##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840945.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13482594 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.33086414973261 39.0 37.0 39.0 34.0 39.0 2 37.81816370054605 39.0 38.0 39.0 35.0 39.0 3 37.97340719449091 39.0 38.0 39.0 35.0 40.0 4 38.559786640464 39.0 38.0 41.0 35.0 41.0 5 39.14870150358306 40.0 38.0 41.0 35.0 41.0 6 39.06595110703474 40.0 38.0 41.0 35.0 41.0 7 39.11164958315885 40.0 38.0 41.0 35.0 41.0 8 39.03417116913852 40.0 38.0 41.0 35.0 41.0 9 38.95253672994974 40.0 38.0 41.0 35.0 41.0 10 38.89724188090214 40.0 38.0 41.0 35.0 41.0 11 38.72816039702746 40.0 38.0 41.0 35.0 41.0 12 38.728243467095425 40.0 38.0 41.0 35.0 41.0 13 38.61358125891798 40.0 38.0 41.0 35.0 41.0 14 38.59432383708951 40.0 38.0 41.0 35.0 41.0 15 38.57607089555615 40.0 38.0 41.0 35.0 41.0 16 38.492942085180346 40.0 38.0 41.0 35.0 41.0 17 38.523578548757015 40.0 38.0 41.0 35.0 41.0 18 38.57959180555314 40.0 38.0 41.0 35.0 41.0 19 38.65873748033947 40.0 38.0 41.0 35.0 41.0 20 38.67593350359731 40.0 38.0 41.0 35.0 41.0 21 38.616109852451245 40.0 38.0 41.0 35.0 41.0 22 38.544595498462684 40.0 37.0 41.0 35.0 41.0 23 38.411629171656436 40.0 37.0 41.0 34.0 41.0 24 38.232886268028246 40.0 37.0 41.0 34.0 41.0 25 38.1588579400612 40.0 36.0 41.0 34.0 41.0 26 38.08200642540913 40.0 36.0 41.0 33.0 41.0 27 38.00440590893406 40.0 36.0 41.0 33.0 41.0 28 37.91365285030724 40.0 35.0 41.0 33.0 41.0 29 37.803335695865414 40.0 35.0 41.0 33.0 41.0 30 37.70173801668991 39.0 35.0 41.0 33.0 41.0 31 37.570685028578545 39.0 35.0 41.0 33.0 41.0 32 37.454751232374456 39.0 35.0 41.0 33.0 41.0 33 37.332117674085914 39.0 35.0 41.0 33.0 41.0 34 37.240351492919515 39.0 35.0 41.0 33.0 41.0 35 37.07735407508195 38.0 35.0 41.0 33.0 41.0 36 36.9954555660545 38.0 35.0 41.0 33.0 41.0 37 36.90075508049489 38.0 35.0 40.0 33.0 41.0 38 36.84789667846065 37.0 35.0 40.0 33.0 41.0 39 36.76160045872811 37.0 35.0 40.0 33.0 41.0 40 36.652862412658266 37.0 35.0 40.0 33.0 41.0 41 36.53734362741697 37.0 35.0 40.0 33.0 41.0 42 36.42823367371267 36.0 35.0 40.0 32.0 41.0 43 36.284257987745555 36.0 35.0 40.0 32.0 41.0 44 36.15118141122523 36.0 35.0 40.0 32.0 41.0 45 35.960941178709724 35.0 35.0 40.0 31.0 41.0 46 35.711990256200046 35.0 34.0 40.0 31.0 41.0 47 35.51201185056449 35.0 34.0 39.0 30.0 41.0 48 35.598466918276124 35.0 34.0 39.0 31.0 41.0 49 35.57200079540558 35.0 34.0 39.0 30.0 41.0 50 35.99925412457586 37.0 34.0 39.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 12.0 20 51.0 21 151.0 22 315.0 23 719.0 24 1562.0 25 3408.0 26 6696.0 27 13349.0 28 26556.0 29 51585.0 30 96443.0 31 171275.0 32 287279.0 33 491321.0 34 895603.0 35 810556.0 36 1313324.0 37 1983008.0 38 2202591.0 39 2589102.0 40 2537687.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 43.98021626995517 18.32150400731491 37.698279722729914 2 27.65139260293679 21.97677983925052 27.96662867694451 22.405198880868177 3 24.945170046654226 18.13591657510417 28.770168411212264 28.148744967029344 4 21.703397728953345 22.104233057822555 31.8699428314759 24.3224263817482 5 23.377393104027313 20.442134503197234 29.084662788184527 27.095809604590926 6 23.62906574209681 23.37433731224125 27.364860204201065 25.63173674146088 7 30.141291801859495 20.48191171520851 21.40221681376744 27.974579669164555 8 26.110479926933944 21.967234198404253 23.64992967970407 28.272356194957737 9 27.642840836118033 21.990122968918296 24.478561024681156 25.888475170282515 10 27.50701385801575 19.492769714789304 24.730767684616183 28.269448742578764 11 27.497075117740692 21.32289231582587 24.33708231516873 26.842950251264703 12 28.656102824130137 20.49121259603308 24.58325897820553 26.26942560163126 13 28.241865029830315 20.96233855295205 23.4880246338353 27.30777178338234 14 25.414649436154495 21.541492683084577 24.58331089699801 28.460546983762917 15 26.792151421306613 23.643588170050954 22.82287073244214 26.741389676200296 