FastQCFastQC Report
Tue 24 May 2016
ERR840944.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840944.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390896
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCGTGGAGAAAGAAATGGCTCC28550.7303732962220131No Hit
AATTCGTGGAGAAAGAAATGGCTC20850.5333899553845524No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC15210.3891060537841267No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC15010.3839896033727641No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA12240.3131267651753919No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT10710.27398591952846796No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG10610.27142769432278663No Hit
CCCGGCGAGCTGGCAACATGCTTTGACCCGGTAATGTCCGAATGGGGAAA9090.23254267119643077No Hit
ACCAGGCCCCCGGCTTGGGGCGCCTTCCTTCCCCATGGCGGG8510.2177049650034792No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT7570.1936576480700749No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC7110.1818898121239409No Hit
CCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTT7100.18163398960337276No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG7020.17958740943882773No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7000.17907576439769146No Hit
AGCGGCTCTGACAGGACGGGTCGCAGGGGGTCGCCTGGCCGGAGCTGG6570.16807539601326185No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA6520.1667962834104212No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG6460.1652613482870124No Hit
AAGCAAGCGCCGTGCGCAGCCCAGGTTCCCGCCCACGCCTCTTCCTCCAT6410.16398223568417175No Hit
CTCTTGCCTGCCACGATGTAAGATGTTCCTTTGCTCTCCCTTTGTCTTC6250.15988907535508165No Hit
ACGCAAGCGCGGCGCGCAGCCCCGGTTCCCGCCAGCGCCTCTCCCTCCA6110.15630756006712782No Hit
CCTGCGCCTCTCCCTCCACACCTCCCTGCAAGCTAAGGAAGCCGGCTCC6080.15554009250542344No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG5940.1519585772174696No Hit
AGCACCTGGCGCCGCCTGCCTGACGTCACGGTCACTGACAGCGTGAGC5890.15067946461462894No Hit
AGGCGGTGCGACAGCAGCTGGAGGGCAGAGGAGGCGGCGCGGGG5730.14658630428553887No Hit
CTCTCCGCCAGACCGCCGCCGCGCCGCCATCATGGACACCAGCCGTGTG5480.1401907412713356No Hit
CTCTTCGTGCACCCTCCCTTCTCTCTTGAGCTCTTGCCTTTGCCTTCT5470.13993491875076747No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG5440.13916745118906307No Hit
CCCGGCGAGCTGGCAACATGCTTTGACCCGGTAATGTCCGAATGGGGA5400.13814416110679054No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC5390.1378883385862224No Hit
AGAGGCCGCGTTCAGGCCAGTGCAGAGCGCCACATGGCGCTACCAAGTC5360.13712087102451803No Hit
CTCTAGCCCAGCCACAACCATCAGAAAGCACGCAGTGCACCTGCACCCC5250.1343068232982686No Hit
AACCCCATTATCCATCAAAAACCATCAAGGTGGCGGCCCTCACTGAGGG5170.13226024313372353No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG5170.13226024313372353No Hit
CTGCTGTCTGGCCGTTAGAGCCAGCCAGTCACCTAGCTGCTTTTATAC5170.13226024313372353No Hit
AAATAATTTGGGAACCATTGCCAAGTCTGGTACTAAAGCATTCATGGAGG5130.13123695305145103No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT5070.12970201792804223No Hit
AGTCTTCTCGAGCACATCGTCGCAAACGGGGCCGGAAAGCGTGGCAGCGC5060.1294461954074741No Hit
ATTGAGAAGAGGAACACAGCCACTCCCAGAAGCACAAGAGAACCCAACC5010.12816708280463346No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG4950.12663214768122466No Hit
AGAGTTGGGGAGTTTTAAGAGGTTTAGAAGCCTGGCTGTCAATACCCAC4910.12560885759895216No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC4810.12305063239327085No Hit
