##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840948.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6815461
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.28806195208219	39.0	35.0	39.0	32.0	39.0
2	36.950607009562525	39.0	35.0	39.0	33.0	39.0
3	37.075950695044696	39.0	35.0	39.0	33.0	39.0
4	37.542649426062304	39.0	35.0	39.0	34.0	41.0
5	38.26847457567434	40.0	35.0	41.0	35.0	41.0
6	38.28692820632383	40.0	35.0	41.0	35.0	41.0
7	38.31015730263881	40.0	35.0	41.0	35.0	41.0
8	38.22557461630255	40.0	35.0	41.0	35.0	41.0
9	38.11278415355909	40.0	35.0	41.0	34.0	41.0
10	38.01594345562244	40.0	35.0	41.0	34.0	41.0
11	37.67674130920858	40.0	35.0	41.0	33.0	41.0
12	37.74655375476435	39.0	35.0	41.0	33.0	41.0
13	37.62937239315139	39.0	35.0	41.0	33.0	41.0
14	37.61417547543739	39.0	35.0	41.0	33.0	41.0
15	37.610251016035456	39.0	35.0	41.0	33.0	41.0
16	37.46792799489279	39.0	35.0	41.0	33.0	41.0
17	37.502232350827036	39.0	35.0	41.0	33.0	41.0
18	37.46271998328506	39.0	35.0	41.0	33.0	41.0
19	37.51608467864463	39.0	35.0	41.0	33.0	41.0
20	37.44616937871114	39.0	35.0	41.0	33.0	41.0
21	37.41503707526167	39.0	35.0	41.0	33.0	41.0
22	36.99004528086948	38.0	35.0	41.0	33.0	41.0
23	37.05149512263367	38.0	35.0	41.0	33.0	41.0
24	36.82044941640778	37.0	35.0	41.0	33.0	41.0
25	36.81721898763407	37.0	35.0	41.0	33.0	41.0
26	36.70485542323357	37.0	35.0	40.0	33.0	41.0
27	36.61901195503707	36.0	35.0	40.0	33.0	41.0
28	36.49493211449924	36.0	35.0	40.0	33.0	41.0
29	36.38149631456048	35.0	35.0	40.0	33.0	41.0
30	36.32762354411315	35.0	35.0	40.0	33.0	41.0
31	36.03990312269185	35.0	35.0	40.0	32.0	41.0
32	36.05318052872859	35.0	35.0	40.0	33.0	41.0
33	35.984587028387296	35.0	35.0	40.0	33.0	41.0
34	35.905290827584466	35.0	35.0	39.0	32.0	41.0
35	35.80068776757372	35.0	35.0	39.0	32.0	41.0
36	35.644702587720545	35.0	35.0	39.0	32.0	41.0
37	35.68739848607037	35.0	35.0	39.0	32.0	41.0
38	35.52530445209871	35.0	35.0	39.0	32.0	41.0
39	35.49554907140681	35.0	35.0	39.0	32.0	41.0
40	35.53081885070448	35.0	35.0	38.0	32.0	41.0
41	35.51038504481397	35.0	35.0	38.0	32.0	41.0
42	35.37674876883105	35.0	34.0	37.0	32.0	41.0
43	35.30916165587863	35.0	34.0	37.0	32.0	41.0
44	35.10451661715183	35.0	34.0	37.0	31.0	41.0
45	35.08403860859492	35.0	34.0	37.0	31.0	41.0
46	34.85223879683247	35.0	34.0	37.0	31.0	40.0
47	34.78381365063863	35.0	34.0	37.0	31.0	40.0
48	34.47570038234372	35.0	33.0	37.0	29.0	40.0
49	34.908791696973395	35.0	34.0	37.0	31.0	40.0
50	35.22513635088177	35.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	22.0
21	84.0
22	286.0
23	716.0
24	1760.0
25	3967.0
26	8179.0
27	16216.0
28	30538.0
29	54262.0
30	92636.0
31	156083.0
32	262490.0
33	501770.0
34	1067592.0
35	495983.0
36	759352.0
37	930870.0
38	844478.0
39	846683.0
40	741487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.20861875667691	16.75274790656127	36.03863333676181
2	30.113634279471334	17.840847449644272	27.55932430689575	24.486193963988644
3	26.697900552875293	17.018936796791884	27.785970750914725	28.4971918994181
4	23.95160063273783	17.38451734959675	30.383784750584002	28.280097267081423
5	23.61554119376518	24.54608719791662	28.066362642233596	23.772008966084613
6	22.642532911566803	22.641696577825034	28.597522603386622	26.118247907221537
7	30.205278850542904	19.946941226719662	24.34984221903698	25.49793770370045
8	29.915540562846736	21.15291980982651	21.726307288677905	27.205232338648845
9	27.652685563016206	20.729881661710046	27.184910895976078	24.43252187929767
10	33.13420764934316	18.98829734334919	22.46528884839925	25.4122061589084
11	32.004174038997505	19.417483278093734	24.794360939047262	23.783981743861492
12	30.399044173240814	20.221552144455085	22.04041663505961	27.338987047244494
13	29.27231188029687	18.290134739234805	28.0881513370849	24.34940204338342
14	23.929327744667603	19.58363491479153	22.526047761112565	33.9609895794283
15	24.79797037940647	22.527793791205024	24.99436208350396	27.679873745884542
16	28.66371034916053	24.084753767940274	20.37156400718895	26.87997187571024
17	30.17412908679252	26.316004155845068	18.943751567208732	24.56611519015368
18	25.195287596833143	21.126553288178158	19.119190910196686	34.558968204792016
19	29.198421060585627	20.13891650175975	20.023751878266193	30.638910559388428
20	29.573171939506366	19.850440050937127	22.23318422627611	28.343203783280398
