##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840947.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4119357 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.255484047631704 39.0 37.0 39.0 34.0 39.0 2 37.77375182583107 39.0 38.0 39.0 35.0 39.0 3 37.91537538504189 39.0 38.0 39.0 35.0 40.0 4 38.38890729791081 39.0 38.0 40.0 35.0 41.0 5 39.04611714886571 40.0 38.0 41.0 35.0 41.0 6 38.92093401955694 40.0 38.0 41.0 35.0 41.0 7 38.97229324867934 40.0 38.0 41.0 35.0 41.0 8 38.828840763255045 40.0 38.0 41.0 35.0 41.0 9 38.77926312286117 40.0 38.0 41.0 35.0 41.0 10 38.74715277165829 40.0 38.0 41.0 35.0 41.0 11 38.08921076760281 40.0 37.0 41.0 33.0 41.0 12 38.44304293121475 40.0 38.0 41.0 34.0 41.0 13 38.46054105046006 40.0 38.0 41.0 34.0 41.0 14 38.33493285481205 40.0 38.0 41.0 34.0 41.0 15 38.378693567952475 40.0 38.0 41.0 34.0 41.0 16 38.31073004840319 40.0 37.0 41.0 34.0 41.0 17 38.31439494076381 40.0 37.0 41.0 34.0 41.0 18 38.40186198962605 40.0 38.0 41.0 34.0 41.0 19 38.518062891854235 40.0 38.0 41.0 35.0 41.0 20 38.50766806567142 40.0 37.0 41.0 35.0 41.0 21 38.494447313015115 40.0 37.0 41.0 35.0 41.0 22 38.29672592106001 40.0 37.0 41.0 34.0 41.0 23 38.21857246167302 40.0 37.0 41.0 34.0 41.0 24 37.94795231391695 40.0 36.0 41.0 33.0 41.0 25 37.95176491878488 40.0 36.0 41.0 34.0 41.0 26 37.94978018041409 39.0 36.0 41.0 33.0 41.0 27 37.86034503073777 39.0 36.0 41.0 33.0 41.0 28 37.73048314076383 39.0 35.0 41.0 33.0 41.0 29 37.64001331785573 39.0 35.0 41.0 33.0 41.0 30 37.58571848035464 39.0 35.0 41.0 33.0 41.0 31 37.30873325681231 39.0 35.0 41.0 33.0 41.0 32 37.255339627631884 39.0 35.0 41.0 33.0 41.0 33 37.20080052204388 38.0 35.0 41.0 33.0 41.0 34 37.102758039543104 38.0 35.0 41.0 33.0 41.0 35 36.960667522397856 38.0 35.0 40.0 33.0 41.0 36 36.904792656354914 38.0 35.0 40.0 33.0 41.0 37 36.91324327618739 38.0 35.0 40.0 33.0 41.0 38 36.808417067611316 37.0 35.0 40.0 33.0 41.0 39 36.71525419116191 37.0 35.0 40.0 33.0 41.0 40 36.695618216808676 37.0 35.0 40.0 33.0 41.0 41 36.58934339220234 37.0 35.0 40.0 33.0 41.0 42 36.382827606976555 36.0 35.0 40.0 32.0 41.0 43 36.32565002049912 36.0 35.0 40.0 32.0 41.0 44 36.18621959836615 36.0 35.0 40.0 32.0 41.0 45 36.038882876607246 36.0 34.0 40.0 31.0 41.0 46 35.783842471580584 35.0 34.0 40.0 31.0 41.0 47 35.681067430103326 35.0 34.0 39.0 31.0 41.0 48 35.33700559532602 35.0 34.0 39.0 29.0 41.0 49 35.80283148310784 36.0 34.0 40.0 31.0 41.0 50 35.95075094456694 37.0 34.0 39.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 6.0 21 22.0 22 65.0 23 157.0 24 393.0 25 837.0 26 1639.0 27 3467.0 28 7320.0 29 15089.0 30 29087.0 31 53993.0 32 93063.0 33 160989.0 34 288558.0 35 279966.0 36 486587.0 37 599270.0 38 615116.0 39 792360.0 40 691372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 46.18502353644027 16.158492696797097 37.656483766762626 2 27.80756802578655 19.407519183212333 27.714640901480497 25.070271889520622 3 29.033754539846875 16.474707096277406 26.64796957389224 27.843568789983486 4 26.579002499661964 19.74317836497298 30.068139275134442 23.609679860230614 5 27.45962537357165 19.80442578781106 27.231215939769243 25.504732898848047 6 21.835276719157868 21.52969504706681 