.0	34.0	41.0
9	38.2169639258442	40.0	37.0	41.0	34.0	41.0
10	37.99673821532448	39.0	37.0	41.0	33.0	41.0
11	37.71470044024756	39.0	36.0	41.0	33.0	41.0
12	37.594806510497314	39.0	35.0	40.0	33.0	41.0
13	37.38778511437255	39.0	35.0	40.0	33.0	41.0
14	37.21390600271089	38.0	35.0	40.0	33.0	41.0
15	37.102091059192986	38.0	35.0	40.0	33.0	41.0
16	36.99362037868448	38.0	35.0	40.0	32.0	41.0
17	36.99831697513045	38.0	35.0	40.0	33.0	41.0
18	36.86114305633581	38.0	35.0	40.0	33.0	41.0
19	36.887384394165515	37.0	35.0	40.0	33.0	41.0
20	36.807616951943146	37.0	35.0	40.0	33.0	41.0
21	36.63836674224587	37.0	35.0	40.0	33.0	41.0
22	36.43300113757853	36.0	35.0	40.0	33.0	41.0
23	36.22680997147494	36.0	35.0	40.0	32.0	41.0
24	35.92210232604464	35.0	35.0	39.0	32.0	41.0
25	35.72898520972478	35.0	35.0	39.0	31.0	41.0
26	35.59128789968553	35.0	34.0	39.0	31.0	41.0
27	35.44861951399665	35.0	34.0	39.0	31.0	41.0
28	35.39967194622404	35.0	34.0	38.0	31.0	41.0
29	35.238782159199864	35.0	34.0	38.0	31.0	40.0
30	35.132536454732815	35.0	34.0	37.0	31.0	40.0
31	34.932058088287945	35.0	34.0	37.0	31.0	40.0
32	34.84282538404325	35.0	34.0	37.0	31.0	40.0
33	34.73433066718786	35.0	34.0	37.0	31.0	40.0
34	34.56779912460102	35.0	34.0	36.0	31.0	39.0
35	34.4371221235864	35.0	34.0	36.0	31.0	39.0
36	34.40848407167534	35.0	34.0	36.0	31.0	39.0
37	34.39849379907693	35.0	34.0	35.0	31.0	39.0
38	34.38327460742021	35.0	34.0	35.0	31.0	39.0
39	34.3303521045574	35.0	34.0	35.0	31.0	38.0
40	34.2134299123555	35.0	34.0	35.0	31.0	37.0
41	34.21044449379739	35.0	34.0	35.0	31.0	37.0
42	34.12341297183542	35.0	34.0	35.0	31.0	37.0
43	34.02263291454633	35.0	33.0	35.0	31.0	37.0
44	33.930635822179404	35.0	33.0	35.0	31.0	36.0
45	33.70679061500318	35.0	33.0	35.0	30.0	36.0
46	33.480620286800495	35.0	33.0	35.0	29.0	36.0
47	33.3905461740125	35.0	33.0	35.0	29.0	36.0
48	33.513362982450644	35.0	33.0	35.0	29.0	36.0
49	33.27901445215509	35.0	33.0	35.0	29.0	36.0
50	33.66324926631718	35.0	33.0	35.0	29.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	12.0
22	29.0
23	99.0
24	247.0
25	626.0
26	1525.0
27	3245.0
28	6540.0
29	11978.0
30	21069.0
31	35711.0
32	57534.0
33	92754.0
34	157090.0
35	196583.0
36	241395.0
37	204589.0
38	141531.0
39	83318.0
40	29307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.71408496513345	17.92261976087508	43.36329527399147
2	26.560279990600584	18.06392226494842	30.886502031612544	24.48929571283845
3	23.255933382405345	16.062033044244178	30.21710476148978	30.4649288118607
4	19.37991854875481	18.452348531652227	34.75745923158205	27.410273688010918
5	21.39433513903824	18.527279319880545	32.8455958904003	27.23278965068091
6	25.816885649236767	19.97345131366199	26.557634459136654	27.65202857796459
7	30.725046802563988	17.038234154433678	21.01376765697728	31.222951386025056
8	26.21636090028992	17.5467986734994	24.257033612255345	31.979806813955335
9	29.684497030001882	19.90209977388487	23.170031419576624	27.243371776536623
10	29.189315787753678	15.573543440404736	24.799990040352135	30.43715073148945
11	29.61337891947158	16.85258009346507	23.20177779714376	30.33226318991959
12	31.196184832390017	17.205602924401603	22.84525352750497	28.752958715703407
