##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840951.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4837074
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.34194391071958	39.0	37.0	39.0	35.0	39.0
2	37.81002916225801	39.0	38.0	39.0	35.0	39.0
3	37.94895054324164	39.0	38.0	39.0	35.0	39.0
4	38.455026530501705	39.0	38.0	40.0	35.0	41.0
5	39.16007425149998	40.0	39.0	41.0	35.0	41.0
6	39.19249116304609	40.0	38.0	41.0	35.0	41.0
7	39.192905876569185	40.0	38.0	41.0	35.0	41.0
8	39.13249993694535	40.0	38.0	41.0	35.0	41.0
9	39.04984190855877	40.0	38.0	41.0	35.0	41.0
10	38.937572176898676	40.0	38.0	41.0	35.0	41.0
11	38.846847701730425	40.0	38.0	41.0	35.0	41.0
12	38.809978305066245	40.0	38.0	41.0	35.0	41.0
13	38.68700127391063	40.0	38.0	41.0	35.0	41.0
14	38.65760829790902	40.0	38.0	41.0	35.0	41.0
15	38.67079023393068	40.0	38.0	41.0	35.0	41.0
16	38.63516683846474	40.0	38.0	41.0	35.0	41.0
17	38.65232473185235	40.0	38.0	41.0	35.0	41.0
18	38.66021317846285	40.0	38.0	41.0	35.0	41.0
19	38.78166221976343	40.0	38.0	41.0	35.0	41.0
20	38.788688574952545	40.0	38.0	41.0	35.0	41.0
21	38.74616782790588	40.0	38.0	41.0	35.0	41.0
22	38.67830655474777	40.0	38.0	41.0	35.0	41.0
23	38.49985714504265	40.0	37.0	41.0	35.0	41.0
24	38.311840794662224	40.0	37.0	41.0	34.0	41.0
25	38.28403039601114	40.0	37.0	41.0	34.0	41.0
26	38.23209911878282	40.0	37.0	41.0	34.0	41.0
27	38.157452397606605	40.0	36.0	41.0	34.0	41.0
28	38.0897568585606	40.0	36.0	41.0	34.0	41.0
29	37.948271782036485	40.0	35.0	41.0	33.0	41.0
30	37.87577916428936	40.0	35.0	41.0	33.0	41.0
31	37.72728234855293	39.0	35.0	41.0	33.0	41.0
32	37.61474975462319	39.0	35.0	41.0	33.0	41.0
33	37.515676829658204	39.0	35.0	41.0	33.0	41.0
34	37.455515233404924	39.0	35.0	41.0	33.0	41.0
35	37.27584448949991	39.0	35.0	41.0	33.0	41.0
36	37.24818459533307	39.0	35.0	41.0	33.0	41.0
37	37.147494437098764	38.0	35.0	41.0	33.0	41.0
38	37.090268072528964	38.0	35.0	41.0	33.0	41.0
39	36.99311922450129	38.0	35.0	41.0	33.0	41.0
40	36.92502188263613	38.0	35.0	40.0	33.0	41.0
41	36.83091305758567	37.0	35.0	40.0	33.0	41.0
42	36.726370594960564	37.0	35.0	40.0	33.0	41.0
43	36.62529491330901	37.0	35.0	40.0	32.0	41.0
44	36.45178863736271	37.0	35.0	40.0	32.0	41.0
45	36.30795481280907	36.0	35.0	40.0	32.0	41.0
46	36.01796846452813	36.0	34.0	40.0	31.0	41.0
47	35.85863214722111	36.0	34.0	40.0	31.0	41.0
48	35.85645693312169	36.0	34.0	40.0	31.0	41.0
49	35.819401246380195	36.0	34.0	40.0	31.0	41.0
50	35.896749890748886	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	15.0
21	59.0
22	124.0
23	258.0
24	493.0
25	989.0
26	1992.0
27	4038.0
28	8542.0
29	16690.0
30	31178.0
31	56196.0
32	95505.0
33	167554.0
34	312094.0
35	273714.0
36	444120.0
37	662054.0
38	746816.0
39	990124.0
40	1024517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.53327610865577	17.020723685434625	36.446000205909606
2	27.073639973256558	19.649399616379654	32.052538373405085	21.224422036958707
3	23.055983844778886	18.72592811273923	29.757700626453097	28.46038741602878
4	21.091283697541115	19.43921056407241	33.97173580557172	25.497769932814755
5	21.460143053424446	20.981733998694253	29.876491449169478	27.681631498711823
6	23.594181110315866	22.3459264836552	28.060000735982126	25.99989167004681
7	27.60247207299289	21.406122792415413	23.05335829056988	27.93804684402182
8	26.53308591102803	21.567625386752404	23.748096473198466	28.1511922290211
9	26.810815794837957	20.320197706299304	24.890667374532622	27.97831912433012
10	26.226557625539737	18.876494343481205	25.652264158042655	29.244683872936406
11	28.12164957575592	20.90557225297773	24.52048490471719	26.45229326654916
12	28.604958286765925	20.905675621253675	23.855806216733505	26.633559875246892
13	28.920148833778438	19.481240105071784	24.91619933869112	26.68241172245866
