FastQCFastQC Report
Tue 24 May 2016
ERR840943.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840943.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768037
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT143351.8664465383829165No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC82911.0795052842506285No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA50300.6549163647063878No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATGAGCACTG36510.47536772316958686No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATGAGCAC16020.20858370104565277No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATGAGC15450.20116218359271754No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC14330.18657955280800273No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA13020.16952308287230955No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG12600.16405459632804148No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG12370.16105994893475184No Hit
TCTCTAGGGTGATACGTGGGTGAGAAAGGTCCTGGTCCGCGCCAGAGC12230.1592371200866625No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12040.1567632809356841No Hit
TCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCCTCTGGG10990.14309206457501394No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT10650.1386651945153684No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA10630.13840479039421277No Hit
CACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGG9470.12330135136718674No Hit
TCCGCGGTTTCGCCGCGTGACTTGCTTTTTGCGGCTGGGG9410.12252013900371987No Hit
CGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCAGCGGAGG9270.12069731015563054No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC8800.11457781330847343No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT8530.11106235767287254No Hit
AGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCACGGA8480.11041134736998347No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC8450.11002074118825005No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA8450.11002074118825005No Hit
CTGCTCTCTTCTTCAGCCATTAGTGCCGGGCAGCCCACTAGCTGCTGT8240.10728649791611601No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCG8030.10455225464398199No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA7880.10259922373531483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTT400.0267.1625744
CATTGTC201.0100666E-7267.1625744
ACTAGTG259.567884E-10267.1625744
GTGTACG201.0100666E-7267.1625744
GTCCATT100.0011376953267.1625744
GGTCTAT201.0100666E-7267.1625744
AATCGCA1200.0244.8990344
GGATACC400.0233.7672644
CTCAACT352.7284841E-11228.9964844
CTTATCA700.0228.9964844
CTATCAT19600.0226.9518744
GTTAGTA253.0729097E-7213.7300444
TAGCAGG850.0204.3007844
GTATATC203.380744E-5200.3719344
GTGCGTT203.380744E-5200.3719344
ACCACTC950.0196.8566444
AGGGTAC357.1522663E-9190.830444
AAGAATC501.8189894E-12187.013844
CTATAAT150.0038289765178.1083844
CCCATAC1950.0178.1083844
Produced by FastQC (version 0.11.5)