##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840949.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4467142
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.962031428595736	39.0	37.0	39.0	32.0	39.0
2	37.59261894965506	39.0	37.0	39.0	35.0	39.0
3	37.83916338455325	39.0	37.0	39.0	35.0	40.0
4	38.45016881039376	39.0	38.0	41.0	35.0	41.0
5	38.98927927520549	40.0	38.0	41.0	35.0	41.0
6	38.96971889409381	40.0	38.0	41.0	35.0	41.0
7	38.956439486365106	40.0	38.0	41.0	35.0	41.0
8	38.84294544476088	40.0	38.0	41.0	35.0	41.0
9	38.6723338993925	40.0	38.0	41.0	35.0	41.0
10	38.53979882439376	40.0	38.0	41.0	35.0	41.0
11	38.23346269270151	40.0	37.0	41.0	34.0	41.0
12	38.23217932181247	40.0	37.0	41.0	34.0	41.0
13	38.07565082999376	40.0	37.0	41.0	34.0	41.0
14	37.95763040440622	39.0	36.0	41.0	33.0	41.0
15	37.9412734137397	39.0	36.0	41.0	33.0	41.0
16	37.78842669429358	39.0	35.0	41.0	33.0	41.0
17	37.77006327535592	39.0	35.0	41.0	33.0	41.0
18	37.69169974896701	39.0	35.0	41.0	33.0	41.0
19	37.715625561041044	39.0	35.0	41.0	33.0	41.0
20	37.55393470814225	39.0	35.0	41.0	33.0	41.0
21	37.47186075571361	39.0	35.0	41.0	33.0	41.0
22	37.20859063804106	38.0	35.0	41.0	33.0	41.0
23	37.066835797921804	38.0	35.0	41.0	33.0	41.0
24	36.73759217862338	37.0	35.0	40.0	33.0	41.0
25	36.65033142983921	37.0	35.0	40.0	33.0	41.0
26	36.46191561835766	36.0	35.0	40.0	33.0	41.0
27	36.34927603077451	36.0	35.0	40.0	32.0	41.0
28	36.20873645101376	36.0	35.0	40.0	32.0	41.0
29	36.10671401480403	35.0	35.0	40.0	32.0	41.0
30	35.96621293416525	35.0	35.0	39.0	32.0	41.0
31	35.76090178918877	35.0	35.0	39.0	32.0	41.0
32	35.62840242337817	35.0	34.0	39.0	31.0	41.0
33	35.53009950325849	35.0	34.0	39.0	31.0	41.0
34	35.42063806332001	35.0	34.0	39.0	31.0	41.0
35	35.28718418789513	35.0	34.0	38.0	31.0	41.0
36	35.15280178058678	35.0	34.0	37.0	31.0	40.0
37	35.107940130233125	35.0	34.0	37.0	31.0	40.0
38	35.0243899366533	35.0	34.0	37.0	31.0	40.0
39	34.9491593186976	35.0	34.0	37.0	31.0	40.0
40	34.90370224833423	35.0	34.0	36.0	31.0	39.0
41	34.83359024275474	35.0	34.0	36.0	31.0	39.0
42	34.71153254832713	35.0	34.0	36.0	31.0	39.0
43	34.560535094884145	35.0	34.0	36.0	31.0	39.0
44	34.38157725065585	35.0	34.0	35.0	31.0	39.0
45	34.214536390670766	35.0	34.0	35.0	31.0	38.0
46	33.97503958295801	35.0	33.0	35.0	30.0	37.0
47	33.857854357083085	35.0	33.0	35.0	30.0	37.0
48	33.80854552810459	35.0	33.0	35.0	29.0	37.0
49	33.833516998399	35.0	33.0	35.0	30.0	37.0
50	34.00193648078872	35.0	33.0	36.0	30.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	17.0
21	47.0
22	138.0
23	313.0
24	720.0
25	1607.0
26	3395.0
27	7275.0
28	15182.0
29	29098.0
30	52278.0
31	90628.0
32	149795.0
33	252171.0
34	459436.0
35	497007.0
36	747399.0
37	798347.0
38	615318.0
39	534283.0
40	212687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.232201707489935	19.17407147567729	39.59372681683278
2	27.531607457295966	17.68007374737584	29.070130298074247	25.718188497253948
3	25.054050218237972	17.201378420475553	29.46850581423201	28.27606554705447
4	22.517529104738557	18.37649217329559	33.18396863139788	25.922010090567976
5	22.827973679815862	19.987029738477087	30.68171999009658	26.503276591610476