13	30.09914518209816	16.945951792191945	25.00486310930869	27.950039916401202
14	28.21505992128766	20.879234601061636	20.862038646546104	30.043666831104606
15	25.873453336715464	21.20222286890769	22.82128812483174	30.103035669545108
16	28.827033596693397	20.670159805662372	20.022004596999967	30.480802000644264
17	29.723324094722475	20.79255454074352	18.56945220380552	30.914669160728486
18	29.80362375562759	19.80468196821316	17.840841714740126	32.55085256141913
19	30.833202353589286	18.430017133706716	20.843908975043302	29.8928715376607
20	30.78643869447691	18.057775294782232	21.828591347867157	29.3271946628737
21	30.408283314633056	23.100158265029343	16.94408435821741	29.547474062120195
22	31.313210694794375	21.11079641390429	21.5191419763365	26.056850914964834
23	33.56774817030375	20.51555183452045	20.302508741925294	25.6141912532505
24	33.113261426750675	20.572664190241724	21.676006430197653	24.638067952809944
25	31.88849467656655	21.622589410802323	21.127699718862537	25.361216193768588
26	33.58651019459178	21.758104050251813	20.36853429591507	24.286851459241337
27	29.644587661138594	23.961287111607053	20.203090500728322	26.19103472652603
28	30.840161812246176	20.73119824868038	21.66891582927183	26.759724109801624
29	32.57895698852322	22.346134482614872	19.634708687272926	25.440199841588985
30	32.11917160374981	24.511002642443195	17.375598426163418	25.994227327643575
31	30.452409007167457	21.95415692886577	22.10111906048395	25.492315003482823
32	29.85692835921503	18.48500031571377	23.816380870533575	27.841690454537627
33	28.993379633387057	18.227706159740155	23.486400670483963	29.292513536388824
34	31.69614612159136	19.966936429518846	24.478408803133853	23.858508645755936
35	28.68602079642921	19.222574740592663	22.526345431842497	29.565059031135625
36	27.937678785152237	21.902401344549226	24.042899948221276	26.11701992207726
37	27.869929731702086	22.353829891414414	23.878830553677954	25.897409823205543
38	29.046556893831067	19.821007204649405	22.326493120330298	28.805942781189227
39	28.986541130295418	23.86216772142298	19.260910681888905	27.8903804663927
40	27.849407316289014	25.70713177427697	21.375753085729464	25.067707823704556
41	30.76089906969694	20.771862474486287	20.77697402977393	27.690264426042845
42	28.51086861694646	22.4497607996254	21.293729513961217	27.74564106946692
43	30.089742652269564	20.042736315847915	24.48969643132805	25.377824600554472
44	28.668776956154545	20.59936028204323	22.446032431655816	28.28583033014641
45	29.629433011075523	20.47870719368304	23.540647936783202	26.351211858458235
46	27.756871143745858	22.562507436302756	22.079898469679716	27.600722950271674
47	28.513130091963234	20.02327374207954	22.353443994562358	29.110152171394866
48	27.3208612163794	19.695609355479693	22.204996502656936	30.778532925483976
49	28.947602979563253	22.36225886547399	20.26535939390081	28.424778761061948
50	24.124420216349318	28.370875695933968	19.235266332669987	28.269437755046734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12824.0
1	10429.5
2	8378.5
3	7206.0
4	4480.5
5	2955.5
6	2413.5
7	1987.0
8	1968.5
9	2276.0
10	2306.5
11	2253.0
12	2354.5
13	2199.5
14	2092.0
15	2363.5
16	2406.0
17	2096.0
18	1872.0
19	1731.0
20	1686.5
