FastQCFastQC Report
Tue 24 May 2016
ERR840941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266828
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC86560.2028673290791192No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74750.17518868817772829No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA60700.14226024578445629No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA49330.11561281589039915No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT43140.10110555194631703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT19950.0212.0465944
CCGTCCA651.2732926E-11144.1689844
ATACGCT1400.0133.8711944
TAAGCCA10600.0128.8194644
ACCGTCA900.0115.72565543
ATTAGTC1550.0112.2790644
CAGGTTA2100.0108.3719244
TACAATA1350.099.1638444
TATACGC1300.098.96899443
TTACTGT2750.097.3608644
CCCATAC5250.091.79738644
CCTATCA21300.091.4683343
TCGTCAA507.421477E-1085.7731343
ACCACTC4700.085.4496944
CGTTCGA1800.085.0923943
TCGTTAT952.7643182E-884.55022444
TCACGTC952.7643182E-884.55022444
CGTATCT303.6779966E-881.1532442
CGTCTAA203.811359E-581.1532442
TATCGTT451.8189894E-1281.1532442
Produced by FastQC (version 0.11.5)