14	26.223746008434023	21.849014507530793	23.523249799362176	28.403989684673007
15	26.428063742667575	23.65779394733262	22.528660921871364	27.38548138812844
16	27.260591837131294	24.210607487088268	21.43769146388912	27.09110921189132
17	27.52494586603389	23.983548732146748	21.01369546961655	27.477809932202813
18	26.38648902208236	22.923155610189134	21.232650151723956	29.457705216004555
19	27.702284480245705	20.918927434229868	23.188770732058263	28.190017353466168
20	27.42079199119137	21.517347057332596	24.779153678442796	26.28270727303324
21	28.423939761930455	25.181855807870622	20.579155911197557	25.815048519001365
22	30.939944272095072	23.89072402034784	20.49683341623469	24.6724982913224
23	30.095611520518396	23.306982692429347	21.71941136315053	24.877994423901722
24	29.528181706544082	23.27237499364285	22.12750931658271	25.07193398323036
25	28.7752985263051	25.207948989453566	21.262553903546138	24.754198580695196
26	29.43519374580382	23.757254470473608	22.43677817508812	24.37077360863446
27	30.001867934705075	24.509509552626486	21.382494405125833	24.10612810754261
28	29.930447805041123	23.562103972891613	20.859052759716963	25.648395462350305
29	28.90817254949901	25.762172571995904	21.901174529013677	23.428480349491412
30	29.08101597805381	25.54429228743902	21.203319644050122	24.171372090457048
31	27.80039778127021	24.569425221982684	21.74111692816146	25.889060068585646
32	26.15766547618687	24.155858325255128	24.42316426326561	25.263311935292393
33	27.5682084985717	22.622024318111432	24.136270118596244	25.673497064720625
34	30.510780348921923	22.28080806439108	23.036323100126445	24.172088486560554
35	25.885506092108514	22.959272481327993	23.322549659492616	27.832671767070877
36	27.459968127968544	23.12412632712092	24.250412301314135	25.165493243596405
37	25.77509608241229	26.282998575861523	23.060874097397484	24.881031244328703
38	28.046251136329392	23.343792886454324	22.337030911308055	26.272925065908233
39	26.556717990341593	24.663830986068596	22.35317856816096	26.426272455428844
40	26.537186804770414	23.8537373718954	22.79352365148255	26.815552171851635
41	25.947444084024017	24.133742429409327	22.773196179523076	27.14561730704358
42	25.421652526512357	24.638753027511555	23.105130201226075	26.834464244750016
43	26.687941725856223	24.48975576622418	22.988072303971798	25.834230203947794
44	26.164837253074406	24.553703889799877	22.69600808888631	26.585450768239404
45	28.859215289335893	22.1672117674914	24.42729484370131	24.5462780994714
46	26.929997453244898	23.76523900742988	23.484320846851436	25.820442692473783
47	25.939861560412574	23.931548114908722	23.77953755927479	26.34905276540392
48	24.5940802283786	23.76168980972448	22.550627521380505	29.09360244051641
49	25.772339463415655	25.681660961826985	21.635447268414953	26.910552306342407
50	23.333474542260117	27.396835730912265	25.31058531843356	23.95910440839405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57072.0
1	50441.5
2	49256.0
3	48390.5
4	37667.0
5	27992.0
6	19026.5
7	16196.0
8	17790.5
9	19448.0
10	20324.5
11	18336.5
12	15355.5
13	14460.0
14	15384.0
15	16144.5
16	15857.5
17	15038.5
18	15321.5
19	16969.0
20	18005.0
21	18791.0
22	19773.5
23	22863.0
24	27578.0
25	31768.0
26	35902.5
27	40112.0
28	44273.5
29	50356.5
30	58893.5
31	69377.0
32	79242.0
33	83841.0
34	87950.5
35	92325.0
36	95407.5
37	104831.5
38	115154.5
39	117271.5
40	122596.5
41	147298.5
42	175342.0
43	185502.5
44	183917.5
45	179011.0
46	184532.0
47	200060.0
48	221203.0
49	248213.0
50	272093.5
51	290912.5
52	296507.5
53	289352.5
54	291392.5
55	300889.0
56	311009.0
57	314067.0
58	305756.5
59	296542.5
60	294028.0
61	298381.0
62	293649.0
63	283378.0
64	275188.0
65	264001.5
66	246251.0
67	234289.5
68	229172.5
69	216131.5
70	197378.0
71	181851.5
72	166032.5
73	152284.0
