##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840941.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4266828 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.41586138461639 39.0 37.0 39.0 35.0 39.0 2 37.85516407035859 39.0 38.0 39.0 35.0 39.0 3 38.071859470313775 39.0 38.0 39.0 35.0 40.0 4 38.671178214823755 39.0 38.0 41.0 35.0 41.0 5 39.283715912617055 40.0 39.0 41.0 35.0 41.0 6 39.31932855038919 41.0 39.0 41.0 35.0 41.0 7 39.30769274036825 40.0 39.0 41.0 35.0 41.0 8 39.25047013847289 40.0 39.0 41.0 35.0 41.0 9 39.18749501971957 40.0 39.0 41.0 35.0 41.0 10 39.077116302789804 40.0 38.0 41.0 35.0 41.0 11 39.0024601413509 40.0 38.0 41.0 35.0 41.0 12 38.952949591593566 40.0 38.0 41.0 35.0 41.0 13 38.83249875551581 40.0 38.0 41.0 35.0 41.0 14 38.82755855169226 40.0 38.0 41.0 35.0 41.0 15 38.82352581355517 40.0 38.0 41.0 35.0 41.0 16 38.77649626373503 40.0 38.0 41.0 35.0 41.0 17 38.76580799601015 40.0 38.0 41.0 35.0 41.0 18 38.772246268187985 40.0 38.0 41.0 35.0 41.0 19 38.8113240093109 40.0 38.0 41.0 35.0 41.0 20 38.80740165762482 40.0 38.0 41.0 35.0 41.0 21 38.75720535254761 40.0 38.0 41.0 35.0 41.0 22 38.68400577665657 40.0 38.0 41.0 35.0 41.0 23 38.52430540907672 40.0 37.0 41.0 35.0 41.0 24 38.3408860164975 40.0 37.0 41.0 34.0 41.0 25 38.261777907541294 40.0 36.0 41.0 34.0 41.0 26 38.19559366348816 40.0 36.0 41.0 34.0 41.0 27 38.15662652613586 40.0 36.0 41.0 34.0 41.0 28 38.057557761662316 40.0 35.0 41.0 34.0 41.0 29 37.92568433445894 40.0 35.0 41.0 33.0 41.0 30 37.83794040201368 40.0 35.0 41.0 33.0 41.0 31 37.699847029945325 39.0 35.0 41.0 33.0 41.0 32 37.57786029874162 39.0 35.0 41.0 33.0 41.0 33 37.43111970760885 39.0 35.0 41.0 33.0 41.0 34 37.3406296727965 39.0 35.0 41.0 33.0 41.0 35 37.19492109744408 39.0 35.0 41.0 33.0 41.0 36 37.12333591539732 38.0 35.0 41.0 33.0 41.0 37 37.05379007160555 38.0 35.0 40.0 33.0 41.0 38 36.9766127338912 38.0 35.0 40.0 33.0 41.0 39 36.82776352857191 37.0 35.0 40.0 33.0 41.0 40 36.72382441762373 37.0 35.0 40.0 33.0 41.0 41 36.64412975869354 37.0 35.0 40.0 33.0 41.0 42 36.52812722225774 36.0 35.0 40.0 33.0 41.0 43 36.435371572660294 36.0 35.0 40.0 33.0 41.0 44 36.27574395975964 36.0 35.0 40.0 32.0 41.0 45 36.08527225988717 36.0 35.0 40.0 32.0 41.0 46 35.83244589466126 35.0 34.0 40.0 31.0 41.0 47 35.65005735133671 35.0 34.0 39.0 31.0 41.0 48 35.69769690929122 35.0 34.0 39.0 31.0 41.0 49 35.627000280462845 35.0 34.0 39.0 31.0 41.0 50 35.920389025718116 36.0 34.0 39.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 4.0 21 23.0 22 78.0 23 173.0 24 365.0 25 873.0 26 1543.0 27 3372.0 28 7188.0 29 14210.0 30 26761.0 31 48484.0 32 81395.0 33 141292.0 34 286349.0 35 240225.0 36 390004.0 37 580821.0 38 689784.0 39 840592.0 40 913291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 46.06707840109796 16.865666954468285 37.06725464443375 2 28.011018958345634 18.85304493173852 30.276003626112885 22.859932483802954 3 23.730298010606475 18.724987273918707 29.86185522359936 27.682859491875462 4 22.146732889162628 19.201969238038185 33.39612002171168 25.255177851087506 5 21.95249023396303 20.839602627525647 30.25181704066815 26.956090097843177 6 24.006404757820096 23.306915582254543 27.038118246153818 25.648561413771542 7 29.273736836825858 20.87576532262374 21.667407263662845 28.183090576887558 8 25.72754280228779 20.766035096797903 25.489825228483546 28.01659687243076 9 27.821393316065233 20.78944827398714 25.421132513426837 25.968025896520786 10 28.000425608906664 19.811110267393016 24.52913499208311 27.65932913161721 11 28.512539057116904 21.17184006479755 23.50436905354516 26.811251824540385 12 29.050432780510487 20.86730470504084 24.18187468536346 25.900387829085215 13 27.746888320785366 21.26680522392747 24.63150612117479 26.354800334112365 14 26.598283314912152 22.49975860287783 21.790965091632472 29.110992990577543 15 24.946705140211886 24.168656435178544 23.420044117081822 27.464594307527747 16 27.27761700260709 23.25830804522704 21.92952235243605 27.53455259972982 17 28.10635910329641 24.685410332921787 20.162846967349047 27.04538359643276 18 27.431126823017003 21.680930189827198 21.00440889578863 29.88353409136717 19 