16 26.30341015979566 24.0543325713138 21.852775511893334 27.78948175699721 17 27.380213332834913 24.99826813742222 19.71648037462227 27.905038155120593 18 28.141906520362475 22.06953647050412 20.294470040409138 29.49408696872427 19 28.126553391728628 21.683883679950608 22.595978192327085 27.59358473599368 20 28.070503346759534 22.585045578024527 23.580276911104793 25.764174164111147 21 28.989829405231664 25.47936991946802 19.704650306906814 25.826150368393503 22 30.194968416315138 24.672826312206688 20.84362252545764 24.288582746020534 23 30.698558452475837 24.415909876096542 20.436141591150783 24.449390080276835 24 29.71167862801476 24.553242499180797 21.831785485790046 23.903293387014397 25 29.858527769692998 25.58422445457626 20.79832706705817 23.75892070867257 26 29.22893360784068 26.19091576406095 20.77518370156117 23.8049669265372 27 29.38034240574052 25.862568173941213 20.493752325046604 24.263337095271662 28 28.9085459671656 24.784987271636446 21.173312685429174 25.133154075768783 29 28.324711625345365 26.914571453555535 20.957761301417673 23.802955619681427 30 28.799195495765684 26.585185620337075 20.434971817616848 24.18064706628039 31 27.195759161488265 26.47358625737825 21.361195471039885 24.9694591100936 32 26.577567153289138 25.30349520110102 23.488375449428606 24.630562196181245 33 25.488750136908955 24.72715281174416 22.98579745066632 26.79829960068057 34 27.18591040308791 25.00747202381109 22.98097475009435 24.82564282300665 35 26.069655786598567 24.477186273674906 22.52572310731063 26.9274348324159 36 25.776030629260582 25.38528528305709 23.817682604029123 25.021001483653198 37 24.579386432228446 27.39340731695055 23.41745280341473 24.609753447406277 38 26.165882977674173 25.17650964365284 21.93664039419807 26.720966984474927 39 26.590506248164942 26.662083878988103 21.563714777114754 25.183695095732205 40 26.072150151908385 26.47419146165503 21.931312488757257 25.522345897679333 41 25.801242708394483 26.355860035864293 21.745303819120153 26.097593436621068 42 25.072675420559083 27.21522584107613 21.303759214940687 26.4083395234241 43 26.014945437359714 26.147605807920378 21.769251963994666 26.068196790725246 44 25.140577925643548 26.540387502847196 21.906425082953398 26.41260948855586 45 25.943556874745276 25.090162558879943 23.603264976263187 25.363015590111594 46 25.488173234671752 26.30042495445884 22.6918785460399 25.51952326482951 47 24.550946894334825 26.27860968720986 22.652489096427374 26.517954322027947 48 23.78812571065902 26.60082302998627 21.362327061044613 28.248724198310093 49 25.009588721018915 27.249625326381967 20.982650737826848 26.758135214772267 50 21.321666109704445 31.984594320242447 23.553268809522063 23.140470760531038 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315960.0 1 272124.5 2 182364.5 3 127740.5 4 109599.0 5 77959.0 6 44164.5 7 35030.5 8 39365.0 9 41660.0 10 41645.5 11 40840.0 12 38806.0 13 35253.0 14 33018.5 15 33116.5 16 35946.5 17 43355.5 18 55508.5 19 72248.5 20 90624.0 21 109042.5 22 121319.5 23 128975.0 24 136330.0 25 143420.5 26 149546.0 27 154746.0 28 160192.5 29 163951.0 30 170268.0 31 185216.5 32 206226.5 33 222323.0 34 246346.0 35 267805.5 36 276957.0 37 290593.5 38 299586.0 39 314619.5 40 340575.0 41 387183.0 42 444615.5 43 455984.0 44 442373.5 45 445426.0 46 474199.0 47 516036.5 48 570138.5 49 635986.5 50 713423.5 51 780941.0 52 815667.5 53 798412.5 54 767015.5 55 772702.5 56 797529.0 57 831515.5 58 849387.0 59 839799.5 60 826763.0 61 826246.0 62 823066.0 63 807269.5 64 771914.0 65 739999.0 66 705318.5 67 673874.5 68 652697.5 69 618079.0 70 552776.5 71 484591.5 72 447161.0 73 421719.0 74 388793.0 75 356598.5 76 319806.5 77 254106.5 78 201851.0 79 176704.0 80 149477.5 81 114391.5 82 83488.5 83 65171.5 84 54573.5 85 43521.5 86 30982.0 87 23810.5 88 16371.0 89 9734.0 90 5959.5 91 2892.5 92 1762.5 93 1602.0 94 1440.5 95 1282.5 96 1257.0 97 1150.0 98 603.0 99 56.0 100 56.