##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840950.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244841 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.10034162775894 39.0 37.0 39.0 32.0 39.0 2 37.632382872550885 39.0 38.0 39.0 35.0 39.0 3 37.83800322606367 39.0 38.0 39.0 35.0 39.0 4 38.187134857212605 39.0 38.0 40.0 35.0 41.0 5 38.81067746000719 40.0 38.0 41.0 35.0 41.0 6 38.80435095403193 40.0 38.0 41.0 35.0 41.0 7 38.72059179246993 40.0 38.0 41.0 35.0 41.0 8 38.620176662846056 40.0 38.0 41.0 35.0 41.0 9 38.44370848536712 40.0 37.0 41.0 35.0 41.0 10 38.23005148248807 40.0 37.0 41.0 34.0 41.0 11 38.09352555481658 40.0 36.0 41.0 34.0 41.0 12 37.96917599063809 40.0 36.0 41.0 34.0 41.0 13 37.80290497188888 39.0 35.0 41.0 33.0 41.0 14 37.709206576323226 39.0 35.0 41.0 33.0 41.0 15 37.66397798329592 39.0 35.0 41.0 33.0 41.0 16 37.53781581858136 39.0 35.0 41.0 33.0 41.0 17 37.4705179565056 39.0 35.0 41.0 33.0 41.0 18 37.40717583116132 39.0 35.0 41.0 33.0 41.0 19 37.33351226211567 38.0 35.0 41.0 33.0 41.0 20 37.23045195628555 38.0 35.0 41.0 33.0 41.0 21 37.10550724973395 38.0 35.0 40.0 33.0 41.0 22 36.96652190511488 37.0 35.0 40.0 33.0 41.0 23 36.782885291207705 37.0 35.0 40.0 33.0 41.0 24 36.56804067637753 37.0 35.0 40.0 33.0 41.0 25 36.44037148040668 36.0 35.0 40.0 33.0 41.0 26 36.32407613640565 36.0 35.0 40.0 33.0 41.0 27 36.244021225492126 35.0 35.0 40.0 33.0 41.0 28 36.14275984185959 35.0 35.0 39.0 33.0 41.0 29 35.95923815630062 35.0 35.0 39.0 33.0 41.0 30 35.85532662986481 35.0 35.0 39.0 33.0 41.0 31 35.73577290051207 35.0 35.0 39.0 32.0 41.0 32 35.654508805490956 35.0 35.0 39.0 32.0 41.0 33 35.497147913414295 35.0 35.0 38.0 32.0 41.0 34 35.43466828412185 35.0 35.0 38.0 32.0 41.0 35 35.29265204047158 35.0 34.0 37.0 32.0 40.0 36 35.238130454286825 35.0 34.0 37.0 32.0 40.0 37 35.20256126453377 35.0 34.0 37.0 32.0 40.0 38 35.085050798258344 35.0 34.0 36.0 32.0 40.0 39 35.03523289582477 35.0 34.0 36.0 32.0 39.0 40 34.9405128139999 35.0 34.0 36.0 32.0 39.0 41 34.87936515283266 35.0 34.0 36.0 32.0 39.0 42 34.834277674504676 35.0 34.0 36.0 32.0 39.0 43 34.716469748003334 35.0 34.0 36.0 32.0 39.0 44 34.55832086697464 35.0 34.0 35.0 31.0 39.0 45 34.364635058402854 35.0 34.0 35.0 31.0 38.0 46 34.117066694035096 35.0 34.0 35.0 30.0 37.0 47 34.02642410279057 35.0 34.0 35.0 30.0 37.0 48 33.965337172856124 35.0 34.0 35.0 30.0 37.0 49 34.08608701183056 35.0 34.0 35.0 31.0 37.0 50 33.94997862523728 35.0 33.0 35.0 30.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 7.0 21 18.0 22 69.0 23 158.0 24 447.0 25 1012.0 26 2122.0 27 4503.0 28 8939.0 29 16481.0 30 28151.0 31 47235.0 32 76105.0 33 126508.0 34 247073.0 35 312361.0 36 361341.0 37 362556.0 38 302900.0 39 241112.0 40 105742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 46.83859569564169 17.37615269856529 35.785251605793015 2 27.07109323110189 21.960308101999207 29.538662203692823 21.429936463206083 3 23.99684431993179 19.96943213350077 26.963557775361373 29.07016577120607 4 21.39594741899315 19.682062114866934 31.036362931717658 27.88562753442226 5 22.560573332365188 21.379554275781672 31.115878585610297 24.943993806242847 6 25.244861440075265 24.51028825649567 25.786369725071843 24.458480578357218 7 29.010161521461875 22.417311515604 21.48642153274998 27.086105430184144 8 25.84374572631202 21.91651880912724 23.91835323748987 28.321382227070867 9 25.232655675836284 22.219613772200347 26.474614460445085 26.073116091518283 10 27.956679337200274 20.58012126471318 23.377245871756617 28.085953526329927 11 27.827716974164318 23.19130842674381 23.969359077101675 25.011615521990198 12 27.451120146148433 21.845333366594783 24.11631826040241 26.587228226854375 13 26.79049429335975 20.992266267410475 24.222829144692206 27.994410294537563 14 24.064020569831005 22.062854340240577 23.77246317222467 30.100661917703746 15 25.115008145343033 