##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840946.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8860614
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.76351932270156	38.0	35.0	39.0	30.0	39.0
2	36.62533589658685	39.0	35.0	39.0	33.0	39.0
3	36.73225139928226	39.0	35.0	39.0	33.0	39.0
4	37.16217296002286	39.0	35.0	39.0	33.0	41.0
5	37.80028031917427	40.0	35.0	41.0	34.0	41.0
6	37.80312628447645	39.0	35.0	41.0	34.0	41.0
7	37.54944251041745	39.0	35.0	41.0	33.0	41.0
8	37.58264145125835	39.0	35.0	41.0	33.0	41.0
9	37.4630384530914	39.0	35.0	41.0	33.0	41.0
10	37.02697928157123	38.0	35.0	40.0	33.0	41.0
11	37.0619459328665	38.0	35.0	40.0	33.0	41.0
12	37.10172726179021	38.0	35.0	40.0	33.0	41.0
13	36.95671947790525	38.0	35.0	40.0	33.0	41.0
14	36.939441555630346	38.0	35.0	40.0	33.0	41.0
15	36.94595272968668	38.0	35.0	40.0	33.0	41.0
16	36.80489343063584	38.0	35.0	40.0	33.0	41.0
17	36.78940308199861	38.0	35.0	40.0	33.0	41.0
18	36.806104069085954	38.0	35.0	40.0	33.0	41.0
19	36.81723930192648	37.0	35.0	40.0	33.0	41.0
20	36.81117730667423	37.0	35.0	40.0	33.0	41.0
21	36.693969853556425	37.0	35.0	40.0	33.0	41.0
22	36.49832528535833	36.0	35.0	40.0	33.0	41.0
23	36.15131321599158	35.0	35.0	40.0	32.0	41.0
24	36.06477101925442	35.0	35.0	40.0	32.0	41.0
25	35.99090233280984	35.0	35.0	39.0	32.0	41.0
26	35.872822542364155	35.0	35.0	39.0	32.0	41.0
27	35.72073585980696	35.0	34.0	39.0	32.0	41.0
28	35.53085404593809	35.0	34.0	39.0	31.0	41.0
29	35.42853518787036	35.0	34.0	39.0	31.0	41.0
30	35.34934354036929	35.0	34.0	39.0	31.0	41.0
31	35.23918114407219	35.0	34.0	38.0	31.0	41.0
32	35.182967302661986	35.0	34.0	38.0	31.0	41.0
33	35.0185029905096	35.0	34.0	37.0	31.0	40.0
34	35.003487717485775	35.0	34.0	37.0	31.0	40.0
35	34.89810422914405	35.0	34.0	37.0	31.0	40.0
36	34.84800748468468	35.0	34.0	37.0	31.0	40.0
37	34.756293598851016	35.0	34.0	36.0	31.0	40.0
38	34.7652879371028	35.0	34.0	36.0	31.0	40.0
39	34.71723681643076	35.0	34.0	36.0	31.0	40.0
40	34.55708229700175	35.0	34.0	36.0	31.0	39.0
41	34.42929305198995	35.0	34.0	35.0	31.0	39.0
42	34.397921851375365	35.0	34.0	35.0	31.0	39.0
43	34.28878260911614	35.0	34.0	35.0	31.0	39.0
44	34.142331830511935	35.0	33.0	35.0	30.0	38.0
45	33.9797054275742	35.0	33.0	35.0	30.0	38.0
46	33.77219221860559	35.0	33.0	35.0	29.0	37.0
47	33.69936733547978	35.0	33.0	35.0	29.0	37.0
48	33.67018053092757	35.0	33.0	35.0	29.0	37.0
49	33.78149174827562	35.0	33.0	35.0	30.0	37.0
50	33.65001674861525	35.0	33.0	35.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	35.0
21	192.0
22	500.0
23	1394.0
24	3427.0
25	7529.0
26	15073.0
27	30061.0
28	56387.0
29	101729.0
30	173145.0
31	288909.0
32	471946.0
33	828285.0
34	1575312.0
35	829934.0
36	1159480.0
37	1263909.0
38	1032005.0
39	660239.0
40	361117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.110443587769424	16.460913431055683	40.428642981174896
2	28.357662347101453	20.732163707842368	25.598316324354048	25.31185762070213
3	29.381033865147494	15.777732784658038	26.21896180106706	28.622271549127408