21	30.768689014580232	22.683219814477702	19.178893401341448	27.369197769600618
22	28.180280101375388	21.418771232056056	26.750677613737356	23.650271052831204
23	30.538697235594185	22.026389704232773	24.179963761805695	23.254949298367343
24	32.39446311848898	21.70494409695837	23.337482233410185	22.56311055114247
25	28.577599853678983	25.24346331093556	20.095324360927737	26.083612474457716
26	32.395784544717515	24.582210947511573	21.196969498843337	21.825035008927575
27	27.836252961584655	23.14949858363539	22.487811739073564	26.526436715706385
28	30.706748265151653	21.79822656277439	20.205861366427406	27.289163805646556
29	30.889612221629648	23.814178190536673	20.665617745298082	24.630591842535598
30	31.970418214760443	22.86232383943869	19.749766456391317	25.41749148940955
31	26.652813916497337	25.131202706802863	20.910098103215248	27.30588527348455
32	28.48619145478168	21.84841660532829	22.369756803620035	27.29563513627
33	24.82791357489212	21.231792248941574	22.710753132460233	31.229541043706078
34	27.285040293655715	23.49706521383088	26.03028618455543	23.187608307957973
35	25.12841564781753	20.848988755884353	22.13542674035906	31.887168855939063
36	28.85301975378957	22.047934304073323	25.748984420501454	23.350061521635652
37	28.17083893274842	24.031393659224214	24.731647183602647	23.066120224424715
38	26.01369668854207	25.385846090951077	23.879149285741605	24.721307934765246
39	28.33260446005704	23.334033612765328	21.02052185600162	27.312840071176016
40	27.37217260360938	22.859282902422567	21.6769160286297	28.091628465338353
41	28.211853766369167	22.784393958251243	21.25129520976946	27.75245706561013
42	24.81397798932315	25.954730127634427	24.213925642749174	25.01736624029325
43	27.266802358289006	22.541374172403838	22.286655982996965	27.90516748631019
44	27.319602534152647	26.045399202523672	21.453786889913772	25.181211373409905
45	28.200113609572774	22.197699316440698	22.62244338091467	26.97974369307186
46	26.723703619294238	24.803135222052884	21.65732760791397	26.815833550738905
47	30.891757622574744	22.176536877292197	21.907568365599666	25.024137134533397
48	23.657499466934283	28.08290628288614	21.8005941064025	26.45900014377708
49	27.523120353284643	25.133660630810812	21.566813018950544	25.776405996954004
50	24.50189386049648	28.675932526847703	24.980723416195787	21.841450196460023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89438.0
1	73251.0
2	58247.5
3	55179.5
4	46737.0
5	34993.0
6	23824.0
7	20658.0
8	22222.0
9	24346.0
10	25350.5
11	24141.0
12	21585.5
13	19867.5
14	20413.0
15	21560.0
16	21232.0
17	19812.5
18	19919.0
19	22481.5
20	25858.5
21	32551.5
22	39684.5
23	43797.5
24	44917.5
25	45963.5
26	52399.5
27	61870.0
28	68742.0
29	75919.0
30	85524.5
31	94306.0
32	103201.5
33	113400.0
34	126070.0
35	134579.5
36	136839.0
37	136091.0
38	136211.5
39	148088.5
40	165373.0
41	182675.5
42	194990.5
43	204219.0
44	211789.5
45	207117.5
46	210703.0
47	229232.0
48	258396.5
49	298084.5
50	335305.5
51	360540.0
52	371926.0
53	364200.5
54	363734.0
55	387814.5
56	409564.0
57	422827.5
58	421927.5
59	407483.0
60	404803.5
61	414929.0
62	414228.5
63	402110.0
64	387365.5
65	375198.5
66	368869.5
67	370564.5
68	428757.5
69	487210.0
70	457054.0
71	409529.0
72	368911.5
73	314536.5
74	254417.5
75	198280.0
76	151791.0
77	109664.0
78	83778.5
79	70461.0
80	57720.0
81	42940.0
82	30774.0
83	23540.0
84	19038.0
85	16147.5
86	12762.0
87	9643.5
88	6341.0
89	3786.5
90	2503.0
91	1456.5
92	973.0
93	841.5
94	540.5
95	248.5
96	196.0
97	190.0
98	131.5
99	72.5
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	46782.0
25	38967.0
26	32284.0
27	34886.0
28	37959.0
29	43371.0
30	55432.0
31	48052.0
32	54502.0
33	42693.0
34	46998.0
35	37376.0
36	32856.0
37	40485.0
38	44332.0
39	38429.0
40	41624.0
41	46461.0
42	63393.0
43	109533.0
44	305572.0
45	509973.0
46	851112.0
47	727828.0
48	1287579.0
49	924713.0
50	1272269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.078252249312087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35794711930927	15.732906279997488
2	10.079474403072492	4.047564592107477
3	3.208855374719008	1.9328462293550734
4	1.389662426061575	1.1160797092742125
5	0.834896901286309	0.8381635293097761
6	0.5443393185413165	0.6557629288134716
7	0.4009817731648304	0.5635709232285933
8	0.30680134879167403	0.4928027897174728
9	0.23884191351740483	0.43159753685797525