23.089430387274646 23.882404143545134 27.91315732383719 16 27.685880648117173 25.712244207941676 21.067104529897662 25.534770614043488 17 26.81183210748556 23.46829909111603 21.31451626195352 28.405352539444888 18 26.293443500007353 23.199460451764736 20.626494259504348 29.880601788723567 19 27.25787706122616 23.319825323931628 21.64995204560145 27.772345569240763 20 27.529744868344796 20.52915997168619 24.5116692006249 27.429425959344112 21 29.512958824255257 23.681098126771563 21.04104477778159 25.764898271191587 22 28.406510750650042 25.982419244837384 20.401578552779462 25.209491451733108 23 30.494409180872946 23.27919883858144 21.65195664191807 24.574435338627545 24 28.133172906232556 25.287715254666143 20.888517271379133 25.69059456772217 25 28.16904189340849 25.97580692567924 20.949441527420596 24.90570965349167 26 30.047287676618662 22.896018511087448 22.985066147740625 24.07162766455327 27 27.01468221706771 26.210424451028587 21.122658244513 25.6522350873907 28 27.249322868791715 23.47024689332974 22.7948195884369 26.485610649441643 29 30.46164637479433 24.30608737903537 22.59729572612571 22.63497052004459 30 28.71072997492286 25.779612409118908 20.301826051285254 25.20783156467298 31 26.783923103827295 23.07679868488472 24.444030673086388 25.695247538201592 32 25.769748449069812 23.661003935653827 24.849415229139826 25.71983238613653 33 25.251241856286335 24.07745977529185 25.08847303347564 25.58282533494618 34 28.710175393582592 21.73824864883221 24.475014892441905 25.076561065143288 35 26.404889738078936 24.374800723596085 22.53720499370631 26.68310454461867 36 25.354804836438877 25.170970856485763 25.360919896063095 24.11330441101227 37 26.43518554072527 26.93949959297771 21.226356228976915 25.398958637320103 38 26.35370657032667 25.77996517656832 21.738370253737724 26.127957999367286 39 26.078881734721936 25.16262046545159 22.826423846014084 25.932073953812385 40 25.502561041493465 26.27761435785414 23.919238535883398 24.300586064768996 41 26.91359653830738 23.851477709051196 21.85774483803844 27.377180914602985 42 25.276939769041977 25.71500294439971 22.28972595501989 26.718331331538415 43 25.135725065710655 24.91694885735982 24.45736323224658 25.489962844682946 44 26.599866379008947 24.807671008583284 22.26438071672503 26.328081895682736 45 27.711316468887652 25.528364730818325 24.061953994579667 22.698364805714352 46 25.858920456958423 26.965100531796928 22.777376688702972 24.398602322541677 47 28.029873037491065 22.759959603792428 23.334478779043522 25.87568857967298 48 25.25996066007402 25.237861473526525 22.25649564258024 27.245682223819212 49 25.58593054318789 24.484035110036892 22.20442691769495 27.725607429080267 50 21.592054347272335 30.8363623476356 24.361470495074943 23.210112810017122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23756.0 1 19471.5 2 15971.5 3 14481.5 4 10214.0 5 7807.5 6 6606.5 7 5232.5 8 4889.0 9 5289.0 10 5360.0 11 5348.0 12 5516.5 13 5415.5 14 5401.5 15 5940.0 16 6989.5 17 8110.0 18 8243.5 19 7962.5 20 8542.0 21 10216.0 22 11648.5 23 13008.0 24 14851.5 25 16346.5 26 18887.0 27 23252.0 28 26164.0 29 26469.0 30 26324.5 31 28095.5 32 32326.5 33 37372.0 34 46331.0 35 54563.0 36 55977.5 37 56752.5 38 58827.5 39 63413.5 40 72133.5 41 82150.0 42 90102.5 43 102777.5 44 113017.0 45 102475.0 46 91017.5 47 94183.5 48 103043.5 49 110803.5 50 121989.0 51 130849.0 52 129291.0 53 124863.0 54 129022.5 55 137478.0 56 138564.5 57 145110.5 58 151794.5 59 151393.0 60 151794.0 61 145745.0 62 133077.0 63 125891.5 64 123991.0 65 120945.5 66 110402.0 67 100047.5 68 94304.0 69 85447.0 70 76609.5 71 70671.0 72 69319.0 73 68061.0 74 62501.5 75 56198.0 76 49714.5 77 38101.0 78 27592.5 79 21647.0 80 16486.0 81 12909.0 82 10391.0 83 9389.5 84 8242.5 85 6405.5 86 4974.5 87 3881.0 88 2250.0 89 777.0 90 311.5 91 285.5 92 284.0 93 278.0 94 187.5 95 66.5 96 36.0 97 36.0 98 18.