6	24.036263006638247	21.68099872356867	27.430670437608658	26.852067832184424
7	30.12563737620161	18.01265775746551	21.719479703130098	30.14222516320278
8	27.64745333817461	20.921878015070934	22.029230322206008	29.401438324548444
9	28.2148631048666	19.85258135962546	25.021523828882092	26.91103170662585
10	31.986693057888022	17.178679343526575	23.61521079920898	27.219416799376422
11	29.275854673972756	19.424432892439956	22.96754837880685	28.33216405478044
12	30.725237747087515	19.82204729556392	22.107736893073916	27.34497806427465
13	31.306481862452546	17.673805757685788	24.219176377200455	26.800536002661207
14	25.454664302142177	20.412088982172495	21.286988414516486	32.846258301168845
15	26.806468207189294	22.209412640117552	22.08488111638269	28.899238036310464
16	27.83354547493677	22.610228195118935	19.393294415086874	30.16293191485742
17	29.21601775811022	24.76406615236319	17.279884991343458	28.740031098183135
18	26.776449013709435	20.23710014143271	19.055293071050798	33.93115777380706
19	30.28840363704579	19.523086573025886	20.40064990098815	29.787859888940176
20	28.415170146818703	20.159197983856345	23.264337690630832	28.161294178694117
21	30.05769684509693	24.446301460754995	18.162126925895798	27.333874768252276
22	30.79969251033435	21.412258665607673	22.181743942771465	25.606304881286512
23	32.63661643171406	21.401961253974015	20.192082544051658	25.76933977026027
24	30.610533535759554	21.8150665459034	23.1174204894315	24.45697942890555
25	31.407404935586563	23.823107848908847	19.42434315473768	25.345144060766913
26	31.831719598925222	23.988310041233447	20.72734798186191	23.452622377979417
27	30.161993189607035	24.452837298763107	20.597401210979843	24.78776830065002
28	29.853337306303374	21.915586314723914	20.367802518615267	27.863273860357445
29	30.937262794855585	24.762411173200345	19.07149554893814	25.228830483005925
30	31.469697272538383	23.83153112407563	19.79614110454828	24.902630498837702
31	29.70645158947502	23.65848327190848	20.05817692466824	26.576888213948262
32	28.53438670740146	22.085725099723096	23.28389160850816	26.09599658436729
33	25.877045450048474	21.063465957460505	23.20340941517907	29.85607917731195
34	30.22633717169601	22.467108329865322	23.877468737415434	23.429085761023238
35	27.0774015665675	20.682182231622658	21.290055163009793	30.95036103880005
36	26.093076348627587	23.146702271904957	26.28205050112505	24.478170878342407
37	25.5299395426784	25.62947562947563	24.313853294745012	24.52673153310096
38	26.96562244813776	22.24186454585559	21.909470981204464	28.883042024802187
39	28.661433151269893	22.52033721791735	22.030049673116757	26.788179957696002
40	27.052936948604778	22.558348528393125	22.49857906560156	27.89013545740054
41	27.31889530213737	22.577164534609405	20.817025087565508	29.286915075687713
42	25.4184311018624	25.270633206298253	21.5873218393591	27.723613852480245
43	27.24986960843166	21.675766021329835	22.866485767248847	28.207878602989656
44	28.712601812489563	23.61735309723476	19.99850438959013	27.671540700685547
45	27.43475756023934	20.81929545577058	24.64658239448008	27.09936458951
46	26.321239809291335	23.920666617610863	21.966765539482733	27.791328033615066