4	20.292092624732327	17.817929998982013	29.94530627335758	31.94467110292808
5	23.626703521900403	23.253873828608267	28.54337182502251	24.576050824468822
6	30.12529380018134	20.060889685522923	24.692306876250335	25.121509638045396
7	32.180230399383156	16.616049406959835	24.493359038098266	26.710361155558747
8	32.45421818397687	18.30818947761408	21.303162512214165	27.93442982619489
9	31.435135307778893	20.449530924154917	23.73353584751576	24.381797920550426
10	28.982494892566134	15.350764630983813	25.70932443282147	29.957416043628584
11	31.47912774442042	20.696274547113777	22.588344329185315	25.236253379280488
12	29.980755284001763	16.39571478906541	24.625257346725633	28.998272580207196
13	27.31338934299587	17.286849421496072	24.297684110830243	31.10207712467782
14	25.62168942242603	23.68251229542332	19.56307994005833	31.132718342092318
15	26.667316734483638	24.32813346795154	21.335417613271495	27.66913218429332
16	27.95863808083729	20.73474817885081	18.14891157655666	33.157702163755246
17	32.52022941073835	19.82373907722422	18.959306883247596	28.696724628789834
18	28.440308989873614	20.102861946136013	17.736344230772268	33.72048483321811
19	28.89866323033596	23.364667504983288	20.58770419295999	27.148965071720767
20	35.26762366580916	18.062472871518835	19.93443118050284	26.73547228216916
21	31.98339302445632	24.15588806825351	17.27147802624062	26.58924088104955
22	33.807803838424746	19.841220935704907	20.797114060041437	25.553861165828913
23	32.22924506134676	19.887368979170066	22.057861904378186	25.825524055104985
24	37.005584488840164	19.538702396921927	19.40147714368327	24.054235970554636
25	31.44462829544193	20.420536788296463	20.068301884045574	28.066533032216036
26	32.37541248934819	24.248563078700393	19.053467770069716	24.322556661881695
27	32.80544797472131	21.509360701866896	19.48308140747858	26.20210991593322
28	30.59651352308581	19.474848300405316	23.538008869081533	26.39062930742734
29	36.52898134190681	19.847296622069933	20.07015192855374	23.553570107469515
30	30.015611715098718	23.13899269466713	18.42369284946773	28.42170274076642
31	28.209247637288605	20.604017098425018	25.05029523889127	26.136440025395107
32	30.48704279621911	18.117681836017685	22.942645715670377	28.45262965209283
33	27.838747043106878	18.54913100657074	25.711303517278267	27.900818433044122
34	28.694770719840886	19.91920507885723	22.607440868920182	28.7785833323817
35	26.836411344977513	19.76763216480651	21.90084106462631	31.49511542558967
36	27.06930586026528	18.914134595031694	25.773597400456122	28.24296214424691
37	30.588260678812112	20.035487948754895	23.074073807691477	26.302177564741513
38	31.4708271802975	20.158007213068736	20.775246302496853	27.595919304136913
39	30.917844311919644	21.85504272962337	20.01015008615873	27.216962872298257
40	30.15229923011264	23.218306126117128	19.438559469736244	27.19083517403399
41	27.01661377818137	21.77267506966627	20.295586494474136	30.915124657678223
42	28.253542026162016	21.94478718950114	20.97580399047256	28.82586679386428
