##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840943.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768037
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.20420500574842	39.0	37.0	39.0	34.0	39.0
2	37.689770154302465	39.0	38.0	39.0	35.0	39.0
3	37.97944369867597	39.0	38.0	39.0	35.0	40.0
4	38.39847038619233	39.0	38.0	41.0	35.0	41.0
5	39.10513686189598	40.0	38.0	41.0	35.0	41.0
6	39.08074741190854	40.0	38.0	41.0	35.0	41.0
7	38.955033416358845	40.0	38.0	41.0	35.0	41.0
8	38.99384534859649	40.0	38.0	41.0	35.0	41.0
9	38.88168538755294	40.0	38.0	41.0	35.0	41.0
10	38.493853811730425	40.0	37.0	41.0	34.0	41.0
11	38.47243947882719	40.0	37.0	41.0	34.0	41.0
12	38.442522951368225	40.0	37.0	41.0	34.0	41.0
13	38.25563351765605	40.0	37.0	41.0	34.0	41.0
14	38.33475210178676	40.0	37.0	41.0	34.0	41.0
15	38.252452681316136	40.0	37.0	41.0	34.0	41.0
16	38.19839669182605	40.0	36.0	41.0	34.0	41.0
17	38.14158562673413	40.0	36.0	41.0	34.0	41.0
18	38.18866148375664	40.0	36.0	41.0	34.0	41.0
19	38.22777678679543	40.0	36.0	41.0	34.0	41.0
20	38.10350412805633	40.0	35.0	41.0	34.0	41.0
21	38.01750176098287	40.0	35.0	41.0	34.0	41.0
22	37.87405294276187	39.0	35.0	41.0	34.0	41.0
23	37.593944041758405	39.0	35.0	41.0	33.0	41.0
24	37.40017993924772	39.0	35.0	41.0	33.0	41.0
25	37.4208851972926	39.0	35.0	41.0	33.0	41.0
26	37.30306366625884	39.0	35.0	41.0	33.0	41.0
27	37.25493168260519	38.0	35.0	41.0	33.0	41.0
28	37.1127816052863	38.0	35.0	41.0	33.0	41.0
29	36.87815685425338	37.0	35.0	40.0	33.0	41.0
30	36.828808587319784	37.0	35.0	40.0	33.0	41.0
31	36.64496536940653	37.0	35.0	40.0	33.0	41.0
32	36.51305961753247	37.0	35.0	40.0	33.0	41.0
33	36.34520387573944	36.0	35.0	40.0	32.0	41.0
34	36.27439415345244	36.0	35.0	40.0	32.0	41.0
35	36.182234731858465	35.0	35.0	40.0	32.0	41.0
36	36.09775175604484	35.0	35.0	40.0	32.0	41.0
37	36.00244938850188	35.0	35.0	39.0	32.0	41.0
38	35.93090493233053	35.0	35.0	39.0	32.0	41.0
39	35.89071078220463	35.0	35.0	39.0	32.0	41.0
40	35.779329172715755	35.0	35.0	39.0	32.0	41.0
41	35.5647478172057	35.0	35.0	39.0	31.0	41.0
42	35.358932189602896	35.0	34.0	39.0	31.0	41.0
43	35.37460311261839	35.0	34.0	39.0	31.0	40.0
44	35.21579384999153	35.0	34.0	38.0	31.0	40.0
45	35.09203216326208	35.0	34.0	38.0	31.0	40.0
46	34.797190134709226	35.0	34.0	37.0	30.0	40.0
47	34.711900928681956	35.0	34.0	37.0	30.0	40.0
48	34.66049669824064	35.0	34.0	37.0	30.0	39.0
49	34.748893815002056	35.0	34.0	37.0	30.0	39.0
50	35.140664602755585	36.0	34.0	37.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	4.0
22	14.0
23	36.0
24	93.0
25	224.0
26	535.0
27	1104.0
28	2206.0
29	4326.0
30	7513.0
31	13032.0
32	20822.0
33	36335.0
34	71600.0
35	62627.0
36	94250.0
37	120027.0
38	125254.0
39	119848.0
40	88185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.39953934510967	14.7621794262516	37.83828122863873
2	24.90986762356501	16.348561332331645	36.55136406188764	22.190206982215702
3	34.46044917106858	14.742128308922616	24.692039576218335	26.105382943790467
4	22.473136059851285	20.925033559581113	29.571882604614103	27.0299477759535
5	25.46830426138324	21.403005323962258	25.2508668202183	27.877823594436208
6	27.119787197752192	25.20959276701513	21.688798846930553	25.981821188302128
7	25.80852224567306	20.948860536666853	21.849077583501835	31.39353963415825