CCCTCTCGGGACGCCCCGCCCCTCCTCCCATCCTCCCTGCCCGCGCGC4780.12228316483156645No Hit
ACCATCATCGGACCTGCCACTGTTGGAGGCATCAAGCCTGGGTGCTTTA4770.12202734231099832No Hit
AGCTAGGGACAAACTGGCGATTCTGAATAGGCCTGGAACCCTTAGCTCT4640.11870164954361263No Hit
ACCTGGTTGATCCTGCCAGTAGGATATGC4630.1184458270230445No Hit
CTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGA4570.11691089189963572No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT4530.1158876018173632No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC4440.11358519913225No Hit
ACGACAGTTGCTGCAGGGAATCTTTTAAACGAGAGCGAGAAGGACTGCG4440.11358519913225No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA4410.11281773157054562No Hit
CCGCGGTGCGGAGAGCAGGTGGTGCTGGAAGCGCGTGAGGCCGGGAGC4350.11128279644713683No Hit
CTCCTGGGCGGGGCAAGTGAGGCCAAGTACTGGGCCTCCAGGGCGTCGTA4340.1110269739265687No Hit
AAGTTATGGACCGCTAGCATCTGTCACTGGCCACCGGTTTCCGGGAG4260.10898039376202365No Hit
TCTTGTTGGCGTTGTACTTGGTCTTGCCCCACACCTGCCCGCTTCTCCC4260.10898039376202365No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC4240.1084687487208874No Hit
AAGCGAGGCAACTTCCCACCAGGGCTACTGGTCATTGCCCCTGGCAACCG4230.10821292620031926No Hit
CGCTACCCTCCCGCCGCCCGCGGTCCTCCGTCGGTTCTCTCGTTAGTC4210.107701281159183No Hit
CTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGCAC4210.107701281159183No Hit
CTGCAGCGGGGCGGTGACGTGTAGGCCGGCGCCAGCTCCACTCTGTCCC4210.107701281159183No Hit
CTCTTGCCTGTTACCATATAAGATGTGACTTTGCTCCTCAGTCACCTTC4190.10718963611804674No Hit
ACTGCCGCCCTTGTGCCCTGCGGACACAACCTGTTCTACATGGAGTGTG4170.10667799107691048No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA4150.10616634603577421No Hit
AGCCTCATAGTCTCAGGGGGAGGAGGGAGCAGGAGCACCTGAAGGCTCC4130.10565470099463796No Hit
AACGTGCCAGTAAGTAAGATGGCGAGAAAAGCAAGAGAGAGTGGGACCG4130.10565470099463796No Hit
AGGCGGTGCGACAGCAGCTGGAGGGCAGAGGAGGCGGCGCGGGGTGTCCT4120.10539887847406983No Hit
CCCTGGGGCCGCCTCCCCGCGGGTTCCGTTGGCTGTGGCGG4100.10488723343293356No Hit
AGAGGAGGCTGCAACGCCGAGCGGAGGAGGCAGGAACCGGAGCGCGAG4090.10463141091236544No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4090.10463141091236544No Hit
CTTTTCCAGCCCCGGTACCGGACCCTGCAGCCGCAGAGATGTTGATGCC4080.10437558839179731No Hit
CTCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGG4080.10437558839179731No Hit
AGGTATTGGAAAAACCATTTCATAACTTTGTCAAAGTTAAATTATAGGCT4080.10437558839179731No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATT4070.10411976587122918No Hit
CCTTTTCCGGTTGCGGCGCCGCGCGGTGAGGTTGTCTAGTCCACGCTCG3980.101817363186116No Hit
TGCAAACTGATCCATCCTGTTTTTCCTTTTGTTACAACACAGTAGTGTT3960.10130571814497974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCC201.50485E-8390.6203644
TTATCCT103.6370932E-4390.6203644
GGCCATA103.6370932E-4390.6203644
GTTGTGC201.50485E-8390.6203644
CACTACG103.6370932E-4390.6203644
CAAATAC103.6370932E-4390.6203644
ATAACGT103.6370932E-4390.6203644
GTGCCTG201.50485E-8390.6203644
TGATTGA259.640644E-11390.6203344
ACGGAGT600.0390.6203344
CCCCCAT259.640644E-11390.6203344
TCCCGCG152.3424018E-6390.6203344
CACGTAT259.640644E-11390.6203344
CGCGTAT152.3424018E-6390.6203344
ATACCTC259.640644E-11390.6203344
GCGAATG550.0355.109444
TAACAGA500.0351.558344
CACCATA450.0347.2180844
CTGGCAC550.0319.5984844
CGCATGA254.58258E-8312.4962544
Produced by FastQC (version 0.11.5)