21	2164.5
22	3018.0
23	3745.0
24	4160.5
25	5050.5
26	6013.5
27	6344.5
28	6482.5
29	6143.0
30	6023.5
31	7779.5
32	10149.0
33	11900.0
34	14665.5
35	16729.5
36	17198.0
37	17123.0
38	16945.5
39	18554.0
40	23000.5
41	29120.0
42	32602.0
43	34197.5
44	35775.0
45	34452.0
46	35290.0
47	39559.5
48	44938.0
49	52827.5
50	63526.0
51	70796.0
52	75212.0
53	79280.5
54	82758.0
55	89630.5
56	93837.5
57	96156.5
58	101194.0
59	102486.5
60	99922.5
61	98515.0
62	98046.0
63	97162.0
64	92347.5
65	90165.0
66	92263.5
67	89241.0
68	80225.0
69	70671.0
70	61977.5
71	56415.5
72	57091.0
73	57129.0
74	50346.5
75	43007.0
76	36997.0
77	27583.5
78	20263.5
79	16768.0
80	13159.0
81	9620.5
82	7774.5
83	7290.5
84	6806.5
85	5423.0
86	3969.5
87	2887.0
88	1545.5
89	874.0
90	570.0
91	285.5
92	176.5
93	114.0
94	61.0
95	24.0
96	22.5
97	24.0
98	14.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	8942.0
25	7434.0
26	7008.0
27	6520.0
28	7894.0
29	9140.0
30	9360.0
31	9429.0
32	11973.0
33	11003.0
34	10414.0
35	7979.0
36	7861.0
37	8618.0
38	7948.0
39	9039.0
40	9940.0
41	11358.0
42	15603.0
43	21932.0
44	68593.0
45	134703.0
46	131434.0
47	176339.0
48	182047.0
49	263532.0
50	129143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.546666189326087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.78500700302881	6.28027502120395
2	12.015298150210159	2.294120812105672
3	5.217974449548298	1.494427807628104
4	2.8972332306525255	1.106356741026498
5	1.8147071180698884	0.8662201543803592
6	1.2206436966287562	0.6991846744691853
7	0.8609352071970191	0.5753342723623762
8	0.6477007594021008	0.4946706352467894
9	0.5104740544660875	0.43859928566696416
>10	4.368814773952886	9.105343153769976
>50	1.7470354862604138	12.397506386069065
>100	2.7459248736828012	50.68569819259285
>500	0.13476430819680613	8.485848988680878
>1k	0.0334868887034488	5.076413874797334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	3388	0.26361942940554906	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	3130	0.24354451417927056	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	2891	0.22494798418283424	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	2627	0.20440621046292134	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	2384	0.18549844147072875	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	2308	0.1795849005513599	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2244	0.17460507661925978	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	2032	0.1581094098441782	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	1832	0.14254746005636537	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	1747	0.13593363139654493	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	1693	0.13173190495383547	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	1610	0.12527369579189315	No Hit
CTCTCGCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAACCCTGCGT	1609	0.1251958860429541	No Hit
AGAGTGCAGTGCTACGAGCGGGATGGCACATGCAGCGCAAGTAGGTCTAC	1599	0.12441778855356346	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	1554	0.12091634985130557	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1514	0.11780395989374301	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	1493	0.11616995516602266	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	1489	0.1158587161702664	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC	1440	0.11204603847225227	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	1386	0.1078443120295428	No Hit
CTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACGTCCCTAAAAC	1344	0.10457630257410212	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	1340	0.10426506357834586	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG	1335	0.10387601483365053	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	1326	0.10317572709319896	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	1320	0.10270886859956457	No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCG	1311	0.102008580859113	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	1309	0.10185296136123487	No Hit
ACCCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGTTCTTCCTGAGGTCT	1287	0.10014114688457545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.012760798826006508	0.0	0.0	0.0	0.0
2	0.02637750489034272	0.0	0.0	0.0	0.0
3	0.03532562601833509	0.0	0.0	0.0	0.0
4	0.04419593739738839	0.0	0.0	0.0	0.0
5	0.05314405852538076	0.0	0.0	0.0	0.0
6	0.06707200358547323	0.0	0.0	0.0	0.0
7	0.07897689517315003	0.0	0.0	0.0	0.0
8	0.0887809235394721	0.0	0.0	0.0	0.0
9	0.09944085914412389	0.0	0.0	0.0	0.0
10	0.10597687805500526	0.0	0.0	0.0	0.0
11	0.11445814068936325	0.0	0.0	0.0	0.0
12	0.1219278765875134	0.0	0.0	0.0	0.0
13	0.12955323198354168	0.0	0.0	0.0	0.0
14	0.13499991440927617	0.0	0.0	0.0	0.0
15	0.1440258452862076	0.0	0.0	0.0	0.0
16	0.15437454189510313	0.0	0.0	0.0	0.0
17	0.1624667557847658	0.0	0.0	0.0	0.0
18	0.17763965682788327	0.0	0.0	0.0	0.0
19	0.1862765389601194	0.0	0.0	0.0	0.0
20	0.196703045317954	0.0	0.0	0.0	0.0
21	0.20930822464608237	0.0	0.0	0.0	0.0
22	0.21732262878680594	0.0	0.0	0.0	0.0
23	0.228293803387214	0.0	0.0	0.0	0.0
24	0.2388759292429267	0.0	0.0	0.0	0.0
25	0.24945805509863941	0.0	0.0	0.0	0.0
26	0.26377504890342723	0.0	0.0	0.0	0.0
27	0.27427936501020084	0.0	0.0	0.0	0.0
28	0.28657330534257297	0.0	0.0	0.0	0.0
29	0.30221306487932487	0.0	0.0	0.0	0.0
30	0.31831968290971113	0.0	0.0	0.0	0.0
31	0.329057428263302	0.0	0.0	0.0	0.0
32	0.34267413432763816	0.0	0.0	0.0	0.0
33	0.3552015039068275	0.0	0.0	0.0	0.0
34	0.36656172725193087	0.0	0.0	0.0	0.0
35	0.37496518013734975	0.0	0.0	0.0	0.0
36	0.3858585449888187	0.0	0.0	0.0	0.0
37	0.39270580289545637	0.0	0.0	0.0	0.0
38	0.39659629034240956	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTTT	80	0.0	398.72006	44
TATAAAT	10	3.4293378E-4	398.72006	44
ACCTTAA	10	3.4293378E-4	398.72006	44
TTTGTAC	70	0.0	398.72	44
GAATATG	15	2.169636E-6	398.72	44
CCCGTAC	40	0.0	348.88004	44
TATGTTC	40	0.0	348.88004	44
GGTTATC	35	1.8189894E-12	341.76	44
ACGCTTA	30	2.6011548E-10	332.26666	44
GCGAGAC	50	0.0	318.976	44
GGTCGAG	20	6.8433983E-6	299.04004	44
GTTAGTC	20	6.8433983E-6	299.04004	44
CTATCAT	580	0.0	295.60278	44
TTTACTA	15	0.00115523	265.81335	44
CAAAAAT	45	9.094947E-12	265.81335	44
TTTACCG	30	1.0363692E-7	265.81335	44
GAATCAA	15	0.00115523	265.81335	44
TGCATAA	30	1.0363692E-7	265.81335	44
CCTATGA	15	0.00115523	265.81335	44
GGTAGCC	30	1.0363692E-7	265.81335	44
>>END_MODULE