8	29.778122668569353	21.82681303114303	19.509216352858	28.885847947429617
9	30.539674520888966	21.547008802961315	25.810345074521152	22.102971601628568
10	33.23589879133427	18.503145030773258	25.061162417956428	23.199793759936043
11	29.924990592901125	20.220249805673422	21.475788275825252	28.3789713256002
12	25.94666663194612	23.277784794222153	24.634360063382363	26.141188510449364
13	29.091437001081978	20.586117595897072	21.82928687029401	28.493158532726937
14	23.893249934573465	24.38189826792199	19.706993282875697	32.017858514628855
15	25.84562983293774	26.540257826120357	20.418156937751696	27.195955403190208
16	26.602754815197706	23.057613109785073	17.29096384679384	33.048668228223384
17	28.960323526080124	25.423514752544474	18.03611023948065	27.580051481894753
18	24.9580423859788	25.66647179758267	17.607745460179654	31.767740356258876
19	29.352752536661647	23.844814768038518	18.49884836277419	28.303584332525645
20	29.512901071172355	20.8259497914814	23.605893986878236	26.05525515046801
21	27.408054559871463	28.098516087115595	18.558611108579402	25.93481824443354
22	34.07101480788035	23.506680016717947	19.84526787120933	22.577037304192377
23	30.98613738661028	21.346497629671486	22.766090696151355	24.901274287566874
24	31.57907757048163	22.106747461385325	25.341747858501606	20.972427109631436
25	27.013133630896252	23.64826281930387	19.970636617002995	29.36796693279688
26	27.1082377762179	26.742910423682275	23.749306944775515	22.39954485532431
27	29.124108831660116	28.10774704707485	17.89254394666878	24.875600174596247
28	28.82051922448448	22.527492965679958	20.665756212653008	27.986231597182552
29	27.560570699901355	27.293238911745753	21.11413196031727	24.03205842803562
30	29.259830118777103	26.85606859750567	18.582656849873754	25.30144443384348
31	30.59955991133269	21.721190567053977	22.786632780380597	24.892616741232736
32	25.550156759049276	26.323655956502947	21.075807557981758	27.05037972646603
33	24.20216099331318	22.23605649709733	22.357948089332076	31.20383442025742
34	29.120186743125505	22.902401181300153	23.608516685587084	24.368895389987262
35	24.298430329622388	21.96336037540095	20.576860155876407	33.16134913910025
36	24.892639245350985	22.628073078481698	26.715942861002297	25.763344815165024
37	26.279349150355095	27.9717053885132	19.905420492509627	25.843524968622074
38	27.93169107457955	22.46344509750585	18.2820798019833	31.3227840259313
39	28.73045922611365	23.752258841752	21.947336718010497	25.569945214123855
40	26.98737933148631	22.62133367266081	22.940465379921456	27.450821615931424
41	26.666013084328217	26.458525079271244	19.777206462270986	27.098255374129554
42	23.76469718257783	23.994379945278414	20.010352732381868	32.23057013976189
43	25.129692432930913	22.241463729675008	21.126424213659835	31.50241962373424
44	23.606239317555396	27.414193985494972	18.057989313705868	30.921577383243772
45	28.61228541936502	21.440640635375644	26.120816047442787	23.826257897816554
46	25.29062966844916	28.03860203030911	21.028859420159044	25.641908881082685
47	24.91766014125612	26.23889388820536	22.945522832982387	25.897923137556127
48	24.486772074162168	23.511701680262693	19.120682020102993	32.88084422547215
49	23.692073144018718	28.35382148583645	21.04467673112476	26.90942863902007
