FastQCFastQC Report
Tue 24 May 2016
ERR840949.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840949.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467142
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC208960.46777111629762386No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170380.38140717263968776No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG133680.2992517363450725No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA120820.2704637551257605No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104480.23388555814881193No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC87510.19589706349160155No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71860.1608634782597016No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA65310.1462008595204719No Hit
TCAGAACTGCGTCTCGCGACCCAGGCGCGGG61150.13688841769525123No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA61100.13677648930792888No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA60940.1364183184684973No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG58960.1319859543305317No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA52500.11752480668848225No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48100.10767510860411422No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC45600.10207868923799601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT48350.0220.726344
TCGTTAC150.0029866376193.5984844
TAATAGG700.0186.6842744
GGTTAAT1150.0164.1378544
CGTTAGC402.115452E-6145.1988744
ACGACAC1700.0145.1988744
TTACGAG2100.0145.1988544
ACATTGG4150.0136.4519544
ACTTCTA10950.0124.6456144
ACACTAC2250.0122.6123744
TAACCGA753.0431693E-9116.1590944
ATACGTG550.0114.6024443
CGTATTT500.0113.4564143
GATACTA451.8189894E-12112.05572543
TGGTACG451.8189894E-12112.05572543
ATTAACT1450.0110.1508644
TATTCCC1200.0108.8991544
TAGAGTA708.352072E-8103.7134844
CATAGAA3300.0101.1992144
GCAATTC251.296152E-5100.8501443
Produced by FastQC (version 0.11.5)