43	29.574333304188734	19.612499661831144	22.853170154935572	27.959996879044546
44	28.311659045505234	19.974994757677578	21.90031544930818	29.81303074750901
45	29.359414099024733	20.920028773752147	23.718256251433424	26.002300875789697
46	27.734164492929818	21.80220889071559	21.86765679400872	28.595969822345875
47	28.661523037020842	18.382694135742124	23.393601314447462	29.56218151278957
48	28.374803972943045	19.740083665416112	21.24850481735067	30.63660754429017
49	29.05107789693691	19.58671010493756	20.20725504962413	31.154956948501393
50	25.930594934740213	27.244763066910714	19.27339422651306	27.551247771836007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	62385.0
1	44731.5
2	27580.0
3	24864.0
4	18473.0
5	13592.5
6	10492.0
7	9784.0
8	11075.5
9	12221.0
10	12767.0
11	13058.5
12	13052.0
13	11976.0
14	10908.0
15	10443.0
16	10660.0
17	11404.0
18	12049.5
19	13362.5
20	15578.0
21	18055.5
22	19422.5
23	21173.5
24	24744.0
25	28165.5
26	30890.5
27	33051.0
28	34410.5
29	37223.5
30	42502.5
31	49259.0
32	56623.5
33	63751.5
34	76139.5
35	86790.5
36	91871.5
37	97608.0
38	106228.0
39	127769.5
40	159863.5
41	202229.0
42	244208.0
43	285548.0
44	307260.5
45	282150.0
46	265616.5
47	279537.0
48	323503.5
49	399761.5
50	480619.0
51	532161.0
52	561476.5
53	587155.5
54	604441.0
55	605416.0
56	610833.0
57	644080.0
58	677351.5
59	749662.0
60	828183.5
61	790277.5
62	700603.5
63	652070.0
64	622701.0
65	590649.0
66	543304.5
67	506784.5
68	478961.5
69	450722.5
70	430981.0
71	446250.5
72	465230.0
73	413731.5
74	329835.0
75	275967.5
76	233563.5
77	175099.5
78	134805.0
79	118080.5
80	100295.0
81	75847.0
82	53003.0
83	39948.0
84	34939.5
85	30857.5
86	25962.0
87	18683.0
88	9442.0
89	4342.5
90	2739.5
91	1705.0
92	1047.5
93	848.5
94	597.5
95	298.5
96	231.5
97	223.0
98	335.5
99	448.0
100	448.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	68470.0
25	49494.0
26	47896.0
27	57783.0
28	67285.0
29	62613.0
30	72378.0
31	65451.0
32	77179.0
33	60607.0
34	62169.0
35	48172.0
36	56971.0
37	55904.0
38	59541.0
39	59495.0
40	68348.0
41	68092.0
42	101263.0
43	178670.0
44	444205.0
45	773271.0
46	1063822.0
47	1025656.0
48	1494195.0
49	1334260.0
50	1337424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.018334040635533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5035864766253	11.613866673266891
2	12.429912149497246	3.982129698128018
3	4.2261815957126965	2.030891655495362
4	2.0015400087339987	1.28245345822391
5	1.222938854142848	0.9794721538466098
6	0.766442476083991	0.7366278962907106
7	0.5676661180838911	0.6365145852113037
8	0.4389235996007296	0.5624659869378117
9	0.36732850962442026	0.5295591692831492
>10	3.1992518756218606	11.486071602096727
>50	1.1977827674922419	13.939071269470485
>100	0.9828465601363232	27.28287860825716
>500	0.056718070118233425	6.130772908847057
>1k	0.03346967388602467	10.702182609521959
>5k	0.003807926969068675	4.340521343085828
>10k+	0.0016033376711868104	3.764520382036998