50	23.876761705971603	25.287880515334628	27.685642370398956	23.149715408294817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6700.0
1	5045.5
2	3676.5
3	3643.0
4	2858.5
5	2175.0
6	1887.5
7	1975.5
8	2727.0
9	3679.5
10	4241.0
11	4314.0
12	3901.5
13	3059.5
14	2310.0
15	2056.0
16	1980.0
17	2023.5
18	2611.0
19	3780.5
20	4898.0
21	5448.0
22	5306.5
23	4848.0
24	4366.0
25	4588.5
26	5078.5
27	5183.5
28	5464.0
29	6168.0
30	7052.0
31	8205.5
32	9976.5
33	10844.0
34	10739.0
35	10910.0
36	10591.0
37	9233.5
38	9690.5
39	12937.5
40	16014.5
41	19572.5
42	22914.0
43	24384.5
44	24253.5
45	24406.0
46	25732.5
47	28108.0
48	34396.5
49	46569.0
50	55840.5
51	59421.0
52	62401.5
53	59135.0
54	54064.0
55	53900.0
56	56288.0
57	54768.0
58	49393.5
59	46374.0
60	49379.5
61	53261.0
62	50994.5
63	46693.0
64	43056.5
65	40514.5
66	40584.5
67	43040.5
68	43913.0
69	40471.5
70	34561.5
71	30180.5
72	24458.5
73	18295.0
74	16570.0
75	16074.0
76	14709.5
77	12243.5
78	9856.5
79	8622.5
80	7344.0
81	5491.0
82	4413.5
83	3908.5
84	3226.0
85	2492.0
86	1838.0
87	1537.5
88	923.0
89	419.5
90	235.5
91	132.5
92	83.0
93	34.5
94	22.5
95	9.0
96	7.5
97	7.0
98	5.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	4501.0
25	4217.0
26	3289.0
27	3292.0
28	4604.0
29	3967.0
30	5225.0
31	5651.0
32	7236.0
33	5493.0
34	5380.0
35	4494.0
36	3981.0
37	4544.0
38	4903.0
39	5696.0
40	8151.0
41	7263.0
42	10344.0
43	16376.0
44	41034.0
45	65523.0
46	95034.0
47	88788.0
48	130789.0
49	114239.0
50	114023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.16263997018156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.20814151493386	8.319860099453608
2	14.806293899449921	3.897798317823099
3	6.600405675364776	2.606362906859088
4	3.4476276224242266	1.8151952458089256
5	2.145480517974976	1.4120093810571626
6	1.3020760539169176	1.028325498690186
7	0.8723808818380845	0.8037984825152946
8	0.6415446994047072	0.675553752243401
9	0.45068015468258904	0.5338926556013389
>10	3.4355617998892205	9.598624425050096
>50	1.163174501183865	11.28303668112548
>100	1.850686577484981	46.911920442834756
>500	0.06095673932320942	5.1499135609995905
>1k	0.011991489702926444	2.326030480747753
>5k	0.001998581617154407	1.7521517775413264
>10k+	9.992908085772036E-4	1.8855262916488937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	14335	1.8664465383829165	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8291	1.0795052842506285	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5030	0.6549163647063878	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATGAGCACTG	3651	0.47536772316958686	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATGAGCAC	1602	0.20858370104565277	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATGAGC	1545	0.20116218359271754	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	1433	0.18657955280800273	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	1302	0.16952308287230955	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	1260	0.16405459632804148	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	1237	0.16105994893475184	No Hit
TCTCTAGGGTGATACGTGGGTGAGAAAGGTCCTGGTCCGCGCCAGAGC	1223	0.1592371200866625	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1204	0.1567632809356841	No Hit
TCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCCTCTGGG	1099	0.14309206457501394	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	1065	0.1386651945153684	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	1063	0.13840479039421277	No Hit
CACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGG	947	0.12330135136718674	No Hit
TCCGCGGTTTCGCCGCGTGACTTGCTTTTTGCGGCTGGGG	941	0.12252013900371987	No Hit
CGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCAGCGGAGG	927	0.12069731015563054	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	880	0.11457781330847343	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	853	0.11106235767287254	No Hit
AGCCATTGTGGCTCAGGCCGGTTGCGCCTGCCCTCGGGCCCTCACGGA	848	0.11041134736998347	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	845	0.11002074118825005	No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA	845	0.11002074118825005	No Hit
CTGCTCTCTTCTTCAGCCATTAGTGCCGGGCAGCCCACTAGCTGCTGT	824	0.10728649791611601	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCG	803	0.10455225464398199	No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATA	788	0.10259922373531483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.09674013100931335	0.0	0.0	0.0	0.0
2	0.126556402881632	0.0	0.0	0.0	0.0
3	0.15025317790679354	0.0	0.0	0.0	0.0
4	0.22707239364770188	0.0	0.0	0.0	0.0
5	0.28618412915002794	0.0	0.0	0.0	0.0
6	0.32941121326186107	0.0	0.0	0.0	0.0
7	0.4813570179561662	0.0	0.0	0.0	0.0
8	0.5200270299477759	0.0	0.0	0.0	0.0
9	0.5769253304202793	0.0	0.0	0.0	0.0
10	0.5941120024165503	0.0	0.0	0.0	0.0
11	0.6109080682310878	0.0	0.0	0.0	0.0
12	0.6942373870008867	0.0	0.0	0.0	0.0
13	0.7184549702683595	0.0	0.0	0.0	0.0
14	0.7409799267483207	0.0	0.0	0.0	0.0
15	0.7595988214109477	0.0	0.0	0.0	0.0
16	0.7776969078312633	0.0	0.0	0.0	0.0
17	0.8067319673401151	0.0	0.0	0.0	0.0
18	0.8636302678126184	0.0	0.0	0.0	0.0
19	0.9248252362841894	0.0	0.0	0.0	0.0
20	0.9421421103410382	0.0	0.0	0.0	0.0
21	0.9703959574864232	0.0	0.0	0.0	0.0
22	0.9812027285143815	0.0	0.0	0.0	0.0
23	0.9966967737231409	0.0	0.0	0.0	0.0
24	1.0088055653568773	0.0	0.0	0.0	0.0
25	1.023388196141592	0.0	0.0	0.0	0.0
26	1.0634904307995579	0.0	0.0	0.0	0.0
27	1.078463667766006	0.0	0.0	0.0	0.0
28	1.0974731686103665	0.0	0.0	0.0	0.0
29	1.1125766076373924	0.0	0.0	0.0	0.0
30	1.1249458033922846	0.0	0.0	0.0	0.0
31	1.1354921702990872	0.0	0.0	0.0	0.0
32	1.1503352052049576	0.0	0.0	0.0	0.0
33	1.1620533906569606	0.0	0.0	0.0	0.0
34	1.1745527884724303	0.0	0.0	0.0	0.0
35	1.1832763265311437	0.0	0.0	0.0	0.0
36	1.1916092584081235	0.0	0.0	0.0	0.0
37	1.198119361437014	0.0	0.0	0.0	0.0
38	1.2018952211937706	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTT	40	0.0	267.16257	44
CATTGTC	20	1.0100666E-7	267.16257	44
ACTAGTG	25	9.567884E-10	267.16257	44
GTGTACG	20	1.0100666E-7	267.16257	44
GTCCATT	10	0.0011376953	267.16257	44
GGTCTAT	20	1.0100666E-7	267.16257	44
AATCGCA	120	0.0	244.89903	44
GGATACC	40	0.0	233.76726	44
CTCAACT	35	2.7284841E-11	228.99648	44
CTTATCA	70	0.0	228.99648	44
CTATCAT	1960	0.0	226.95187	44
GTTAGTA	25	3.0729097E-7	213.73004	44
TAGCAGG	85	0.0	204.30078	44
GTATATC	20	3.380744E-5	200.37193	44
GTGCGTT	20	3.380744E-5	200.37193	44
ACCACTC	95	0.0	196.85664	44
AGGGTAC	35	7.1522663E-9	190.8304	44
AAGAATC	50	1.8189894E-12	187.0138	44
CTATAAT	15	0.0038289765	178.10838	44
CCCATAC	195	0.0	178.10838	44
>>END_MODULE