47	27.204219253101503	21.52610435736464	23.69583326607325	27.573843123460605
48	24.30445099713928	23.798158082753332	20.056889208553276	31.840501711554115
49	27.737607268404567	24.546208672671572	19.749503315650344	27.96668074327352
50	23.99453228953772	26.27324024578283	25.53364656006392	24.198580904615525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77649.0
1	55728.0
2	39152.0
3	40456.5
4	31282.5
5	20986.5
6	12127.0
7	9567.0
8	11702.5
9	13253.5
10	13652.0
11	12974.5
12	12039.5
13	11225.5
14	10847.5
15	10500.5
16	10026.5
17	9867.0
18	9913.0
19	10831.5
20	12034.0
21	12911.5
22	13545.0
23	14632.0
24	16517.5
25	17721.0
26	18162.0
27	18467.0
28	19291.5
29	22218.5
30	26022.5
31	33639.5
32	46541.5
33	57074.5
34	68154.5
35	77178.5
36	80890.0
37	80172.0
38	77525.5
39	86439.5
40	98884.5
41	107200.0
42	115590.0
43	114026.5
44	107338.5
45	112847.0
46	127100.0
47	142918.0
48	164561.5
49	201128.5
50	244067.0
51	265111.5
52	265786.5
53	255453.0
54	255963.5
55	273857.0
56	287350.5
57	302327.5
58	313380.0
59	310561.0
60	310392.0
61	319085.5
62	322111.0
63	315995.0
64	298381.0
65	282244.0
66	273786.5
67	270857.5
68	283545.5
69	290677.5
70	256536.5
71	213754.5
72	199889.5
73	193664.0
74	173278.0
75	143237.5
76	114285.5
77	84343.0
78	63532.0
79	55694.0
80	48454.5
81	35147.5
82	24804.0
83	20645.0
84	18564.5
85	16118.5
86	12216.0
87	9208.5
88	5505.0
89	2656.5
90	1716.0
91	1021.0
92	578.0
93	434.0
94	256.5
95	65.5
96	37.0
97	37.0
98	18.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	31965.0
25	28318.0
26	22971.0
27	24429.0
28	26448.0
29	30329.0
30	34323.0
31	38396.0
32	35226.0
33	32893.0
34	31540.0
35	26039.0
36	26347.0
37	29404.0
38	29036.0
39	29146.0
40	37577.0
41	33890.0
42	57419.0
43	77038.0
44	222616.0
45	408920.0
46	489083.0
47	572707.0
48	696333.0
49	789011.0
50	605738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.405806024341292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95340107752159	9.298573546323862
2	10.050850071444186	2.4937779273214673
3	3.430460920457068	1.2767289825982098
4	1.6762617961537822	0.8318151475639098
5	1.005459020882765	0.6236764789247851
6	0.7078416496377024	0.5268807720813051
7	0.512970425608345	0.44546681174246455
8	0.3772868340117276	0.3744437822629874
9	0.3419871987655741	0.38183641656242057
>10	3.3722966908642316	9.650675956584799
>50	1.597407849568243	14.572404085339317
>100	1.8629701338165594	40.58288327415646
>500	0.07149794000822433	6.014442080590713
>1k	0.03528469766639642	8.09729327106259
>5k	0.0024761191344839593	1.9941138351665813
>10k+	0.0015475744590524745	2.8349876317181564
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	20896	0.46777111629762386	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17038	0.38140717263968776	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG	13368	0.2992517363450725	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	12082	0.2704637551257605	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10448	0.23388555814881193	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	8751	0.19589706349160155	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7186	0.1608634782597016	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	6531	0.1462008595204719	No Hit