26.89718322544028 29.73784500833504 7 33.80245994702572 20.846166039991193 21.542682510887015 23.80869150209608 8 26.39421637891545 19.738274687044605 23.744676657060797 30.12283227697915 9 30.652866454643284 19.56271330695543 25.365026629155956 24.419393609245326 10 32.95613854298134 17.158745891652508 24.419976224444735 25.46513934092141 11 25.88670998896187 20.80783481499661 28.731401526985888 24.574053669055633 12 34.970676248744645 19.67646892464042 21.13252141050169 24.22033341611324 13 32.43219754927772 18.752829628507556 24.48515629987884 24.32981652233589 14 24.71133237541684 20.842379041195024 21.574750622487926 32.87153796090021 15 23.405837367336698 21.160681145139883 28.85319237929609 26.58028910822733 16 26.74385832546196 22.965186071515532 19.92281805145803 30.368137551564477 17 26.7917541499802 29.559127795915725 19.150124643239224 24.49899341086485 18 25.13569472128781 20.93489347973482 20.1141100419313 33.815301757046065 19 31.8989104367502 20.105225160140282 21.36110077373726 26.634763629372255 20 27.09415571410781 20.8975575557059 22.099104301957805 29.90918242822848 21 26.48136590249401 24.045014792357154 19.87970452670162 29.593914778447218 22 28.037385446320872 21.790439624436534 27.148630235252735 23.023544693989866 23 33.197341235537486 21.503331709293466 22.37259358681464 22.926733468354406 24 27.726608788701736 22.702305238414635 27.445958192018804 22.12512778086483 25 26.913091750343593 29.359669759411517 21.17124872222521 22.555989768019682 26 28.509890959355566 29.24553236760729 21.625005316926956 20.61957135611019 27 26.58822506463861 23.97726242547138 26.28812208255512 23.14639042733489 28 27.946328342243596 22.568162851404278 20.740769985029957 28.744738821322162 29 27.77616518134472 24.876536538869995 19.73538498861019 27.6119132911751 30 27.460074935868473 24.224851812398605 19.631067566111426 28.6840056856215 31 24.084756304135674 29.960584930751843 21.208568669191525 24.74609009592096 32 30.98370116260984 22.354636858481637 22.841257464710715 23.82040451419781 33 23.831575131858692 22.647965913122412 21.963989856905144 31.556469098113755 34 26.053702933034177 28.402947767849064 24.4661112676384 21.077238031478355 35 24.509199978129388 22.371868141023803 21.271984523936606 31.846947356910206 36 25.22660711661225 22.796155898675135 28.464169759572073 23.513067225140546 37 24.754692959119925 24.62400645745067 28.39422251930614 22.227078064123265 38 24.86631717705986 24.17150844163986 26.663908142743804 24.298266238556476 39 30.744614603071607 23.17333536819311 21.767734630093976 24.314315398641302 40 31.4331179751751 23.09361851106467 21.403982336048937 24.06928117771129 41 31.265158196822817 23.297590857251627 20.586379212728207 24.850871733197348 42 23.825087315982334 30.854926692730867 21.318999385415403 24.000986605871393 43 29.995255445492177 23.467883609079568 21.230614003764785 25.306246941663467 44 30.95661528172865 24.729208131562032 20.686718928901666 23.62745765780765 45 25.123755265189008 22.932915376099313 22.56823939661571 29.37508996209597 46 25.516402606393118 24.38362103688548 21.653870260619666 28.446106096101744 47 29.31422544297062 24.00454232254885 22.26208918585068 