>10	2.6199902354442215	12.6226525230753
>50	1.171157439880061	17.09225879125694
>100	0.7924695498595437	26.568742324849946
>500	0.03379985380813341	4.569599161361408
>1k	0.017585059075853195	6.472685549339759
>5k	0.0022837739059549606	3.319201293405317
>10k+	9.135095623819841E-4	3.5435658380497537
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	25028	0.36722387524482936	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	21804	0.3199196650087206	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	18452	0.2707373719840815	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	11995	0.17599689881579544	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGG	9483	0.13913952409088687	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGG	9336	0.13698266338843404	No Hit
AGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCA	8068	0.11837790576455502	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	8040	0.117967075154564	No Hit
AGCTATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	7111	0.1043363024159334	No Hit
AGCTATTGTGGCTCCGGCCGGTTGCGCGGG	6852	0.10053611927351651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.021803367373094792	0.0	0.0	0.0	0.0
2	0.0408483006505356	0.0	0.0	0.0	0.0
3	0.05521269947843587	0.0	0.0	0.0	0.0
4	0.06853534925957319	0.0	0.0	0.0	0.0
5	0.08455774304922294	0.0	0.0	1.4672521785393534E-5	0.0
6	0.10684530364123572	0.0	0.0	0.0022155507895944234	0.0
7	0.12173791325341016	0.0	0.0	0.0022155507895944234	0.0
8	0.13564746390596322	0.0	0.0	0.0022155507895944234	0.0
9	0.1495570145585163	0.0	0.0	0.0022155507895944234	0.0
10	0.1615444648571828	0.0	0.0	0.0022155507895944234	0.0
11	0.17351724263406393	0.0	0.0	0.0022155507895944234	0.0
12	0.18553403797630125	0.0	0.0	0.0022155507895944234	0.0
13	0.19863659993065766	0.0	0.0	0.0022155507895944234	0.0
14	0.2103892898807579	0.0	0.0	0.0022155507895944234	0.0
15	0.22319840139940644	0.0	0.0	0.0022155507895944234	0.0
16	0.2348337111752235	0.0	0.0	0.0022155507895944234	0.0
17	0.2503425667023845	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
18	0.27085475215836463	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
19	0.2809494471467154	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
20	0.2923793416175369	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
21	0.3055259211372496	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
22	0.3181736349162588	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
23	0.3303958455634916	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
24	0.34255936612358284	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
25	0.3557939807740078	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
26	0.36913130307693054	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
27	0.38158827407272966	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
28	0.39570324003027824	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
29	0.4095687731174751	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
30	0.42462278046928886	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
31	0.43967678782110264	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
32	0.45415856682328604	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
33	0.4667916080805099	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
34	0.4791898889891674	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
35	0.48922589389037663	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
36	0.4998194546194307	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
37	0.5073464582953376	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
38	0.5142718885780434	0.0	0.0	0.0022155507895944234	4.40175653561806E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGT	15	2.6608079E-5	212.89775	44
CTATCAT	3300	0.0	162.57645	44
TAAGCCA	2155	0.0	111.14152	44
CCTATCA	4045	0.0	82.23995	43
ACCGATG	90	1.9776053E-8	70.96591	44
TTAAGCC	2065	0.0	70.53499	43
TCACGGA	1510	0.0	69.195694	42
TCACCGT	695	0.0	68.378334	43
CAGGGTA	705	0.0	68.28387	43
TATCATA	865	0.0	67.68426	44
TCCGCAC	535	0.0	67.64975	44
AACGACA	510	0.0	64.13843	43
GGTACGA	50	1.3423825E-4	63.86932	44
CCCTATC	5145	0.0	63.804005	42
TGCACGA	220	0.0	62.901608	44
TCAGAGA	7640	0.0	60.215717	42
AGGCCGA	11330	0.0	58.497894	42
CGATCTA	55	0.0046672397	58.063023	44
GTACGAT	130	1.8018909E-8	57.318623	44
CCTATAG	300	0.0	56.772736	44
>>END_MODULE