FastQCFastQC Report
Tue 24 May 2016
ERR840946.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840946.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8860614
Sequences flagged as poor quality0
Sequence length24-50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT231240.2609751423546946No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCAC224640.25352644861857204No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT153410.17313698576644915No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG121550.13718010964025742No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCA114730.12948312611293078No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA113730.1283545361529122No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG110280.12446090079084812No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT103040.11628990948031367No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG99230.11198998173264291No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98140.11075981867622266No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA93910.10598588314534409No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC93600.10563602025773834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA45400.0174.4454344
CTATCAT42750.0157.8808444
CACTGCA156000.0117.6252544
CGTACGA456.578599E-6115.59675644
TTAAGCC44900.089.2369143
CGACGGG55400.088.2625144
TTAATCG302.676158E-587.15034543
TCGATAG302.676158E-587.15034543
GTGTTAG19600.086.70569643
AGGTCTG35850.079.8052944
AAGTCGA1800.079.4727744
TCGCAAT751.8189894E-1278.4353143
GGCCATA500.00144627878.0278144
ACGCTTA851.6767135E-676.4978544
ATTAAGC49850.075.6597642
CGTATCC1550.075.61912542
ACGGGTA355.747525E-574.70029443
ACGTTAG1800.072.2479744
TCGCAAA13900.071.9460643
GCGATAC950.068.802943
Produced by FastQC (version 0.11.5)