FastQCFastQC Report
Tue 24 May 2016
ERR840951.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840951.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4837074
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA64690.133737875418073No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG57830.11955574795837318No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC54180.11200986381436381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT29950.0159.5699644
TAATCCG150.007068595145.1517344
ACGACAC2350.0138.9750544
CCGAATA251.8634359E-4130.6365544
AATGCGC359.1804395E-999.11400643
CCTATCA28200.089.1850443
CGTAATC1100.089.0703744
GACATCC1600.083.1112343
AACGACA6500.082.72208443
TCCACGA2950.081.18655444
ACTACGG1900.080.2154244
TGTTTCG701.520877E-677.7598544
TGATTAG2650.069.8371544
CCCTATC37600.066.6322342
AACAACG500.002927296665.31827544
GGATAGG1001.6535705E-765.31827544
CGAATGT552.1075357E-763.0725543
TAGTAGG701.421741E-462.2078844
CTAATCG750.061.95665742
ATTCGTC1100.060.54225541
Produced by FastQC (version 0.11.5)