FastQCFastQC Report
Tue 24 May 2016
ERR840942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356777
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT46250.19624258043930334No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC43040.18262228458611063No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG39170.16620155407151377No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35840.15207208827988392No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT33290.14125222708809532No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC27600.11710908584053561No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG27570.11698179335592634No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA27220.11549671436881809No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG26350.11180523231514904No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT26330.11172037065874285No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT26130.110871754094681No Hit
TTCCCACGGGGAGGCAGGAAGAAATAAAGG23970.101706695202813No Hit
AATTCGTGGAGAAAGAAATGGCTCC23840.1011550944361728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC700.0401.9558744
AAAACGT258.367351E-11401.9558744
TATGATT152.102921E-6401.9558744
TATCTAG103.3490438E-4401.9558744
ATCGACT152.102921E-6401.9558744
ATCTAGT206.6330704E-6301.4669244
CACGAGT206.6330704E-6301.4669244
GACGAAC600.0301.466944
GTGTTAC750.0267.9705844
CCCAGTA309.971518E-8267.9705844
GCGTAAA700.0258.400244
ATAAATG3800.0243.2890844
ATATCTT352.1507913E-7229.6890744
CGACGGG8450.0225.9515244
TAAGCCA10600.0225.6261644
TCGAGGG1000.0221.0757344
ACGACAC2150.0205.6518444
CTATCAT5300.0185.8097844
ATAATTC651.1641532E-10185.5180844
GATAATC457.5252683E-7178.6470644
Produced by FastQC (version 0.11.5)