28.815902586183462 21.4257992119673 22.23347179684768 27.524826405001562 20 29.229277580441487 22.177927022134476 22.230706276418925 26.362089121005113 21 28.34824839435759 26.00311050738394 19.970526114481295 25.678114983777178 22 30.901808087881676 24.18025755901105 20.011704244933238 24.906230108174036 23 30.520939676968467 25.04516235479846 20.94677826244695 23.487119705786125 24 30.07144886083995 24.342345180072876 20.98816732242312 24.59803863666405 25 29.535799348890322 25.191519854643886 21.115369337044022 24.15731145942177 26 30.80818437631726 24.18681241479355 21.34898663659045 23.65601657229874 27 30.016807227680225 26.15457880104945 19.86906196687488 23.959552004395444 28 29.176493095787276 23.632768812622135 21.41474687190508 25.775991219685512 29 29.192790044220907 25.86911054841254 21.02763951378215 23.910459893584402 30 29.920864727894138 26.562496589415048 20.503648507358253 23.012990175332565 31 28.18583079481464 25.066393591853053 22.491991524658488 24.25578408867382 32 27.088624517081566 24.141215535271815 23.011602329558453 25.75855761808816 33 26.294345945433843 23.96505770022963 23.600015178070763 26.140581176265766 34 29.73385749852282 23.489596020541878 23.525697960427777 23.250848520507525 35 26.346156360735907 24.50960620639086 21.770446821115648 27.37379061175758 36 26.43112982875681 25.35010233420102 23.3464358539184 24.87233198312377 37 26.174252706685657 26.905431468417003 22.208700231703883 24.711615593193457 38 27.22152045874274 25.480237678296447 21.237261159936175 26.06098070302464 39 27.393100707002603 25.649265644585416 21.977192517712393 24.980441130699585 40 26.1956616362291 25.236028462286225 22.624325595952435 25.943984305532236 41 26.58752810367744 24.190992722420532 22.44074399997911 26.78073517392292 42 25.800275872374694 25.67715144804057 21.941272280456392 26.581300399128345 43 26.165953042424526 25.246644936710883 22.502696722297767 26.084705298566824 44 25.399641198789475 25.598415226914263 22.450112081347783 26.55183149294848 45 27.367873247531605 22.940687004783815 24.08820173795683 25.603238009727754 46 26.309842812451922 25.864210985178726 22.374961536489053 25.4509846658803 47 24.839219537587823 24.423649330623647 23.610365005697304 27.12676612609123 48 24.518359615909695 25.55715625005967 22.007671877356888 27.91681225667375 49 24.86140881749223 25.754491911639064 21.390735209189955 27.993364061678754 50 21.91058774587897 30.460077118108575 23.405204132925526 24.224131003086928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 68570.0 1 60679.5 2 51334.0 3 45224.5 4 36605.0 5 26909.5 6 17251.5 7 12891.0 8 13005.5 9 14074.5 10 14256.5 11 12668.0 12 10981.0 13 10916.0 14 11883.0 15 13168.5 16 14473.5 17 14432.5 18 13930.0 19 15972.5 20 19796.0 21 24702.5 22 28728.0 23 32275.0 24 35910.0 25 36658.5 26 36832.5 27 40421.5 28 44901.0 29 48195.5 30 50068.0 31 51865.0 32 57380.5 33 66923.5 34 80344.5 35 87208.5 36 86776.5 37 86925.0 38 86693.5 39 90697.0 40 101038.0 41 115324.0 42 132533.5 43 144378.0 44 146626.5 45 148483.5 46 154589.0 47 161206.0 48 176210.0 49 201544.0 50 230165.5 51 256958.0 52 269655.0 53 261775.0 54 256366.0 55 261571.0 56 267904.5 57 277295.0 58 287194.0 59 286700.5 60 277670.5 61 274760.0 62 273637.0 63 270699.5 64 262658.0 65 251252.0 66 235063.5 67 217056.5 68 196659.0 69 177049.5 70 165488.5 71 154697.0 72 144112.0 73 134815.0 74 121320.5 75 105801.0 76 94415.0 77 77137.0 78 60013.5 79 49619.5 80 39798.5 81 30895.5 82 24525.5 83 19677.0 84 15846.5 85 11884.5 86 8356.5 87 6936.5 88 5145.0 89 3474.0 90 1924.5 91 739.5 92 325.5 93 283.0 94 188.0 95 78.5 96 59.0 97 50.0 98 25.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 30061.0 25 23471.0 26 19862.0 27 22285.0 28 22885.0 29 25071.0 30 25659.0 31 28265.0 32 37136.0 33 29578.0 34 25751.0 35 22603.0 36 19869.0 37 24341.0 38 25564.0 39 24723.0 40 30134.0 41 28537.0 42 44621.0 43 65789.0 44 193413.0 45 377370.0 46 476484.0 47 548432.0 48 707931.0 49 752382.0 50 634611.