74	142626.0
75	128919.0
76	111604.0
77	87200.0
78	65524.0
79	56138.0
80	49193.5
81	38340.5
82	28109.0
83	20781.0
84	15476.5
85	12131.0
86	9538.5
87	7482.5
88	4928.0
89	2677.5
90	1520.0
91	810.5
92	567.0
93	444.0
94	306.0
95	322.0
96	445.5
97	444.0
98	222.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	32891.0
25	24544.0
26	20372.0
27	20955.0
28	26551.0
29	35597.0
30	27378.0
31	29360.0
32	37409.0
33	28964.0
34	31712.0
35	25236.0
36	21821.0
37	27950.0
38	30698.0
39	28596.0
40	35332.0
41	31741.0
42	50492.0
43	70133.0
44	191215.0
45	395687.0
46	557389.0
47	553941.0
48	831475.0
49	772619.0
50	897016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.664469991528716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95982588298456	21.854153177677603
2	8.825433119541195	4.706509931564998
3	2.6908536859618004	2.152505620827686
4	1.1556201357168843	1.232559937217168
5	0.6423505442844787	0.856396840605781
6	0.4468816604845669	0.7149517575453155
7	0.3139869905161919	0.5860607680444587
8	0.2394059631760287	0.5106906496720197
9	0.1908083830094493	0.45790239625988205
>10	1.973758141955949	12.708190733657847
>50	0.9280905829103424	17.996215968143908
>100	0.5993130118177974	26.216338639570854
>500	0.022447931760516078	3.9490141329740913
>1k	0.010522468012741912	4.956780891709411
>5k	7.014978675161274E-4	1.1017285545290545
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	6469	0.133737875418073	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	5783	0.11955574795837318	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	5418	0.11200986381436381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.021831379879654517	0.0	0.0	0.0	0.0
2	0.04236031948239783	0.0	0.0	0.0	0.0
3	0.05453710238875816	0.0	0.0	0.0	0.0
4	0.06599030736350116	0.0	0.0	0.0	0.0
5	0.07585164088868601	0.0	0.0	0.0	0.0
6	0.09197709193615809	0.0	0.0	0.0	0.0
7	0.10644865056850485	0.0	0.0	0.0	0.0
8	0.11978315816545292	0.0	0.0	0.0	0.0
9	0.13090558465717084	0.0	0.0	0.0	0.0
10	0.14167655901067464	0.0	0.0	0.0	0.0
11	0.15153789253585948	0.0	0.0	0.0	0.0
12	0.16288772923465716	0.0	0.0	0.0	0.0
13	0.17322455682919055	0.0	0.0	0.0	0.0
14	0.18436765697609753	0.0	0.0	0.0	0.0
15	0.196585787192836	0.0	0.0	0.0	0.0
16	0.2079149502364446	0.0	0.0	0.0	0.0
17	0.22211775135133346	0.0	0.0	0.0	0.0
18	0.24293612212672372	0.0	0.0	0.0	0.0
19	0.2546580846189246	0.0	0.0	0.0	0.0
20	0.26774450835360386	0.0	0.0	0.0	0.0
21	0.28157518367508955	0.0	0.0	0.0	0.0
22	0.2945995864442016	0.0	0.0	0.0	0.0
23	0.3076033155581246	0.0	0.0	0.0	0.0
24	0.3187464157050316	0.0	0.0	0.0	0.0
25	0.3327011329576517	0.0	0.0	0.0	0.0
26	0.3494674673159848	0.0	0.0	0.0	0.0
27	0.3616235765671561	0.0	0.0	0.0	0.0
28	0.37522684168156206	0.0	0.0	0.0	0.0
29	0.3901325470728792	0.0	0.0	0.0	0.0
30	0.40658877660337633	0.0	0.0	0.0	0.0
31	0.4211016825461012	0.0	0.0	0.0	0.0
32	0.43553189386806984	0.0	0.0	0.0	0.0
33	0.45062366215608857	0.0	0.0	0.0	0.0
34	0.46267640313131453	0.0	0.0	0.0	0.0
35	0.47423297638200285	0.0	0.0	0.0	0.0
36	0.48492125611475034	0.0	0.0	0.0	0.0
37	0.49294263432810825	0.0	0.0	0.0	0.0
38	0.4986485631602907	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	2995	0.0	159.56996	44
TAATCCG	15	0.007068595	145.15173	44
ACGACAC	235	0.0	138.97505	44
CCGAATA	25	1.8634359E-4	130.63655	44
AATGCGC	35	9.1804395E-9	99.114006	43
CCTATCA	2820	0.0	89.18504	43
CGTAATC	110	0.0	89.07037	44
GACATCC	160	0.0	83.11123	43
AACGACA	650	0.0	82.722084	43
TCCACGA	295	0.0	81.186554	44
ACTACGG	190	0.0	80.21542	44
TGTTTCG	70	1.520877E-6	77.75985	44
TGATTAG	265	0.0	69.83715	44
CCCTATC	3760	0.0	66.63223	42
AACAACG	50	0.0029272966	65.318275	44
GGATAGG	100	1.6535705E-7	65.318275	44
CGAATGT	55	2.1075357E-7	63.07255	43
TAGTAGG	70	1.421741E-4	62.20788	44
CTAATCG	75	0.0	61.956657	42
ATTCGTC	110	0.0	60.542255	41
>>END_MODULE