24.41914304862985 48 23.308158676970265 28.82656213628226 21.542963118362078 26.322316068385405 49 25.293203035975754 25.665412878052756 22.382883029149266 26.658501056822224 50 24.63505368242607 29.937232003368308 24.258750401648697 21.168963912556922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44920.0 1 40257.5 2 37261.0 3 36271.5 4 30267.5 5 20737.5 6 12392.0 7 10764.0 8 12222.0 9 13755.5 10 14117.0 11 14034.0 12 13733.5 13 13038.5 14 13413.0 15 13738.0 16 13568.0 17 14454.0 18 15473.5 19 15383.5 20 15387.5 21 17690.5 22 20947.5 23 25371.5 24 30267.0 25 34129.0 26 37806.0 27 41406.5 28 45032.0 29 48761.5 30 51001.0 31 56660.5 32 66760.5 33 70863.5 34 76953.0 35 86389.0 36 93153.5 37 98274.0 38 100921.0 39 108314.5 40 116803.0 41 130218.5 42 148273.0 43 147858.0 44 139363.0 45 142598.5 46 152249.0 47 167546.0 48 189402.0 49 200973.5 50 207903.0 51 218514.0 52 225415.5 53 230428.0 54 237403.0 55 240890.0 56 237760.0 57 237902.0 58 240446.0 59 236095.0 60 227049.5 61 226041.5 62 223902.5 63 212254.5 64 201147.5 65 205281.5 66 234114.0 67 266805.5 68 308313.5 69 321459.0 70 263138.5 71 191130.0 72 156006.5 73 150611.5 74 132158.0 75 103731.5 76 81051.5 77 57872.0 78 41741.5 79 33729.0 80 28931.5 81 22684.0 82 15500.5 83 11143.5 84 9349.0 85 7540.5 86 5232.0 87 3902.5 88 2781.5 89 1563.0 90 738.0 91 448.5 92 443.5 93 437.5 94 309.5 95 233.5 96 245.0 97 241.0 98 122.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 30217.0 25 21941.0 26 18922.0 27 20900.0 28 20726.0 29 25380.0 30 25931.0 31 27023.0 32 32859.0 33 26768.0 34 27920.0 35 23701.0 36 18666.0 37 25854.0 38 24543.0 39 23358.0 40 26154.0 41 29349.0 42 39721.0 43 61647.0 44 191355.0 45 322700.0 46 505246.0 47 460662.0 48 763561.0 49 602229.0 50 722024.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.420679443887023 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33791199731535 10.245066463316267 2 9.502854510486275 2.5506952837426446 3 3.355156174275324 1.350854264973824 4 1.573579795575893 0.8447404006320531 5 0.9345857770178505 0.6271388063086323 6 0.6488467963587777 0.5224778917274518 7 0.47140762512356793 0.44286274369312273 8 0.36914560747580794 0.3963347892841411 9 0.3071592487671797 0.37100572343365223 >10 3.1055707032931195 9.928449394942232 >50 1.6406197988698432 16.191093873299653 >100 1.6764975450529331 38.22177144650618 >500 0.0466119675959569 4.18226758064262 >1k 0.026679218295054274 6.897051913502065 >5k 0.002146603770866436 2.1777867891211935 >10k+ 0.0012266307262093921 5.050402634874278 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA 56074 1.361231862157128 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC 32061 0.7783010795131377 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG 23061 0.5598203797340215 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG 11519 0.2796310200839597 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC 9248 0.2245010568396961 No Hit AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA 8208 0.19925439819855378 No Hit CCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGA 8000 0.19420506647032534 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 7371 0.17893569311909602 No Hit AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGG 7262 0.17628964908843783 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6824 0.1656569216991875 No Hit AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGG 6003 0.14572662675267037 No Hit CCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC 4998 0.12132961527733575 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC 4607 0.11183784265359861 No Hit CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC 4249 0.10314716592905156 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 4244 0.1030257877625076 No Hit CCGGGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG 4223 0.10251599946302298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.023061851643351134 0.0 0.0 0.0 0.0 2 0.04507985105442427 0.0 0.0 0.0 0.0 3 0.05894123767374374 0.0 0.0 0.0 0.0 4 0.07103050306152149 0.0 0.0 0.0 0.0 5 0.0816875060840806 0.0 0.0 0.0 0.0 6 0.09957864783265932 0.0 0.0 0.0 0.0 7 0.11336720755205242 0.0 0.0 0.0 0.0 8 0.12557785110637412 0.0 0.0 0.0 0.0 9 0.13827400732687165 0.0 0.0 0.0 0.0 10 0.14919804231582745 0.0 0.0 0.0 0.0 11 0.1601949042047096 0.0 0.0 0.0 0.0 12 0.1717258300263852 0.0 0.0 0.0 0.0 13 0.18398502484732449 0.0 0.0 0.0 0.0 14 0.19605001460179344 0.0 0.0 0.0 0.0 15 0.20845486322258547 0.0 0.0 0.0 0.0 16 0.22173363464249396 0.0 0.0 0.0 0.0 17 0.23341021426402228 0.0 0.0 0.0 0.0 18 0.2523694838781878 0.0 0.0 0.0 0.0 19 0.264871435032215 0.0 0.0 0.0 0.0 20 0.27577119438786196 0.0 0.0 0.0 0.0 21 0.2897296835404166 0.0 0.0 0.0 0.0 22 0.30252294229414933 2.4275633308790667E-5 0.0 0.0 0.0 23 0.31720970044596764 2.4275633308790667E-5 0.0 0.0 0.0 24 0.33153232409815414 2.4275633308790667E-5 0.0 0.0 0.0 25 0.34512667875107694 2.4275633308790667E-5 0.0 0.0 0.0 26 0.3665620629627391 2.4275633308790667E-5 0.0 0.0 0.0 27 0.3805691033819113 2.4275633308790667E-5 0.0 0.0 0.0 28 0.39421200930145167 2.4275633308790667E-5 0.0 0.0 0.0 29 0.4082918766205502 2.4275633308790667E-5 0.0 0.0 0.0 30 0.42254167337281034 2.4275633308790667E-5 0.0 0.0 0.0 31 0.43851504008999465 2.4275633308790667E-5 0.0 0.0 0.0 32 0.45405144540762066 2.4275633308790667E-5 0.0 0.0 0.0 33 0.46672332599480937 2.4275633308790667E-5 0.0 0.0 0.0 34 0.47980789234824756 2.4275633308790667E-5 0.0 0.0 0.0 35 0.48954242130507264 2.4275633308790667E-5 0.0 0.0 0.0 36 0.498888540128957 2.4275633308790667E-5 0.0 0.0 0.0 37 0.5054672367556393 2.4275633308790667E-5 0.0 0.0 0.0 38 0.5110506324166612 2.4275633308790667E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATAA 15 2.0429563E-5 227.46712 44 GACGAAT 10 0.0018435293 227.46712 44 CTATCAT 1800 0.0 170.60034 44 GTCATAC 65 0.0 157.47723 44 GCTAATC 35 5.678885E-9 106.20469 43 GACGTCG 90 5.456968E-12 101.0965 44 GCGGATA 25 1.4121404E-5 99.12439 43 CTTACGA 130 0.0 96.236084 44 GCACTAT 60 4.683734E-7 94.77796 44 AAACTAC 530 0.0 91.175735 43 GTTTGAT 70 1.1722077E-6 81.23825 44 AATACGG 15 0.0013959878 78.72815 42 CGATATA 35 1.4661055E-9 78.72814 42 ACTTGTC 290 0.0 78.436935 44 CGTATGA 175 0.0 77.98872 44 GTACACT 90 5.885704E-8 75.82237 44 TCTAAGA 165 0.0 75.82237 44 AGATACG 230 0.0 74.17406 44 ATAACGA 70 0.0 73.104706 42 CCTATCA 2300 0.0 72.72713 43 >>END_MODULE