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 100109.0 25 84966.0 26 80042.0 27 88138.0 28 92479.0 29 99700.0 30 95360.0 31 92834.0 32 103701.0 33 88426.0 34 86145.0 35 71570.0 36 66664.0 37 84404.0 38 82244.0 39 80238.0 40 93702.0 41 96516.0 42 143542.0 43 218531.0 44 604689.0 45 1241203.0 46 1484575.0 47 1739625.0 48 2153440.0 49 2400585.0 50 1909166.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.679049166527193 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99886767757297 12.728715216060982 2 12.530851930594784 4.430670947589147 3 4.302957377204149 2.2821658509918916 4 2.15571177814045 1.524437380584271 5 1.290624722729124 1.1408508964331856 6 0.8662763992562619 0.9188965832552156 7 0.6592021547749386 0.8157847113462766 8 0.4887060363307825 0.6911886435416422 9 0.36862032957177043 0.5865171237252708 >10 3.1589421551198655 12.8191668647924 >50 1.2946541990208051 16.507203032394038 >100 0.8071341261278326 24.13535363010019 >500 0.04561298801714345 5.512359516544449 >1k 0.028459385685910013 9.620444314770076 >5k 0.002079224524997992 2.4280508127780065 >10k+ 0.001299515328123745 3.8581944750930806 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAA 21077 0.15632748416217235 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 20801 0.15428040034432544 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 18939 0.1404700015442132 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 18888 0.14009173605613282 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17471 0.12958188906378104 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17136 0.1270972039950176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 15822 0.11735130494918114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03492651339942447 0.0 0.0 0.0 0.0 2 0.07813036571449085 0.0 0.0 0.0 0.0 3 0.09937998578018443 0.0 0.0 0.0 0.0 4 0.12395982553505654 0.0 0.0 0.0 0.0 5 0.1431623617829032 0.0 0.0 0.0 0.0 6 0.17451389547145008 0.0 0.0 0.0 0.0 7 0.19181768730854018 0.0 0.0 0.0 0.0 8 0.2133269013366419 0.0 0.0 0.0 0.0 9 0.23665327310160048 0.0 0.0 0.0 0.0 10 0.25556654750562097 0.0 0.0 0.0 0.0 11 0.276608492401388 0.0 0.0 0.0 0.0 12 0.29553660074611754 0.0 0.0 0.0 0.0 13 0.3147762218457368 0.0 0.0 0.0 0.0 14 0.331078722684967 0.0 0.0 0.0 0.0 15 0.3494134734013351 0.0 0.0 0.0 0.0 16 0.3642770819917888 0.0 0.0 0.0 0.0 17 0.38307910184049154 0.0 0.0 0.0 0.0 18 0.40530034502262696 0.0 0.0 0.0 0.0 19 0.42252255018581736 0.0 0.0 0.0 0.0 20 0.4404567845030415 0.0 0.0 0.0 0.0 21 0.457723491488359 0.0 0.0 0.0 0.0 22 0.474997615444031 0.0 0.0 0.0 0.0 23 0.4932062776643723 0.0 0.0 0.0 0.0 24 0.5119192938688208 0.0 0.0 0.0 0.0 25 0.531544597426875 0.0 0.0 0.0 0.0 26 0.5557090868418941 0.0 0.0 0.0 0.0 27 0.5738880811808172 0.0 0.0 0.0 0.0 28 0.5939954878119151 0.0 0.0 0.0 0.0 29 0.6148149235970467 0.0 0.0 0.0 0.0 30 0.635345097538352 0.0 0.0 0.0 0.0 31 0.6570694037067347 0.0 0.0 0.0 0.0 32 0.6778665885808027 0.0 0.0 0.0 0.0 33 0.6990049540911786 0.0 0.0 0.0 7.416970354517832E-6 34 0.7172061993411654 0.0 0.0 0.0 7.416970354517832E-6 35 0.7325148261528902 0.0 0.0 0.0 7.416970354517832E-6 36 0.7457021994432229 0.0 0.0 0.0 7.416970354517832E-6 37 0.7543355529358816 0.0 0.0 0.0 7.416970354517832E-6 38 0.7606399777372218 0.0 0.0 0.0 7.416970354517832E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCAT 9085 0.0 180.82639 44 CCTATCA 9510 0.0 75.700775 43 AATCGCA 1845 0.0 72.670975 44 AACGACA 1305 0.0 71.99692 43 ACTTCTA 3095 0.0 69.769325 44 CCCTATC 11735 0.0 64.88602 42 TCGCAAA 1795 0.0 63.782402 43 AATACGG 50 5.718175E-6 62.222507 43 ACCACTC 1965 0.0 61.05062 44 TAATTCC 2280 0.0 59.42513 44 TACGCTA 1295 0.0 58.798252 42 TGTGTTA 4200 0.0 58.625164 42 ACATTGG 1570 0.0 55.73471 44 TGCACCG 2100 0.0 54.518764 43 ATCATAA 1695 0.0 54.122448 44 GTGTTAG 3310 0.0 53.575264 43 CTAATCG 1195 0.0 51.59822 42 ACCTTAG 3035 0.0 51.2679 42 AACCTTA 3685 0.0 51.053123 41 TCGTCGA 335 0.0 50.555706 44 >>END_MODULE