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	23124	0.2609751423546946	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAGCAC	22464	0.25352644861857204	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	15341	0.17313698576644915	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	12155	0.13718010964025742	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGACAGCA	11473	0.12948312611293078	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	11373	0.1283545361529122	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	11028	0.12446090079084812	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	10304	0.11628990948031367	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	9923	0.11198998173264291	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9814	0.11075981867622266	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	9391	0.10598588314534409	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	9360	0.10563602025773834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02154478233675454	0.0	0.0	0.0	0.0
2	0.08553583306980758	0.0	0.0	0.0	0.0
3	0.11448416554428395	0.0	0.0	0.0	0.0
4	0.13071328916935102	0.0	0.0	0.0	0.0
5	0.22363010057768007	0.0	0.0	0.0	0.0
6	0.25255586125295604	0.0	0.0	0.0	0.0
7	0.2725996189428859	0.0	0.0	0.0	0.0
8	0.29032976721477766	0.0	0.0	0.0	0.0
9	0.3012319462285571	0.0	0.0	0.0	0.0
10	0.31360129219036065	0.0	0.0	0.0	0.0
11	0.3331597561974825	0.0	0.0	0.0	0.0
12	0.3457209624524892	0.0	0.0	0.0	0.0
13	0.35768401602868605	0.0	0.0	0.0	0.0
14	0.3691956336208755	0.0	0.0	0.0	0.0
15	0.38763679356757896	0.0	0.0	0.0	0.0
16	0.4019360283610143	0.0	0.0	0.0	0.0
17	0.4237291004889729	0.0	0.0	0.0	0.0
18	0.4475197768461644	0.0	0.0	0.0	0.0
19	0.5021548168106634	0.0	0.0	0.0	0.0
20	0.5168152003913047	0.0	0.0	0.0	0.0
21	0.544296365917757	0.0	0.0	0.0	0.0
22	0.556755999076362	0.0	0.0	0.0	0.0
23	0.573222126593033	0.0	0.0	0.0	0.0
24	0.5870699254024608	0.0	0.0	0.0	0.0
25	0.6080165550604055	0.0	0.0	0.0	0.0
26	0.6695247078814177	0.0	0.0	0.0	0.0
27	0.6835982246828493	0.0	0.0	0.0	0.0
28	0.700109495797921	0.0	0.0	0.0	0.0
29	0.7142281561977534	0.0	0.0	0.0	0.0
30	0.7281323845051821	0.0	0.0	0.0	0.0
31	0.7401631534789801	0.0	0.0	0.0	0.0
32	0.7526905020351863	0.0	0.0	0.0	0.0
33	0.7643262645229778	0.0	0.0	0.0	0.0
34	0.7755783064243629	0.0	0.0	0.0	0.0
35	0.7865707726349438	0.0	0.0	0.0	0.0
36	0.7963782193875052	0.0	0.0	0.0	0.0
37	0.8038156272240276	0.0	0.0	0.0	0.0
38	0.8080704113732976	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	4540	0.0	174.44543	44
CTATCAT	4275	0.0	157.88084	44
CACTGCA	15600	0.0	117.62525	44
CGTACGA	45	6.578599E-6	115.596756	44
TTAAGCC	4490	0.0	89.23691	43
CGACGGG	5540	0.0	88.26251	44
TTAATCG	30	2.676158E-5	87.150345	43
TCGATAG	30	2.676158E-5	87.150345	43
GTGTTAG	1960	0.0	86.705696	43
AGGTCTG	3585	0.0	79.80529	44
AAGTCGA	180	0.0	79.47277	44
TCGCAAT	75	1.8189894E-12	78.43531	43
GGCCATA	50	0.001446278	78.02781	44
ACGCTTA	85	1.6767135E-6	76.49785	44
ATTAAGC	4985	0.0	75.65976	42
CGTATCC	155	0.0	75.619125	42
ACGGGTA	35	5.747525E-5	74.700294	43
ACGTTAG	180	0.0	72.24797	44
TCGCAAA	1390	0.0	71.94606	43
GCGATAC	95	0.0	68.8029	43
>>END_MODULE