TCAGAACTGCGTCTCGCGACCCAGGCGCGGG	6115	0.13688841769525123	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	6110	0.13677648930792888	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA	6094	0.1364183184684973	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	5896	0.1319859543305317	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	5250	0.11752480668848225	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4810	0.10767510860411422	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	4560	0.10207868923799601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.010879439247733786	0.0	0.0	0.0	0.0
2	0.023370647272909613	0.0	0.0	0.0	0.0
3	0.033645673229102634	0.0	0.0	0.0	0.0
4	0.04510714009091271	0.0	0.0	0.0	0.0
5	0.0562328217907557	0.0	0.0	0.0	0.0
6	0.07082828349759197	0.0	0.0	0.0	0.0
7	0.08146148029321655	0.0	0.0	0.0	0.0
8	0.09325873231699373	0.0	0.0	0.0	0.0
9	0.10230254601264074	0.0	0.0	0.0	0.0
10	0.11080910344914041	0.0	0.0	0.0	0.0
11	0.11920373249831771	0.0	0.0	0.0	0.0
12	0.12838186025875156	0.0	0.0	0.0	0.0
13	0.1380748586008683	0.0	0.0	0.0	0.0
14	0.14839465591199025	0.0	0.0	0.0	0.0
15	0.15772948341467544	0.0	0.0	0.0	0.0
16	0.16791496666101055	0.0	0.0	0.0	0.0
17	0.18067480281576007	0.0	0.0	0.0	8.954270985789124E-5
18	0.20366489357177364	0.0	0.0	0.0	8.954270985789124E-5
19	0.21526067449837055	0.0	0.0	0.0	8.954270985789124E-5
20	0.22553570045456356	0.0	0.0	0.0	8.954270985789124E-5
21	0.2357211837008987	0.0	0.0	0.0	8.954270985789124E-5
22	0.2444292122345786	0.0	0.0	0.0	8.954270985789124E-5
23	0.25618169290342685	0.0	0.0	0.0	8.954270985789124E-5
24	0.267598388410308	0.0	0.0	0.0	8.954270985789124E-5
25	0.28123126598617193	0.0	0.0	0.0	8.954270985789124E-5
26	0.2960729701451174	0.0	0.0	0.0	8.954270985789124E-5
27	0.30697479507031566	0.0	0.0	0.0	8.954270985789124E-5
28	0.31913021793352436	0.0	0.0	0.0	8.954270985789124E-5
29	0.3317333543460226	0.0	0.0	0.0	8.954270985789124E-5
30	0.34509760379231286	0.0	0.0	0.0	8.954270985789124E-5
31	0.35819322510902946	0.0	0.0	0.0	8.954270985789124E-5
32	0.3706396617792763	0.0	0.0	0.0	8.954270985789124E-5
33	0.38333234090163243	0.0	0.0	0.0	8.954270985789124E-5
34	0.3938536093099346	0.0	0.0	0.0	8.954270985789124E-5
35	0.4030988941027619	0.0	0.0	0.0	8.954270985789124E-5
36	0.4118964653462997	0.0	0.0	0.0	8.954270985789124E-5
37	0.4181644550363521	0.0	0.0	0.0	8.954270985789124E-5
38	0.4233355465306453	0.0	0.0	0.0	8.954270985789124E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	4835	0.0	220.7263	44
TCGTTAC	15	0.0029866376	193.59848	44
TAATAGG	70	0.0	186.68427	44
GGTTAAT	115	0.0	164.13785	44
CGTTAGC	40	2.115452E-6	145.19887	44
ACGACAC	170	0.0	145.19887	44
TTACGAG	210	0.0	145.19885	44
ACATTGG	415	0.0	136.45195	44
ACTTCTA	1095	0.0	124.64561	44
ACACTAC	225	0.0	122.61237	44
TAACCGA	75	3.0431693E-9	116.15909	44
ATACGTG	55	0.0	114.60244	43
CGTATTT	50	0.0	113.45641	43
GATACTA	45	1.8189894E-12	112.055725	43
TGGTACG	45	1.8189894E-12	112.055725	43
ATTAACT	145	0.0	110.15086	44
TATTCCC	120	0.0	108.89915	44
TAGAGTA	70	8.352072E-8	103.71348	44
CATAGAA	330	0.0	101.19921	44
GCAATTC	25	1.296152E-5	100.85014	43
>>END_MODULE