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.023180588122534 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75989674860071 11.682009713679907 2 9.359773351926739 2.8122713065978475 3 3.0297574807095993 1.3654978131274647 4 1.4526880842428427 0.8729598171117594 5 0.8390983802019962 0.6302963248487845 6 0.5681459780637723 0.5121215777320532 7 0.431892814448526 0.45418826223208925 8 0.32873029838117196 0.3950859709894201 9 0.2616446202063441 0.3537660941343577 >10 2.724710750146456 9.871525960138355 >50 1.579015476917948 17.495793293292067 >100 1.5922701024119295 40.94534842295977 >500 0.04723771469421404 4.8676915195953985 >1k 0.024309467211057516 6.820088507790379 >5k 8.28731836740597E-4 0.9213554157703632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 8656 0.2028673290791192 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7475 0.17518868817772829 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 6070 0.14226024578445629 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 4933 0.11561281589039915 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT 4314 0.10110555194631703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02861610545351254 0.0 0.0 0.0 0.0 2 0.05519322550616055 0.0 0.0 0.0 0.0 3 0.07080201029898557 0.0 0.0 0.0 0.0 4 0.0871138935059018 0.0 0.0 0.0 0.0 5 0.1023008192502721 0.0 0.0 0.0 0.0 6 0.1297450940136326 0.0 0.0 0.0 0.0 7 0.14584604769632148 2.3436613803040574E-5 0.0 0.0 0.0 8 0.16265009979310158 2.3436613803040574E-5 0.0 0.0 0.0 9 0.1788447999310026 2.3436613803040574E-5 0.0 0.0 0.0 10 0.19389110599255466 2.3436613803040574E-5 0.0 0.0 0.0 11 0.20713279279127259 2.3436613803040574E-5 0.0 0.0 0.0 12 0.22196816932859725 2.3436613803040574E-5 0.0 0.0 0.0 13 0.23617075729323986 2.3436613803040574E-5 0.0 0.0 0.0 14 0.24917807795392738 2.3436613803040574E-5 0.0 0.0 0.0 15 0.26464624306393414 2.3436613803040574E-5 0.0 0.0 0.0 16 0.2784972818215311 2.3436613803040574E-5 0.0 0.0 0.0 17 0.2964497279946602 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 18 0.32035507407376157 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 19 0.335635746273344 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 20 0.35077579879010823 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 21 0.36682987924519106 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 22 0.3808918475270154 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 23 0.3967349984578708 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 24 0.41225003679548367 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 25 0.43074152508608265 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 26 0.44747526734145365 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 27 0.4642090095968246 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 28 0.4806615124865591 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 29 0.49938736691518854 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 30 0.5194491083305912 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 31 0.5357844281513106 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 32 0.5523775507238633 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 33 0.5697909547795224 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 34 0.585165373434317 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 35 0.5988054826676866 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 36 0.6118362399421772 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 37 0.6209062094839539 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 38 0.6271169121417597 2.3436613803040574E-5 0.0 0.0 2.3436613803040574E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCAT 1995 0.0 212.04659 44 CCGTCCA 65 1.2732926E-11 144.16898 44 ATACGCT 140 0.0 133.87119 44 TAAGCCA 1060 0.0 128.81946 44 ACCGTCA 90 0.0 115.725655 43 ATTAGTC 155 0.0 112.27906 44 CAGGTTA 210 0.0 108.37192 44 TACAATA 135 0.0 99.16384 44 TATACGC 130 0.0 98.968994 43 TTACTGT 275 0.0 97.36086 44 CCCATAC 525 0.0 91.797386 44 CCTATCA 2130 0.0 91.46833 43 TCGTCAA 50 7.421477E-10 85.77313 43 ACCACTC 470 0.0 85.44969 44 CGTTCGA 180 0.0 85.09239 43 TCGTTAT 95 2.7643182E-8 84.550224 44 TCACGTC 95 2.7643182E-8 84.550224 44 CGTATCT 30 3.6779966E-8 81.15324 42 CGTCTAA 20 3.811359E-5 81.15324 42 TATCGTT 45 1.8189894E-12 81.15324 42 >>END_MODULE