FastQCFastQC Report
Tue 24 May 2016
ERR840945.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840945.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13482594
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA210770.15632748416217235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA208010.15428040034432544No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA189390.1404700015442132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA188880.14009173605613282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA174710.12958188906378104No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171360.1270972039950176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA158220.11735130494918114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT90850.0180.8263944
CCTATCA95100.075.70077543
AATCGCA18450.072.67097544
AACGACA13050.071.9969243
ACTTCTA30950.069.76932544
CCCTATC117350.064.8860242
TCGCAAA17950.063.78240243
AATACGG505.718175E-662.22250743
ACCACTC19650.061.0506244
TAATTCC22800.059.4251344
TACGCTA12950.058.79825242
TGTGTTA42000.058.62516442
ACATTGG15700.055.7347144
TGCACCG21000.054.51876443
ATCATAA16950.054.12244844
GTGTTAG33100.053.57526443
CTAATCG11950.051.5982242
ACCTTAG30350.051.267942
AACCTTA36850.051.05312341
TCGTCGA3350.050.55570644
Produced by FastQC (version 0.11.5)