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 16352.0 25 10575.0 26 7553.0 27 9532.0 28 11036.0 29 12910.0 30 12510.0 31 12761.0 32 17911.0 33 13498.0 34 15714.0 35 11296.0 36 9769.0 37 12979.0 38 11971.0 39 11260.0 40 16300.0 41 15223.0 42 23244.0 43 33174.0 44 90015.0 45 173874.0 46 266537.0 47 243283.0 48 399464.0 49 334633.0 50 451467.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.402643759472184 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32283674561026 10.27237409562017 2 11.095118619892409 3.195980819027941 3 4.317823439759356 1.8656421845745845 4 2.1765611810479997 1.2539294124532152 5 1.3818340213058036 0.9951031571793193 6 0.9457046586286091 0.8172388379940665 7 0.7000219985498072 0.705751722823464 8 0.5202880202580749 0.5994818406478479 9 0.4314060579871952 0.5592048991980996 >10 3.7521963111283565 12.1226156561498 >50 1.649062387953798 17.478213134460052 >100 1.6400857435106875 40.44178695723144 >500 0.04857948757340092 4.76644662580467 >1k 0.01795328888582208 4.4824706277711215 >5k 5.280379084065317E-4 0.4437600290642092 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC 5835 0.25992932238853445 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 4637 0.20656251378159968 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 3886 0.1731080285864344 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3835 0.1708361527609305 No Hit CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC 2885 0.12851689718781864 No Hit AACTTTCCAGAACGCTCGGTGAGAGGCGGAGGAGCGG 2535 0.11292559250298795 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 2261 0.10071982826400623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.013319428859326785 0.0 0.0 0.0 0.0 2 0.026683404303467374 0.0 0.0 0.0 0.0 3 0.03621637345362099 0.0 0.0 0.0 0.0 4 0.04610571528228503 0.0 0.0 0.0 0.0 5 0.056128696865390466 0.0 0.0 0.0 0.0 6 0.0727891195857524 0.0 0.0 0.0 0.0 7 0.08535125650324454 0.0 0.0 0.0 0.0 8 0.09595334368892941 0.0 0.0 0.0 0.0 9 0.10504084699094501 0.0 0.0 0.0 0.0 10 0.11568748076144368 0.0 0.0 0.0 0.0 11 0.12588864868380434 0.0 0.0 0.0 0.0 12 0.13568889734284076 0.0 0.0 0.0 0.0 13 0.14646917086778083 0.0 0.0 0.0 0.0 14 0.15756127048641752 0.0 0.0 0.0 0.0 15 0.16682696012768833 0.0 0.0 0.0 0.0 16 0.17774087340706982 0.0 0.0 0.0 0.0 17 0.18936753204347212 0.0 0.0 0.0 0.0 18 0.20843347034377935 0.0 0.0 0.0 0.0 19 0.22210927188161655 0.0 0.0 0.0 0.0 20 0.2320431602950944 0.0 0.0 0.0 0.0 21 0.24407073819482092 0.0 0.0 0.0 0.0 22 0.2566328751123131 0.0 0.0 0.0 0.0 23 0.26803680082464637 0.0 0.0 0.0 0.0 24 0.27854979484070364 0.0 0.0 0.0 0.0 25 0.29235923613298226 0.0 0.0 0.0 0.0 26 0.3079950874026267 0.0 0.0 0.0 0.0 27 0.3213145162619535 0.0 0.0 0.0 0.0 28 0.3344112121972113 0.0 0.0 0.0 0.0 29 0.346394243512124 0.0 0.0 0.0 0.0 30 0.3613173494247477 0.0 0.0 0.0 0.0 31 0.3737013000029846 0.0 0.0 0.0 0.0 32 0.387109822031939 0.0 0.0 0.0 0.0 33 0.40136472917235566 0.0 0.0 0.0 0.0 34 0.41169953684915767 0.0 0.0 0.0 0.0 35 0.42234617061965635 0.0 0.0 0.0 0.0 36 0.4326364317116446 0.0 0.0 0.0 0.0 37 0.44003116479073573 0.0 0.0 0.0 0.0 38 0.4453322083835782 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACTCG 15 3.2849373E-5 201.91986 44 CACGCTA 115 0.0 175.58249 44 CTATCAT 900 0.0 150.3181 44 TAAGCCA 1835 0.0 143.59969 44 CCAACGA 50 1.4551915E-11 141.34389 44 CGCACTA 15 0.008853467 134.61324 44 AACGTAT 50 2.2700988E-9 121.15191 44 GTTAGTA 15 3.085928E-4 115.08606 43 GACGATA 30 3.246896E-9 115.08606 43 TGATAGG 20 6.7232304E-6 115.08605 43 GCTAGTA 40 1.8189894E-12 115.08605 43 GCTAACC 45 1.7145976E-7 112.177704 44 TAGGATA 55 4.4037733E-9 110.1381 44 GCTTATC 120 0.0 109.37325 44 AAGTCGA 85 0.0 106.89874 44 TTAAGCC 1505 0.0 100.55693 43 GACGATC 170 0.0 95.0211 44 AGACGAT 100 0.0 92.06885 43 AACACGC 45 5.293259E-10 89.51138 43 GTATTTA 115 0.0 87.791245 44 >>END_MODULE