FastQCFastQC Report
Tue 24 May 2016
ERR840950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244841
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC58350.25992932238853445No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA46370.20656251378159968No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC38860.1731080285864344No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38350.1708361527609305No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC28850.12851689718781864No Hit
AACTTTCCAGAACGCTCGGTGAGAGGCGGAGGAGCGG25350.11292559250298795No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA22610.10071982826400623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCG153.2849373E-5201.9198644
CACGCTA1150.0175.5824944
CTATCAT9000.0150.318144
TAAGCCA18350.0143.5996944
CCAACGA501.4551915E-11141.3438944
CGCACTA150.008853467134.6132444
AACGTAT502.2700988E-9121.1519144
GTTAGTA153.085928E-4115.0860643
GACGATA303.246896E-9115.0860643
TGATAGG206.7232304E-6115.0860543
GCTAGTA401.8189894E-12115.0860543
GCTAACC451.7145976E-7112.17770444
TAGGATA554.4037733E-9110.138144
GCTTATC1200.0109.3732544
AAGTCGA850.0106.8987444
TTAAGCC15050.0100.5569343
GACGATC1700.095.021144
AGACGAT1000.092.0688543
AACACGC455.293259E-1089.5113843
GTATTTA1150.087.79124544
Produced by FastQC (version 0.11.5)