FastQCFastQC Report
Tue 24 May 2016
ERR840948.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840948.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815461
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC250280.36722387524482936No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA218040.3199196650087206No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG184520.2707373719840815No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC119950.17599689881579544No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG94830.13913952409088687No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGG93360.13698266338843404No Hit
AGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCA80680.11837790576455502No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA80400.117967075154564No Hit
AGCTATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA71110.1043363024159334No Hit
AGCTATTGTGGCTCCGGCCGGTTGCGCGGG68520.10053611927351651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT152.6608079E-5212.8977544
CTATCAT33000.0162.5764544
TAAGCCA21550.0111.1415244
CCTATCA40450.082.2399543
ACCGATG901.9776053E-870.9659144
TTAAGCC20650.070.5349943
TCACGGA15100.069.19569442
TCACCGT6950.068.37833443
CAGGGTA7050.068.2838743
TATCATA8650.067.6842644
TCCGCAC5350.067.6497544
AACGACA5100.064.1384343
GGTACGA501.3423825E-463.8693244
CCCTATC51450.063.80400542
TGCACGA2200.062.90160844
TCAGAGA76400.060.21571742
AGGCCGA113300.058.49789442
CGATCTA550.004667239758.06302344
GTACGAT1301.8018909E-857.31862344
CCTATAG3000.056.77273644
Produced by FastQC (version 0.11.5)