FastQCFastQC Report
Tue 24 May 2016
ERR840947.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840947.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4119357
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA560741.361231862157128No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC320610.7783010795131377No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG230610.5598203797340215No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG115190.2796310200839597No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC92480.2245010568396961No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA82080.19925439819855378No Hit
CCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA80000.19420506647032534No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC73710.17893569311909602No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGG72620.17628964908843783No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68240.1656569216991875No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGG60030.14572662675267037No Hit
CCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC49980.12132961527733575No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC46070.11183784265359861No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC42490.10314716592905156No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA42440.1030257877625076No Hit
CCGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG42230.10251599946302298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA152.0429563E-5227.4671244
GACGAAT100.0018435293227.4671244
CTATCAT18000.0170.6003444
GTCATAC650.0157.4772344
GCTAATC355.678885E-9106.2046943
GACGTCG905.456968E-12101.096544
GCGGATA251.4121404E-599.1243943
CTTACGA1300.096.23608444
GCACTAT604.683734E-794.7779644
AAACTAC5300.091.17573543
GTTTGAT701.1722077E-681.2382544
AATACGG150.001395987878.7281542
CGATATA351.4661055E-978.7281442
ACTTGTC2900.078.43693544
CGTATGA1750.077.9887244
GTACACT905.885704E-875.8223744
TCTAAGA1650.075.8223744
AGATACG2300.074.1740644
ATAACGA700.073.10470642
CCTATCA23000.072.7271343
Produced by FastQC (version 0.11.5)